Restriction map for
pFN21AB6501
(Circular) (Six-base) MAPSORT of: pFN21AB6501.seq Check: 6504 from: 1 to: 5779
pFN21AB6501 5779 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2259 3589 278
Size: 53 83 186 936 723 1330 2468
Fragments arranged by size:
2468 1330 936 723 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2511 2598 3122 1937
Size: 574 87 524 4594
Fragments arranged by size:
4594 574 524 87
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2392 2972 3842 5226 5494 1632
Size: 695 65 580 870 1384 268 1917
Fragments arranged by size:
1917 1384 870 695 580 268 65
AfeI AGC'GCT
Cuts at: 2363 2363
Size: 5779
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2281 4678 5655 1203
Size: 1078 2397 977 1327
Fragments arranged by size:
2397 1327 1078 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 2564
Size: 32 5747
AlwNI CAG_nnn'CTG
Cuts at: 3522 5094 3522
Size: 1572 4207
ApaI G_GGCC'C
Cuts at: 2692 2692
Size: 5779
ApaLI G'TGCA_C
Cuts at: 2192 3836 4992 2192
Size: 1644 1156 2979
Fragments arranged by size:
2979 1644 1156
ApoI r'AATT_y
Cuts at: 1493 2769 3073 3088 3314 3466 5575 1493
Size: 1276 304 15 226 152 2109 1697
Fragments arranged by size:
2109 1697 1276 304 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2627 3104 1870
Size: 73 72 612 477 4545
Fragments arranged by size:
4545 612 477 73 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2615 2648 2615
Size: 33 5746
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2083 3100 4555 618
Size: 325 217 627 224 72 1017 1455 1842
Fragments arranged by size:
1842 1455 1017 627 325 224 217 72
BanII G_rGCy'C
Cuts at: 1391 2406 2454 2692 3098 1391
Size: 1015 48 238 406 4072
Fragments arranged by size:
4072 1015 406 238 48
BbeI G_GCGC'C
Cuts at: 2015 2087 2015
Size: 72 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 928
Size: 971 317 4491
Fragments arranged by size:
4491 971 317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2030 4390
Size: 107 122 71 631 605 337 21 2360
Cuts at: 4390 136
Size: 1525
Fragments arranged by size:
2360 1525 631 605 337 122 107 71
21
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2196 2692 2970 3840 4996 1790
Size: 384 22 496 278 870 1156 2573
Fragments arranged by size:
2573 1156 870 496 384 278 22
BmgBI CAC'GTC
Cuts at: 1318 2349 1318
Size: 1031 4748
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 2935 3034
Size: 416 53 83 399 24 767 751 99
Cuts at: 3034 3510 4468 442
Size: 476 958 1753
Fragments arranged by size:
1753 958 767 751 476 416 399 99
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2422 4439 1239
Size: 1183 2017 2579
Fragments arranged by size:
2579 2017 1183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 3904 4769 5067 2219
Size: 1685 865 298 2931
Fragments arranged by size:
2931 1685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2063 4442 882
Size: 617 564 2379 2219
Fragments arranged by size:
2379 2219 617 564
BsaAI yAC'GTr
Cuts at: 493 1204 2284 2389 3570 493
Size: 711 1080 105 1181 2702
Fragments arranged by size:
2702 1181 1080 711 105
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2084 2256 3586
Size: 53 83 186 936 479 72 172 1330
Cuts at: 3586 3968 4603 275
Size: 382 635 1451
Fragments arranged by size:
1451 1330 936 635 479 382 186 172
83 72 53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 1178
Size: 30 165 30 1077 30 4447
Fragments arranged by size:
4447 1077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 1186
Size: 895 4884
BseYI C'CCAG_C
Cuts at: 2099 4982 5478 2099
Size: 2883 496 2400
Fragments arranged by size:
2883 2400 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2477 1163
Size: 573 741 4465
Fragments arranged by size:
4465 741 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3229 3990 5018 1540
Size: 275 186 33 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2196 2406 3840 3925 4996 2196
Size: 210 1434 85 1071 2979
Fragments arranged by size:
2979 1434 1071 210 85
BsmI GAATG_Cn'
Cuts at: 2247 3290 3383 2247
Size: 1043 93 4643
Fragments arranged by size:
4643 1043 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2196 2406 2454 2692 2970 3098
Size: 399 384 22 210 48 238 278 128
Cuts at: 3098 3840 3925 4996 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 278 238 210
128 85 48 22
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 2499 3134 844
Size: 1580 75 635 3489
Fragments arranged by size:
3489 1580 635 75
BsrBI CCG'CTC
Cuts at: 1394 1823 2916 3666 1394
Size: 429 1093 750 3507
Fragments arranged by size:
3507 1093 750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4423 4610 1700
Size: 110 2613 187 2869
Fragments arranged by size:
2869 2613 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 2840 3839 4851 2840
Size: 999 1012 3768
Fragments arranged by size:
3768 1012 999
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 3003 4583 3003
Size: 1580 4199
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2803 2593
Size: 210 5569
BstYI r'GATC_y
Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774
Size: 1137 367 385 17 1452 86 12 346
Cuts at: 5774 1972
