Restriction map for
pFN21AE6766
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp700I AviII AvrII BbeI BbvCI
BclI BfrI BglI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I CfoI
DraI DrdI Eco47III EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1170 GGTACC G^GTACC
AccI 1018 GTCTAC GT^[AC][GT]AC
AcsI 1327 GAATTT [GA]^AATT[CT]
AcyI 162 GACGCC G[GA]^CG[CT]C
AfaI 1171 GTAC GT^AC
ApoI 1327 GAATTT [GA]^AATT[CT]
Asp718I 1170 GGTACC G^GTACC
AspI 1261 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1748 CTCGGG C^[CT]CG[GA]G
BalI 1601 TGGCCA TGG^CCA
BanII 1570 GAGCCC G[GA]GC[CT]^C
BbrPI 871 CACGTG CAC^GTG
BbsI 22 GTCTTC GAAGAC(2/6)
BciVI 385 GGATAC GTATCC(6/5)
BcnI 1422 CCCGG CC^[GC]GG
BglII 129 AGATCT A^GATCT
BlpI 621 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 621 GCTGAGC GC^T[ACGT]AGC
BpuAI 22 GTCTTC GAAGAC(2/6)
BsaAI 871 CACGTG [CT]AC^GT[GA]
BsaHI 162 GACGCC G[GA]^CG[CT]C
BseRI 736 CTCCTC GAGGAG(10/8)
BsoBI 1748 CTCGGG C^[CT]CG[GA]G
BspDI 985 ATCGAT AT^CGAT
BsrBI 624 GAGCGG CCGCTC(-3/-3)
BsrFI 242 GCCGGT [GA]^CCGG[CT]
BssSI 751 CACGAG CACGAG(-5/-1)
BstAPI 1359 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1491 GGTTACC G^GT[ACGT]ACC
BstPI 1491 GGTTACC G^GT[ACGT]ACC
CelII 621 GCTGAGC GC^T[ACGT]AGC
Cfr10I 242 GCCGGT [GA]^CCGG[CT]
ClaI 985 ATCGAT AT^CGAT
CpoI 244 CGGTCCG CG^G[AT]CCG
EagI 1275 CGGCCG C^GGCCG
EclXI 1275 CGGCCG C^GGCCG
Eco52I 1275 CGGCCG C^GGCCG
EcoO65I 1491 GGTTACC G^GT[ACGT]ACC
Hin1I 162 GACGCC G[GA]^CG[CT]C
HincII 508 GTTGAC GT[CT]^[GA]AC
HindII 508 GTTGAC GT[CT]^[GA]AC
KpnI 1170 GGTACC GGTAC^C
MluNI 1601 TGGCCA TGG^CCA
MscI 1601 TGGCCA TGG^CCA
NciI 1422 CCCGG CC^[GC]GG
NcoI 959 CCATGG C^CATGG
PflFI 1261 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1438 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 871 CACGTG CAC^GTG
PmlI 871 CACGTG CAC^GTG
RsaI 1171 GTAC GT^AC
RsrII 244 CGGTCCG CG^G[AT]CCG
SapI 1515 GCTCTTC GCTCTTC(1/4)
SspI 91 AATATT AAT^ATT
StuI 336 AGGCCT AGG^CCT
Tth111I 1261 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1438 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 8 CCAGGAGACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 249 CGCG CG^CG
1138 CGCG
AflIII 306 ACGTGT A^C[GA][CT]GT
710 ACACGT
AhdI 14 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1387 GACAGTGGGTC
Alw44I 41 GTGCAC G^TGCAC
874 GTGCAC
AlwNI 513 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1439 CAGCTTCTG
AocI 999 CCTAAGG CC^T[ACGT]AGG
1255 CCTCAGG
ApaLI 41 GTGCAC G^TGCAC
874 GTGCAC
AspEI 14 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1387 GACAGTGGGTC
AspHI 41 GTGCAC G[AT]GC[AT]^C
874 GTGCAC
BamHI 790 GGATCC G^GATCC
1373 GGATCC
BcgI 174 CGACCGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1472 GCAGATGTTTCG
BpmI 702 CTCCAG CTGGAG(16/14)
1527 CTCCAG
BsgI 1357 CTGCAC GTGCAG(16/14)
577 GTGCAG
BsiEI 174 CGACCG CG[GA][CT]^CG
1275 CGGCCG
BsiHKAI 41 GTGCAC G[AT]GC[AT]^C
874 GTGCAC
BspMI 1355 ACCTGC ACCTGC(4/8)
1560 ACCTGC
BsrDI 591 CATTGC GCAATG(2/0)
146 GCAATG
BstUI 249 CGCG CG^CG
1138 CGCG
BstXI 1071 CCAACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1338 CCACTACTGTGG
Bsu36I 999 CCTAAGG CC^T[ACGT]AGG
1255 CCTCAGG
DraII 1532 GGGACCC [GA]G^G[ACGT]CC[CT]
1752 GGGTCCT
DsaI 893 CCACGG C^C[GA][CT]GG
959 CCATGG
Eam1105I 14 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1387 GACAGTGGGTC
Eco81I 999 CCTAAGG CC^T[ACGT]AGG
1255 CCTCAGG
EcoO109I 1532 GGGACCC [GA]G^G[ACGT]CC[CT]
1752 GGGTCCT
FauI 331 CCCGC CCCGC(4/6)
744 GCGGG
HgaI 162 GACGC GACGC(5/10)
1139 GCGTC
MslI 795 CATTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1339 CACTACTGTG
MspA1I 513 CAGCTG C[AC]G^C[GT]G
1175 CAGCTG
MvnI 249 CGCG CG^CG
1138 CGCG
PpuMI 1532 GGGACCC [GA]G^G[AT]CC[CT]
1752 GGGTCCT
PstI 231 CTGCAG CTGCA^G
1297 CTGCAG
PvuII 513 CAGCTG CAG^CTG
1175 CAGCTG
SfaNI 979 GATGC GCATC(5/9)
1231 GCATC
SfcI 231 CTGCAG C^T[GA][CT]AG
1297 CTGCAG
SphI 491 GCATGC GCATG^C
1065 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BfaI BmyI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraIII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII