Restriction map for
pFN21AE2593
(Linear) (Six-base) MAPSORT of: KIEE2593.seq Check: 2246 from: 1 to: 996
KIEE2593.orf
With 182 enzymes: SgfI *
November 2, 2015 11:21 ..
BamHI G'GATC_C
Cuts at: 0 225 996
Size: 225 771
BanII G_rGCy'C
Cuts at: 0 8 137 996
Size: 8 129 859
Fragments arranged by size:
859 129 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 665 996
Size: 665 331
BglI GCCn_nnn'nGGC
Cuts at: 0 76 202 996
Size: 76 126 794
Fragments arranged by size:
794 126 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 996
Size: 906 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 170 934 996
Size: 41 129 764 62
Fragments arranged by size:
764 129 62 41
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 697 700 703 706 718 746 749
Size: 682 15 3 3 3 12 28 3
Cuts at: 749 770 773 776 779 996
Size: 21 3 3 3 217
Fragments arranged by size:
682 217 28 21 15 12 3 3
3 3 3 3 3
BsiEI CG_ry'CG
Cuts at: 0 68 996
Size: 68 928
Bsp1286I G_dGCh'C
Cuts at: 0 8 137 996
Size: 8 129 859
Fragments arranged by size:
859 129 8
BstYI r'GATC_y
Cuts at: 0 225 996
Size: 225 771
BtgI C'CryG_G
Cuts at: 0 8 128 137 996
Size: 8 120 9 859
Fragments arranged by size:
859 120 9 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 528 996
Size: 528 468
EaeI y'GGCC_r
Cuts at: 0 65 996
Size: 65 931
EagI C'GGCC_G
Cuts at: 0 65 996
Size: 65 931
EarI CTCTTCn'nnn_
Cuts at: 0 659 668 695 782 788 803 809 847
Size: 659 9 27 87 6 15 6 38
Cuts at: 847 996
Size: 149
Fragments arranged by size:
659 149 87 38 27 15 9 6
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 996
Size: 906 90
EcoO109I rG'GnC_Cy
Cuts at: 0 387 996
Size: 387 609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 275 329 996
Size: 275 54 667
Fragments arranged by size:
667 275 54
MfeI C'AATT_G
Cuts at: 0 452 996
Size: 452 544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 158 996
Size: 29 129 838
Fragments arranged by size:
838 129 29
MspA1I CmG'CkG
Cuts at: 0 373 996
Size: 373 623
NcoI C'CATG_G
Cuts at: 0 128 996
Size: 128 868
PasI CC'CwG_GG
Cuts at: 0 72 996
Size: 72 924
PvuII CAG'CTG
Cuts at: 0 373 996
Size: 373 623
SfcI C'TryA_G
Cuts at: 0 869 996
Size: 869 127
SmlI C'TyrA_G
Cuts at: 0 56 185 913 996
Size: 56 129 728 83
Fragments arranged by size:
728 129 83 56
StyI C'CwwG_G
Cuts at: 0 128 935 996
Size: 128 807 61
Fragments arranged by size:
807 128 61
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 135 174 996
Size: 45 90 39 822
Fragments arranged by size:
822 90 45 39
Enzymes that do cut:
BamHI BanII BbsI BglI BpmI BpuEI BseRI BsiEI
Bsp1286I BstYI BtgI BtgZI EaeI EagI EarI Eco57MI
EcoO109I FalI MfeI MmeI MspA1I NcoI PasI PvuII
SfcI SmlI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI