Restriction map for
pFN21AE6451
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I ClaI DraI DraIII DrdI
Eam1105I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MflI MluI MroI MseI
MunI NcoI NdeI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
SalI SbfI ScaI SexAI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Alw44I 741 GTGCAC G^TGCAC
AlwI 297 GATCC GGATC(4/5)
Aor51HI 506 AGCGCT AGC^GCT
ApaLI 741 GTGCAC G^TGCAC
BbvCI 1369 GCTGAGG CCTCAGC(-5/-2)
BlpI 1443 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1443 GCTCAGC GC^T[ACGT]AGC
BsgI 553 CTGCAC GTGCAG(16/14)
BspHI 578 TCATGA T^CATGA
BstEII 81 GGTCACC G^GT[ACGT]ACC
BstPI 81 GGTCACC G^GT[ACGT]ACC
CelII 1443 GCTCAGC GC^T[ACGT]AGC
CpoI 1182 CGGACCG CG^G[AT]CCG
EarI 799 CTCTTC CTCTTC(1/4)
Eco47III 506 AGCGCT AGC^GCT
EcoO65I 81 GGTCACC G^GT[ACGT]ACC
EcoT14I 833 CCAAGG C^C[AT][AT]GG
Ksp632I 799 CTCTTC CTCTTC(1/4)
MaeII 953 ACGT A^CGT
MslI 228 CATCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 192 GCTAGC G^CTAGC
NotI 945 GCGGCCGC GC^GGCCGC
RcaI 578 TCATGA T^CATGA
RsrII 1182 CGGACCG CG^G[AT]CCG
SacI 328 GAGCTC GAGCT^C
SapI 798 GCTCTTC GCTCTTC(1/4)
SfiI 975 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 357 AGGCCT AGG^CCT
StyI 833 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 408 GTAC GT^AC
765 GTAC
AscI 903 GGCGCGCC GG^CGCGCC
1117 GGCGCGCC
AvaII 705 GGTCC G^G[AT]CC
1183 GGACC
BalI 431 TGGCCA TGG^CCA
983 TGGCCA
BbsI 121 GTCTTC GAAGAC(2/6)
1273 GTCTTC
BfaI 193 CTAG C^TAG
301 CTAG
BpuAI 121 GTCTTC GAAGAC(2/6)
1273 GTCTTC
BsrBI 484 CCGCTC CCGCTC(-3/-3)
415 GAGCGG
BsrI 1133 CCAGT ACTGG(1/-1)
1352 CCAGT
BssSI 232 CTCGTG CACGAG(-5/-1)
1156 CTCGTG
DpnII 297 GATC ^GATC
1230 GATC
DsaI 287 CCGCGG C^C[GA][CT]GG
1166 CCGCGG
EagI 946 CGGCCG C^GGCCG
1197 CGGCCG
EclXI 946 CGGCCG C^GGCCG
1197 CGGCCG
Eco52I 946 CGGCCG C^GGCCG
1197 CGGCCG
HinfI 1384 GACTC G^A[ACGT]TC
1504 GAGTC
KspI 287 CCGCGG CCGC^GG
1166 CCGCGG
MaeI 193 CTAG C^TAG
301 CTAG
MboI 297 GATC ^GATC
1230 GATC
MluNI 431 TGGCCA TGG^CCA
983 TGGCCA
MscI 431 TGGCCA TGG^CCA
983 TGGCCA
NdeII 297 GATC ^GATC
1230 GATC
PleI 1384 GACTC GAGTC(4/5)
1504 GAGTC
PstI 679 CTGCAG CTGCA^G
1087 CTGCAG
PvuII 496 CAGCTG CAG^CTG
682 CAGCTG
RsaI 408 GTAC GT^AC
765 GTAC
SacII 287 CCGCGG CCGC^GG
1166 CCGCGG
Sau3AI 297 GATC ^GATC
1230 GATC
SgrAI 624 CGCCGGCG C[GA]^CCGG[CT]G
715 CGCCGGCG
SmaI 592 CCCGGG CCC^GGG
1451 CCCGGG
XmaCI 592 CCCGGG C^CCGGG
1451 CCCGGG
XmaI 592 CCCGGG C^CCGGG
1451 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AspHI AvaI BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssHII BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NgoMIV NlaIII NlaIV Sau96I
ScrFI SfaNI SfcI SfoI TaqI
TseI Tsp45I TspRI TthHB8I XcmI