Restriction map for
pFN21AE3529
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HinfI HpaI HpaII KasI KspI
MaeI MamI MfeI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 490 GGTACC G^GTACC
AccI 345 GTATAC GT^[AC][GT]AC
AlwI 375 GATCC GGATC(4/5)
AlwNI 145 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 504 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 490 GGTACC G^GTACC
AvaI 586 CTCGAG C^[CT]CG[GA]G
BalI 392 TGGCCA TGG^CCA
BanI 490 GGTACC G^G[CT][GA]CC
BanII 583 GAGCTC G[GA]GC[CT]^C
BbsI 7 GAAGAC GAAGAC(2/6)
BcgI 641 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 7 GAAGAC GAAGAC(2/6)
BsaAI 156 CACGTA [CT]AC^GT[GA]
BsaI 652 GAGACC GGTCTC(1/5)
BsiWI 158 CGTACG C^GTACG
BsiYI 657 CCCATCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 657 CCCATCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 474 CGTCTC CGTCTC(1/5)
BsmFI 224 GGGAC GGGAC(10/14)
BsoBI 586 CTCGAG C^[CT]CG[GA]G
BsrBI 241 GAGCGG CCGCTC(-3/-3)
Bst1107I 345 GTATAC GTA^TAC
BstYI 374 AGATCC [GA]^GATC[CT]
BstZ17I 345 GTATAC GTA^TAC
CfoI 89 GCGC GCG^C
DdeI 334 CTGAG C^T[ACGT]AG
DsaI 188 CCACGG C^C[GA][CT]GG
EaeI 392 TGGCCA [CT]^GGCC[GA]
EcoT14I 36 CCAAGG C^C[AT][AT]GG
HhaI 89 GCGC GCG^C
HinP1I 89 GCGC G^CGC
HindIII 432 AAGCTT A^AGCTT
HphI 320 TCACC GGTGA(8/7)
KpnI 490 GGTACC GGTAC^C
MflI 374 AGATCC [GA]^GATC[CT]
MluNI 392 TGGCCA TGG^CCA
MscI 392 TGGCCA TGG^CCA
MspA1I 148 CAGCTG C[AC]G^C[GT]G
PaeR7I 586 CTCGAG C^TCGAG
PvuII 148 CAGCTG CAG^CTG
SacI 583 GAGCTC GAGCT^C
SapI 507 GCTCTTC GCTCTTC(1/4)
SexAI 340 ACCTGGT A^CC[AT]GGT
SfcI 324 CTATAG C^T[GA][CT]AG
SmlI 586 CTCGAG C^T[CT][GA]AG
StyI 36 CCAAGG C^C[AT][AT]GG
TspRI 92 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 586 CTCGAG C^TCGAG
XhoII 374 AGATCC [GA]^GATC[CT]
XmnI 504 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 243 GCGG CCGC(-3/-1)
670 GCGG
AspHI 336 GAGCAC G[AT]GC[AT]^C
583 GAGCTC
AvaII 33 GGACC G^G[AT]CC
203 GGACC
BmyI 336 GAGCAC G[AGT]GC[ACT]^C
583 GAGCTC
BseRI 613 GAGGAG GAGGAG(10/8)
664 GAGGAG
BsiHKAI 336 GAGCAC G[AT]GC[AT]^C
583 GAGCTC
Bsp1286I 336 GAGCAC G[AGT]GC[ACT]^C
583 GAGCTC
Bsp1407I 173 TGTACA T^GTACA
306 TGTACA
BsrGI 173 TGTACA T^GTACA
306 TGTACA
BstXI 104 CCAAACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
206 CCATCCTTGTGG
DpnII 69 GATC ^GATC
375 GATC
EarI 508 CTCTTC CTCTTC(1/4)
610 GAAGAG
EcoT22I 256 ATGCAT ATGCA^T
381 ATGCAT
Ksp632I 508 CTCTTC CTCTTC(1/4)
610 GAAGAG
MaeIII 319 GTCAC ^GT[ACGT]AC
421 GTGAC
MboI 69 GATC ^GATC
375 GATC
MwoI 89 GCGCAGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
141 GCCACAGCAGC
NdeII 69 GATC ^GATC
375 GATC
NsiI 256 ATGCAT ATGCA^T
381 ATGCAT
NspI 254 GCATGC [GA]CATG^[CT]
258 GCATGC
Sau3AI 69 GATC ^GATC
375 GATC
SphI 254 GCATGC GCATG^C
258 GCATGC
SspBI 173 TGTACA T^GTACA
306 TGTACA
Tsp45I 319 GTCAC ^GT[GC]AC
421 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsmAI
BssKI BstF5I BstNI Cac8I Cfr13I
EcoRII Fnu4HI FokI HaeIII ItaI
MaeII MboII MnlI MslI MvaI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TthHB8I