(Circular) (Six-base) MAPSORT of: pFN21AE6451.seq Check: 2935 from: 1 to: 6217 pFN21AE6451 6217 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3402 4280 5664 5932 1632 Size: 1770 878 1384 268 1917 Fragments arranged by size: 1917 1770 1384 878 268 AfeI AGC'GCT Cuts at: 2513 2513 Size: 6217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2503 2506 3344 3960 5532 2503 Size: 3 838 616 1572 3188 Fragments arranged by size: 3188 1572 838 616 3 ApaI G_GGCC'C Cuts at: 2039 2222 2609 2039 Size: 183 387 5647 Fragments arranged by size: 5647 387 183 ApaLI G'TGCA_C Cuts at: 2746 4274 5430 2746 Size: 1528 1156 3533 Fragments arranged by size: 3533 1528 1156 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AscI GG'CGCG_CC Cuts at: 2909 3123 2909 Size: 214 6003 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2597 2798 3456 3542 1870 Size: 73 654 201 658 86 4545 Fragments arranged by size: 4545 658 654 201 86 73 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2724 2924 3035 3538 Size: 325 217 627 285 652 200 111 503 Cuts at: 3538 4993 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 652 627 503 325 285 217 200 111 BanII G_rGCy'C Cuts at: 1391 2039 2222 2337 2553 2609 2805 3000 3399 Size: 648 183 115 216 56 196 195 399 Cuts at: 3399 3468 3536 1391 Size: 69 68 4072 Fragments arranged by size: 4072 648 399 216 196 195 183 115 69 68 56 BbeI G_GCGC'C Cuts at: 2076 2728 2928 3039 2076 Size: 652 200 111 5254 Fragments arranged by size: 5254 652 200 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 3271 928 Size: 971 220 1152 3874 Fragments arranged by size: 3874 1152 971 220 BbvCI CC'TCA_GC Cuts at: 3375 3375 Size: 6217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2576 2599 2722 Size: 107 122 71 631 605 904 23 123 Cuts at: 2722 2987 4828 136 Size: 265 1841 1525 Fragments arranged by size: 1841 1525 904 631 605 265 123 122 107 71 23 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3449 3634 3449 Size: 185 6032 Bme1580I G_kGCm'C Cuts at: 1790 2039 2222 2609 2750 2856 4278 5434 1790 Size: 249 183 387 141 106 1422 1156 2573 Fragments arranged by size: 2573 1422 1156 387 249 183 141 106 BmgBI CAC'GTC Cuts at: 1318 2959 1318 Size: 1641 4576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 3948 4906 Size: 416 53 83 399 24 1715 816 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1753 1715 958 816 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2201 1056 Size: 1145 5072 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2528 3452 3461 4877 1239 Size: 1112 177 924 9 1416 2579 Fragments arranged by size: 2579 1416 1112 924 177 9 Bpu10I CC'TnA_GC Cuts at: 3008 3375 3008 Size: 367 5850 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2157 2725 2865 Size: 53 83 186 936 540 84 568 140 Cuts at: 2865 2925 3036 4024 4406 5041 275 Size: 60 111 988 382 635 1451 Fragments arranged by size: 1451 988 936 635 568 540 382 186 140 111 84 83 60 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6217 BseYI C'CCAG_C Cuts at: 2052 2058 2068 2348 2404 3145 3272 5420 5916 Size: 6 10 280 56 741 127 2148 496 Cuts at: 5916 2052 Size: 2353 Fragments arranged by size: 2353 2148 741 496 280 127 56 10 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2543 1163 Size: 573 807 4837 Fragments arranged by size: 4837 807 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2615 2638 2755 2954 3205 3667 Size: 275 186 614 23 117 199 251 462 Cuts at: 3667 4428 5456 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 614 462 275 251 199 186 117 23 BsiHKAI G_wGCw'C Cuts at: 2337 2358 2750 3108 3183 3450 4278 4363 5434 Size: 21 392 358 75 267 828 85 1071 Cuts at: 5434 2337 Size: 3120 Fragments arranged by size: 3120 1071 828 392 358 267 85 75 21 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2222 2337 2358 2553 2609 2750 Size: 399 249 183 115 21 195 56 141 Cuts at: 2750 2805 2856 3000 3108 3183 3399 3450 3468 Size: 55 51 144 108 75 216 51 18 Cuts at: 3468 3536 4278 4363 5434 1391 Size: 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 249 216 195 183 144 141 115 108 85 75 68 56 55 51 51 21 18 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 2583 4106 5836 2583 Size: 1523 1730 2964 Fragments arranged by size: 2964 1730 1523 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2422 2491 4104 1394 Size: 429 599 69 1613 3507 Fragments arranged by size: 3507 1613 599 429 69 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 2159 2630 2721 3200 3251 4861 Size: 110 280 69 471 91 479 51 1610 Cuts at: 4861 5048 1700 Size: 187 2869 Fragments arranged by size: 2869 1610 479 471 280 187 110 91 69 51 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 2136 2909 3021 3023 3087 3123 1925 Size: 211 773 112 2 64 36 5019 Fragments arranged by size: 5019 773 211 112 64 36 2 BssSI C'ACGA_G Cuts at: 2237 3161 4277 5289 2237 Size: 924 1116 1012 3165 Fragments arranged by size: 3165 1116 1012 924 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 2086 1184 Size: 902 5315 BstXI CCAn_nnnn'nTGG Cuts at: 2446 2824 3145 2446 Size: 378 321 5518 Fragments arranged by size: 5518 378 321 BstYI r'GATC_y Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212 Size: 1575 367 385 17 1452 86 12 346 Cuts at: 6212 1972 Size: 1977 Fragments arranged by size: 1977 1575 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2292 3171 513 Size: 552 939 288 879 3559 Fragments arranged by size: 3559 939 879 552 