Restriction map for
pFN21AE2213
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiWI BsoBI BspDI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 297 GACGTC GACGT^C
AccII 628 CGCG CG^CG
AccIII 810 TCCGGA T^CCGGA
AgeI 341 ACCGGT A^CCGGT
ApaI 124 GGGCCC GGGCC^C
BalI 833 TGGCCA TGG^CCA
BamHI 4 GGATCC G^GATCC
BbsI 946 GAAGAC GAAGAC(2/6)
BpuAI 946 GAAGAC GAAGAC(2/6)
BseAI 810 TCCGGA T^CCGGA
BseRI 1042 CTCCTC GAGGAG(10/8)
BsgI 997 GTGCAG GTGCAG(16/14)
Bsp1407I 824 TGTACA T^GTACA
BspEI 810 TCCGGA T^CCGGA
BsrBI 454 GAGCGG CCGCTC(-3/-3)
BsrFI 341 ACCGGT [GA]^CCGG[CT]
BsrGI 824 TGTACA T^GTACA
BstUI 628 CGCG CG^CG
CfoI 167 GCGC GCG^C
Cfr10I 341 ACCGGT [GA]^CCGG[CT]
DraIII 625 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 672 ATGCAT ATGCA^T
FauI 412 CCCGC CCCGC(4/6)
HaeII 166 AGCGCC [GA]GCGC^[CT]
HhaI 167 GCGC GCG^C
HinP1I 167 GCGC G^CGC
HincII 496 GTCAAC GT[CT]^[GA]AC
HindII 496 GTCAAC GT[CT]^[GA]AC
HindIII 242 AAGCTT A^AGCTT
HphI 701 TCACC GGTGA(8/7)
MluNI 833 TGGCCA TGG^CCA
MroI 810 TCCGGA T^CCGGA
MscI 833 TGGCCA TGG^CCA
MspA1I 413 CCGCTG C[AC]G^C[GT]G
MvnI 628 CGCG CG^CG
NcoI 171 CCATGG C^CATGG
NsiI 672 ATGCAT ATGCA^T
PinAI 341 ACCGGT A^CCGGT
PshAI 282 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 418 GAAGAGC GCTCTTC(1/4)
SfaNI 1099 GATGC GCATC(5/9)
SphI 311 GCATGC GCATG^C
SspBI 824 TGTACA T^GTACA
StuI 20 AGGCCT AGG^CCT
TaqI 252 TCGA T^CGA
Tsp509I 927 AATT ^AATT
TthHB8I 252 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 297 GACGTC G[GA]^CG[CT]C
364 GACGCC
AlwNI 143 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
388 CAGTACCTG
BsaHI 297 GACGTC G[GA]^CG[CT]C
364 GACGCC
BsaWI 341 ACCGGT [AT]^CCGG[AT]
810 TCCGGA
BsmBI 226 GAGACG CGTCTC(1/5)
397 GAGACG
BsmI 670 GAATGC GAATGC(1/-1)
917 GCATTC
BspMI 875 ACCTGC ACCTGC(4/8)
151 GCAGGT
BstAPI 142 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
471 GCAAGCTCTGC
BstYI 4 GGATCC [GA]^GATC[CT]
887 GGATCT
DsaI 171 CCATGG C^C[GA][CT]GG
284 CCACGG
EaeI 431 CGGCCA [CT]^GGCC[GA]
833 TGGCCA
EcoT14I 171 CCATGG C^C[AT][AT]GG
555 CCTTGG
HapII 342 CCGG C^CGG
811 CCGG
HgaI 364 GACGC GACGC(5/10)
494 GCGTC
Hin1I 297 GACGTC G[GA]^CG[CT]C
364 GACGCC
HpaII 342 CCGG C^CGG
811 CCGG
MaeIII 300 GTCAC ^GT[ACGT]AC
551 GTGAC
MflI 4 GGATCC [GA]^GATC[CT]
887 GGATCT
MseI 35 TTAA T^TAA
1076 TTAA
MspI 342 CCGG C^CGG
811 CCGG
PflMI 387 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
907 CCAGGTGGTGG
PstI 149 CTGCAG CTGCA^G
478 CTGCAG
SacI 181 GAGCTC GAGCT^C
656 GAGCTC
StyI 171 CCATGG C^C[AT][AT]GG
555 CCTTGG
Tru9I 35 TTAA T^TAA
1076 TTAA
Tsp45I 300 GTCAC ^GT[GC]AC
551 GTGAC
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
533 CAGTG
Van91I 387 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
907 CCAGGTGGTGG
XcmI 47 CCAACGGCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
718 CCAGAAGTGGTGTGG
XhoII 4 GGATCC [GA]^GATC[CT]
887 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII ItaI Ksp632I MaeII
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfcI TseI