(Circular) (Six-base) MAPSORT of: pFN21AE1996.seq Check: 2506 from: 1 to: 6430 pFN21AE1996 6430 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2858 2858 Size: 6430 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 2777 3372 3398 4493 5877 6145 1632 Size: 425 720 595 26 1095 1384 268 1917 Fragments arranged by size: 1917 1384 1095 720 595 425 268 26 AfeI AGC'GCT Cuts at: 3383 3383 Size: 6430 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 3699 5329 6306 1203 Size: 2496 1630 977 1327 Fragments arranged by size: 2496 1630 1327 977 AgeI A'CCGG_T Cuts at: 2826 5261 2826 Size: 2435 3995 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2039 3116 1184 Size: 855 1077 4498 Fragments arranged by size: 4498 1077 855 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 3566 3598 2042 Size: 32 1492 32 4874 Fragments arranged by size: 4874 1492 32 32 AlwNI CAG_nnn'CTG Cuts at: 3328 3376 3616 4173 5745 3328 Size: 48 240 557 1572 4013 Fragments arranged by size: 4013 1572 557 240 48 ApaI G_GGCC'C Cuts at: 2246 2246 Size: 6430 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 2930 3739 3965 4117 6226 1493 Size: 1437 809 226 152 2109 1697 Fragments arranged by size: 2109 1697 1437 809 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2129 2149 2168 2224 2234 2740 3242 Size: 73 186 20 19 56 10 506 502 Cuts at: 3242 3494 3755 1870 Size: 252 261 4545 Fragments arranged by size: 4545 506 502 261 252 186 73 56 20 19 10 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 2261 2300 2558 3221 Size: 325 217 627 338 136 39 258 663 Cuts at: 3221 3661 3677 3751 5206 618 Size: 440 16 74 1455 1842 Fragments arranged by size: 1842 1455 663 627 440 338 325 258 217 136 74 39 16 BanII G_rGCy'C Cuts at: 1391 2217 2246 2582 2675 2873 3243 3501 3570 Size: 826 29 336 93 198 370 258 69 Cuts at: 3570 3597 3749 1391 Size: 27 152 4072 Fragments arranged by size: 4072 826 370 336 258 198 152 93 69 29 27 BbeI G_GCGC'C Cuts at: 2129 2265 2562 3225 3665 3681 2129 Size: 136 297 663 440 16 4878 Fragments arranged by size: 4878 663 440 297 136 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3165 3394 928 Size: 971 1266 229 3964 Fragments arranged by size: 3964 1266 971 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3271 3305 4621 4655 1903 Size: 34 50 34 1250 34 1316 34 3678 Fragments arranged by size: 3678 1316 1250 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2212 3293 5041 Size: 107 122 71 631 605 540 1081 1748 Cuts at: 5041 136 Size: 1525 Fragments arranged by size: 1748 1525 1081 631 605 540 122 107 71 BglII A'GATC_T Cuts at: 3459 6425 3459 Size: 2966 3464 BlpI GC'TnA_GC Cuts at: 2072 3596 3847 2072 Size: 1524 251 4655 Fragments arranged by size: 4655 1524 251 Bme1580I G_kGCm'C Cuts at: 1790 2246 4491 5647 1790 Size: 456 2245 1156 2573 Fragments arranged by size: 2573 2245 1156 456 BmgBI CAC'GTC Cuts at: 1318 2725 1318 Size: 1407 5023 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3517 4161 5119 Size: 416 53 83 399 24 2100 644 958 Cuts at: 5119 442 Size: 1753 Fragments arranged by size: 2100 1753 958 644 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2794 3478 3682 2794 Size: 684 204 5542 Fragments arranged by size: 5542 684 204 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2254 2609 2918 3740 5090 1239 Size: 1015 355 309 822 1350 2579 Fragments arranged by size: 2579 1350 1015 822 355 309 Bpu10I CC'TnA_GC Cuts at: 3470 3470 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4555 5420 5718 4555 Size: 865 298 5267 Fragments arranged by size: 5267 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3548 5093 882 Size: 617 2049 1545 2219 Fragments arranged by size: 2219 2049 1545 617 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2126 2262 2404 2559 Size: 53 83 186 936 593 136 142 155 Cuts at: 2559 2765 3222 3662 3678 4237 4619 5254 275 Size: 206 457 440 16 559 382 635 1451 Fragments arranged by size: 1451 936 635 593 559 457 440 382 206 186 155 142 136 83 53 16 BsaWI w'CCGG_w Cuts at: 2826 4863 5261 5535 5682 6093 2826 Size: 2037 398 274 147 411 3163 Fragments arranged by size: 3163 2037 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3566 3596 1178 Size: 30 165 30 2163 30 4012 Fragments arranged by size: 4012 2163 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2881 2915 2918 3272 3542 3566 1186 Size: 1695 34 3 354 270 24 4050 Fragments arranged by size: 4050 1695 354 270 34 24 3 BseYI C'CCAG_C Cuts at: 2207 3673 5633 6129 2207 Size: 1466 1960 496 2508 Fragments arranged by size: 2508 1960 1466 