Restriction map for
pFN21AB8874
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI Ksp632I
MaeI MaeII MamI MboII MfeI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
ApaI 376 GGGCCC GGGCC^C
AspHI 307 GAGCTC G[AT]GC[AT]^C
AvaII 462 GGACC G^G[AT]CC
BamHI 9 GGATCC G^GATCC
BlpI 48 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 307 GAGCTC G[AT]GC[AT]^C
BssSI 388 CTCGTG CACGAG(-5/-1)
BstNI 303 CCTGG CC^[AT]GG
BstYI 9 GGATCC [GA]^GATC[CT]
CelII 48 GCTGAGC GC^T[ACGT]AGC
DdeI 49 CTGAG C^T[ACGT]AG
EcoRII 303 CCTGG ^CC[AT]GG
KspI 372 CCGCGG CCGC^GG
MaeIII 145 GTGAC ^GT[ACGT]AC
MflI 9 GGATCC [GA]^GATC[CT]
MslI 384 CACGCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 303 CCTGG CC^[AT]GG
NaeI 396 GCCGGC GCC^GGC
NgoMIV 396 GCCGGC G^CCGGC
NlaIII 297 CATG CATG^
PvuII 198 CAGCTG CAG^CTG
SacI 307 GAGCTC GAGCT^C
SacII 372 CCGCGG CCGC^GG
SfaNI 117 GCATC GCATC(5/9)
SmaI 429 CCCGGG CCC^GGG
SmlI 154 CTCAAG C^T[CT][GA]AG
TaqI 311 TCGA T^CGA
Tsp45I 145 GTGAC ^GT[GC]AC
TthHB8I 311 TCGA T^CGA
XhoII 9 GGATCC [GA]^GATC[CT]
XmaCI 429 CCCGGG C^CCGGG
XmaI 429 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 164 GGCGCC G[GA]^CG[CT]C
393 GGCGCC
AlwI 10 GATCC GGATC(4/5)
9 GGATC
AvaI 429 CCCGGG C^[CT]CG[GA]G
435 CTCGGG
BanI 164 GGCGCC G^G[CT][GA]CC
393 GGCGCC
BbeI 164 GGCGCC GGCGC^C
393 GGCGCC
BpmI 256 CTGGAG CTGGAG(16/14)
304 CTGGAG
BsaHI 164 GGCGCC G[GA]^CG[CT]C
393 GGCGCC
BseRI 139 GAGGAG GAGGAG(10/8)
229 GAGGAG
BsoBI 429 CCCGGG C^[CT]CG[GA]G
435 CTCGGG
BsrFI 113 ACCGGC [GA]^CCGG[CT]
396 GCCGGC
BssHII 280 GCGCGC G^CGCGC
352 GCGCGC
BstXI 296 CCATGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
383 CCACGCTCGTGG
Cfr10I 113 ACCGGC [GA]^CCGG[CT]
396 GCCGGC
DpnII 10 GATC ^GATC
36 GATC
EaeI 362 TGGCCG [CT]^GGCC[GA]
398 CGGCCA
HgaI 313 GACGC GACGC(5/10)
404 GCGTC
Hin1I 164 GGCGCC G[GA]^CG[CT]C
393 GGCGCC
HphI 144 GGTGA GGTGA(8/7)
410 TCACC
KasI 164 GGCGCC G^GCGCC
393 GGCGCC
MboI 10 GATC ^GATC
36 GATC
NarI 164 GGCGCC GG^CGCC
393 GGCGCC
NdeII 10 GATC ^GATC
36 GATC
PstI 235 CTGCAG CTGCA^G
340 CTGCAG
Sau3AI 10 GATC ^GATC
36 GATC
SfcI 235 CTGCAG C^T[GA][CT]AG
340 CTGCAG
SfoI 164 GGCGCC GGC^GCC
393 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstUI Cac8I CfoI
Cfr13I DsaI FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI