Restriction map for
pFN21AB8874
(Linear) (Six-base) MAPSORT of: KIBB8874.seq Check: 4339 from: 1 to: 492
KIBB8874.orf
With 182 enzymes: SgfI *
March 22, 2012 15:44 ..
AleI CACnn'nnGTG
Cuts at: 0 388 492
Size: 388 104
ApaI G_GGCC'C
Cuts at: 0 380 492
Size: 380 112
AvaI C'yCGr_G
Cuts at: 0 429 435 492
Size: 429 6 57
Fragments arranged by size:
429 57 6
BamHI G'GATC_C
Cuts at: 0 9 492
Size: 9 483
BanI G'GyrC_C
Cuts at: 0 164 393 492
Size: 164 229 99
Fragments arranged by size:
229 164 99
BanII G_rGCy'C
Cuts at: 0 131 311 380 436 492
Size: 131 180 69 56 56
Fragments arranged by size:
180 131 69 56 56
BbeI G_GCGC'C
Cuts at: 0 168 397 492
Size: 168 229 95
Fragments arranged by size:
229 168 95
BlpI GC'TnA_GC
Cuts at: 0 49 492
Size: 49 443
Bme1580I G_kGCm'C
Cuts at: 0 380 492
Size: 380 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 325 492
Size: 277 48 167
Fragments arranged by size:
277 167 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 492
Size: 139 353
BsaHI Gr'CG_yC
Cuts at: 0 165 394 492
Size: 165 229 98
Fragments arranged by size:
229 165 98
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 244 492
Size: 154 90 248
Fragments arranged by size:
248 154 90
BsiHKAI G_wGCw'C
Cuts at: 0 311 492
Size: 311 181
Bsp1286I G_dGCh'C
Cuts at: 0 131 311 380 436 492
Size: 131 180 69 56 56
Fragments arranged by size:
180 131 69 56 56
BsrFI r'CCGG_y
Cuts at: 0 113 396 492
Size: 113 283 96
Fragments arranged by size:
283 113 96
BssHII G'CGCG_C
Cuts at: 0 280 352 492
Size: 280 72 140
Fragments arranged by size:
280 140 72
BssSI C'ACGA_G
Cuts at: 0 388 492
Size: 388 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 390 492
Size: 303 87 102
Fragments arranged by size:
303 102 87
BstYI r'GATC_y
Cuts at: 0 9 492
Size: 9 483
BtgI C'CryG_G
Cuts at: 0 183 359 372 453 471 492
Size: 183 176 13 81 18 21
Fragments arranged by size:
183 176 81 21 18 13
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 165 200 492
Size: 165 35 292
Fragments arranged by size:
292 165 35
EaeI y'GGCC_r
Cuts at: 0 362 398 492
Size: 362 36 94
Fragments arranged by size:
362 94 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 123 492
Size: 123 369
EcoICRI GAG'CTC
Cuts at: 0 309 492
Size: 309 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 325 492
Size: 277 48 167
Fragments arranged by size:
277 167 48
HaeII r_GCGC'y
Cuts at: 0 85 168 340 397 492
Size: 85 83 172 57 95
Fragments arranged by size:
172 95 85 83 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 492
Size: 1 32 459
Fragments arranged by size:
459 32 1
KasI G'GCGC_C
Cuts at: 0 164 393 492
Size: 164 229 99
Fragments arranged by size:
229 164 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 230 492
Size: 53 177 262
Fragments arranged by size:
262 177 53
MslI CAynn'nnrTG
Cuts at: 0 388 492
Size: 388 104
MspA1I CmG'CkG
Cuts at: 0 162 200 374 492
Size: 162 38 174 118
Fragments arranged by size:
174 162 118 38
NaeI GCC'GGC
Cuts at: 0 398 492
Size: 398 94
NarI GG'CG_CC
Cuts at: 0 165 394 492
Size: 165 229 98
Fragments arranged by size:
229 165 98
NgoMIV G'CCGG_C
Cuts at: 0 396 492
Size: 396 96
PspOMI G'GGCC_C
Cuts at: 0 376 492
Size: 376 116
PstI C_TGCA'G
Cuts at: 0 239 344 492
Size: 239 105 148
Fragments arranged by size:
239 148 105
PvuII CAG'CTG
Cuts at: 0 200 492
Size: 200 292
SacI G_AGCT'C
Cuts at: 0 311 492
Size: 311 181
SacII CC_GC'GG
Cuts at: 0 375 492
Size: 375 117
SfcI C'TryA_G
Cuts at: 0 235 340 492
Size: 235 105 152
Fragments arranged by size:
235 152 105
SfoI GGC'GCC
Cuts at: 0 166 395 492
Size: 166 229 97
Fragments arranged by size:
229 166 97
SmaI CCC'GGG
Cuts at: 0 431 492
Size: 431 61
SmlI C'TyrA_G
Cuts at: 0 154 492
Size: 154 338
XmaI C'CCGG_G
Cuts at: 0 429 492
Size: 429 63
Enzymes that do cut:
AleI ApaI AvaI BamHI BanI BanII BbeI BlpI
Bme1580I BpmI BpuEI BsaHI BseRI BsiHKAI Bsp1286I BsrFI
BssHII BssSI BstXI BstYI BtgI CspCI EaeI EciI
EcoICRI Eco57MI HaeII Hin4I KasI MmeI MslI MspA1I
NaeI NarI NgoMIV PspOMI PstI PvuII SacI SacII
SfcI SfoI SmaI SmlI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EagI EarI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI