Restriction map for
pFN21AE1996
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BbvCI BciVI BclI BfaI BfrI
BlnI BsaAI BsaBI BseAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MamI MfeI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 925 GAATTC [GA]^AATT[CT]
AflIII 1694 ACGCGT A^C[GA][CT]GT
AgeI 821 ACCGGT A^CCGGT
Aor51HI 1376 AGCGCT AGC^GCT
ApaI 237 GGGCCC GGGCC^C
ApoI 925 GAATTC [GA]^AATT[CT]
BcgI 1277 GCATGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1454 AGATCT A^GATCT
BsaI 1549 GAGACC GGTCTC(1/5)
BsaWI 821 ACCGGT [AT]^CCGG[AT]
BsiEI 1517 CGGCCG CG[GA][CT]^CG
BsiWI 935 CGTACG C^GTACG
BsmAI 1549 GAGAC GTCTC(1/5)
BsmI 371 GCATTC GAATGC(1/-1)
BspHI 662 TCATGA T^CATGA
BstAPI 84 GCAGCGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 1517 CGGCCG C^GGCCG
EclXI 1517 CGGCCG C^GGCCG
Eco47III 1376 AGCGCT AGC^GCT
Eco52I 1517 CGGCCG C^GGCCG
EcoRI 925 GAATTC G^AATTC
HincII 415 GTCAAC GT[CT]^[GA]AC
HindII 415 GTCAAC GT[CT]^[GA]AC
HinfI 409 GACTC G^A[ACGT]TC
MluI 1694 ACGCGT A^CGCGT
MslI 1533 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 556 GCCGGC GCC^GGC
NcoI 482 CCATGG C^CATGG
NgoMIV 556 GCCGGC G^CCGGC
PaeR7I 1237 CTCGAG C^TCGAG
PflMI 1317 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 821 ACCGGT A^CCGGT
PleI 409 GACTC GAGTC(4/5)
PvuII 916 CAGCTG CAG^CTG
RcaI 662 TCATGA T^CATGA
SfiI 1281 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 555 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1237 CTCGAG C^T[CT][GA]AG
StuI 509 AGGCCT AGG^CCT
Tsp509I 926 AATT ^AATT
TspRI 1258 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1317 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 482 CCATGGACGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1237 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbsI 1153 GAAGAC GAAGAC(2/6)
1396 GTCTTC
BglI 201 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1282 GCCATCGAGGC
BlpI 66 GCTCAGC GC^T[ACGT]AGC
1590 GCTCAGC
Bpu1102I 66 GCTCAGC GC^T[ACGT]AGC
1590 GCTCAGC
BpuAI 1153 GAAGAC GAAGAC(2/6)
1396 GTCTTC
BsgI 688 GTGCAG GTGCAG(16/14)
1096 GTGCAG
BspMI 844 ACCTGC ACCTGC(4/8)
992 ACCTGC
BsrBI 184 CCGCTC CCGCTC(-3/-3)
541 CCGCTC
CelII 66 GCTCAGC GC^T[ACGT]AGC
1590 GCTCAGC
EarI 544 CTCTTC CTCTTC(1/4)
340 GAAGAG
EcoNI 684 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
750 CCTGAAGGAGG
EcoT14I 482 CCATGG C^C[AT][AT]GG
506 CCAAGG
Ksp632I 544 CTCTTC CTCTTC(1/4)
340 GAAGAG
MaeIII 1078 GTCAC ^GT[ACGT]AC
1475 GTGAC
PpuMI 1367 AGGACCT [GA]G^G[AT]CC[CT]
1709 AGGACCT
SapI 340 GAAGAGC GCTCTTC(1/4)
543 GCTCTTC
ScaI 1181 AGTACT AGT^ACT
1241 AGTACT
SexAI 683 ACCTGGT A^CC[AT]GGT
1328 ACCTGGT
StyI 482 CCATGG C^C[AT][AT]GG
506 CCAAGG
Tsp45I 1078 GTCAC ^GT[GC]AC
1475 GTGAC
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PflFI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI TaqI
TseI Tth111I TthHB8I XhoII XmaCI
XmaI