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2714 3122 1163 Size: 573 978 408 4471 Fragments arranged by size: 4471 978 573 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3525 3880 4641 5669 1540 Size: 275 186 1524 355 761 1028 2301 Fragments arranged by size: 2301 1524 1028 761 355 275 186 BsiHKAI G_wGCw'C Cuts at: 2456 2873 3243 3501 3597 4491 4576 5647 2456 Size: 417 370 258 96 894 85 1071 3239 Fragments arranged by size: 3239 1071 894 417 370 258 96 85 BsiWI C'GTAC_G Cuts at: 2940 2940 Size: 6430 BsmI GAATG_Cn' Cuts at: 2376 3941 4034 2376 Size: 1565 93 4772 Fragments arranged by size: 4772 1565 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2246 2456 2582 2675 2873 3243 Size: 399 427 29 210 126 93 198 370 Cuts at: 3243 3501 3570 3597 3749 4491 4576 5647 1391 Size: 258 69 27 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 399 370 258 210 198 152 126 93 85 69 29 27 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 2667 4319 6049 2667 Size: 1652 1730 3048 Fragments arranged by size: 3048 1730 1652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2858 3006 3785 844 Size: 2014 148 779 3489 Fragments arranged by size: 3489 2014 779 148 BsrBI CCG'CTC Cuts at: 1394 1823 2191 2548 4317 1394 Size: 429 368 357 1769 3507 Fragments arranged by size: 3507 1769 429 368 357 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 2561 2826 5074 5261 1700 Size: 110 219 532 265 2248 187 2869 Fragments arranged by size: 2869 2248 532 265 219 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 2365 2474 3087 3443 4490 5502 2365 Size: 109 613 356 1047 1012 3293 Fragments arranged by size: 3293 1047 1012 613 356 109 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 794 Size: 1301 5129 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstYI r'GATC_y Cuts at: 1972 2309 2808 3066 3459 3760 4127 4512 4529 Size: 337 499 258 393 301 367 385 17 Cuts at: 4529 5981 6067 6079 6425 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 499 393 385 367 346 337 301 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2487 2793 3558 3603 3681 513 Size: 552 1422 306 765 45 78 3262 Fragments arranged by size: 3262 1422 765 552 306 78 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 3269 3946 4740 4760 3269 Size: 677 794 20 4939 Fragments arranged by size: 4939 794 677 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2508 2646 Size: 54 1425 50 275 18 531 147 138 Cuts at: 2646 3285 3393 3522 3546 3877 4766 6395 8 Size: 639 108 129 24 331 889 1629 43 Fragments arranged by size: 1629 1425 889 639 531 331 275 147 138 129 108 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3522 3877 1537 Size: 275 1710 355 4090 Fragments arranged by size: 4090 1710 355 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 2555 4360 1353 Size: 987 215 1805 3423 Fragments arranged by size: 3423 1805 987 215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2924 5017 5391 5537 387 Size: 1064 138 1335 2093 374 146 1280 Fragments arranged by size: 2093 1335 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2871 3241 3499 3595 2871 Size: 370 258 96 5706 Fragments arranged by size: 5706 370 258 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2254 2609 2777 2918 3372 3398 Size: 393 425 197 355 168 141 454 26 Cuts at: 3398 3740 4493 5090 5877 6145 1239 Size: 342 753 597 787 268 1524 Fragments arranged by size: 1524 787 753 597 454 425 393 355 342 268 197 168 141 26 EcoNI CCTnn'n_nnAGG Cuts at: 2693 2759 2693 Size: 66 6364 EcoO109I rG'GnC_Cy Cuts at: 2172 2243 3295 3373 3648 3715 2172 Size: 71 1052 78 275 67 4887 Fragments arranged by size: 4887 1052 275 78 71 67 EcoRI G'AATT_C Cuts at: 2930 3739 4117 2930 Size: 809 378 5243 Fragments arranged by size: 5243 809 378 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2129 2265 2562 2894 3225 3385 3665 3681 Size: 451 136 297 332 331 160 280 16 Cuts at: 3681 5577 1678 Size: 1896 2531 Fragments arranged by size: 2531 1896 451 332 331 297 280 160 136 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5071 5103 5145 5177 1373 Size: 32 135 32 3499 32 42 32 2626 Fragments arranged by size: 3499 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2422 3774 4020 669 Size: 988 281 484 1352 246 3079 Fragments arranged by size: 3079 1352 988 484 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KasI G'GCGC_C Cuts at: 2125 2261 2558 3221 3661 3677 2125 Size: 136 297 663 440 16 4878 Fragments arranged by size: 4878 663 440 297 136 16 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 3699 