(Circular) (Six-base) MAPSORT of: pFN21AE2213.seq Check: 2571 from: 1 to: 5824 pFN21AE2213 5824 bp With 182 enzymes: SgfI * March 28, 2012 12:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2147 2889 2147 Size: 742 5082 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2306 3634 278 Size: 53 83 186 936 770 1328 2468 Fragments arranged by size: 2468 1328 936 770 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 2342 2442 3049 3887 5271 5539 1632 Size: 602 108 100 607 838 1384 268 1917 Fragments arranged by size: 1917 1384 838 607 602 268 108 100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 2346 4655 2346 Size: 2309 3515 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2551 1166 Size: 1385 4439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 2153 2398 3567 5139 2153 Size: 245 1169 1572 2838 Fragments arranged by size: 2838 1572 1169 245 ApaI G_GGCC'C Cuts at: 2133 2133 Size: 5824 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 3149 1870 Size: 73 1206 4545 Fragments arranged by size: 4545 1206 73 BamHI G'GATC_C Cuts at: 2009 3154 3521 2009 Size: 1145 367 4312 Fragments arranged by size: 4312 1145 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3145 4600 618 Size: 325 217 627 1358 1455 1842 Fragments arranged by size: 1842 1455 1358 627 325 217 BanII G_rGCy'C Cuts at: 1391 2133 2190 2665 3143 1391 Size: 742 57 475 478 4072 Fragments arranged by size: 4072 742 478 475 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2958 928 Size: 971 1059 3794 Fragments arranged by size: 3794 1059 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BfrBI ATG'CAT Cuts at: 2679 2679 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2133 3885 5041 1790 Size: 343 1752 1156 2573 Fragments arranged by size: 2573 1752 1156 343 BmgBI CAC'GTC Cuts at: 1318 2140 1318 Size: 822 5002 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2866 3555 4513 Size: 416 53 83 399 24 1449 689 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1449 958 689 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2515 2679 2906 4484 1239 Size: 1181 95 164 227 1578 2579 Fragments arranged by size: 2579 1578 1181 227 164 95 Bpu10I CC'TnA_GC Cuts at: 2670 2670 Size: 5824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2303 2370 3631 4013 Size: 53 83 186 936 770 67 1261 382 Cuts at: 4013 4648 275 Size: 635 1451 Fragments arranged by size: 1451 1261 936 770 635 382 186 83 67 53 BsaWI w'CCGG_w Cuts at: 2346 2815 4257 4655 4929 5076 5487 2346 Size: 469 1442 398 274 147 411 2683 Fragments arranged by size: 2683 1442 469 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5599 Fragments arranged by size: 5599 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3038 1186 Size: 1852 3972 BseYI C'CCAG_C Cuts at: 2095 2352 5027 5523 2095 Size: 257 2675 496 2396 Fragments arranged by size: 2675 2396 496 257 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3023 1163 Size: 573 1287 3964 Fragments arranged by size: 3964 1287 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2665 2876 3885 3970 5041 2190 Size: 475 211 1009 85 1071 2973 Fragments arranged by size: 2973 1071 1009 475 211 85 BsmI GAATG_Cn' Cuts at: 2681 2922 3335 3428 2681 Size: 241 413 93 5077 Fragments arranged by size: 5077 413 241 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2225 2396 2225 Size: 171 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2190 2665 2876 3143 3885 3970 Size: 399 343 57 475 211 267 742 85 Cuts at: 3970 5041 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 475 399 343 267 211 85 57 BspEI T'CCGG_A Cuts at: 2815 5487 2815 Size: 2672 3152 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2147 2889 3179 844 Size: 1303 742 290 3489 Fragments arranged by size: 3489 1303 742 290 BsrBI CCG'CTC Cuts at: 1394 1823 2461 3711 1394 Size: 429 638 1250 3507 Fragments arranged by size: 3507 1250 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2346 4468 4655 1700 Size: 110 536 2122 187 2869 Fragments arranged by size: 2869 2122 536 187 110 BsrGI T'GTAC_A Cuts at: 96 2829 96 Size: 2733 3091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2153 2482 794 Size: 1359 329 4136 Fragments arranged by size: 4136 1359 329 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2009 2892 3154 3521 3906 3923 5375 5461 Size: 37 883 262 367 385 17 1452 86 Cuts at: 5461 5473 5819 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 883 385 367 346 262 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2176 2289 513 Size: 552 939 172 113 4048 Fragments arranged by size: 4048 939 552 172 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2154 3340 4134 4154 2154 Size: 1186 794 20 3824 Fragments arranged by size: 3824 1186 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2896 2931 529 Size: 35 2332 35 3422 Fragments arranged by size: 3422 2332 35 35 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2635 2635 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2436 2838 3271 Size: 54 1425 50 275 18 606 402 433 Cuts at: 3271 4160 5789 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 606 433 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2418 2817 3058 3754 1353 Size: 731 334 399 241 696 3423 Fragments arranged by size: 3423 731 696 399 334 241 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2188 2663 2188 Size: 475 5349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2342 2420 2442 2515 2679 2906 Size: 393 602 108 78 22 73 164 227 Cuts at: 2906 3049 3887 4484 5271 5539 1239 Size: 143 838 597 787 268 1524 Fragments arranged by size: 1524 838 787 602 597 393 268 227 164 143 108 78 73 22 EcoO109I rG'GnC_Cy Cuts at: 2122 2129 2130 2845 2963 3024 2122 Size: 7 1 715 118 61 4922 Fragments arranged by size: 4922 715 118 61 7 1 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2767 2828 2767 Size: 61 5763 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2175 4971 1678 Size: 497 2796 2531 Fragments arranged by size: 2796 2531 497 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2688 2720 4465 4497 4539 Size: 32 135 32 1116 32 1745 32 42 Cuts at: 4539 4571 1373 Size: 32 2626 Fragments arranged by size: 2626 1745 1116 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2503 3168 3414 669 Size: 988 281 565 665 246 3079 Fragments arranged by size: 3079 988 665 565 281 246 HindIII A'AGCT_T Cuts at: 748 2247 748 Size: 1499 4325 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2840 10 Size: 54 1425 343 1008 2994 Fragments arranged by size: 2994 1425 1008 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2551 2999 3782 4141 4300 518 Size: 648 1038 347 448 783 359 159 2042 Fragments arranged by size: 2042 1038 783 648 448 359 347 159 MspA1I CmG'CkG Cuts at: 1606 2420 3240 3917 5065 5310 1606 Size: 814 820 677 1148 245 2120 Fragments arranged by size: 2120 1148 820 814 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2176 513 Size: 552 939 172 4161 Fragments arranged by size: 4161 939 552 172 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NsiI A_TGCA'T Cuts at: 2681 2681 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2320 2683 2998 3046 3182 4727 1132 Size: 97 1091 363 315 48 136 1545 2229 Fragments arranged by size: 2229 1545 1091 363 315 136 97 48 PasI CC'CwG_GG Cuts at: 1348 2126 1348 Size: 778 5046 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 2918 1108 Size: 1290 520 4014 Fragments arranged by size: 4014 1290 520 PfoI T'CCnGG_A Cuts at: 2760 2784 2943 2760 Size: 24 159 5641 Fragments arranged by size: 5641 159 24 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2845 2963 3024 2845 Size: 118 61 5645 Fragments arranged by size: 5645 118 61 PshAI GACnn'nnGTC Cuts at: 1613 2291 1613 Size: 678 5146 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2129 2129 Size: 5824 PstI C_TGCA'G Cuts at: 830 2158 2487 3176 830 Size: 1328 329 689 3478 Fragments arranged by size: 3478 1328 689 329 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 SacI G_AGCT'C Cuts at: 2190 2665 2190 Size: 475 5349 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SapI GCTCTTCn'nnn_ Cuts at: 2418 2418 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2154 2443 2465 2483 3172 4307 4988 Size: 219 1109 289 22 18 689 1135 681 Cuts at: 4988 5179 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1109 689 681 289 219 191 22 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 2320 3182 1132 Size: 1188 862 3774 Fragments arranged by size: 3774 1188 862 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2027 1517 Size: 510 5314 StyI C'CwwG_G Cuts at: 513 1065 2004 2176 2560 3263 513 Size: 552 939 172 384 703 3074 Fragments arranged by size: 3074 939 703 552 384 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3047 3851 3868 4021 4206 4651 325 Size: 1255 1467 804 17 153 185 445 1498 Fragments arranged by size: 1498 1467 1255 804 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 2829 4070 Size: 274 80 33 51 1443 348 504 1241 Cuts at: 4070 5782 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1241 504 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2730 2059 Size: 671 5153 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2304 3632 276 Size: 53 83 186 936 770 1328 2468 Fragments arranged by size: 2468 1328 936 770 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI