Restriction map for
pFN21AE2642
(Circular) (Six-base) MAPSORT of: pFN21AE2642.seq Check: 948 from: 1 to: 5896
pFN21AE2642 5896 bp
With 182 enzymes: SgfI *
November 2, 2016 16:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2664 2710 2664
Size: 46 5850
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3239 1937
Size: 1302 4594
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2665 3138 3959 5343 5611 1632
Size: 678 355 473 821 1384 268 1917
Fragments arranged by size:
1917 1384 821 678 473 355 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 2891 1166
Size: 1725 4171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5896
AlwNI CAG_nnn'CTG
Cuts at: 2464 2548 3639 5211 2464
Size: 84 1091 1572 3149
Fragments arranged by size:
3149 1572 1091 84
ApaI G_GGCC'C
Cuts at: 2689 2689
Size: 5896
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 2917 3205 3431 3583 5692 1493
Size: 1424 288 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 288 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2394 4450 160
Size: 872 1362 2056 1606
Fragments arranged by size:
2056 1606 1362 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 2589 3221 1870
Size: 73 360 286 632 4545
Fragments arranged by size:
4545 632 360 286 73
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3217 4672 618
Size: 325 217 627 1430 1455 1842
Fragments arranged by size:
1842 1455 1430 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2689 3215 1391
Size: 1298 526 4072
Fragments arranged by size:
4072 1298 526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 2355 2448 2997 3126 928
Size: 971 399 57 93 549 129 3698
Fragments arranged by size:
3698 971 549 399 129 93 57
BbvCI CC'TCA_GC
Cuts at: 2511 2511
Size: 5896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2804 2838 4087 4121 1903
Size: 34 50 34 783 34 1249 34 3678
Fragments arranged by size:
3678 1249 783 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 4998 3788
Size: 1210 4686
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4507 136
Size: 107 122 71 631 605 2835 1525
Fragments arranged by size:
2835 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 2861 3313 2861
Size: 452 5444
Bme1580I G_kGCm'C
Cuts at: 1790 2689 3957 5113 1790
Size: 899 1268 1156 2573
Fragments arranged by size:
2573 1268 1156 899
BmgBI CAC'GTC
Cuts at: 1318 2096 2401 3124 1318
Size: 778 305 723 4090
Fragments arranged by size:
4090 778 723 305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2938 3627 4585
Size: 416 53 83 399 24 1521 689 958
Cuts at: 4585 442
Size: 1753
Fragments arranged by size:
1753 1521 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2456 2957 4556 1239
Size: 866 351 501 1599 2579
Fragments arranged by size:
2579 1599 866 501 351
Bpu10I CC'TnA_GC
Cuts at: 2511 2703 2511
Size: 192 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2190 3080 4021 4886 5184 2190
Size: 890 941 865 298 2902
Fragments arranged by size:
2902 941 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2668 2933 4559 882
Size: 617 1169 265 1626 2219
Fragments arranged by size:
2219 1626 1169 617 265
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3703 4085 4720 275
Size: 53 83 186 936 2170 382 635 1451
Fragments arranged by size:
2170 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2604 3028 4329 4727 5001 5148 5559 2604
Size: 424 1301 398 274 147 411 2941
Fragments arranged by size:
2941 1301 424 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2930 2960 1178
Size: 30 165 30 1527 30 4114
Fragments arranged by size:
4114 1527 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2049 2226 2321 2330 2372 2426 2789 1186
Size: 863 177 95 9 42 54 363 4293
Fragments arranged by size:
4293 863 363 177 95 54 42 9
BseYI C'CCAG_C
Cuts at: 2112 2298 2595 5099 5595 2112
Size: 186 297 2504 496 2413
Fragments arranged by size:
2504 2413 496 297 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2738 1163
Size: 573 1002 4321
Fragments arranged by size:
4321 1002 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3346 4107 5135 1540
Size: 275 186 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 3957 4042 5113 2126
Size: 1831 85 1071 2909
Fragments arranged by size:
2909 1831 1071 85
BsmI GAATG_Cn'
Cuts at: 2248 2755 3407 3500 2248
Size: 507 652 93 4644
Fragments arranged by size:
4644 652 507 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2086 2086
Size: 5896
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2689 3215 3957 4042 5113 1391
Size: 399 336 563 526 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 526 399 336 85
BspEI T'CCGG_A
Cuts at: 3028 5559 3028
Size: 2531 3365
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 2586 2664 2710 3251 844
Size: 1388 354 78 46 541 3489
Fragments arranged by size:
3489 1388 541 354 78 46
BsrBI CCG'CTC
Cuts at: 1394 1823 2818 3783 1394
Size: 429 995 965 3507
Fragments arranged by size:
3507 995 965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4391 4565 65
Size: 1183 3143 174 1396
Fragments arranged by size:
3143 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2808 4540 4727 1700
Size: 110 998 1732 187 2869
Fragments arranged by size:
2869 1732 998 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2557 794
