(Circular) (Six-base) MAPSORT of: pFN21AE3529.seq Check: 9277 from: 1 to: 5392 pFN21AE3529 5392 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3202 278 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2351 2735 1937 Size: 414 384 4594 Fragments arranged by size: 4594 414 384 Acc65I G'GTAC_C Cuts at: 2495 2713 2495 Size: 218 5174 AclI AA'CG_TT Cuts at: 1563 3519 3892 1563 Size: 1956 373 3063 Fragments arranged by size: 3063 1956 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2584 3455 4839 5107 1632 Size: 952 871 1384 268 1917 Fragments arranged by size: 1917 1384 952 871 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5195 AflIII A'CryG_T Cuts at: 1203 4291 5268 1203 Size: 3088 977 1327 Fragments arranged by size: 3088 1327 977 AgeI A'CCGG_T Cuts at: 4223 4223 Size: 5392 AhdI GACnn_n'nnGTC Cuts at: 4121 4121 Size: 5392 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5392 AlwNI CAG_nnn'CTG Cuts at: 2155 3135 4707 2155 Size: 980 1572 2840 Fragments arranged by size: 2840 1572 980 ApaLI G'TGCA_C Cuts at: 3449 4605 3449 Size: 1156 4236 ApoI r'AATT_y Cuts at: 1493 2701 2927 3079 5188 1493 Size: 1208 226 152 2109 1697 Fragments arranged by size: 2109 1697 1208 226 152 AseI AT'TA_AT Cuts at: 160 1032 3946 160 Size: 872 2914 1606 Fragments arranged by size: 2914 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 AvaI C'yCGr_G Cuts at: 1870 1943 2591 2717 1870 Size: 73 648 126 4545 Fragments arranged by size: 4545 648 126 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2487 2520 2487 Size: 33 5359 BamHI G'GATC_C Cuts at: 2722 3089 2722 Size: 367 5025 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 2713 4168 618 Size: 325 217 627 708 218 1455 1842 Fragments arranged by size: 1842 1455 708 627 325 218 217 BanII G_rGCy'C Cuts at: 1391 2592 2711 1391 Size: 1201 119 4072 Fragments arranged by size: 4072 1201 119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 928 Size: 971 120 4301 Fragments arranged by size: 4301 971 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2635 2669 3583 3617 1903 Size: 34 698 34 914 34 3678 Fragments arranged by size: 3678 914 698 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3284 4494 3284 Size: 1210 4182 BclI T'GATC_A Cuts at: 1548 1548 Size: 5392 BfrBI ATG'CAT Cuts at: 2263 2388 2263 Size: 125 5267 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4003 136 Size: 107 122 71 631 605 2331 1525 Fragments arranged by size: 2331 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5387 5387 Size: 5392 BlpI GC'TnA_GC Cuts at: 2809 2809 Size: 5392 Bme1580I G_kGCm'C Cuts at: 1790 3453 4609 1790 Size: 1663 1156 2573 Fragments arranged by size: 2573 1663 1156 BmgBI CAC'GTC Cuts at: 1318 2626 1318 Size: 1308 4084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3123 4081 442 Size: 416 53 83 399 24 1706 958 1753 Fragments arranged by size: 1753 1706 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5392 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2695 2695 Size: 5392 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4052 1239 Size: 2813 2579 Bpu10I CC'TnA_GC Cuts at: 2339 2339 Size: 5392 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3517 4382 4680 3517 Size: 865 298 4229 Fragments arranged by size: 4229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2651 4055 882 Size: 617 1152 1404 2219 Fragments arranged by size: 2219 1404 1152 617 BsaAI yAC'GTr Cuts at: 493 1204 2163 3183 493 Size: 711 959 1020 2702 Fragments arranged by size: 2702 1020 959 711 BsaBI GATnn'nnATC Cuts at: 5386 5386 Size: 5392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3199 3581 4216 275 Size: 53 83 186 936 1666 382 635 1451 Fragments arranged by size: 1666 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3825 4223 4497 4644 5055 3825 Size: 398 274 147 411 4162 Fragments arranged by size: 4162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5167 