Restriction map for
pFN21AE2593
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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BamHI 225 GGATCC G^GATCC
BbsI 658 GAAGAC GAAGAC(2/6)
BcnI 201 CCCGG CC^[GC]GG
BfaI 358 CTAG C^TAG
BpmI 885 CTGGAG CTGGAG(16/14)
BpuAI 658 GAAGAC GAAGAC(2/6)
BsiEI 65 CGGCCG CG[GA][CT]^CG
BstYI 225 GGATCC [GA]^GATC[CT]
DraII 386 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 65 CGGCCG [CT]^GGCC[GA]
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
EcoO109I 386 AGGGCCT [GA]G^G[ACGT]CC[CT]
HapII 202 CCGG C^CGG
HinfI 342 GATTC G^A[ACGT]TC
HpaII 202 CCGG C^CGG
MaeI 358 CTAG C^TAG
MfeI 452 CAATTG C^AATTG
MflI 225 GGATCC [GA]^GATC[CT]
MspA1I 371 CAGCTG C[AC]G^C[GT]G
MspI 202 CCGG C^CGG
MunI 452 CAATTG C^AATTG
NciI 201 CCCGG CC^[GC]GG
NcoI 128 CCATGG C^CATGG
PvuII 371 CAGCTG CAG^CTG
SfaNI 959 GCATC GCATC(5/9)
SfcI 869 CTACAG C^T[GA][CT]AG
TfiI 342 GATTC G^A[AT]TC
TspRI 972 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 225 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanII 4 GAGCCC G[GA]GC[CT]^C
133 GAGCCC
BglI 70 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
196 GCCGCCCCGGC
BmyI 4 GAGCCC G[AGT]GC[ACT]^C
133 GAGCCC
Bsp1286I 4 GAGCCC G[AGT]GC[ACT]^C
133 GAGCCC
BsrI 229 CCAGT ACTGG(1/-1)
971 CCAGT
BstNI 72 CCAGG CC^[AT]GG
115 CCAGG
CfoI 21 GCGC GCG^C
150 GCGC
DpnII 99 GATC ^GATC
226 GATC
EcoRII 72 CCAGG ^CC[AT]GG
115 CCAGG
EcoT14I 128 CCATGG C^C[AT][AT]GG
935 CCAAGG
HhaI 21 GCGC GCG^C
150 GCGC
HinP1I 21 GCGC G^CGC
150 GCGC
MaeII 459 ACGT A^CGT
495 ACGT
MaeIII 61 GTTAC ^GT[ACGT]AC
286 GTAAC
MboI 99 GATC ^GATC
226 GATC
MvaI 72 CCAGG CC^[AT]GG
115 CCAGG
NdeII 99 GATC ^GATC
226 GATC
Sau3AI 99 GATC ^GATC
226 GATC
StyI 128 CCATGG C^C[AT][AT]GG
935 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BssKI Cfr13I
DdeI DsaI EarI Fnu4HI HaeIII
ItaI Ksp632I MboII MnlI MseI
MwoI NlaIII NlaIV Sau96I ScrFI
SmlI Tru9I TseI Tsp509I XcmI