Size: 1763 4133
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 3226 3593 3978 3995 5447 5533 5545 5891
Size: 1254 367 385 17 1452 86 12 346
Cuts at: 5891 1972
Size: 1977
Fragments arranged by size:
1977 1452 1254 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3152 3152
Size: 5896
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5344
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 3412 4206 4226 3412
Size: 794 20 5082
Fragments arranged by size:
5082 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 2020 3200 2020
Size: 1180 4716
DraIII CAC_nnn'GTG
Cuts at: 2014 3058 2014
Size: 1044 4852
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2455 2643 2670
Size: 54 1425 50 275 18 625 188 27
Cuts at: 2670 3343 4232 5861 8
Size: 673 889 1629 43
Fragments arranged by size:
1629 1425 889 673 625 275 188 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3826 1353
Size: 2473 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2291 2684 4483 4857 5003 387
Size: 1064 138 702 393 1799 374 146 1280
Fragments arranged by size:
1799 1280 1064 702 393 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2310 2456 2665 2957 3138 3959
Size: 393 473 205 146 209 292 181 821
Cuts at: 3959 4556 5343 5611 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 821 787 597 473 393 292 268
209 205 181 146
EcoO109I rG'GnC_Cy
Cuts at: 2116 2377 2685 2686 2116
Size: 261 308 1 5326
Fragments arranged by size:
5326 308 261 1
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 2603 5043 1678
Size: 925 2440 2531
Fragments arranged by size:
2531 2440 925
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 4537 4569 4611
Size: 32 135 32 1556 32 1377 32 42
Cuts at: 4611 4643 1373
Size: 32 2626
Fragments arranged by size:
2626 1556 1377 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3130 3240 3486 669
Size: 988 281 1192 110 246 3079
Fragments arranged by size:
3079 1192 988 281 246 110
HindIII A'AGCT_T
Cuts at: 748 3034 748
Size: 2286 3610
HpaI GTT'AAC
Cuts at: 3130 3486 3130
Size: 356 5540
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5010 5194 1305
Size: 3705 184 2007
Fragments arranged by size:
3705 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2645 2672 10
Size: 54 1425 343 813 27 3234
Fragments arranged by size:
3234 1425 813 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2891 3854 4213 4372 518
Size: 648 1725 963 359 159 2042
Fragments arranged by size:
2042 1725 963 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2434 3312 3989 5137 5382 1606
Size: 506 322 878 677 1148 245 2120
Fragments arranged by size:
2120 1148 878 677 506 322 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5344
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2711 3254 4799 1132
Size: 97 1482 543 1545 2229
Fragments arranged by size:
2229 1545 1482 543 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2659 1108
Size: 1551 4345
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2377
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PspOMI G'GGCC_C
Cuts at: 2685 2685
Size: 5896
PspXI vC'TCGA_Gb
Cuts at: 2303 2303
Size: 5896
PstI C_TGCA'G
Cuts at: 830 2078 2111 2229 2469 2553 3248 830
Size: 1248 33 118 240 84 695 3478
Fragments arranged by size:
3478 1248 695 240 118 84 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
PvuII CAG'CTG
Cuts at: 2112 2434 2112
Size: 322 5574
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SbfI CC_TGCA'GG
Cuts at: 2229 3248 2229
Size: 1019 4877
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SexAI A'CCwGG_T
Cuts at: 2637 2964 2637
Size: 327 5569
SfcI C'TryA_G
Cuts at: 826 1045 2074 2107 2225 2465 2549 2790 3244
Size: 219 1029 33 118 240 84 241 454
Cuts at: 3244 4379 5060 5251 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1029 681 454 241 240 219
191 118 84 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2205 2303 3095 4000 4901 5163
Size: 197 926 262 98 792 905 901 262
Cuts at: 5163 820
Size: 1553
Fragments arranged by size:
1553 926 905 901 792 262 262 197
98
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 3254 1132
Size: 2122 3774
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 2748 3093 1517
Size: 1231 345 4320
Fragments arranged by size:
4320 1231 345
StyI C'CwwG_G
Cuts at: 513 1065 2343 3105 3335 513
Size: 552 1278 762 230 3074
Fragments arranged by size:
3074 1278 762 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2088 2312 3923 3940 4093 4278 4723
Size: 1255 508 224 1611 17 153 185 445
Cuts at: 4723 325
Size: 1498
Fragments arranged by size:
1611 1498 1255 508 445 224 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2569 3180 4142
Size: 274 80 33 51 1443 592 611 962
Cuts at: 4142 5854 96
Size: 1712 138
Fragments arranged by size:
1712 1443 962 611 592 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3044 3076 5856 5888 3044
Size: 32 2780 32 3052
Fragments arranged by size:
3052 2780 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2521 1316
Size: 1205 4691
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2951 2951
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 2303 1943
Size: 360 5536
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI