Restriction map for
pFN21AE3529
(Linear) (Six-base) MAPSORT of: KIEE3529.seq Check: 8198 from: 1 to: 687
KIEE3529.orf
With 182 enzymes: SgfI *
July 3, 2012 18:06 ..
AccI GT'mk_AC
Cuts at: 0 346 687
Size: 346 341
Acc65I G'GTAC_C
Cuts at: 0 490 687
Size: 490 197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 687
Size: 579 108
AlwNI CAG_nnn'CTG
Cuts at: 0 150 687
Size: 150 537
AvaI C'yCGr_G
Cuts at: 0 586 687
Size: 586 101
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 482 515 687
Size: 482 33 172
Fragments arranged by size:
482 172 33
BanI G'GyrC_C
Cuts at: 0 490 687
Size: 490 197
BanII G_rGCy'C
Cuts at: 0 587 687
Size: 587 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 687
Size: 14 673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 630 664 687
Size: 630 34 23
Fragments arranged by size:
630 34 23
BfrBI ATG'CAT
Cuts at: 0 258 383 687
Size: 258 125 304
Fragments arranged by size:
304 258 125
BmgBI CAC'GTC
Cuts at: 0 621 687
Size: 621 66
Bpu10I CC'TnA_GC
Cuts at: 0 334 687
Size: 334 353
BsaI GGTCTCn'nnnn_
Cuts at: 0 646 687
Size: 646 41
BsaAI yAC'GTr
Cuts at: 0 158 687
Size: 158 529
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 628 679 687
Size: 628 51 8
Fragments arranged by size:
628 51 8
BsiHKAI G_wGCw'C
Cuts at: 0 340 587 687
Size: 340 247 100
Fragments arranged by size:
340 247 100
BsiWI C'GTAC_G
Cuts at: 0 158 687
Size: 158 529
BsmBI CGTCTCn'nnnn_
Cuts at: 0 480 687
Size: 480 207
Bsp1286I G_dGCh'C
Cuts at: 0 340 587 687
Size: 340 247 100
Fragments arranged by size:
340 247 100
BsrBI CCG'CTC
Cuts at: 0 243 687
Size: 243 444
BsrGI T'GTAC_A
Cuts at: 0 173 306 687
Size: 173 133 381
Fragments arranged by size:
381 173 133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 213 687
Size: 111 102 474
Fragments arranged by size:
474 111 102
BstYI r'GATC_y
Cuts at: 0 374 687
Size: 374 313
BstZ17I GTA'TAC
Cuts at: 0 347 687
Size: 347 340
BtgI C'CryG_G
Cuts at: 0 188 687
Size: 188 499
BtsI GCAGTG_nn'
Cuts at: 0 98 687
Size: 98 589
EaeI y'GGCC_r
Cuts at: 0 392 687
Size: 392 295
EarI CTCTTCn'nnn_
Cuts at: 0 514 605 687
Size: 514 91 82
Fragments arranged by size:
514 91 82
EcoICRI GAG'CTC
Cuts at: 0 585 687
Size: 585 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 687
Size: 579 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 687
Size: 643 32 12
Fragments arranged by size:
643 32 12
HindIII A'AGCT_T
Cuts at: 0 432 687
Size: 432 255
KpnI G_GTAC'C
Cuts at: 0 494 687
Size: 494 193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 687
Size: 11 676
MscI TGG'CCA
Cuts at: 0 394 687
Size: 394 293
MslI CAynn'nnrTG
Cuts at: 0 211 295 388 418 687
Size: 211 84 93 30 269
Fragments arranged by size:
269 211 93 84 30
MspA1I CmG'CkG
Cuts at: 0 150 687
Size: 150 537
NsiI A_TGCA'T
Cuts at: 0 260 385 687
Size: 260 125 302
Fragments arranged by size:
302 260 125
NspI r_CATG'y
Cuts at: 0 258 262 687
Size: 258 4 425
Fragments arranged by size:
425 258 4
PvuII CAG'CTG
Cuts at: 0 150 687
Size: 150 537
SacI G_AGCT'C
Cuts at: 0 587 687
Size: 587 100
SapI GCTCTTCn'nnn_
Cuts at: 0 514 687
Size: 514 173
SexAI A'CCwGG_T
Cuts at: 0 340 687
Size: 340 347
SfcI C'TryA_G
Cuts at: 0 324 687
Size: 324 363
SmlI C'TyrA_G
Cuts at: 0 586 687
Size: 586 101
SphI G_CATG'C
Cuts at: 0 258 262 687
Size: 258 4 425
Fragments arranged by size:
425 258 4
StyI C'CwwG_G
Cuts at: 0 36 687
Size: 36 651
TatI w'GTAC_w
Cuts at: 0 173 306 687
Size: 173 133 381
Fragments arranged by size:
381 173 133
XhoI C'TCGA_G
Cuts at: 0 586 687
Size: 586 101
XmnI GAAnn'nnTTC
Cuts at: 0 508 687
Size: 508 179
Enzymes that do cut:
AccI Acc65I AcuI AlwNI AvaI BaeI BanI BanII
BbsI BcgI BfrBI BmgBI Bpu10I BsaI BsaAI BseRI
BsiHKAI BsiWI BsmBI Bsp1286I BsrBI BsrGI BstXI BstYI
BstZ17I BtgI BtsI EaeI EarI EcoICRI Eco57MI Hin4I
HindIII KpnI MmeI MscI MslI MspA1I NsiI NspI
PvuII SacI SapI SexAI SfcI SmlI SphI StyI
TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsmI
BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XmaI
ZraI