Restriction map for
pFN21AE6750
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DraII DrdI Eam1105I Eco47III EcoO109I
EcoRI EcoRV FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1162 CGCG CG^CG
AcsI 1115 GAATTT [GA]^AATT[CT]
AfaI 61 GTAC GT^AC
Alw44I 255 GTGCAC G^TGCAC
AlwNI 33 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1746 CCTTAGG CC^T[ACGT]AGG
ApaLI 255 GTGCAC G^TGCAC
ApoI 1115 GAATTT [GA]^AATT[CT]
BbeI 75 GGCGCC GGCGC^C
BbsI 362 GTCTTC GAAGAC(2/6)
BclI 468 TGATCA T^GATCA
BfaI 1072 CTAG C^TAG
BglI 691 GCCCTTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 362 GTCTTC GAAGAC(2/6)
BsaWI 346 ACCGGA [AT]^CCGG[AT]
BsmBI 663 CGTCTC CGTCTC(1/5)
BsmI 515 GAATGC GAATGC(1/-1)
BsrDI 472 CATTGC GCAATG(2/0)
BstEII 325 GGTTACC G^GT[ACGT]ACC
BstPI 325 GGTTACC G^GT[ACGT]ACC
BstUI 1162 CGCG CG^CG
BstXI 1095 CCAGTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1746 CCTTAGG CC^T[ACGT]AGG
DraIII 1432 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1746 CCTTAGG CC^T[ACGT]AGG
EcoNI 204 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 325 GGTTACC G^GT[ACGT]ACC
EcoT22I 1360 ATGCAT ATGCA^T
FbaI 468 TGATCA T^GATCA
FseI 1200 GGCCGGCC GGCCGG^CC
HaeII 75 GGCGCC [GA]GCGC^[CT]
HincII 571 GTCAAC GT[CT]^[GA]AC
HindII 571 GTCAAC GT[CT]^[GA]AC
KasI 75 GGCGCC G^GCGCC
MaeI 1072 CTAG C^TAG
MvnI 1162 CGCG CG^CG
NaeI 1201 GCCGGC GCC^GGC
NarI 75 GGCGCC GG^CGCC
NgoMIV 1201 GCCGGC G^CCGGC
NsiI 1360 ATGCAT ATGCA^T
PflMI 603 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1672 CAGCTG CAG^CTG
RsaI 61 GTAC GT^AC
SfoI 75 GGCGCC GGC^GCC
SmlI 1548 CTCAAG C^T[CT][GA]AG
SspI 193 AATATT AAT^ATT
TfiI 1077 GAATC G^A[AT]TC
Tsp45I 1438 GTGAC ^GT[GC]AC
Tsp509I 1116 AATT ^AATT
Van91I 603 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 75 GGCGCC G[GA]^CG[CT]C
1440 GACGCC
AflIII 894 ACACGT A^C[GA][CT]GT
1238 ACGTGT
AvaII 344 GGACC G^G[AT]CC
1254 GGTCC
BamHI 995 GGATCC G^GATCC
1157 GGATCC
BanII 1219 GAGCCC G[GA]GC[CT]^C
1591 GAGCCC
BpmI 356 CTGGAG CTGGAG(16/14)
1695 CTGGAG
BsaHI 75 GGCGCC G[GA]^CG[CT]C
1440 GACGCC
BsgI 10 CTGCAC GTGCAG(16/14)
1381 CTGCAC
CfoI 76 GCGC GCG^C
542 GCGC
EarI 598 CTCTTC CTCTTC(1/4)
936 GAAGAG
EcoT14I 141 CCTTGG C^C[AT][AT]GG
614 CCTTGG
FauI 545 CCCGC CCCGC(4/6)
1264 GCGGG
HgaI 1440 GACGC GACGC(5/10)
838 GCGTC
HhaI 76 GCGC GCG^C
542 GCGC
Hin1I 75 GGCGCC G[GA]^CG[CT]C
1440 GACGCC
HinP1I 76 GCGC G^CGC
542 GCGC
HphI 535 GGTGA GGTGA(8/7)
1608 GGTGA
Ksp632I 598 CTCTTC CTCTTC(1/4)
936 GAAGAG
MslI 248 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1363 CATTATTGTG
SmaI 1168 CCCGGG CCC^GGG
1186 CCCGGG
StyI 141 CCTTGG C^C[AT][AT]GG
614 CCTTGG
XcmI 945 CCACGGGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1223 CCACGAGTCTGATGG
XmaCI 1168 CCCGGG C^CCGGG
1186 CCCGGG
XmaI 1168 CCCGGG C^CCGGG
1186 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
BanI BbvI BcnI BmyI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EclXI Eco52I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI XhoII