6306 3699 Size: 2607 3823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2405 5544 5728 1305 Size: 1100 3139 184 2007 Fragments arranged by size: 3139 2007 1100 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 3287 3395 10 Size: 54 1425 343 678 777 108 3045 Fragments arranged by size: 3045 1425 777 678 343 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 3542 4388 4747 4906 518 Size: 648 2376 846 359 159 2042 Fragments arranged by size: 2376 2042 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2211 2806 2923 3198 3486 3637 3673 Size: 486 119 595 117 275 288 151 36 Cuts at: 3673 3727 3846 4523 5671 5916 1606 Size: 54 119 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 595 486 288 275 245 151 119 119 117 54 36 NaeI GCC'GGC Cuts at: 2563 2563 Size: 6430 NarI GG'CG_CC Cuts at: 2126 2262 2559 3222 3662 3678 2126 Size: 136 297 663 440 16 4878 Fragments arranged by size: 4878 663 440 297 136 16 NcoI C'CATG_G Cuts at: 513 1065 2487 513 Size: 552 1422 4456 Fragments arranged by size: 4456 1422 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 2561 2561 Size: 6430 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3788 5333 1132 Size: 97 2559 1545 2229 Fragments arranged by size: 2559 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2257 3570 1348 Size: 909 1313 4208 Fragments arranged by size: 4208 1313 909 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 3328 1108 Size: 2220 4210 PfoI T'CCnGG_A Cuts at: 3306 3306 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3176 3208 3566 3598 4499 4531 6012 6044 3176 Size: 32 358 32 901 32 1481 32 3562 Fragments arranged by size: 3562 1481 901 358 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3373 3715 3373 Size: 342 6088 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2242 2242 Size: 6430 PspXI vC'TCGA_Gb Cuts at: 3242 3242 Size: 6430 PstI C_TGCA'G Cuts at: 830 2028 2103 2883 3372 3782 830 Size: 1198 75 780 489 410 3478 Fragments arranged by size: 3478 1198 780 489 410 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2923 2923 Size: 6430 SacI G_AGCT'C Cuts at: 2873 3243 3501 3597 2873 Size: 370 258 96 5706 Fragments arranged by size: 5706 370 258 96 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SapI GCTCTTCn'nnn_ Cuts at: 2340 2555 2340 Size: 215 6215 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 3188 3248 4678 6390 3188 Size: 60 1430 1712 3228 Fragments arranged by size: 3228 1712 1430 60 SexAI A'CCwGG_T Cuts at: 2688 3333 2688 Size: 645 5785 SfcI C'TryA_G Cuts at: 826 1045 2024 2099 2879 3368 3778 4913 5594 Size: 219 979 75 780 489 410 1135 681 Cuts at: 5594 5785 826 Size: 191 1471 Fragments arranged by size: 1471 1135 979 780 681 489 410 219 191 75 SfiI GGCCn_nnn'nGGCC Cuts at: 3293 3293 Size: 6430 SfoI GGC'GCC Cuts at: 2127 2263 2560 3223 3663 3679 2127 Size: 136 297 663 440 16 4878 Fragments arranged by size: 4878 663 440 297 136 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 2561 1700 Size: 861 5569 SmaI CCC'GGG Cuts at: 1872 2131 2151 2170 2226 2236 2742 3757 1872 Size: 259 20 19 56 10 506 1015 4545 Fragments arranged by size: 4545 1015 506 259 56 20 19 10 SmlI C'TyrA_G Cuts at: 820 1017 1943 3242 4534 5435 5697 820 Size: 197 926 1299 1292 901 262 1553 Fragments arranged by size: 1553 1299 1292 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SrfI GCCC'GGGC Cuts at: 2131 2131 Size: 6430 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 2516 1517 Size: 999 5431 StyI C'CwwG_G Cuts at: 513 1065 2487 2511 3869 513 Size: 552 1422 24 1358 3074 Fragments arranged by size: 3074 1422 1358 552 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2942 4457 4474 4627 4812 5257 325 Size: 1255 1362 1515 17 153 185 445 1498 Fragments arranged by size: 1515 1498 1362 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2064 3186 3246 Size: 274 80 33 51 1443 87 1122 60 Cuts at: 3246 4676 6388 96 Size: 1430 1712 138 Fragments arranged by size: 1712 1443 1430 1122 274 138 87 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6390 6422 6390 Size: 32 6398 Tth111I GACn'n_nGTC Cuts at: 1316 2417 2705 3134 3335 1316 Size: 1101 288 429 201 4411 Fragments arranged by size: 4411 1101 429 288 201 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2494 Size: 6430 XhoI C'TCGA_G Cuts at: 1943 3242 1943 Size: 1299 5131 XmaI C'CCGG_G Cuts at: 1870 2129 2149 2168 2224 2234 2740 3755 1870 Size: 259 20 19 56 10 506 1015 4545 Fragments arranged by size: 4545 1015 506 259 56 20 19 10 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SwaI