288 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2560 2748 3733 4527 4547 2560 Size: 188 985 794 20 4230 Fragments arranged by size: 4230 985 794 188 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2436 2494 2951 Size: 54 1425 50 275 18 606 58 457 Cuts at: 2951 2988 3202 3249 3664 4553 6182 8 Size: 37 214 47 415 889 1629 43 Fragments arranged by size: 1629 1425 889 606 457 415 275 214 58 54 50 47 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2951 3202 3664 1537 Size: 275 1139 251 462 4090 Fragments arranged by size: 4090 1139 462 275 251 EarI CTCTTCn'nnn_ Cuts at: 1353 2810 4147 1353 Size: 1457 1337 3423 Fragments arranged by size: 3423 1457 1337 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2678 3366 4804 5178 5324 387 Size: 1064 138 1089 688 1438 374 146 1280 Fragments arranged by size: 1438 1280 1089 1064 688 374 146 138 EcoICRI GAG'CTC Cuts at: 2335 2335 Size: 6217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2528 3402 3452 3461 4280 4877 Size: 393 719 177 874 50 9 819 597 Cuts at: 4877 5664 5932 1239 Size: 787 268 1524 Fragments arranged by size: 1524 874 819 787 719 597 393 268 177 50 9 EcoO109I rG'GnC_Cy Cuts at: 2035 2036 2219 3436 2035 Size: 1 183 1217 4816 Fragments arranged by size: 4816 1217 183 1 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2013 2076 2515 2631 2728 2798 2928 3039 Size: 335 63 439 116 97 70 130 111 Cuts at: 3039 3456 5364 1678 Size: 417 1908 2531 Fragments arranged by size: 2531 1908 439 417 335 130 116 111 97 70 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373 Size: 32 135 32 3286 32 42 32 2626 Fragments arranged by size: 3286 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2072 2724 2924 3035 2072 Size: 652 200 111 5254 Fragments arranged by size: 5254 652 200 111 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5331 5515 1305 Size: 4026 184 2007 Fragments arranged by size: 4026 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2438 2990 10 Size: 54 1425 343 606 552 3237 Fragments arranged by size: 3237 1425 606 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 4175 4534 4693 518 Size: 648 1071 1938 359 159 2042 Fragments arranged by size: 2042 1938 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2294 2348 2503 2689 3173 3633 4310 5458 Size: 688 54 155 186 484 460 677 1148 Cuts at: 5458 5703 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 688 677 484 460 245 186 155 54 NaeI GCC'GGC Cuts at: 2161 2632 2723 3202 3253 2161 Size: 471 91 479 51 5125 Fragments arranged by size: 5125 479 471 91 51 NarI GG'CG_CC Cuts at: 2073 2725 2925 3036 2073 Size: 652 200 111 5254 Fragments arranged by size: 5254 652 200 111 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2159 2630 2721 3200 3251 2159 Size: 471 91 479 51 5125 Fragments arranged by size: 5125 479 471 91 51 NheI G'CTAG_C Cuts at: 1052 2197 1052 Size: 1145 5072 NotI GC'GGCC_GC Cuts at: 2951 3664 2951 Size: 713 5504 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2706 1348 Size: 1358 4859 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PfoI T'CCnGG_A Cuts at: 2328 2328 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2035 2218 2605 2035 Size: 183 387 5647 Fragments arranged by size: 5647 387 183 PstI C_TGCA'G Cuts at: 830 2688 3096 3569 830 Size: 1858 408 473 3478 Fragments arranged by size: 3478 1858 473 408 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2503 2689 2503 Size: 186 6031 RsrII CG'GwC_CG Cuts at: 3188 3188 Size: 6217 SacI G_AGCT'C Cuts at: 2337 2337 Size: 6217 SacII CC_GC'GG Cuts at: 2295 3174 2295 Size: 879 5338 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SapI GCTCTTCn'nnn_ Cuts at: 2810 2810 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2164 2684 3092 3513 3565 4700 5381 Size: 219 1119 520 408 421 52 1135 681 Cuts at: 5381 5572 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1119 681 520 421 408 219 191 52 SfiI GGCCn_nnn'nGGCC Cuts at: 2987 2987 Size: 6217 SfoI GGC'GCC Cuts at: 2074 2726 2926 3037 2074 Size: 652 200 111 5254 Fragments arranged by size: 5254 652 200 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 2630 2721 1700 Size: 930 91 5196 Fragments arranged by size: 5196 930 91 SmaI CCC'GGG Cuts at: 1872 2599 3458 3544 1872 Size: 727 859 86 4545 Fragments arranged by size: 4545 859 727 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 5222 5484 820 Size: 197 926 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SrfI GCCC'GGGC Cuts at: 2599 2599 Size: 6217 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2364 1517 Size: 847 5370 StyI C'CwwG_G Cuts at: 513 1065 2004 2838 3656 513 Size: 552 939 834 818 3074 Fragments arranged by size: 3074 939 834 818 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2170 4244 4261 4414 4599 5044 Size: 1255 470 120 2074 17 153 185 445 Cuts at: 5044 325 Size: 1498 Fragments arranged by size: 2074 1498 1255 470 445 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2412 4463 6175 Size: 274 80 33 51 1443 435 2051 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 2051 1712 1443 435 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2049 2326 2788 2049 Size: 277 462 5478 Fragments arranged by size: 5478 462 277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 2597 3456 3542 1870 Size: 727 859 86 4545 Fragments arranged by size: 4545 859 727 86 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI SanDI SexAI SwaI