Size: 1977
Fragments arranged by size:
1977 1452 1137 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2106 2106
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2166 2445 2665 2676 2919
Size: 552 939 30 132 279 220 11 243
Cuts at: 2919 513
Size: 3373
Fragments arranged by size:
3373 939 552 279 243 220 132 30
11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DraIII CAC_nnn'GTG
Cuts at: 2200 2200
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2160 2554
Size: 54 1425 50 275 18 201 129 394
Cuts at: 2554 3226 4115 5744 8
Size: 672 889 1629 43
Fragments arranged by size:
1629 1425 889 672 394 275 201 129
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3226 1537
Size: 275 219 1195 4090
Fragments arranged by size:
4090 1195 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 2459 3709 1353
Size: 1058 48 1250 3423
Fragments arranged by size:
3423 1250 1058 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 2078 2252 4366 4740 4886
Size: 1064 138 465 24 174 2114 374 146
Cuts at: 4886 387
Size: 1280
Fragments arranged by size:
2114 1280 1064 465 374 174 146 138
24
EcoICRI GAG'CTC
Cuts at: 2404 2404
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2392 2422 2972 3842 4439 5226
Size: 393 695 65 30 550 870 597 787
Cuts at: 5226 5494 1239
Size: 268 1524
Fragments arranged by size:
1524 870 787 695 597 550 393 268
65 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2270 2201
Size: 69 5710
EcoO109I rG'GnC_Cy
Cuts at: 2689 2689
Size: 5779
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 5779
FspI TGC'GCA
Cuts at: 2420 4285 2420
Size: 1865 3914
HaeII r_GCGC'y
Cuts at: 1678 2015 2087 2114 2365 2434 4926 1678
Size: 337 72 27 251 69 2492 2531
Fragments arranged by size:
2531 2492 337 251 72 69 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 4420 4452 4494
Size: 32 135 32 527 32 2289 32 42
Cuts at: 4494 4526 1373
Size: 32 2626
Fragments arranged by size:
2626 2289 527 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2848 3123 3369 669
Size: 988 281 910 275 246 3079
Fragments arranged by size:
3079 988 910 281 275 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2011 2083 2011
Size: 72 5707
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 4893 5077 1305
Size: 1716 1872 184 2007
Fragments arranged by size:
2007 1872 1716 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 2556 10
Size: 54 1425 343 330 394 3233
Fragments arranged by size:
3233 1425 394 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3737 4096 4255 518
Size: 648 1212 1359 359 159 2042
Fragments arranged by size:
2042 1359 1212 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 3195 3872 5020 5265 1606
Size: 430 1159 677 1148 245 2120
Fragments arranged by size:
2120 1159 1148 677 430 245
NarI GG'CG_CC
Cuts at: 2012 2084 2012
Size: 72 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 2031 3226 2031
Size: 1195 4584
NspI r_CATG'y
Cuts at: 1132 1229 2377 3137 4682 1132
Size: 97 1148 760 1545 2229
Fragments arranged by size:
2229 1545 1148 760 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2688 2688
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2145 2467 3131 830
Size: 1315 322 664 3478
Fragments arranged by size:
3478 1315 664 322
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
SacI G_AGCT'C
Cuts at: 2406 2406
Size: 5779
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2411 2459 2411
Size: 48 5731
SbfI CC_TGCA'GG
Cuts at: 2145 3131 2145
Size: 986 4793
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SexAI A'CCwGG_T
Cuts at: 2196 2196
Size: 5779
SfcI C'TryA_G
Cuts at: 826 1045 2141 2463 2512 3127 4262 4943 5134
Size: 219 1096 322 49 615 1135 681 191
Cuts at: 5134 826
Size: 1471
Fragments arranged by size:
1471 1135 1096 681 615 322 219 191
49
SfiI GGCCn_nnn'nGGCC
Cuts at: 2030 2030
Size: 5779
SfoI GGC'GCC
Cuts at: 2013 2085 2013
Size: 72 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 2017 3106 1872
Size: 145 1089 4545
Fragments arranged by size:
4545 1089 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 3883 4784 5046 820
Size: 197 926 291 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 291 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SrfI GCCC'GGGC
Cuts at: 2017 2017
Size: 5779
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2004 3218 513
Size: 552 939 1214 3074
Fragments arranged by size:
3074 1214 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4025 5737 96
Size: 274 80 33 51 1443 2048 1712 138
Fragments arranged by size:
2048 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 2661 2693 5739 5771 2602
Size: 32 27 32 3046 32 2610
Fragments arranged by size:
3046 2610 32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 2015 3104 1870
Size: 145 1089 4545
Fragments arranged by size:
4545 1089 145
XmnI GAAnn'nnTTC
Cuts at: 2649 2934 3908 2649
Size: 285 974 4520
Fragments arranged by size:
4520 974 285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2257 3587 276
Size: 53 83 186 936 723 1330 2468
Fragments arranged by size:
2468 1330 936 723 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SanDI SwaI XcmI