Restriction map for
pFN21AE2642
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsiEI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I ClaI CpoI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I KasI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI TspRI XbaI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 1023 TCCGGA T^CCGGA
AcsI 912 GAATTT [GA]^AATT[CT]
AlwI 413 GGATC GGATC(4/5)
AocI 1146 CCTGAGG CC^T[ACGT]AGG
ApaI 680 GGGCCC GGGCC^C
ApoI 912 GAATTT [GA]^AATT[CT]
AseI 388 ATTAAT AT^TAAT
AsnI 388 ATTAAT AT^TAAT
AspHI 117 GTGCTC G[AT]GC[AT]^C
AspI 513 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 680 GGGCCC G[GA]GC[CT]^C
BbvCI 505 GCTGAGG CCTCAGC(-5/-2)
BcgI 810 CGAGCGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 846 CCCGG CC^[GC]GG
BlpI 855 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 855 GCTCAGC GC^T[ACGT]AGC
BseAI 1023 TCCGGA T^CCGGA
BsgI 712 GTGCAG GTGCAG(16/14)
BsiHKAI 117 GTGCTC G[AT]GC[AT]^C
BsmBI 87 GAGACG CGTCTC(1/5)
BsmFI 258 GTCCC GGGAC(10/14)
BspEI 1023 TCCGGA T^CCGGA
BsrBI 811 GAGCGG CCGCTC(-3/-3)
BsrFI 803 GCCGGT [GA]^CCGG[CT]
BstAPI 546 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1146 CCTGAGG CC^T[ACGT]AGG
CelII 855 GCTCAGC GC^T[ACGT]AGC
Cfr10I 803 GCCGGT [GA]^CCGG[CT]
DraI 13 TTTAAA TTT^AAA
Eco81I 1146 CCTGAGG CC^T[ACGT]AGG
HaeII 594 GGCGCT [GA]GCGC^[CT]
HincII 1123 GTTAAC GT[CT]^[GA]AC
HindII 1123 GTTAAC GT[CT]^[GA]AC
HindIII 1029 AAGCTT A^AGCTT
HpaI 1123 GTTAAC GTT^AAC
MroI 1023 TCCGGA T^CCGGA
MslI 882 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 846 CCCGG CC^[GC]GG
NspI 702 GCATGT [GA]CATG^[CT]
PaeR7I 298 CTCGAG C^TCGAG
PflFI 513 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 648 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 371 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 388 ATTAAT AT^TAAT
SbfI 219 CCTGCAGG CCTGCA^GG
Sse8387I 219 CCTGCAGG CCTGCA^GG
TfiI 44 GATTC G^A[AT]TC
Tth111I 513 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 648 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 939 CCAGTTTGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 298 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 454 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
538 CAGAAGCTG
AvaI 298 CTCGAG C^[CT]CG[GA]G
584 CCCGAG
BalI 638 TGGCCA TGG^CCA
665 TGGCCA
BmyI 117 GTGCTC G[AGT]GC[ACT]^C
680 GGGCCC
BsaI 934 GAGACC GGTCTC(1/5)
657 GGTCTC
BsaWI 599 TCCGGT [AT]^CCGG[AT]
1023 TCCGGA
BsmI 237 GAATGC GAATGC(1/-1)
750 GCATTC
BsoBI 298 CTCGAG C^[CT]CG[GA]G
584 CCCGAG
Bsp1286I 117 GTGCTC G[AGT]GC[ACT]^C
680 GGGCCC
Cac8I 491 GCAAGC GC[ACGT]^[ACGT]GC
743 GCCTGC
CfoI 315 GCGC GCG^C
595 GCGC
DraIII 4 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1048 CACAATGTG
EcoT14I 338 CCAAGG C^C[AT][AT]GG
1100 CCAAGG
FauI 262 CCCGC CCCGC(4/6)
813 GCGGG
HhaI 315 GCGC GCG^C
595 GCGC
HinP1I 315 GCGC G^CGC
595 GCGC
MluNI 638 TGGCCA TGG^CCA
665 TGGCCA
MscI 638 TGGCCA TGG^CCA
665 TGGCCA
MspA1I 105 CAGCTG C[AC]G^C[GT]G
427 CAGCTG
PleI 613 GACTC GAGTC(4/5)
1020 GACTC
PvuII 105 CAGCTG CAG^CTG
427 CAGCTG
SexAI 632 ACCTGGT A^CC[AT]GGT
959 ACCTGGT
SfaNI 817 GATGC GCATC(5/9)
1074 GATGC
StuI 741 AGGCCT AGG^CCT
1086 AGGCCT
StyI 338 CCAAGG C^C[AT][AT]GG
1100 CCAAGG
Tsp509I 618 AATT ^AATT
913 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AvaII BbsI
BbvI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BspMI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI TaqI Tru9I TseI
Tsp45I TthHB8I