(Linear) (Six-base) MAPSORT of: KIEE1996.seq Check: 8070 from: 1 to: 1725 KIEE1996.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 853 1725 Size: 853 872 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 772 1367 1393 1725 Size: 52 720 595 26 332 Fragments arranged by size: 720 595 332 52 26 AfeI AGC'GCT Cuts at: 0 1378 1725 Size: 1378 347 AflIII A'CryG_T Cuts at: 0 1694 1725 Size: 1694 31 AgeI A'CCGG_T Cuts at: 0 821 1725 Size: 821 904 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 34 1111 1725 Size: 34 1077 614 Fragments arranged by size: 1077 614 34 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 37 69 1561 1593 1725 Size: 37 32 1492 32 132 Fragments arranged by size: 1492 132 37 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1323 1371 1611 1725 Size: 1323 48 240 114 Fragments arranged by size: 1323 240 114 48 ApaI G_GGCC'C Cuts at: 0 241 1725 Size: 241 1484 ApoI r'AATT_y Cuts at: 0 925 1725 Size: 925 800 AvaI C'yCGr_G Cuts at: 0 124 144 163 219 229 735 1237 1489 Size: 124 20 19 56 10 506 502 252 Cuts at: 1489 1725 Size: 236 Fragments arranged by size: 506 502 252 236 124 56 20 19 10 BanI G'GyrC_C Cuts at: 0 120 256 295 553 1216 1656 1672 1725 Size: 120 136 39 258 663 440 16 53 Fragments arranged by size: 663 440 258 136 120 53 39 16 BanII G_rGCy'C Cuts at: 0 212 241 577 670 868 1238 1496 1565 Size: 212 29 336 93 198 370 258 69 Cuts at: 1565 1592 1725 Size: 27 133 Fragments arranged by size: 370 336 258 212 198 133 93 69 29 27 BbeI G_GCGC'C Cuts at: 0 124 260 557 1220 1660 1676 1725 Size: 124 136 297 663 440 16 49 Fragments arranged by size: 663 440 297 136 124 49 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 1160 1389 1725 Size: 1160 229 336 Fragments arranged by size: 1160 336 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1266 1300 1725 Size: 1266 34 425 Fragments arranged by size: 1266 425 34 BglI GCCn_nnn'nGGC Cuts at: 0 207 1288 1725 Size: 207 1081 437 Fragments arranged by size: 1081 437 207 BglII A'GATC_T Cuts at: 0 1454 1725 Size: 1454 271 BlpI GC'TnA_GC Cuts at: 0 67 1591 1725 Size: 67 1524 134 Fragments arranged by size: 1524 134 67 Bme1580I G_kGCm'C Cuts at: 0 241 1725 Size: 241 1484 BmgBI CAC'GTC Cuts at: 0 720 1725 Size: 720 1005 BmrI ACTGGGnnnn_n' Cuts at: 0 1512 1725 Size: 1512 213 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 789 1473 1677 1725 Size: 789 684 204 48 Fragments arranged by size: 789 684 204 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 249 604 913 1725 Size: 249 355 309 812 Fragments arranged by size: 812 355 309 249 Bpu10I CC'TnA_GC Cuts at: 0 1465 1725 Size: 1465 260 BsaI GGTCTCn'nnnn_ Cuts at: 0 1543 1725 Size: 1543 182 BsaHI Gr'CG_yC Cuts at: 0 121 257 399 554 760 1217 1657 1673 Size: 121 136 142 155 206 457 440 16 Cuts at: 1673 1725 Size: 52 Fragments arranged by size: 457 440 206 155 142 136 121 52 16 BsaWI w'CCGG_w Cuts at: 0 821 1725 Size: 821 904 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1561 1591 1725 Size: 1561 30 134 Fragments arranged by size: 1561 134 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 876 910 913 1267 1537 1561 1725 Size: 876 34 3 354 270 24 164 Fragments arranged by size: 876 354 270 164 34 24 3 BseYI C'CCAG_C Cuts at: 0 202 1668 1725 Size: 202 1466 57 Fragments arranged by size: 1466 202 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 1117 1725 Size: 709 408 608 Fragments arranged by size: 709 608 408 BsiEI CG_ry'CG Cuts at: 0 1520 1725 Size: 1520 205 BsiHKAI G_wGCw'C Cuts at: 0 451 868 1238 1496 1592 1725 Size: 451 417 370 258 96 133 Fragments arranged by size: 451 417 370 258 133 96 BsiWI C'GTAC_G Cuts at: 0 935 1725 Size: 935 790 BsmI GAATG_Cn' Cuts at: 0 371 1725 Size: 371 1354 Bsp1286I G_dGCh'C Cuts at: 0 212 241 451 577 670 868 1238 1496 Size: 212 29 210 126 93 198 370 258 Cuts at: 1496 1565 1592 1725 Size: 69 27 133 Fragments arranged by size: 370 258 212 210 198 133 126 93 69 29 27 BspHI T'CATG_A Cuts at: 0 662 1725 Size: 662 1063 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 853 1001 1725 Size: 853 148 724 Fragments arranged by size: 853 724 148 BsrBI CCG'CTC Cuts at: 0 186 543 1725 Size: 186 357 1182 Fragments arranged by size: 1182 357 186 BsrFI r'CCGG_y Cuts at: 0 24 556 821 1725 Size: 24 532 265 904 Fragments arranged by size: 904 532 265 24 BssSI C'ACGA_G Cuts at: 0 360 469 1082 1438 1725 Size: 360 109 613 356 287 Fragments arranged by size: 613 360 356 287 109 BstAPI GCAn_nnn'nTGC Cuts at: 0 90 1725 Size: 90 1635 BstYI r'GATC_y Cuts at: 0 304 803 1061 1454 1725 Size: 304 499 258 393 271 Fragments arranged by size: 499 393 304 271 258 BtgI C'CryG_G Cuts at: 0 482 788 1553 1598 1676 1725 Size: 482 306 765 45 78 49 Fragments arranged by size: 765 482 306 78 49 45 BtsI GCAGTG_nn' Cuts at: 0 1264 1725 Size: 1264 461 EaeI y'GGCC_r Cuts at: 0 356 503 641 1280 1388 1517 1541 1725 Size: 356 147 138 639 108 129 24 184 Fragments arranged by size: 639 356 184 147 138 129 108 24 EagI C'GGCC_G Cuts at: 0 1517 1725 Size: 1517 208 EarI CTCTTCn'nnn_ Cuts at: 0 335 550 1725 Size: 335 215 1175 Fragments arranged by size: 1175 335 215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 919 1725 Size: 919 806 EcoICRI GAG'CTC Cuts at: 0 866 1236 1494 1590 1725 Size: 866 370 258 96 135 Fragments arranged by size: 866 370 258 135 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 249 604 772 913 1367 1393 1725 Size: 52 197 355 168 141 454 26 332 Fragments arranged by size: 454 355 332 197 168 141 52 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 688 754 1725 Size: 688 66 971 Fragments arranged by size: 971 688 66 EcoO109I rG'GnC_Cy Cuts at: 0 167 238 1290 1368 1643 1710 1725 Size: 167 71 1052 78 275 67 15 Fragments arranged by size: 1052 275 167 78 71 67 15 EcoRI G'AATT_C Cuts at: 0 925 1725 Size: 925 800 HaeII r_GCGC'y Cuts at: 0 124 260 557 889 1220 1380 1660 1676 Size: 124 136 297 332 331 160 280 16 Cuts at: 1676 1725 Size: 49 Fragments arranged by size: 332 331 297 280 160 136 124 49 16 HincII GTy'rAC Cuts at: 0 417 1725 Size: 417 1308 KasI G'GCGC_C Cuts at: 0 120 256 553 1216 1656 1672 1725 Size: 120 136 297 663 440 16 53 Fragments arranged by size: 663 440 297 136 120 53 16 MluI A'CGCG_T Cuts at: 0 1694 1725 Size: 1694 31 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 400 1725 Size: 400 1325 MscI TGG'CCA Cuts at: 0 505 1282 1390 1725 Size: 505 777 108 335 Fragments arranged by size: 777 505 335 108 MslI CAynn'nnrTG Cuts at: 0 1537 1725 Size: 1537 188 MspA1I CmG'CkG Cuts at: 0 87 206 801 918 1193 1481 1632 1668 Size: 87 119 595 117 275 288 151 36 Cuts at: 1668 1722 1725 Size: 54 3 Fragments arranged by size: 595 288 275 151 119 117 87 54 36 3 NaeI GCC'GGC Cuts at: 0 558 1725 Size: 558 1167 NarI GG'CG_CC Cuts at: 0 121 257 554 1217 1657 1673 1725 Size: 121 136 297 663 440 16 52 Fragments arranged by size: 663 440 297 136 121 52 16 NcoI C'CATG_G Cuts at: 0 482 1725 Size: 482 1243 NgoMIV G'CCGG_C Cuts at: 0 556 1725 Size: 556 1169 PasI CC'CwG_GG Cuts at: 0 252 1565 1725 Size: 252 1313 160 Fragments arranged by size: 1313 252 160 PflMI CCAn_nnn'nTGG Cuts at: 0 1323 1725 Size: 1323 402 PfoI T'CCnGG_A Cuts at: 0 1301 1725 Size: 1301 424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1171 1203 1561 1593 1725 Size: 1171 32 358 32 132 Fragments arranged by size: 1171 358 132 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1368 1710 1725 Size: 1368 342 15 Fragments arranged by size: 1368 342 15 PspOMI G'GGCC_C Cuts at: 0 237 1725 Size: 237 1488 PspXI vC'TCGA_Gb Cuts at: 0 1237 1725 Size: 1237 488 PstI C_TGCA'G Cuts at: 0 23 98 878 1367 1725 Size: 23 75 780 489 358 Fragments arranged by size: 780 489 358 75 23 PvuII CAG'CTG Cuts at: 0 918 1725 Size: 918 807 SacI G_AGCT'C Cuts at: 0 868 1238 1496 1592 1725 Size: 868 370 258 96 133 Fragments arranged by size: 868 370 258 133 96 SapI GCTCTTCn'nnn_ Cuts at: 0 335 550 1725 Size: 335 215 1175 Fragments arranged by size: 1175 335 215 ScaI AGT'ACT Cuts at: 0 1183 1243 1725 Size: 1183 60 482 Fragments arranged by size: 1183 482 60 SexAI A'CCwGG_T Cuts at: 0 683 1328 1725 Size: 683 645 397 Fragments arranged by size: 683 645 397 SfcI C'TryA_G Cuts at: 0 19 94 874 1363 1725 Size: 19 75 780 489 362 Fragments arranged by size: 780 489 362 75 19 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1288 1725 Size: 1288 437 SfoI GGC'GCC Cuts at: 0 122 258 555 1218 1658 1674 1725 Size: 122 136 297 663 440 16 51 Fragments arranged by size: 663 440 297 136 122 51 16 SgrAI Cr'CCGG_yG Cuts at: 0 556 1725 Size: 556 1169 SmaI CCC'GGG Cuts at: 0 126 146 165 221 231 737 1725 Size: 126 20 19 56 10 506 988 Fragments arranged by size: 988 506 126 56 20 19 10 SmlI C'TyrA_G Cuts at: 0 1237 1725 Size: 1237 488 SrfI GCCC'GGGC Cuts at: 0 126 1725 Size: 126 1599 StuI AGG'CCT Cuts at: 0 511 1725 Size: 511 1214 StyI C'CwwG_G Cuts at: 0 482 506 1725 Size: 482 24 1219 Fragments arranged by size: 1219 482 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 937 1725 Size: 937 788 TatI w'GTAC_w Cuts at: 0 59 1181 1241 1725 Size: 59 1122 60 484 Fragments arranged by size: 1122 484 60 59 Tth111I GACn'n_nGTC Cuts at: 0 412 700 1129 1330 1725 Size: 412 288 429 201 395 Fragments arranged by size: 429 412 395 288 201 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 489 1725 Size: 489 1236 XhoI C'TCGA_G Cuts at: 0 1237 1725 Size: 1237 488 XmaI C'CCGG_G Cuts at: 0 124 144 163 219 229 735 1725 Size: 124 20 19 56 10 506 990 Fragments arranged by size: 990 506 124 56 20 19 10 Enzymes that do cut: AarI AcuI AfeI AflIII AgeI AlfI AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII HincII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SapI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmtI BpuEI BsaAI BsaBI BsmBI BspEI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SbfI SgfI SnaBI SpeI SphI SspI SwaI TstI XbaI XmnI ZraI