(Circular) (Six-base) MAPSORT of: pFN21AE6766.seq Check: 3597 from: 1 to: 6475 pFN21AE6766 6475 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3574 3574 Size: 6475 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3024 3818 1937 Size: 1087 794 4594 Fragments arranged by size: 4594 1087 794 Acc65I G'GTAC_C Cuts at: 3175 3796 3175 Size: 621 5854 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3638 3710 4538 5922 6190 1632 Size: 2006 72 828 1384 268 1917 Fragments arranged by size: 2006 1917 1384 828 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 2311 2715 5374 6351 1203 Size: 1108 404 2659 977 1327 Fragments arranged by size: 2659 1327 1108 977 404 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 2024 3397 5204 2024 Size: 1373 1807 3295 Fragments arranged by size: 3295 1807 1373 AleI CACnn'nnGTG Cuts at: 1166 3348 1166 Size: 2182 4293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2238 2607 1184 Size: 1054 369 5052 Fragments arranged by size: 5052 1054 369 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2423 2455 2423 Size: 32 6443 AlwNI CAG_nnn'CTG Cuts at: 2523 3449 4218 5790 2523 Size: 926 769 1572 3208 Fragments arranged by size: 3208 1572 926 769 ApaLI G'TGCA_C Cuts at: 2046 2879 4532 5688 2046 Size: 833 1653 1156 2833 Fragments arranged by size: 2833 1653 1156 833 ApoI r'AATT_y Cuts at: 1493 3332 3784 4010 4162 6271 1493 Size: 1839 452 226 152 2109 1697 Fragments arranged by size: 2109 1839 1697 452 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 3753 3800 1870 Size: 73 1810 47 4545 Fragments arranged by size: 4545 1810 73 47 BamHI G'GATC_C Cuts at: 2795 3378 3805 4172 2795 Size: 583 427 367 5098 Fragments arranged by size: 5098 583 427 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2418 2915 3175 3796 5251 Size: 325 217 627 631 497 260 621 1455 Cuts at: 5251 618 Size: 1842 Fragments arranged by size: 1842 1455 631 627 621 497 325 260 217 BanII G_rGCy'C Cuts at: 1391 3579 3794 1391 Size: 2188 215 4072 Fragments arranged by size: 4072 2188 215 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2020 928 Size: 971 121 5383 Fragments arranged by size: 5383 971 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2168 2202 3466 3500 4666 4700 1903 Size: 34 231 34 1264 34 1166 34 3678 Fragments arranged by size: 3678 1264 1166 231 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2384 4367 5577 2384 Size: 1983 1210 3282 Fragments arranged by size: 3282 1983 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5086 136 Size: 107 122 71 631 605 3414 1525 Fragments arranged by size: 3414 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2134 6470 2134 Size: 4336 2139 BlpI GC'TnA_GC Cuts at: 2627 3892 2627 Size: 1265 5210 Bme1580I G_kGCm'C Cuts at: 1790 2050 2757 2883 4536 5692 1790 Size: 260 707 126 1653 1156 2573 Fragments arranged by size: 2573 1653 1156 707 260 126 BmgBI CAC'GTC Cuts at: 1318 2312 2718 3684 1318 Size: 994 406 966 4109 Fragments arranged by size: 4109 994 966 406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2740 4206 5164 Size: 416 53 83 399 24 1323 1466 958 Cuts at: 5164 442 Size: 1753 Fragments arranged by size: 1753 1466 1323 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2187 2977 3272 3760 2187 Size: 790 295 488 4902 Fragments arranged by size: 4902 790 488 295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2692 3517 5135 1239 Size: 1453 825 1618 2579 Fragments arranged by size: 2579 1618 1453 825 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4600 5465 5763 4600 Size: 865 298 5312 Fragments arranged by size: 5312 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2011 2644 3279 5138 882 Size: 617 512 633 635 1859 2219 Fragments arranged by size: 2219 1859 635 633 617 512 BsaAI yAC'GTr Cuts at: 493 1204 2878 4266 493 Size: 711 1674 1388 2702 Fragments arranged by size: 2702 1674 1388 711 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2168 4282 4664 5299 Size: 53 83 186 936 635 2114 382 635 Cuts at: 5299 275 Size: 1451 Fragments arranged by size: 2114 1451 936 635 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4908 5306 5580 5727 6138 4908 Size: 398 274 147 411 5245 Fragments arranged by size: 5245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 3338 3368 3480 Size: 30 165 30 1020 30 885 30 112 Cuts at: 3480 3510 1178 Size: 30 4143 Fragments arranged by size: 4143 1020 885 165 112 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2732 1186 Size: 1546 4929 BseYI C'CCAG_C Cuts at: 2634 3126 5678 6174 2634 Size: 492 2552 496 2935 Fragments arranged by size: 2935 2552 496 492 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 3347 1163 Size: 573 867 744 4291 Fragments arranged by size: 4291 867 744 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2182 3283 3925 4686 5714 1540 Size: 275 186 181 1101 642 761 1028 2301 Fragments arranged by size: 2301 1101 1028 761 642 275 186 181 BsiHKAI G_wGCw'C Cuts at: 2050 2883 4536 4621 5692 2050 Size: 833 1653 85 1071 2833 Fragments arranged by size: 2833 1653 1071 833 85 BsmI GAATG_Cn' Cuts at: 3986 4079 3986 Size: 93 6382 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2757 2883 3579 3794 4536 4621 Size: 399 260 707 126 696 215 742 85 Cuts at: 4621 5692 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 707 696 399 260 215 126 85 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 4364 6094 4364 Size: 1730 4745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3369 3574 3830 844 Size: 2525 205 256 3489 Fragments arranged by size: 3489 2525 256 205 BsrBI CCG'CTC Cuts at: 1394 1823 2631 4362 1394 Size: 429 808 1731 3507 Fragments arranged by size: 3507 1731 808 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2158 2595 4970 5144 65 Size: 1183 910 437 2375 174 1396 Fragments arranged by size: 2375 1396 1183 910 437 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2247 5119 5306 1700 Size: 110 437 2872 187 2869 Fragments arranged by size: 2872 2869 437 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 2756 4535 5547 2756 Size: 1779 1012 3684 Fragments arranged by size: 3684 1779 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3370 794 Size: 2576 3899 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 3496 1184 Size: 2312 4163 BstXI CCAn_nnnn'nTGG Cuts at: 3083 3350 3083 Size: 267 6208 BstYI r'GATC_y Cuts at: 1972 2134 2795 3378 3805 4172 4557 4574 6026 Size: 162 661 583 427 367 385 17 1452 Cuts at: 6026 6112 6124 6470 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 661 583 427 385 367 346 162 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3005 3261 3005 Size: 256 6219 BtgI C'CryG_G Cuts at: 513 1065 2898 2964 513 Size: 552 1833 66 4024 Fragments arranged by size: 4024 1833 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5736 Fragments arranged by size: 5736 683 56 BtsI GCAGTG_nn' Cuts at: 3991 4785 4805 3991 Size: 794 20 5661 Fragments arranged by size: 5661 794 20 ClaI AT'CG_AT Cuts at: 1554 2991 1554 Size: 1437 5038 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DraIII CAC_nnn'GTG Cuts at: 2077 3370 3421 2077 Size: 1293 51 5131 Fragments arranged by size: 5131 1293 51 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5482 809 Size: 507 4166 1802 Fragments arranged by size: 4166 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2525 3280 3606 Size: 54 1425 50 275 18 695 755 326 Cuts at: 3606 3922 4811 6440 8 Size: 316 889 1629 43 Fragments arranged by size: 1629 1425 889 755 695 326 316 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 3922 1537 Size: 275 1468 642 4090 Fragments arranged by size: 4090 1468 642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2946 3527 3729 3764 4405 1353 Size: 1593 581 202 35 641 3423 Fragments arranged by size: 3423 1593 641 581 202 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5062 5436 5582 387 Size: 1064 138 3473 374 146 1280 Fragments arranged by size: 3473 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2692 3517 3638 3710 4538 5135 5922 Size: 393 1060 825 121 72 828 597 787 Cuts at: 5922 6190 1239 Size: 268 1524 Fragments arranged by size: 1524 1060 828 825 787 597 393 268 121 72 EcoO109I rG'GnC_Cy Cuts at: 3538 3758 3538 Size: 220 6255 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2947 2947 Size: 6475 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 5622 1678 Size: 3944 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2262 2294 2301 Size: 32 135 32 438 32 220 32 7 Cuts at: 2301 2333 3383 3415 5116 5148 5190 5222 1373 Size: 32 1050 32 1701 32 42 32 2626 Fragments arranged by size: 2626 1701 1050 438 220 135 42 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2515 3819 4065 669 Size: 988 281 577 1304 246 3079 Fragments arranged by size: 3079 1304 988 577 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6475 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KpnI G_GTAC'C Cuts at: 3179 3800 3179 Size: 621 5854 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5589 5773 1305 Size: 4284 184 2007 Fragments arranged by size: 4284 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3608 10 Size: 54 1425 343 1776 2877 Fragments arranged by size: 2877 1776 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2804 3348 4433 4792 4951 518 Size: 648 1638 544 1085 359 159 2042 Fragments arranged by size: 2042 1638 1085 648 544 359 159 MspA1I CmG'CkG Cuts at: 1606 2520 3182 3891 4568 5716 5961 1606 Size: 914 662 709 677 1148 245 2120 Fragments arranged by size: 2120 1148 914 709 677 662 245 NcoI C'CATG_G Cuts at: 513 1065 2964 513 Size: 552 1899 4024 Fragments arranged by size: 4024 1899 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 2064 2500 3074 3833 5378 1132 Size: 97 835 436 574 759 1545 2229 Fragments arranged by size: 2229 1545 835 759 574 436 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 3449 1108 Size: 2341 4134 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PmlI CAC'GTG Cuts at: 2878 2878 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2423 2455 4544 4576 6057 6089 2423 Size: 32 2089 32 1481 32 2809 Fragments arranged by size: 2809 2089 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3538 3758 3538 Size: 220 6255 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PstI C_TGCA'G Cuts at: 830 2240 3306 3827 830 Size: 1410 1066 521 3478 Fragments arranged by size: 3478 1410 1066 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2520 3182 2520 Size: 662 5813 RsrII CG'GwC_CG Cuts at: 2250 2250 Size: 6475 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SanDI GG'GwC_CC Cuts at: 3538 3538 Size: 6475 SapI GCTCTTCn'nnn_ Cuts at: 3527 3527 Size: 6475 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 2236 3302 3823 4958 5639 5830 826 Size: 219 1191 1066 521 1135 681 191 1471 Fragments arranged by size: 1471 1191 1135 1066 681 521 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 3802 1872 Size: 1930 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4579 5480 5742 820 Size: 197 926 2636 901 262 1553 Fragments arranged by size: 2636 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 2500 3074 3833 1132 Size: 1368 574 759 3774 Fragments arranged by size: 3774 1368 759 574 SspI AAT'ATT Cuts at: 5 52 2098 4399 5 Size: 47 2046 2301 2081 Fragments arranged by size: 2301 2081 2046 47 StuI AGG'CCT Cuts at: 1517 2343 1517 Size: 826 5649 StyI C'CwwG_G Cuts at: 513 1065 2022 2422 2964 3384 3914 513 Size: 552 957 400 542 420 530 3074 Fragments arranged by size: 3074 957 552 542 530 420 400 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4502 4519 4672 4857 5302 325 Size: 1255 2922 17 153 185 445 1498 Fragments arranged by size: 2922 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4721 6433 96 Size: 274 80 33 51 1443 2744 1712 138 Fragments arranged by size: 2744 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6435 6467 6435 Size: 32 6443 Tth111I GACn'n_nGTC Cuts at: 1316 3269 1316 Size: 1953 4522 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2020 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 3800 1870 Size: 1930 4545 XmnI GAAnn'nnTTC Cuts at: 4604 4604 Size: 6475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI SacI SacII SexAI SfiI SfoI SrfI SwaI