(Circular) (Six-base) MAPSORT of: pFN21AE5231.seq Check: 2411 from: 1 to: 5380 pFN21AE5231 5380 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2159 3190 278 Size: 53 83 186 936 623 1031 2468 Fragments arranged by size: 2468 1031 936 623 186 83 53 AccI GT'mk_AC Cuts at: 1937 2229 2723 1937 Size: 292 494 4594 Fragments arranged by size: 4594 494 292 Acc65I G'GTAC_C Cuts at: 2701 2701 Size: 5380 AclI AA'CG_TT Cuts at: 1563 3507 3880 1563 Size: 1944 373 3063 Fragments arranged by size: 3063 1944 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3443 4827 5095 1632 Size: 1811 1384 268 1917 Fragments arranged by size: 1917 1811 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5183 AflIII A'CryG_T Cuts at: 1203 4279 5256 1203 Size: 3076 977 1327 Fragments arranged by size: 3076 1327 977 AgeI A'CCGG_T Cuts at: 4211 4211 Size: 5380 AhdI GACnn_n'nnGTC Cuts at: 2317 4109 2317 Size: 1792 3588 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5380 AlwNI CAG_nnn'CTG Cuts at: 2464 3123 4695 2464 Size: 659 1572 3149 Fragments arranged by size: 3149 1572 659 ApaLI G'TGCA_C Cuts at: 3437 4593 3437 Size: 1156 4224 ApoI r'AATT_y Cuts at: 1493 2255 2689 2915 3067 5176 1493 Size: 762 434 226 152 2109 1697 Fragments arranged by size: 2109 1697 762 434 226 152 AseI AT'TA_AT Cuts at: 160 1032 3934 160 Size: 872 2902 1606 Fragments arranged by size: 2902 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 AvaI C'yCGr_G Cuts at: 1870 1943 2142 2399 2659 2705 1870 Size: 73 199 257 260 46 4545 Fragments arranged by size: 4545 260 257 199 73 46 BamHI G'GATC_C Cuts at: 2710 3077 2710 Size: 367 5013 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 2593 2701 4156 618 Size: 325 217 627 474 332 108 1455 1842 Fragments arranged by size: 1842 1455 627 474 332 325 217 108 BanII G_rGCy'C Cuts at: 1391 2016 2400 2454 2472 2699 1391 Size: 625 384 54 18 227 4072 Fragments arranged by size: 4072 625 384 227 54 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3571 3605 1903 Size: 34 1634 34 3678 Fragments arranged by size: 3678 1634 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2038 3272 4482 2038 Size: 1234 1210 2936 Fragments arranged by size: 2936 1234 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5380 BfrBI ATG'CAT Cuts at: 2542 2542 Size: 5380 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3991 136 Size: 107 122 71 631 605 2319 1525 Fragments arranged by size: 2319 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5375 5375 Size: 5380 BlpI GC'TnA_GC Cuts at: 2453 2797 2453 Size: 344 5036 Bme1580I G_kGCm'C Cuts at: 1790 2564 3441 4597 1790 Size: 774 877 1156 2573 Fragments arranged by size: 2573 1156 877 774 BmgBI CAC'GTC Cuts at: 1318 2314 1318 Size: 996 4384 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 4069 442 Size: 416 53 83 399 24 1694 958 1753 Fragments arranged by size: 1753 1694 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5380 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2665 2665 Size: 5380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2402 2686 4040 1239 Size: 917 246 284 1354 2579 Fragments arranged by size: 2579 1354 917 284 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3505 4370 4668 3505 Size: 865 298 4217 Fragments arranged by size: 4217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2411 4043 882 Size: 617 912 1632 2219 Fragments arranged by size: 2219 1632 912 617 BsaAI yAC'GTr Cuts at: 493 1204 2106 2227 3171 493 Size: 711 902 121 944 2702 Fragments arranged by size: 2702 944 902 711 121 BsaBI GATnn'nnATC Cuts at: 5374 5374 Size: 5380 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2156 2433 2550 2580 Size: 53 83 186 936 623 277 117 30 Cuts at: 2580 3187 3569 4204 275 Size: 607 382 635 1451 Fragments arranged by size: 1451 936 635 623 607 382 277 186 117 83 53 30 BsaWI w'CCGG_w Cuts at: 2505 3813 4211 4485 4632 5043 2505 Size: 1308 398 274 147 411 2842 Fragments arranged by size: 2842 1308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5155 Fragments arranged by size: 5155 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2462 1186 Size: 1276 4104 BseYI C'CCAG_C Cuts at: 2149 4583 5079 2149 Size: 2434 496 2450 Fragments arranged by size: 2450 2434 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 1163 Size: 573 771 4036 Fragments arranged by size: 4036 771 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2830 3591 4619 1540 Size: 275 186 829 761 1028 2301 Fragments arranged by size: 2301 1028 829 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2400 2454 3441 3526 4597 2016 Size: 384 54 987 85 1071 2799 Fragments arranged by size: 2799 1071 987 384 85 54 BsmI GAATG_Cn' Cuts at: 2297 2891 2984 2297 Size: 594 93 4693 Fragments arranged by size: 4693 594 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2400 2454 2472 2564 2699 3441 Size: 399 226 384 54 18 92 135 742 Cuts at: 3441 3526 4597 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 384 226 135 92 85 54 18 BspEI T'CCGG_A Cuts at: 5043 5043 Size: 5380 BspHI T'CATG_A Cuts at: 3269 4999 3269 Size: 1730 3650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2479 2735 844 Size: 1635 256 3489 Fragments arranged by size: 3489 1635 256 BsrBI CCG'CTC Cuts at: 1394 1823 2127 3267 1394 Size: 429 304 1140 3507 Fragments arranged by size: 3507 1140 429 304 BsrDI GCAATG_nn' Cuts at: 65 1248 3875 4049 65 Size: 1183 2627 174 1396 Fragments arranged by size: 2627 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4024 4211 1700 Size: 110 2214 187 2869 Fragments arranged by size: 2869 2214 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5380 BssHII G'CGCG_C Cuts at: 1925 2135 2480 1925 Size: 210 345 4825 Fragments arranged by size: 4825 345 210 BssSI C'ACGA_G Cuts at: 3440 4452 3440 Size: 1012 4368 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5380 BstBI TT'CG_AA Cuts at: 4184 4184 Size: 5380 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5380 BstYI r'GATC_y Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375 Size: 738 367 385 17 1452 86 12 346 Cuts at: 5375 1972 Size: 1977 Fragments arranged by size: 1977 1452 738 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3889 Fragments arranged by size: 3889 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4641 Fragments arranged by size: 4641 683 56 BtsI GCAGTG_nn' Cuts at: 2197 2896 3690 3710 2197 Size: 699 794 20 3867 Fragments arranged by size: 3867 794 699 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5345 DraI TTT'AAA Cuts at: 2684 2684 Size: 5380 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4387 809 Size: 507 3071 1802 Fragments arranged by size: 3071 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 2827 3716 Size: 54 1425 50 275 18 364 633 889 Cuts at: 3716 5345 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 633 364 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2827 1537 Size: 275 1015 4090 Fragments arranged by size: 4090 1015 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2414 3310 1353 Size: 651 410 896 3423 Fragments arranged by size: 3423 896 651 410 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2082 2088 2097 3967 4341 4487 Size: 1064 138 493 6 9 1870 374 146 Cuts at: 4487 387 Size: 1280 Fragments arranged by size: 1870 1280 1064 493 374 146 138 9 6 EcoICRI GAG'CTC Cuts at: 2014 2398 2452 2014 Size: 384 54 4942 Fragments arranged by size: 4942 384 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2402 2686 3443 4040 4827 5095 Size: 393 524 246 284 757 597 787 268 Cuts at: 5095 1239 Size: 1524 Fragments arranged by size: 1524 787 757 597 524 393 284 268 246 EcoRI G'AATT_C Cuts at: 2255 2689 3067 2255 Size: 434 378 4568 Fragments arranged by size: 4568 434 378 FspI TGC'GCA Cuts at: 3886 3886 Size: 5380 HaeII r_GCGC'y Cuts at: 1678 2464 4527 1678 Size: 786 2063 2531 Fragments arranged by size: 2531 2063 786 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 4021 4053 4095 Size: 32 135 32 731 32 1686 32 42 Cuts at: 4095 4127 1373 Size: 32 2626 Fragments arranged by size: 2626 1686 731 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2605 2724 2970 669 Size: 988 281 667 119 246 3079 Fragments arranged by size: 3079 988 667 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 5380 HpaI GTT'AAC Cuts at: 2970 2970 Size: 5380 KpnI G_GTAC'C Cuts at: 2705 2705 Size: 5380 MfeI C'AATT_G Cuts at: 2979 2979 Size: 5380 MluI A'CGCG_T Cuts at: 5256 5256 Size: 5380 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 2301 2656 4494 4678 1305 Size: 834 162 355 1838 184 2007 Fragments arranged by size: 2007 1838 834 355 184 162 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3558 Fragments arranged by size: 3558 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3338 3697 3856 518 Size: 648 2172 359 159 2042 Fragments arranged by size: 2172 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2389 2796 3473 4621 4866 1606 Size: 783 407 677 1148 245 2120 Fragments arranged by size: 2120 1148 783 677 407 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3889 Fragments arranged by size: 3889 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5380 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5380 NotI GC'GGCC_GC Cuts at: 2827 2827 Size: 5380 NsiI A_TGCA'T Cuts at: 2544 2544 Size: 5380 NspI r_CATG'y Cuts at: 1132 1229 2738 4283 1132 Size: 97 1509 1545 2229 Fragments arranged by size: 2229 1545 1509 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5380 PciI A'CATG_T Cuts at: 4279 4279 Size: 5380 PflMI CCAn_nnn'nTGG Cuts at: 1108 2464 2638 1108 Size: 1356 174 3850 Fragments arranged by size: 3850 1356 174 PfoI T'CCnGG_A Cuts at: 2172 2172 Size: 5380 PmeI GTTT'AAAC Cuts at: 2684 2684 Size: 5380 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3449 3481 4962 4994 3449 Size: 32 1481 32 3835 Fragments arranged by size: 3835 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5380 PsiI TTA'TAA Cuts at: 2950 2950 Size: 5380 PspXI vC'TCGA_Gb Cuts at: 2399 2399 Size: 5380 PstI C_TGCA'G Cuts at: 830 2732 830 Size: 1902 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5380 PvuII CAG'CTG Cuts at: 2389 2389 Size: 5380 SacI G_AGCT'C Cuts at: 2016 2400 2454 2016 Size: 384 54 4942 Fragments arranged by size: 4942 384 54 SalI G'TCGA_C Cuts at: 1936 2722 1936 Size: 786 4594 SapI GCTCTTCn'nnn_ Cuts at: 2004 2004 Size: 5380 SbfI CC_TGCA'GG Cuts at: 2732 2732 Size: 5380 ScaI AGT'ACT Cuts at: 3628 5340 3628 Size: 1712 3668 SfcI C'TryA_G Cuts at: 826 1045 2728 3863 4544 4735 826 Size: 219 1683 1135 681 191 1471 Fragments arranged by size: 1683 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5380 SmaI CCC'GGG Cuts at: 1872 2144 2661 2707 1872 Size: 272 517 46 4545 Fragments arranged by size: 4545 517 272 46 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 3484 4385 4647 820 Size: 197 926 456 1085 901 262 1553 Fragments arranged by size: 1553 1085 926 901 456 262 197 SnaBI TAC'GTA Cuts at: 493 2106 3171 493 Size: 1613 1065 2702 Fragments arranged by size: 2702 1613 1065 SpeI A'CTAG_T Cuts at: 152 152 Size: 5380 SphI G_CATG'C Cuts at: 1132 2738 1132 Size: 1606 3774 SrfI GCCC'GGGC Cuts at: 2661 2661 Size: 5380 SspI AAT'ATT Cuts at: 5 52 3304 5 Size: 47 3252 2081 Fragments arranged by size: 3252 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5380 StyI C'CwwG_G Cuts at: 513 1065 2004 2819 513 Size: 552 939 815 3074 Fragments arranged by size: 3074 939 815 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3407 3424 3577 3762 4207 325 Size: 1255 1827 17 153 185 445 1498 Fragments arranged by size: 1827 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3626 5338 96 Size: 274 80 33 51 1443 1649 1712 138 Fragments arranged by size: 1712 1649 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5340 5372 5340 Size: 32 5348 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5380 XbaI T'CTAG_A Cuts at: 2716 2716 Size: 5380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2377 Size: 5380 XhoI C'TCGA_G Cuts at: 1943 2399 1943 Size: 456 4924 XmaI C'CCGG_G Cuts at: 1870 2142 2659 2705 1870 Size: 272 517 46 4545 Fragments arranged by size: 4545 517 272 46 XmnI GAAnn'nnTTC Cuts at: 2255 3509 2255 Size: 1254 4126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2157 3188 276 Size: 53 83 186 936 623 1031 2468 Fragments arranged by size: 2468 1031 936 623 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI