Restriction map for
pFN21AE5231
(Linear) (Six-base) MAPSORT of: KIEE5231.seq Check: 7239 from: 1 to: 675
KIEE5231.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AatII G_ACGT'C
Cuts at: 0 154 675
Size: 154 521
AccI GT'mk_AC
Cuts at: 0 224 675
Size: 224 451
AhdI GACnn_n'nnGTC
Cuts at: 0 312 675
Size: 312 363
AlwNI CAG_nnn'CTG
Cuts at: 0 459 675
Size: 459 216
ApoI r'AATT_y
Cuts at: 0 250 675
Size: 250 425
AvaI C'yCGr_G
Cuts at: 0 137 394 654 675
Size: 137 257 260 21
Fragments arranged by size:
260 257 137 21
BanI G'GyrC_C
Cuts at: 0 256 588 675
Size: 256 332 87
Fragments arranged by size:
332 256 87
BanII G_rGCy'C
Cuts at: 0 11 395 449 467 675
Size: 11 384 54 18 208
Fragments arranged by size:
384 208 54 18 11
BciVI GTATCCnnnnn_n'
Cuts at: 0 33 675
Size: 33 642
BfrBI ATG'CAT
Cuts at: 0 537 675
Size: 537 138
BlpI GC'TnA_GC
Cuts at: 0 448 675
Size: 448 227
Bme1580I G_kGCm'C
Cuts at: 0 559 675
Size: 559 116
BmgBI CAC'GTC
Cuts at: 0 309 675
Size: 309 366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 660 675
Size: 660 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 397 675
Size: 151 246 278
Fragments arranged by size:
278 246 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 406 675
Size: 406 269
BsaAI yAC'GTr
Cuts at: 0 101 222 675
Size: 101 121 453
Fragments arranged by size:
453 121 101
BsaHI Gr'CG_yC
Cuts at: 0 151 428 545 575 675
Size: 151 277 117 30 100
Fragments arranged by size:
277 151 117 100 30
BsaWI w'CCGG_w
Cuts at: 0 500 675
Size: 500 175
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 675
Size: 457 218
BseYI C'CCAG_C
Cuts at: 0 144 675
Size: 144 531
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 675
Size: 502 173
BsiHKAI G_wGCw'C
Cuts at: 0 11 395 449 675
Size: 11 384 54 226
Fragments arranged by size:
384 226 54 11
BsmI GAATG_Cn'
Cuts at: 0 292 675
Size: 292 383
Bsp1286I G_dGCh'C
Cuts at: 0 11 395 449 467 559 675
Size: 11 384 54 18 92 116
Fragments arranged by size:
384 116 92 54 18 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 675
Size: 474 201
BsrBI CCG'CTC
Cuts at: 0 122 675
Size: 122 553
BssHII G'CGCG_C
Cuts at: 0 130 475 675
Size: 130 345 200
Fragments arranged by size:
345 200 130
BtsI GCAGTG_nn'
Cuts at: 0 192 675
Size: 192 483
EaeI y'GGCC_r
Cuts at: 0 189 675
Size: 189 486
EarI CTCTTCn'nnn_
Cuts at: 0 409 675
Size: 409 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 77 83 92 675
Size: 77 6 9 583
Fragments arranged by size:
583 77 9 6
EcoICRI GAG'CTC
Cuts at: 0 9 393 447 675
Size: 9 384 54 228
Fragments arranged by size:
384 228 54 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 397 675
Size: 151 246 278
Fragments arranged by size:
278 246 151
EcoRI G'AATT_C
Cuts at: 0 250 675
Size: 250 425
HaeII r_GCGC'y
Cuts at: 0 459 675
Size: 459 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 675
Size: 298 32 345
Fragments arranged by size:
345 298 32
HincII GTy'rAC
Cuts at: 0 600 675
Size: 600 75
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 296 651 675
Size: 134 162 355 24
Fragments arranged by size:
355 162 134 24
MspA1I CmG'CkG
Cuts at: 0 384 675
Size: 384 291
NsiI A_TGCA'T
Cuts at: 0 539 675
Size: 539 136
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 633 675
Size: 459 174 42
Fragments arranged by size:
459 174 42
PfoI T'CCnGG_A
Cuts at: 0 167 675
Size: 167 508
PspXI vC'TCGA_Gb
Cuts at: 0 394 675
Size: 394 281
PvuII CAG'CTG
Cuts at: 0 384 675
Size: 384 291
SacI G_AGCT'C
Cuts at: 0 11 395 449 675
Size: 11 384 54 226
Fragments arranged by size:
384 226 54 11
SmaI CCC'GGG
Cuts at: 0 139 656 675
Size: 139 517 19
Fragments arranged by size:
517 139 19
SmlI C'TyrA_G
Cuts at: 0 394 675
Size: 394 281
SnaBI TAC'GTA
Cuts at: 0 101 675
Size: 101 574
SrfI GCCC'GGGC
Cuts at: 0 656 675
Size: 656 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 675
Size: 372 303
XhoI C'TCGA_G
Cuts at: 0 394 675
Size: 394 281
XmaI C'CCGG_G
Cuts at: 0 137 654 675
Size: 137 517 21
Fragments arranged by size:
517 137 21
XmnI GAAnn'nnTTC
Cuts at: 0 250 675
Size: 250 425
ZraI GAC'GTC
Cuts at: 0 152 675
Size: 152 523
Enzymes that do cut:
AatII AccI AhdI AlwNI ApoI AvaI BanI BanII
BciVI BfrBI BlpI Bme1580I BmgBI BplI BpmI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BssHII BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoRI HaeII Hin4I HincII MmeI MspA1I
NsiI PflMI PfoI PspXI PvuII SacI SmaI SmlI
SnaBI SrfI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BglI BglII BmrI BmtI Bpu10I BpuEI BsaBI BsaXI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI