################################## # NO CUT ENZYMES ( 39 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AscI Asp718I AspEI AviII AvrII BbvCI BglII BlnI BsiWI BstBI CpoI Eam1105I EcoNI EcoRV FspI HpaI KpnI MluI NheI NruI NspV PacI PinAI PmeI Psp1406I RsrII SacI SfiI SfuI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 4097 TCCGGA T^CCGGA AflII 4690 CTTAAG C^TTAAG AlwNI 225 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 3066 AGCGCT AGC^GCT BamHI 3326 GGATCC G^GATCC BcgI 3243 GCAGGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4690 CTTAAG C^TTAAG BsaI 4594 GAGACC GGTCTC(1/5) BsaWI 4097 TCCGGA [AT]^CCGG[AT] BseAI 4097 TCCGGA T^CCGGA Bsp1407I 3038 TGTACA T^GTACA BspDI 2212 ATCGAT AT^CGAT BspEI 4097 TCCGGA T^CCGGA BsrGI 3038 TGTACA T^GTACA BssSI 5046 CACGAG CACGAG(-5/-1) Bst1107I 6304 GTATAC GTA^TAC BstEII 6319 GGTTACC G^GT[ACGT]ACC BstPI 6319 GGTTACC G^GT[ACGT]ACC BstZ17I 6304 GTATAC GTA^TAC ClaI 2212 ATCGAT AT^CGAT DraI 6012 TTTAAA TTT^AAA Eco47III 3066 AGCGCT AGC^GCT EcoO65I 6319 GGTTACC G^GT[ACGT]ACC EcoRI 2302 GAATTC G^AATTC EcoT22I 4993 ATGCAT ATGCA^T FseI 311 GGCCGGCC GGCCGG^CC MfeI 5712 CAATTG C^AATTG MroI 4097 TCCGGA T^CCGGA MunI 5712 CAATTG C^AATTG NaeI 312 GCCGGC GCC^GGC NgoMIV 312 GCCGGC G^CCGGC NsiI 4993 ATGCAT ATGCA^T PaeR7I 844 CTCGAG C^TCGAG PflMI 3306 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1641 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 3913 CGATCG CGAT^CG SalI 337 GTCGAC G^TCGAC SexAI 6323 ACCTGGT A^CC[AT]GGT SspBI 3038 TGTACA T^GTACA StuI 4 AGGCCT AGG^CCT Van91I 3306 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1856 CCATCCTGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 844 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Alw44I 3388 GTGCAC G^TGCAC 4474 GTGCAC ApaI 10 GGGCCC GGGCC^C 1236 GGGCCC ApaLI 3388 GTGCAC G^TGCAC 4474 GTGCAC AseI 1702 ATTAAT AT^TAAT 4326 ATTAAT AsnI 1702 ATTAAT AT^TAAT 4326 ATTAAT Asp700I 5876 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6054 GAAACATTTC BanII 10 GGGCCC G[GA]GC[CT]^C 1236 GGGCCC BbrPI 1279 CACGTG CAC^GTG 1345 CACGTG BciVI 5015 GGATAC GTATCC(6/5) 3229 GTATCC BglI 457 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6241 GCCATCCAGGC BsmBI 99 CGTCTC CGTCTC(1/5) 483 CGTCTC BsrBI 14 CCGCTC CCGCTC(-3/-3) 213 GAGCGG BsrFI 72 GCCGGT [GA]^CCGG[CT] 312 GCCGGC BstXI 3062 CCACAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3077 CCACCTTCCTGG Cfr10I 72 GCCGGT [GA]^CCGG[CT] 312 GCCGGC DraIII 540 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 3469 CACTATGTG DrdI 274 GACTTGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5023 GACCAGATCGTC HindIII 1568 AAGCTT A^AGCTT 5879 AAGCTT NcoI 240 CCATGG C^CATGG 1674 CCATGG NdeI 3981 CATATG CA^TATG 6521 CATATG PmaCI 1279 CACGTG CAC^GTG 1345 CACGTG PmlI 1279 CACGTG CAC^GTG 1345 CACGTG PshBI 1702 ATTAAT AT^TAAT 4326 ATTAAT SapI 5114 GAAGAGC GCTCTTC(1/4) 2619 GCTCTTC SbfI 1005 CCTGCAGG CCTGCA^GG 2113 CCTGCAGG ScaI 770 AGTACT AGT^ACT 2419 AGTACT SmaI 135 CCCGGG CCC^GGG 406 CCCGGG Sse8387I 1005 CCTGCAGG CCTGCA^GG 2113 CCTGCAGG SspI 2446 AATATT AAT^ATT 5085 AATATT XmaCI 135 CCCGGG C^CCGGG 406 CCCGGG XmaI 135 CCCGGG C^CCGGG 406 CCCGGG XmnI 5876 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6054 GAAACATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 144 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AocI ApoI AspHI AspI AvaI AvaII BalI BanI BbeI BbsI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NotI NspI PflFI PleI PpuMI PstI PvuII RcaI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII