################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BpuAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I FauI FbaI FseI FspI HindIII HpaI HphI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I XbaI XhoII ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 150 GACGTC GACGT^C AccI 223 GTAGAC GT^[AC][GT]AC AcsI 250 GAATTC [GA]^AATT[CT] AfaI 102 GTAC GT^AC AhdI 307 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 454 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 250 GAATTC [GA]^AATT[CT] Asp700I 246 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 307 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 216 GGACC G^G[AT]CC BciVI 22 GTATCC GTATCC(6/5) BfaI 571 CTAG C^TAG BlpI 447 GCTCAGC GC^T[ACGT]AGC Bpu1102I 447 GCTCAGC GC^T[ACGT]AGC BsaI 412 GAGACC GGTCTC(1/5) BsaWI 500 ACCGGA [AT]^CCGG[AT] BseRI 442 GAGGAG GAGGAG(10/8) BsgI 481 GTGCAG GTGCAG(16/14) BsmAI 412 GAGAC GTCTC(1/5) BsmFI 148 GGGAC GGGAC(10/14) BsmI 286 GAATGC GAATGC(1/-1) BspMI 483 GCAGGT ACCTGC(4/8) BsrBI 120 GAGCGG CCGCTC(-3/-3) BsrI 126 ACTGG ACTGG(1/-1) BstF5I 617 GGATG GGATG(2/0) CelII 447 GCTCAGC GC^T[ACGT]AGC Cfr13I 216 GGACC G^G[ACGT]CC DdeI 448 CTCAG C^T[ACGT]AG EaeI 189 CGGCCA [CT]^GGCC[GA] Eam1105I 307 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 250 GAATTC G^AATTC EcoT22I 535 ATGCAT ATGCA^T FokI 617 GGATG GGATG(9/13) HaeII 455 AGCGCC [GA]GCGC^[CT] HaeIII 190 GGCC GG^CC HincII 598 GTCAAC GT[CT]^[GA]AC HindII 598 GTCAAC GT[CT]^[GA]AC MaeI 571 CTAG C^TAG MaeIII 56 GTAAC ^GT[ACGT]AC MspA1I 382 CAGCTG C[AC]G^C[GT]G NsiI 535 ATGCAT ATGCA^T PaeR7I 394 CTCGAG C^TCGAG PleI 401 GACTC GAGTC(4/5) PvuII 382 CAGCTG CAG^CTG RsaI 102 GTAC GT^AC SapI 4 GAAGAGC GCTCTTC(1/4) Sau96I 216 GGACC G^G[ACGT]CC SmlI 394 CTCGAG C^T[CT][GA]AG SnaBI 99 TACGTA TAC^GTA Tsp509I 251 AATT ^AATT TspRI 193 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 365 CCAACTTCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 394 CTCGAG C^TCGAG XmnI 246 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BanI 256 GGCACC G^G[CT][GA]CC 588 GGCACC BbvI 356 GCAGC GCAGC(8/12) 381 GCAGC BsaAI 99 TACGTA [CT]AC^GT[GA] 220 CACGTA BssHII 130 GCGCGC G^CGCGC 475 GCGCGC BstNI 168 CCAGG CC^[AT]GG 459 CCTGG EarI 403 CTCTTC CTCTTC(1/4) 4 GAAGAG EcoRII 168 CCAGG ^CC[AT]GG 459 CCTGG Ksp632I 403 CTCTTC CTCTTC(1/4) 4 GAAGAG MboII 4 GAAGA GAAGA(8/7) 404 TCTTC MvaI 168 CCAGG CC^[AT]GG 459 CCTGG NlaIII 435 CATG CATG^ 603 CATG PflMI 453 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 627 CCAACAGATGG SfaNI 534 GATGC GCATC(5/9) 618 GATGC SmaI 137 CCCGGG CCC^GGG 654 CCCGGG TaqI 395 TCGA T^CGA 407 TCGA TseI 356 GCAGC G^C[AT]GC 381 GCAGC TthHB8I 395 TCGA T^CGA 407 TCGA Van91I 453 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 627 CCAACAGATGG XmaCI 137 CCCGGG C^CCGGG 654 CCCGGG XmaI 137 CCCGGG C^CCGGG 654 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI BanII BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstUI Cac8I CfoI Fnu4HI HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MnlI MspI MvnI MwoI NciI NlaIV SacI ScrFI TfiI