(Linear) (Six-base) MAPSORT of: KIEE6750.seq Check: 3451 from: 1 to: 1767 KIEE6750.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 1767 Size: 322 1445 AflIII A'CryG_T Cuts at: 0 894 1238 1767 Size: 894 344 529 Fragments arranged by size: 894 529 344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 270 851 1767 Size: 270 581 916 Fragments arranged by size: 916 581 270 AlwNI CAG_nnn'CTG Cuts at: 0 38 1767 Size: 38 1729 ApaLI G'TGCA_C Cuts at: 0 255 1767 Size: 255 1512 ApoI r'AATT_y Cuts at: 0 1115 1767 Size: 1115 652 AvaI C'yCGr_G Cuts at: 0 931 1168 1186 1767 Size: 931 237 18 581 Fragments arranged by size: 931 581 237 18 BamHI G'GATC_C Cuts at: 0 995 1157 1767 Size: 995 162 610 Fragments arranged by size: 995 610 162 BanI G'GyrC_C Cuts at: 0 75 1275 1468 1742 1767 Size: 75 1200 193 274 25 Fragments arranged by size: 1200 274 193 75 25 BanII G_rGCy'C Cuts at: 0 1223 1595 1767 Size: 1223 372 172 Fragments arranged by size: 1223 372 172 BbeI G_GCGC'C Cuts at: 0 79 1767 Size: 79 1688 BbsI GAAGACnn'nnnn_ Cuts at: 0 355 1767 Size: 355 1412 BclI T'GATC_A Cuts at: 0 468 1767 Size: 468 1299 BfrBI ATG'CAT Cuts at: 0 1362 1767 Size: 1362 405 BglI GCCn_nnn'nGGC Cuts at: 0 697 1767 Size: 697 1070 Bme1580I G_kGCm'C Cuts at: 0 259 1169 1280 1767 Size: 259 910 111 487 Fragments arranged by size: 910 487 259 111 BmgBI CAC'GTC Cuts at: 0 1014 1239 1767 Size: 1014 225 528 Fragments arranged by size: 1014 528 225 BmrI ACTGGGnnnn_n' Cuts at: 0 1089 1654 1767 Size: 1089 565 113 Fragments arranged by size: 1089 565 113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 377 1716 1767 Size: 377 1339 51 Fragments arranged by size: 1339 377 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1533 1767 Size: 1533 234 BsaI GGTCTCn'nnnn_ Cuts at: 0 451 1547 1558 1692 1767 Size: 451 1096 11 134 75 Fragments arranged by size: 1096 451 134 75 11 BsaHI Gr'CG_yC Cuts at: 0 76 1441 1767 Size: 76 1365 326 Fragments arranged by size: 1365 326 76 BsaWI w'CCGG_w Cuts at: 0 346 1767 Size: 346 1421 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 823 853 1767 Size: 823 30 914 Fragments arranged by size: 914 823 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 697 700 865 985 1767 Size: 697 3 165 120 782 Fragments arranged by size: 782 697 165 120 3 BseYI C'CCAG_C Cuts at: 0 548 1683 1767 Size: 548 1135 84 Fragments arranged by size: 1135 548 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1366 1767 Size: 1366 401 BsiEI CG_ry'CG Cuts at: 0 112 701 1202 1206 1767 Size: 112 589 501 4 561 Fragments arranged by size: 589 561 501 112 4 BsiHKAI G_wGCw'C Cuts at: 0 259 738 1196 1767 Size: 259 479 458 571 Fragments arranged by size: 571 479 458 259 BsmI GAATG_Cn' Cuts at: 0 521 1767 Size: 521 1246 BsmBI CGTCTCn'nnnn_ Cuts at: 0 669 1767 Size: 669 1098 Bsp1286I G_dGCh'C Cuts at: 0 259 738 1169 1196 1223 1280 1595 1767 Size: 259 479 431 27 27 57 315 172 Fragments arranged by size: 479 431 315 259 172 57 27 27 BsrDI GCAATG_nn' Cuts at: 0 471 1767 Size: 471 1296 BsrFI r'CCGG_y Cuts at: 0 675 1201 1465 1767 Size: 675 526 264 302 Fragments arranged by size: 675 526 302 264 BssSI C'ACGA_G Cuts at: 0 666 1224 1348 1767 Size: 666 558 124 419 Fragments arranged by size: 666 558 419 124 BstEII G'GTnAC_C Cuts at: 0 325 1767 Size: 325 1442 BstXI CCAn_nnnn'nTGG Cuts at: 0 1102 1767 Size: 1102 665 BstYI r'GATC_y Cuts at: 0 995 1157 1172 1767 Size: 995 162 15 595 Fragments arranged by size: 995 595 162 15 Bsu36I CC'TnA_GG Cuts at: 0 1747 1767 Size: 1747 20 BtgI C'CryG_G Cuts at: 0 945 1250 1702 1767 Size: 945 305 452 65 Fragments arranged by size: 945 452 305 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 64 1767 Size: 64 1703 DraIII CAC_nnn'GTG Cuts at: 0 1437 1767 Size: 1437 330 EaeI y'GGCC_r Cuts at: 0 698 1199 1203 1299 1767 Size: 698 501 4 96 468 Fragments arranged by size: 698 501 468 96 4 EagI C'GGCC_G Cuts at: 0 698 1199 1203 1767 Size: 698 501 4 564 Fragments arranged by size: 698 564 501 4 EarI CTCTTCn'nnn_ Cuts at: 0 604 931 1767 Size: 604 327 836 Fragments arranged by size: 836 604 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 90 148 1767 Size: 90 58 1619 Fragments arranged by size: 1619 90 58 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 377 1716 1767 Size: 322 55 1339 51 Fragments arranged by size: 1339 322 55 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 208 1767 Size: 208 1559 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1574 1767 Size: 1574 193 FseI GG_CCGG'CC Cuts at: 0 1205 1767 Size: 1205 562 HaeII r_GCGC'y Cuts at: 0 79 1767 Size: 79 1688 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 101 133 823 855 1767 Size: 101 32 690 32 912 Fragments arranged by size: 912 690 101 32 32 HincII GTy'rAC Cuts at: 0 573 1767 Size: 573 1194 KasI G'GCGC_C Cuts at: 0 75 1767 Size: 75 1692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 391 1034 1767 Size: 391 643 733 Fragments arranged by size: 733 643 391 MslI CAynn'nnrTG Cuts at: 0 252 1367 1767 Size: 252 1115 400 Fragments arranged by size: 1115 400 252 MspA1I CmG'CkG Cuts at: 0 548 1181 1647 1674 1767 Size: 548 633 466 27 93 Fragments arranged by size: 633 548 466 93 27 NaeI GCC'GGC Cuts at: 0 1203 1767 Size: 1203 564 NarI GG'CG_CC Cuts at: 0 76 1767 Size: 76 1691 NgoMIV G'CCGG_C Cuts at: 0 1201 1767 Size: 1201 566 NsiI A_TGCA'T Cuts at: 0 1364 1767 Size: 1364 403 PflMI CCAn_nnn'nTGG Cuts at: 0 609 1767 Size: 609 1158 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 572 604 1582 1614 1767 Size: 572 32 978 32 153 Fragments arranged by size: 978 572 153 32 32 PsiI TTA'TAA Cuts at: 0 1121 1767 Size: 1121 646 PstI C_TGCA'G Cuts at: 0 830 1673 1712 1767 Size: 830 843 39 55 Fragments arranged by size: 843 830 55 39 PvuII CAG'CTG Cuts at: 0 1674 1767 Size: 1674 93 SfcI C'TryA_G Cuts at: 0 826 1669 1708 1767 Size: 826 843 39 59 Fragments arranged by size: 843 826 59 39 SfoI GGC'GCC Cuts at: 0 77 1767 Size: 77 1690 SmaI CCC'GGG Cuts at: 0 1170 1188 1767 Size: 1170 18 579 Fragments arranged by size: 1170 579 18 SmlI C'TyrA_G Cuts at: 0 1548 1767 Size: 1548 219 SspI AAT'ATT Cuts at: 0 195 1767 Size: 195 1572 StyI C'CwwG_G Cuts at: 0 141 614 1767 Size: 141 473 1153 Fragments arranged by size: 1153 473 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 126 523 721 1108 1767 Size: 126 397 198 387 659 Fragments arranged by size: 659 397 387 198 126 TatI w'GTAC_w Cuts at: 0 60 1767 Size: 60 1707 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1051 1083 1767 Size: 1051 32 684 Fragments arranged by size: 1051 684 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 952 1230 1767 Size: 952 278 537 Fragments arranged by size: 952 537 278 XmaI C'CCGG_G Cuts at: 0 1168 1186 1767 Size: 1168 18 581 Fragments arranged by size: 1168 581 18 Enzymes that do cut: AcuI AflIII AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BclI BfrBI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI EarI EciI Eco57MI EcoNI FalI FseI HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NsiI PflMI PpiI PsiI PstI PvuII SfcI SfoI SmaI SmlI SspI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BglII BlpI BmtI BplI Bpu10I BsaAI BsaBI BsiWI BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstZ17I BtsI ClaI CspCI DraI DrdI EcoICRI EcoO109I EcoRI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NspI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmnI ZraI