(Circular) (Six-base) MAPSORT of: pFN21AE6750.seq Check: 2247 from: 1 to: 6472 pFN21AE6750 6472 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4282 278 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 4535 5919 6187 1632 Size: 695 2208 1384 268 1917 Fragments arranged by size: 2208 1917 1384 695 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 2899 3243 5371 6348 1203 Size: 1696 344 2128 977 1327 Fragments arranged by size: 2128 1696 1327 977 344 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2275 2856 1184 Size: 1091 581 4800 Fragments arranged by size: 4800 1091 581 AlwNI CAG_nnn'CTG Cuts at: 2043 4215 5787 2043 Size: 2172 1572 2728 Fragments arranged by size: 2728 2172 1572 ApaLI G'TGCA_C Cuts at: 2260 4529 5685 2260 Size: 2269 1156 3047 Fragments arranged by size: 3047 2269 1156 ApoI r'AATT_y Cuts at: 1493 3120 3781 4007 4159 6268 1493 Size: 1627 661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1627 661 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2936 3173 3191 3797 1870 Size: 73 993 237 18 606 4545 Fragments arranged by size: 4545 993 606 237 73 18 BamHI G'GATC_C Cuts at: 3000 3162 3802 4169 3000 Size: 162 640 367 5303 Fragments arranged by size: 5303 640 367 162 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 3280 3473 3747 3793 Size: 325 217 627 293 1200 193 274 46 Cuts at: 3793 5248 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1200 627 325 293 274 217 193 46 BanII G_rGCy'C Cuts at: 1391 3228 3600 3791 1391 Size: 1837 372 191 4072 Fragments arranged by size: 4072 1837 372 191 BbeI G_GCGC'C Cuts at: 2084 2084 Size: 6472 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2360 928 Size: 971 461 5040 Fragments arranged by size: 5040 971 461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 2473 1548 Size: 925 5547 BfrBI ATG'CAT Cuts at: 3367 3367 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2702 5083 136 Size: 107 122 71 631 605 1030 2381 1525 Fragments arranged by size: 2381 1525 1030 631 605 122 107 71 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 2264 3174 3285 4533 5689 1790 Size: 474 910 111 1248 1156 2573 Fragments arranged by size: 2573 1248 1156 910 474 111 BmgBI CAC'GTC Cuts at: 1318 3019 3244 1318 Size: 1701 225 4546 Fragments arranged by size: 4546 1701 225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3094 3659 4203 Size: 416 53 83 399 24 1677 565 544 Cuts at: 4203 5161 442 Size: 958 1753 Fragments arranged by size: 1753 1677 958 565 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2382 3721 5132 1239 Size: 1143 1339 1411 2579 Fragments arranged by size: 2579 1411 1339 1143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3538 4597 5462 5760 3538 Size: 1059 865 298 4250 Fragments arranged by size: 4250 1059 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2456 3552 3563 3697 5135 882 Size: 617 957 1096 11 134 1438 2219 Fragments arranged by size: 2219 1438 1096 957 617 134 11 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 3446 4279 4661 Size: 53 83 186 936 548 1365 833 382 Cuts at: 4661 5296 275 Size: 635 1451 Fragments arranged by size: 1451 1365 936 833 635 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2351 4905 5303 5577 5724 6135 2351 Size: 2554 398 274 147 411 2688 Fragments arranged by size: 2688 2554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2828 2858 1178 Size: 30 165 30 1425 30 4792 Fragments arranged by size: 4792 1425 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 2705 2870 2990 1186 Size: 1516 3 165 120 4668 Fragments arranged by size: 4668 1516 165 120 3 BseYI C'CCAG_C Cuts at: 2553 3688 5675 6171 2553 Size: 1135 1987 496 2854 Fragments arranged by size: 2854 1987 1135 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2000 3371 1163 Size: 573 264 1371 4264 Fragments arranged by size: 4264 1371 573 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2117 2706 3207 3211 3922 4683 Size: 275 186 116 589 501 4 711 761 Cuts at: 4683 5711 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 711 589 501 275 186 116 4 BsiHKAI G_wGCw'C Cuts at: 2264 2743 3201 4533 4618 5689 2264 Size: 479 458 1332 85 1071 3047 Fragments arranged by size: 3047 1332 1071 479 458 85 BsmI GAATG_Cn' Cuts at: 2526 3983 4076 2526 Size: 1457 93 4922 Fragments arranged by size: 4922 1457 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2674 2674 Size: 6472 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2743 3174 3201 3228 3285 3600 Size: 399 474 479 431 27 27 57 315 Cuts at: 3600 3791 4533 4618 5689 1391 Size: 191 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 479 474 431 399 315 191 85 57 27 27 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3827 844 Size: 2983 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2476 4967 5141 65 Size: 1183 1228 2491 174 1396 Fragments arranged by size: 2491 1396 1228 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2680 3206 3470 5116 5303 1700 Size: 110 870 526 264 1646 187 2869 Fragments arranged by size: 2869 1646 870 526 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 2671 3229 3353 4532 5544 2671 Size: 558 124 1179 1012 3599 Fragments arranged by size: 3599 1179 1012 558 124 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6472 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 2330 1184 Size: 1146 5326 BstXI CCAn_nnnn'nTGG Cuts at: 3107 3107 Size: 6472 BstYI r'GATC_y Cuts at: 1972 3000 3162 3177 3802 4169 4554 4571 6023 Size: 1028 162 15 625 367 385 17 1452 Cuts at: 6023 6109 6121 6467 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1028 625 385 367 346 162 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 3752 3752 Size: 6472 BtgI C'CryG_G Cuts at: 513 1065 2950 3255 3707 513 Size: 552 1885 305 452 3278 Fragments arranged by size: 3278 1885 552 452 305 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2069 487 Size: 683 56 843 4890 Fragments arranged by size: 4890 843 683 56 BtsI GCAGTG_nn' Cuts at: 3988 4782 4802 3988 Size: 794 20 5658 Fragments arranged by size: 5658 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DraIII CAC_nnn'GTG Cuts at: 3442 3442 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5479 809 Size: 507 4163 1802 Fragments arranged by size: 4163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2703 3204 3208 Size: 54 1425 50 275 18 873 501 4 Cuts at: 3208 3304 3919 4808 6437 8 Size: 96 615 889 1629 43 Fragments arranged by size: 1629 1425 889 873 615 501 275 96 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2703 3204 3208 3919 1537 Size: 275 891 501 4 711 4090 Fragments arranged by size: 4090 891 711 501 275 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2609 2936 4402 1353 Size: 1256 327 1466 3423 Fragments arranged by size: 3423 1466 1256 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 2153 5059 5433 5579 387 Size: 1064 138 506 58 2906 374 146 1280 Fragments arranged by size: 2906 1280 1064 506 374 146 138 58 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2382 3721 4535 5132 5919 6187 Size: 393 695 55 1339 814 597 787 268 Cuts at: 6187 1239 Size: 1524 Fragments arranged by size: 1524 1339 814 787 695 597 393 268 55 EcoNI CCTnn'n_nnAGG Cuts at: 2213 2213 Size: 6472 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3579 3579 Size: 6472 FseI GG_CCGG'CC Cuts at: 3210 3210 Size: 6472 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 2084 5619 1678 Size: 406 3535 2531 Fragments arranged by size: 3535 2531 406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2828 2860 5113 Size: 32 135 32 534 32 690 32 2253 Cuts at: 5113 5145 5187 5219 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2253 690 534 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2578 3816 4062 669 Size: 988 281 640 1238 246 3079 Fragments arranged by size: 3079 1238 988 640 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KasI G'GCGC_C Cuts at: 2080 2080 Size: 6472 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2396 3039 5586 5770 1305 Size: 1091 643 2547 184 2007 Fragments arranged by size: 2547 2007 1091 643 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4650 Fragments arranged by size: 4650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2257 3372 4430 4789 4948 518 Size: 648 1091 1115 1058 359 159 2042 Fragments arranged by size: 2042 1115 1091 1058 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2553 3186 3652 3679 3888 4565 5713 5958 Size: 947 633 466 27 209 677 1148 245 Cuts at: 5958 1606 Size: 2120 Fragments arranged by size: 2120 1148 947 677 633 466 245 209 27 NaeI GCC'GGC Cuts at: 3208 3208 Size: 6472 NarI GG'CG_CC Cuts at: 2081 2081 Size: 6472 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5920 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NgoMIV G'CCGG_C Cuts at: 3206 3206 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NsiI A_TGCA'T Cuts at: 3369 3369 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 3830 5375 1132 Size: 97 2601 1545 2229 Fragments arranged by size: 2601 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6472 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2614 1108 Size: 1506 4966 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2577 2609 3587 3619 4541 4573 6054 6086 2577 Size: 32 978 32 922 32 1481 32 2963 Fragments arranged by size: 2963 1481 978 922 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 3126 4042 3126 Size: 916 5556 PstI C_TGCA'G Cuts at: 830 2835 3678 3717 3824 830 Size: 2005 843 39 107 3478 Fragments arranged by size: 3478 2005 843 107 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 3679 3679 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2831 3674 3713 3820 4955 5636 5827 Size: 219 1786 843 39 107 1135 681 191 Cuts at: 5827 826 Size: 1471 Fragments arranged by size: 1786 1471 1135 843 681 219 191 107 39 SfoI GGC'GCC Cuts at: 2082 2082 Size: 6472 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 3175 3193 3799 1872 Size: 1303 18 606 4545 Fragments arranged by size: 4545 1303 606 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 3553 4576 5477 5739 820 Size: 197 926 1610 1023 901 262 1553 Fragments arranged by size: 1610 1553 1023 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 2200 4396 5 Size: 47 2148 2196 2081 Fragments arranged by size: 2196 2148 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2146 2619 3911 513 Size: 552 1081 473 1292 3074 Fragments arranged by size: 3074 1292 1081 552 473 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2131 2528 2726 3113 4499 4516 4669 Size: 1255 551 397 198 387 1386 17 153 Cuts at: 4669 4854 5299 325 Size: 185 445 1498 Fragments arranged by size: 1498 1386 1255 551 445 397 387 198 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2065 4718 6430 Size: 274 80 33 51 1443 88 2653 1712 Cuts at: 6430 96 Size: 138 Fragments arranged by size: 2653 1712 1443 274 138 88 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 6432 6464 3056 Size: 32 3344 32 3064 Fragments arranged by size: 3344 3064 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6472 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2957 3235 2957 Size: 278 6194 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 3173 3191 3797 1870 Size: 1303 18 606 4545 Fragments arranged by size: 4545 1303 606 18 XmnI GAAnn'nnTTC Cuts at: 4601 4601 Size: 6472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4280 276 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstZ17I EcoICRI EcoO109I EcoRV FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI