Restriction map for
pFN21AE5847
(Circular) (Six-base) MAPSORT of: pFN21AE5847.seq Check: 3927 from: 1 to: 11464
pFN21AE5847 11464 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2773 5472 2773
Size: 2699 8765
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9274 278
Size: 53 83 186 936 7738 2468
Fragments arranged by size:
7738 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 4088 7423 8310 8807 1937
Size: 406 1745 3335 887 497 4594
Fragments arranged by size:
4594 3335 1745 887 497 406
Acc65I G'GTAC_C
Cuts at: 8785 8785
Size: 11464
AclI AA'CG_TT
Cuts at: 1563 9591 9964 1563
Size: 8028 373 3063
Fragments arranged by size:
8028 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3280 3483 3640 4612 6895 7872 8738 9527
Size: 1648 203 157 972 2283 977 866 789
Cuts at: 9527 10911 11179 1632
Size: 1384 268 1917
Fragments arranged by size:
2283 1917 1648 1384 977 972 866 789
268 203 157
AfeI AGC'GCT
Cuts at: 5073 5073
Size: 11464
AflII C'TTAA_G
Cuts at: 820 1017 6695 820
Size: 197 5678 5589
Fragments arranged by size:
5678 5589 197
AflIII A'CryG_T
Cuts at: 1203 3285 3386 4584 5322 6114 10363 11340 1203
Size: 2082 101 1198 738 792 4249 977 1327
Fragments arranged by size:
4249 2082 1327 1198 977 792 738 101
AgeI A'CCGG_T
Cuts at: 10295 10295
Size: 11464
AhdI GACnn_n'nnGTC
Cuts at: 10193 10193
Size: 11464
AleI CACnn'nnGTG
Cuts at: 1166 4778 1166
Size: 3612 7852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5480 7865 1184
Size: 4296 2385 4783
Fragments arranged by size:
4783 4296 2385
AlwNI CAG_nnn'CTG
Cuts at: 2235 9207 10779 2235
Size: 6972 1572 2920
Fragments arranged by size:
6972 2920 1572
ApaI G_GGCC'C
Cuts at: 2019 3245 2019
Size: 1226 10238
ApaLI G'TGCA_C
Cuts at: 5393 6479 9521 10677 5393
Size: 1086 3042 1156 6180
Fragments arranged by size:
6180 3042 1156 1086
ApoI r'AATT_y
Cuts at: 1493 3740 4024 4307 5292 6619 7464 8423 8773
Size: 2247 284 283 985 1327 845 959 350
Cuts at: 8773 8999 9151 11260 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2247 2109 1697 1327 985 959 845 350
284 283 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3708 6332 10018 160
Size: 872 2676 2624 3686 1606
Fragments arranged by size:
3686 2676 2624 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11464
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2147 2411 2849 2960 3206 3238
Size: 73 197 7 264 438 111 246 32
Cuts at: 3238 6605 8789 1870
Size: 3367 2184 4545
Fragments arranged by size:
4545 3367 2184 438 264 246 197 111
73 32 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4358 4391 4358
Size: 33 11431
BamHI G'GATC_C
Cuts at: 5331 8794 9161 5331
Size: 3463 367 7634
Fragments arranged by size:
7634 3463 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2135 2415 2456 2687
Size: 325 217 627 326 22 280 41 231
Cuts at: 2687 2812 5092 5251 8785 10240 618
Size: 125 2280 159 3534 1455 1842
Fragments arranged by size:
3534 2280 1842 1455 627 326 325 280
231 217 159 125 41 22
BanII G_rGCy'C
Cuts at: 1391 2019 3245 8783 1391
Size: 628 1226 5538 4072
Fragments arranged by size:
5538 4072 1226 628
BbeI G_GCGC'C
Cuts at: 2117 2139 2419 2460 2691 2816 2117
Size: 22 280 41 231 125 10765
Fragments arranged by size:
10765 280 231 125 41 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3486 3646 4949 5381 7284 7506 7739
Size: 971 1587 160 1303 432 1903 222 233
Cuts at: 7739 8115 8201 8726 928
Size: 376 86 525 3666
Fragments arranged by size:
3666 1903 1587 1303 971 525 432 376
233 222 160 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5237 5271 9655 9689 1903
Size: 34 3300 34 4384 34 3678
Fragments arranged by size:
4384 3678 3300 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5245 7014 9356 10566 5245
Size: 1769 2342 1210 6143
Fragments arranged by size:
6143 2342 1769 1210
BclI T'GATC_A
Cuts at: 1548 4762 6643 8493 1548
Size: 3214 1881 1850 4519
Fragments arranged by size:
4519 3214 1881 1850
BfrBI ATG'CAT
Cuts at: 7000 7000
Size: 11464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2468 8252 10075
Size: 107 122 71 631 605 796 5784 1823
Cuts at: 10075 136
Size: 1525
Fragments arranged by size:
5784 1823 1525 796 631 605 122 107
71
BglII A'GATC_T
Cuts at: 11459 11459
Size: 11464
BlpI GC'TnA_GC
Cuts at: 2294 5205 6197 8881 2294
Size: 2911 992 2684 4877
Fragments arranged by size:
4877 2911 2684 992
Bme1580I G_kGCm'C
Cuts at: 1790 2019 2095 2555 2799 3245 5131 5397 5441
Size: 229 76 460 244 446 1886 266 44
Cuts at: 5441 6483 9525 10681 1790
Size: 1042 3042 1156 2573
Fragments arranged by size:
3042 2573 1886 1156 1042 460 446 266
244 229 76 44
BmgBI CAC'GTC
Cuts at: 1318 2385 3084 3778 5791 1318
Size: 1067 699 694 2013 6991
Fragments arranged by size:
6991 2013 1067 699 694
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2767 6442 9195
Size: 416 53 83 399 24 1350 3675 2753
Cuts at: 9195 10153 442
Size: 958 1753
Fragments arranged by size:
3675 2753 1753 1350 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 6384 4217
Size: 2167 9297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3107 6149 6671 7659 10124 1239
Size: 1868 3042 522 988 2465 2579
Fragments arranged by size:
3042 2579 2465 1868 988 522
Bpu10I CC'TnA_GC
Cuts at: 2045 2051 2216 2702 4985 6474 2045
Size: 6 165 486 2283 1489 7035
Fragments arranged by size:
7035 2283 1489 486 165 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6354 6466 7569 9589 10454 10752 6354
Size: 112 1103 2020 865 298 7066
Fragments arranged by size:
7066 2020 1103 865 298 112
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6593 10127 882
Size: 617 5094 3534 2219
Fragments arranged by size:
5094 3534 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3286 3352 5596 9255 493
Size: 711 2082 66 2244 3659 2702
Fragments arranged by size:
3659 2702 2244 2082 711 66
BsaBI GATnn'nnATC
Cuts at: 5498 5676 7184 7779 11458 5498
Size: 178 1508 595 3679 5504
Fragments arranged by size:
5504 3679 1508 595 178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 2136 2416 2457
Size: 53 83 186 936 581 22 280 41
Cuts at: 2457 2688 2813 2979 9271 9653 10288 275
Size: 231 125 166 6292 382 635 1451
Fragments arranged by size:
6292 1451 936 635 581 382 280 231
186 166 125 83 53 41 22
BsaWI w'CCGG_w
Cuts at: 6102 9897 10295 10569 10716 11127 6102
Size: 3795 398 274 147 411 6439
Fragments arranged by size:
6439 3795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2898 2928 3105 3135 5338
Size: 30 165 30 1495 30 177 30 2203
Cuts at: 5338 5368 1178
Size: 30 7274
Fragments arranged by size:
7274 2203 1495 177 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2117 2162 4461 6384 8570 1186
Size: 829 102 45 2299 1923 2186 4080
Fragments arranged by size:
4080 2299 2186 1923 829 102 45
BseYI C'CCAG_C
Cuts at: 2186 2523 2844 5140 5434 10667 11163 2186
Size: 337 321 2296 294 5233 496 2487
Fragments arranged by size:
5233 2487 2296 496 337 321 294
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2759 5131 6899 7835 1163
Size: 573 1023 2372 1768 936 4792
Fragments arranged by size:
4792 2372 1768 1023 936 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2062 2164 2224 2322 2371 2757
Size: 275 186 61 102 60 98 49 386
Cuts at: 2757 5921 8914 9675 10703 1540
Size: 3164 2993 761 1028 2301
Fragments arranged by size:
3164 2993 2301 1028 761 386 275 186
102 98 61 60 49
BsiHKAI G_wGCw'C
Cuts at: 3357 5397 6432 6483 7126 8049 9525 9610 10681
Size: 2040 1035 51 643 923 1476 85 1071
Cuts at: 10681 3357
Size: 4140
Fragments arranged by size:
4140 2040 1476 1071 1035 923 643 85
51
BsmI GAATG_Cn'
Cuts at: 3661 3704 5361 5457 6996 8975 9068 3661
Size: 43 1657 96 1539 1979 93 6057
Fragments arranged by size:
6057 1979 1657 1539 96 93 43
BsmBI CGTCTCn'nnnn_
Cuts at: 2110 2494 2110
Size: 384 11080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2095 2555 2799 3245 3357 5131
Size: 399 229 76 460 244 446 112 1774
Cuts at: 5131 5397 5441 6432 6483 7126 8049 8783 9525
Size: 266 44 991 51 643 923 734 742
Cuts at: 9525 9610 10681 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1774 1071 991 923 742 734 643
460 446 399 266 244 229 112 85
76 51 44
BspEI T'CCGG_A
Cuts at: 6102 11127 6102
Size: 5025 6439
BspHI T'CATG_A
Cuts at: 4272 5098 5265 9353 11083 4272
Size: 826 167 4088 1730 4653
Fragments arranged by size:
4653 4088 1730 826 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 2773 4112 4126 5472 6511 7435 8819
Size: 1511 418 1339 14 1346 1039 924 1384
Cuts at: 8819 844
Size: 3489
Fragments arranged by size:
3489 1511 1384 1346 1339 1039 924 418
14
BsrBI CCG'CTC
Cuts at: 1394 1823 2021 2220 9351 1394
Size: 429 198 199 7131 3507
Fragments arranged by size:
7131 3507 429 199 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 6195 7601 8586 9959 10133 65
Size: 1183 2831 2116 1406 985 1373 174 1396
Fragments arranged by size:
2831 2116 1406 1396 1373 1183 985 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 2317 10108 10295 1700
Size: 110 267 240 7791 187 2869
Fragments arranged by size:
7791 2869 267 240 187 110
BsrGI T'GTAC_A
Cuts at: 96 5043 96
Size: 4947 6517
BssHII G'CGCG_C
Cuts at: 1925 2360 2570 2759 2761 1925
Size: 435 210 189 2 10628
Fragments arranged by size:
10628 435 210 189 2
BssSI C'ACGA_G
Cuts at: 7051 9524 10536 7051
Size: 2473 1012 7979
Fragments arranged by size:
7979 2473 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 2550 6503 794
Size: 1441 315 3953 5755
Fragments arranged by size:
5755 3953 1441 315
BstBI TT'CG_AA
Cuts at: 10268 10268
Size: 11464
BstEII G'GTnAC_C
Cuts at: 1184 8324 1184
Size: 7140 4324
BstXI CCAn_nnnn'nTGG
Cuts at: 5074 5089 5074
Size: 15 11449
BstYI r'GATC_y
Cuts at: 1972 2836 3148 4489 5331 5515 6376 6682 7252
Size: 864 312 1341 842 184 861 306 570
Cuts at: 7252 7634 8794 9161 9546 9563 11015 11101 11113
Size: 382 1160 367 385 17 1452 86 12
Cuts at: 11113 11459 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1341 1160 864 861 842 570
385 382 367 346 312 306 184 86
17 12
BstZ17I GTA'TAC
Cuts at: 8311 8311
Size: 11464
Bsu36I CC'TnA_GG
Cuts at: 2129 6543 8201 2129
Size: 4414 1658 5392
Fragments arranged by size:
5392 4414 1658
BtgI C'CryG_G
Cuts at: 513 1065 2224 2245 2478 2516 2802 3679 4065
Size: 552 1159 21 233 38 286 877 386
Cuts at: 4065 513
Size: 7912
Fragments arranged by size:
7912 1159 877 552 386 286 233 38
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2752 3045 487
Size: 683 56 1526 293 8906
Fragments arranged by size:
8906 1526 683 293 56
BtsI GCAGTG_nn'
Cuts at: 4642 4687 5395 8980 9774 9794 4642
Size: 45 708 3585 794 20 6312
Fragments arranged by size:
6312 3585 794 708 45 20
ClaI AT'CG_AT
Cuts at: 1554 4218 1554
Size: 2664 8800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11429
DraI TTT'AAA
Cuts at: 8019 8768 8019
Size: 749 10715
DraIII CAC_nnn'GTG
Cuts at: 2550 5479 2550
Size: 2929 8535
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2285 7034 10471 809
Size: 507 969 4749 3437 1802
Fragments arranged by size:
4749 3437 1802 969 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 2161 2221
Size: 54 1425 50 275 18 229 102 60
Cuts at: 2221 2315 2319 2368 2754 4261 5484 7911 8070
Size: 94 4 49 386 1507 1223 2427 159
Cuts at: 8070 8911 9800 11429 8
Size: 841 889 1629 43
Fragments arranged by size:
2427 1629 1507 1425 1223 889 841 386
275 229 159 102 94 60 54 50
49 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2059 2161 2221 2319 2368 2754 8911
Size: 275 247 102 60 98 49 386 6157
Cuts at: 8911 1537
Size: 4090
Fragments arranged by size:
6157 4090 386 275 247 102 98 60
49
EarI CTCTTCn'nnn_
Cuts at: 1353 4631 5570 6210 7100 7114 7453 9394 1353
Size: 3278 939 640 890 14 339 1941 3423
Fragments arranged by size:
3423 3278 1941 939 890 640 339 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2423 2491 2729 10051 10425 10571
Size: 1064 138 834 68 238 7322 374 146
Cuts at: 10571 387
Size: 1280
Fragments arranged by size:
7322 1280 1064 834 374 238 146 138
68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3107 3280 3483 3640 4612 6149 6671
Size: 393 1475 173 203 157 972 1537 522
Cuts at: 6671 6895 7659 7872 8738 9527 10124 10911 11179
Size: 224 764 213 866 789 597 787 268
Cuts at: 11179 1239
Size: 1524
Fragments arranged by size:
1537 1524 1475 972 866 789 787 764
597 522 393 268 224 213 203 173
157
EcoO109I rG'GnC_Cy
Cuts at: 3242 3875 4910 4932 8449 3242
Size: 633 1035 22 3517 6257
Fragments arranged by size:
6257 3517 1035 633 22
EcoRI G'AATT_C
Cuts at: 4307 8773 9151 4307
Size: 4466 378 6620
Fragments arranged by size:
6620 4466 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4880 4880
Size: 11464
FseI GG_CCGG'CC
Cuts at: 2321 2321
Size: 11464
FspI TGC'GCA
Cuts at: 9970 9970
Size: 11464
HaeII r_GCGC'y
Cuts at: 1678 2117 2139 2211 2266 2301 2419 2460 2691
Size: 439 22 72 55 35 118 41 231
Cuts at: 2691 2816 5075 10611 1678
Size: 125 2259 5536 2531
Fragments arranged by size:
5536 2531 2259 439 231 125 118 72
55 41 35 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4456 4488 5491 5523 5823
Size: 32 135 32 2884 32 1003 32 300
Cuts at: 5823 5855 5903 5935 6082 6114 7229 7261 7635
Size: 32 48 32 147 32 1115 32 374
Cuts at: 7635 7667 8546 8578 10105 10137 10179 10211 1373
Size: 32 879 32 1527 32 42 32 2626
Fragments arranged by size:
2884 2626 1527 1115 1003 879 374 300
147 135 48 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 2593 6954 7834 8808 9054
Size: 988 281 406 249 4361 880 974 246
Cuts at: 9054 669
Size: 3079
Fragments arranged by size:
4361 3079 988 974 880 406 281 249
246
HindIII A'AGCT_T
Cuts at: 748 3573 7884 748
Size: 2825 4311 4328
Fragments arranged by size:
4328 4311 2825
HpaI GTT'AAC
Cuts at: 9054 9054
Size: 11464
KasI G'GCGC_C
Cuts at: 2113 2135 2415 2456 2687 2812 2113
Size: 22 280 41 231 125 10765
Fragments arranged by size:
10765 280 231 125 41 22
KpnI G_GTAC'C
Cuts at: 8789 8789
Size: 11464
MfeI C'AATT_G
Cuts at: 7717 9063 7717
Size: 1346 10118
MluI A'CGCG_T
Cuts at: 11340 11340
Size: 11464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3614 4688 6963 7361 10578 10762 1305
Size: 2309 1074 2275 398 3217 184 2007
Fragments arranged by size:
3217 2309 2275 2007 1074 398 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4263 5486 7913 8072 10
Size: 54 1425 343 2431 1223 2427 159 3402
Fragments arranged by size:
3402 2431 2427 1425 1223 343 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4562 4778 5894 6048 7055 7629 8474
Size: 648 3396 216 1116 154 1007 574 845
Cuts at: 8474 9422 9781 9940 518
Size: 948 359 159 2042
Fragments arranged by size:
3396 2042 1116 1007 948 845 648 574
359 216 159 154
MspA1I CmG'CkG
Cuts at: 1606 2071 2226 2480 2518 2743 3064 5382 5434
Size: 465 155 254 38 225 321 2318 52
Cuts at: 5434 7675 8880 9557 10705 10950 1606
Size: 2241 1205 677 1148 245 2120
Fragments arranged by size:
2318 2241 2120 1205 1148 677 465 321
254 245 225 155 52 38
NaeI GCC'GGC
Cuts at: 2319 2319
Size: 11464
NarI GG'CG_CC
Cuts at: 2114 2136 2416 2457 2688 2813 2114
Size: 22 280 41 231 125 10765
Fragments arranged by size:
10765 280 231 125 41 22
NcoI C'CATG_G
Cuts at: 513 1065 2245 3679 513
Size: 552 1180 1434 8298
Fragments arranged by size:
8298 1434 1180 552
NdeI CA'TA_TG
Cuts at: 387 5987 8527 387
Size: 5600 2540 3324
Fragments arranged by size:
5600 3324 2540
NgoMIV G'CCGG_C
Cuts at: 2317 2317
Size: 11464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11464
NotI GC'GGCC_GC
Cuts at: 2161 2221 2368 8911 2161
Size: 60 147 6543 4714
Fragments arranged by size:
6543 4714 147 60
NsiI A_TGCA'T
Cuts at: 7002 7002
Size: 11464
NspI r_CATG'y
Cuts at: 1132 1229 2710 3390 3633 3998 4588 5326 5657
Size: 97 1481 680 243 365 590 738 331
Cuts at: 5657 6118 6461 8674 8822 10367 1132
Size: 461 343 2213 148 1545 2229
Fragments arranged by size:
2229 2213 1545 1481 738 680 590 461
365 343 331 243 148 97
PasI CC'CwG_GG
Cuts at: 1348 3231 3853 6486 1348
Size: 1883 622 2633 6326
Fragments arranged by size:
6326 2633 1883 622
PciI A'CATG_T
Cuts at: 3386 4584 5322 6114 10363 3386
Size: 1198 738 792 4249 4487
Fragments arranged by size:
4487 4249 1198 792 738
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5317 1108
Size: 4209 7255
PmeI GTTT'AAAC
Cuts at: 8768 8768
Size: 11464
PmlI CAC'GTG
Cuts at: 3286 3352 3286
Size: 66 11398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8642 8674 9533 9565 11046 11078 8642
Size: 32 859 32 1481 32 9028
Fragments arranged by size:
9028 1481 859 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4910 4932 8449 4910
Size: 22 3517 7925
Fragments arranged by size:
7925 3517 22
PshAI GACnn'nnGTC
Cuts at: 1613 3650 1613
Size: 2037 9427
PsiI TTA'TAA
Cuts at: 7578 9034 7578
Size: 1456 10008
PspOMI G'GGCC_C
Cuts at: 2015 3241 2015
Size: 1226 10238
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5226 5258 5764 5796 5226
Size: 32 506 32 10894
Fragments arranged by size:
10894 506 32 32
PstI C_TGCA'G
Cuts at: 830 2352 3015 4123 4649 5250 8816 830
Size: 1522 663 1108 526 601 3566 3478
Fragments arranged by size:
3566 3478 1522 1108 663 601 526
PvuI CG_AT'CG
Cuts at: 2001 5921 2001
Size: 3920 7544
PvuII CAG'CTG
Cuts at: 2743 3064 5382 5434 2743
Size: 321 2318 52 8773
Fragments arranged by size:
8773 2318 321 52
SacII CC_GC'GG
Cuts at: 2227 2481 2519 2227
Size: 254 38 11172
Fragments arranged by size:
11172 254 38
SalI G'TCGA_C
Cuts at: 1936 2342 8806 1936
Size: 406 6464 4594
Fragments arranged by size:
6464 4594 406
SanDI GG'GwC_CC
Cuts at: 4910 4910
Size: 11464
SapI GCTCTTCn'nnn_
Cuts at: 4631 7114 4631
Size: 2483 8981
SbfI CC_TGCA'GG
Cuts at: 3015 4123 8816 3015
Size: 1108 4693 5663
Fragments arranged by size:
5663 4693 1108
ScaI AGT'ACT
Cuts at: 2777 4426 9712 11424 2777
Size: 1649 5286 1712 2817
Fragments arranged by size:
5286 2817 1712 1649
SexAI A'CCwGG_T
Cuts at: 8328 8328
Size: 11464
SfcI C'TryA_G
Cuts at: 826 1045 2348 3011 4119 4645 5246 7285 7548
Size: 219 1303 663 1108 526 601 2039 263
Cuts at: 7548 7789 8356 8812 9947 10628 10819 826
Size: 241 567 456 1135 681 191 1471
Fragments arranged by size:
2039 1471 1303 1135 1108 681 663 601
567 526 456 263 241 219 191
SfoI GGC'GCC
Cuts at: 2115 2137 2417 2458 2689 2814 2115
Size: 22 280 41 231 125 10765
Fragments arranged by size:
10765 280 231 125 41 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11464
SmaI CCC'GGG
Cuts at: 1872 2142 2413 8791 1872
Size: 270 271 6378 4545
Fragments arranged by size:
6378 4545 271 270
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2849 6369 6445 6695 7584 9568
Size: 197 926 906 3520 76 250 889 1984
Cuts at: 9568 10469 10731 820
Size: 901 262 1553
Fragments arranged by size:
3520 1984 1553 926 906 901 889 262
250 197 76
SnaBI TAC'GTA
Cuts at: 493 9255 493
Size: 8762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11464
SphI G_CATG'C
Cuts at: 1132 2710 3998 6461 8822 1132
Size: 1578 1288 2463 2361 3774
Fragments arranged by size:
3774 2463 2361 1578 1288
SrfI GCCC'GGGC
Cuts at: 2413 2413
Size: 11464
SspI AAT'ATT
Cuts at: 5 52 4453 7092 9388 5
Size: 47 4401 2639 2296 2081
Fragments arranged by size:
4401 2639 2296 2081 47
StuI AGG'CCT
Cuts at: 1517 2011 1517
Size: 494 10970
StyI C'CwwG_G
Cuts at: 513 1065 2245 2832 3358 3679 4751 5487 6722
Size: 552 1180 587 526 321 1072 736 1235
Cuts at: 6722 7369 8903 513
Size: 647 1534 3074
Fragments arranged by size:
3074 1534 1235 1180 1072 736 647 587
552 526 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3640 7789 9491 9508 9661 9846 10291
Size: 1255 2060 4149 1702 17 153 185 445
Cuts at: 10291 325
Size: 1498
Fragments arranged by size:
4149 2060 1702 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2775 3247 3867
Size: 274 80 33 51 1443 798 472 620
Cuts at: 3867 4424 5043 5228 9710 11422 96
Size: 557 619 185 4482 1712 138
Fragments arranged by size:
4482 1712 1443 798 620 619 557 472
274 185 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2898 2930 5323 5355 11424 11456 2898
Size: 32 2393 32 6069 32 2906
Fragments arranged by size:
6069 2906 2393 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2386 2981 4193 1316
Size: 1070 595 1212 8587
Fragments arranged by size:
8587 1212 1070 595
XbaI T'CTAG_A
Cuts at: 8800 8800
Size: 11464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3868 3868
Size: 11464
XhoI C'TCGA_G
Cuts at: 1943 2849 1943
Size: 906 10558
XmaI C'CCGG_G
Cuts at: 1870 2140 2411 8789 1870
Size: 270 271 6378 4545
Fragments arranged by size:
6378 4545 271 270
XmnI GAAnn'nnTTC
Cuts at: 7885 8063 9593 7885
Size: 178 1530 9756
Fragments arranged by size:
9756 1530 178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9272 276
Size: 53 83 186 936 7738 2468
Fragments arranged by size:
7738 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BsiWI EcoICRI EcoNI EcoRV
FspAI NruI PacI PfoI PspXI RsrII SacI SfiI
SwaI