Fragments arranged by size: 5167 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2633 2684 1186 Size: 1447 51 3894 Fragments arranged by size: 3894 1447 51 BseYI C'CCAG_C Cuts at: 4595 5091 4595 Size: 496 4896 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4819 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2842 3603 4631 1540 Size: 275 186 841 761 1028 2301 Fragments arranged by size: 2301 1028 841 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2345 2592 3453 3538 4609 2345 Size: 247 861 85 1071 3128 Fragments arranged by size: 3128 1071 861 247 85 BsiWI C'GTAC_G Cuts at: 2163 2163 Size: 5392 BsmI GAATG_Cn' Cuts at: 2903 2996 2903 Size: 93 5299 BsmBI CGTCTCn'nnnn_ Cuts at: 2485 2485 Size: 5392 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2345 2592 2711 3453 3538 4609 1391 Size: 399 555 247 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 555 399 247 119 85 BspEI T'CCGG_A Cuts at: 5055 5055 Size: 5392 BspHI T'CATG_A Cuts at: 3281 5011 3281 Size: 1730 3662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 844 Size: 1903 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2248 3279 1394 Size: 429 425 1031 3507 Fragments arranged by size: 3507 1031 429 425 BsrDI GCAATG_nn' Cuts at: 65 1248 3887 4061 65 Size: 1183 2639 174 1396 Fragments arranged by size: 2639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4036 4223 1700 Size: 110 2226 187 2869 Fragments arranged by size: 2869 2226 187 110 BsrGI T'GTAC_A Cuts at: 96 2178 2311 96 Size: 2082 133 3177 Fragments arranged by size: 3177 2082 133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5392 BssSI C'ACGA_G Cuts at: 3452 4464 3452 Size: 1012 4380 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5392 BstBI TT'CG_AA Cuts at: 4196 4196 Size: 5392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5392 BstXI CCAn_nnnn'nTGG Cuts at: 2116 2218 2116 Size: 102 5290 BstYI r'GATC_y Cuts at: 1972 2379 2722 3089 3474 3491 4943 5029 5041 Size: 407 343 367 385 17 1452 86 12 Cuts at: 5041 5387 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 407 385 367 346 343 86 17 12 BstZ17I GTA'TAC Cuts at: 2352 2352 Size: 5392 BtgI C'CryG_G Cuts at: 513 1065 2004 2193 513 Size: 552 939 189 3712 Fragments arranged by size: 3712 939 552 189 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4653 Fragments arranged by size: 4653 683 56 BtsI GCAGTG_nn' Cuts at: 2103 2908 3702 3722 2103 Size: 805 794 20 3773 Fragments arranged by size: 3773 805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5357 DraI TTT'AAA Cuts at: 2696 2696 Size: 5392 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4399 809 Size: 507 3083 1802 Fragments arranged by size: 3083 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2839 3728 Size: 54 1425 50 275 18 567 442 889 Cuts at: 3728 5357 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 567 442 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2839 1537 Size: 275 1027 4090 Fragments arranged by size: 4090 1027 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2519 2610 3322 1353 Size: 1166 91 712 3423 Fragments arranged by size: 3423 1166 712 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3979 4353 4499 387 Size: 1064 138 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2590 2590 Size: 5392 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2584 3455 4052 4839 5107 1239 Size: 393 952 871 597 787 268 1524 Fragments arranged by size: 1524 952 871 787 597 393 268 EcoRI G'AATT_C Cuts at: 2701 3079 2701 Size: 378 5014 FspI TGC'GCA Cuts at: 3898 3898 Size: 5392 HaeII r_GCGC'y Cuts at: 1678 4539 1678 Size: 2861 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 4033 4065 4107 Size: 32 135 32 1076 32 1353 32 42 Cuts at: 4107 4139 1373 Size: 32 2626 Fragments arranged by size: 2626 1353 1076 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 2982 669 Size: 988 281 798 246 3079 Fragments arranged by size: 3079 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 2437 748 Size: 1689 3703 HpaI GTT'AAC Cuts at: 2982 2982 Size: 5392 KpnI G_GTAC'C Cuts at: 2499 2717 2499 Size: 218 5174 MfeI C'AATT_G Cuts at: 2991 2991 Size: 5392 MluI A'CGCG_T Cuts at: 5268 5268 Size: 5392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 4506 4690 1305 Size: 711 2490 184 2007 Fragments arranged by size: 2490 2007 711 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 10 Size: 54 1425 343 567 3003 Fragments arranged by size: 3003 1425 567 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2300 2393 2423 3350 3709 3868 Size: 648 1050 84 93 30 927 359 159 Cuts at: 3868 518 Size: 2042 Fragments arranged by size: 2042 1050 927 648 359 159 93 84 30 MspA1I CmG'CkG Cuts at: 1606 2155 2808 3485 4633 4878 1606 Size: 549 653 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 653 549 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3901 Fragments arranged by size: 3901 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5392 NotI GC'GGCC_GC Cuts at: 2839 2839 Size: 5392 NsiI A_TGCA'T Cuts at: 2265 2390 2265 Size: 125 5267 NspI r_CATG'y Cuts at: 1132 1229 2263 2267 2750 4295 1132 Size: 97 1034 4 483 1545 2229 Fragments arranged by size: 2229 1545 1034 483 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5392 PciI A'CATG_T Cuts at: 4291 4291 Size: 5392 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5392 PmeI GTTT'AAAC Cuts at: 2696 2696 Size: 5392 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3461 3493 4974 5006 3461 Size: 32 1481 32 3847 Fragments arranged by size: 3847 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5392 PsiI TTA'TAA Cuts at: 2962 2962 Size: 5392 PstI C_TGCA'G Cuts at: 830 2744 830 Size: 1914 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5392 PvuII CAG'CTG Cuts at: 2155 2155 Size: 5392 SacI G_AGCT'C Cuts at: 2592 2592 Size: 5392 SalI G'TCGA_C Cuts at: 1936 2734 1936 Size: 798 4594 SapI GCTCTTCn'nnn_ Cuts at: 2519 2519 Size: 5392 SbfI CC_TGCA'GG Cuts at: 2744 2744 Size: 5392 ScaI AGT'ACT Cuts at: 3640 5352 3640 Size: 1712 3680 SexAI A'CCwGG_T Cuts at: 2345 2345 Size: 5392 SfcI C'TryA_G Cuts at: 826 1045 2329 2740 3875 4556 4747 826 Size: 219 1284 411 1135 681 191 1471 Fragments arranged by size: 1471 1284 1135 681 411 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5392 SmaI CCC'GGG Cuts at: 1872 2719 1872 Size: 847 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 3496 4397 4659 820 Size: 197 926 648 905 901 262 1553 Fragments arranged by size: 1553 926 905 901 648 262 197 SnaBI TAC'GTA Cuts at: 493 3183 493 Size: 2690 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5392 SphI G_CATG'C Cuts at: 1132 2263 2267 2750 1132 Size: 1131 4 483 3774 Fragments arranged by size: 3774 1131 483 4 SspI AAT'ATT Cuts at: 5 52 3316 5 Size: 47 3264 2081 Fragments arranged by size: 3264 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5392 StyI C'CwwG_G Cuts at: 513 1065 2004 2041 2831 513 Size: 552 939 37 790 3074 Fragments arranged by size: 3074 939 790 552 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3419 3436 3589 3774 4219 325 Size: 1255 1839 17 153 185 445 1498 Fragments arranged by size: 1839 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 2311 3638 Size: 274 80 33 51 1443 201 133 1327 Cuts at: 3638 5350 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1327 274 201 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5352 5384 5352 Size: 32 5360 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5392 XbaI T'CTAG_A Cuts at: 2728 2728 Size: 5392 XhoI C'TCGA_G Cuts at: 1943 2591 1943 Size: 648 4744 XmaI C'CCGG_G Cuts at: 1870 2717 1870 Size: 847 4545 XmnI GAAnn'nnTTC Cuts at: 2513 3521 2513 Size: 1008 4384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3200 276 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI