(Linear) (Six-base) MAPSORT of: KIEE6451.seq Check: 9195 from: 1 to: 1512 KIEE6451.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1397 1512 Size: 1397 115 AfeI AGC'GCT Cuts at: 0 508 1512 Size: 508 1004 AlwNI CAG_nnn'CTG Cuts at: 0 498 501 1339 1512 Size: 498 3 838 173 Fragments arranged by size: 838 498 173 3 ApaI G_GGCC'C Cuts at: 0 34 217 604 1512 Size: 34 183 387 908 Fragments arranged by size: 908 387 183 34 ApaLI G'TGCA_C Cuts at: 0 741 1512 Size: 741 771 AscI GG'CGCG_CC Cuts at: 0 904 1118 1512 Size: 904 214 394 Fragments arranged by size: 904 394 214 AvaI C'yCGr_G Cuts at: 0 592 793 1451 1512 Size: 592 201 658 61 Fragments arranged by size: 658 592 201 61 BanI G'GyrC_C Cuts at: 0 67 719 919 1030 1512 Size: 67 652 200 111 482 Fragments arranged by size: 652 482 200 111 67 BanII G_rGCy'C Cuts at: 0 34 217 332 548 604 800 995 1394 Size: 34 183 115 216 56 196 195 399 Cuts at: 1394 1463 1512 Size: 69 49 Fragments arranged by size: 399 216 196 195 183 115 69 56 49 34 BbeI G_GCGC'C Cuts at: 0 71 723 923 1034 1512 Size: 71 652 200 111 478 Fragments arranged by size: 652 478 200 111 71 BbsI GAAGACnn'nnnn_ Cuts at: 0 114 1266 1512 Size: 114 1152 246 Fragments arranged by size: 1152 246 114 BbvCI CC'TCA_GC Cuts at: 0 1370 1512 Size: 1370 142 BglI GCCn_nnn'nGGC Cuts at: 0 571 594 717 982 1512 Size: 571 23 123 265 530 Fragments arranged by size: 571 530 265 123 23 BlpI GC'TnA_GC Cuts at: 0 1444 1512 Size: 1444 68 Bme1580I G_kGCm'C Cuts at: 0 34 217 604 745 851 1512 Size: 34 183 387 141 106 661 Fragments arranged by size: 661 387 183 141 106 34 BmgBI CAC'GTC Cuts at: 0 954 1512 Size: 954 558 BmrI ACTGGGnnnn_n' Cuts at: 0 1127 1512 Size: 1127 385 BmtI G_CTAG'C Cuts at: 0 196 1512 Size: 196 1316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 523 1447 1456 1512 Size: 346 177 924 9 56 Fragments arranged by size: 924 346 177 56 9 Bpu10I CC'TnA_GC Cuts at: 0 1003 1370 1512 Size: 1003 367 142 Fragments arranged by size: 1003 367 142 BsaHI Gr'CG_yC Cuts at: 0 68 152 720 860 920 1031 1512 Size: 68 84 568 140 60 111 481 Fragments arranged by size: 568 481 140 111 84 68 60 BseYI C'CCAG_C Cuts at: 0 47 53 63 343 399 1140 1267 1512 Size: 47 6 10 280 56 741 127 245 Fragments arranged by size: 741 280 245 127 56 47 10 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 1512 Size: 538 974 BsiEI CG_ry'CG Cuts at: 0 610 633 750 949 1200 1512 Size: 610 23 117 199 251 312 Fragments arranged by size: 610 312 251 199 117 23 BsiHKAI G_wGCw'C Cuts at: 0 332 353 745 1103 1178 1445 1512 Size: 332 21 392 358 75 267 67 Fragments arranged by size: 392 358 332 267 75 67 21 Bsp1286I G_dGCh'C Cuts at: 0 34 217 332 353 548 604 745 800 Size: 34 183 115 21 195 56 141 55 Cuts at: 800 851 995 1103 1178 1394 1445 1463 1512 Size: 51 144 108 75 216 51 18 49 Fragments arranged by size: 216 195 183 144 141 115 108 75 56 55 51 51 49 34 21 18 BspHI T'CATG_A Cuts at: 0 578 1512 Size: 578 934 BsrBI CCG'CTC Cuts at: 0 417 486 1512 Size: 417 69 1026 Fragments arranged by size: 1026 417 69 BsrFI r'CCGG_y Cuts at: 0 85 154 625 716 1195 1246 1512 Size: 85 69 471 91 479 51 266 Fragments arranged by size: 479 471 266 91 85 69 51 BssHII G'CGCG_C Cuts at: 0 131 904 1016 1018 1082 1118 1512 Size: 131 773 112 2 64 36 394 Fragments arranged by size: 773 394 131 112 64 36 2 BssSI C'ACGA_G Cuts at: 0 232 1156 1512 Size: 232 924 356 Fragments arranged by size: 924 356 232 BstEII G'GTnAC_C Cuts at: 0 81 1512 Size: 81 1431 BstXI CCAn_nnnn'nTGG Cuts at: 0 441 819 1140 1512 Size: 441 378 321 372 Fragments arranged by size: 441 378 372 321 BtgI C'CryG_G Cuts at: 0 287 1166 1512 Size: 287 879 346 Fragments arranged by size: 879 346 287 BtsI GCAGTG_nn' Cuts at: 0 555 743 1512 Size: 555 188 769 Fragments arranged by size: 769 555 188 EaeI y'GGCC_r Cuts at: 0 431 489 946 983 1197 1244 1512 Size: 431 58 457 37 214 47 268 Fragments arranged by size: 457 431 268 214 58 47 37 EagI C'GGCC_G Cuts at: 0 946 1197 1512 Size: 946 251 315 Fragments arranged by size: 946 315 251 EarI CTCTTCn'nnn_ Cuts at: 0 805 1512 Size: 805 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 673 1361 1512 Size: 673 688 151 Fragments arranged by size: 688 673 151 EcoICRI GAG'CTC Cuts at: 0 330 1512 Size: 330 1182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 523 1397 1447 1456 1512 Size: 346 177 874 50 9 56 Fragments arranged by size: 874 346 177 56 50 9 EcoO109I rG'GnC_Cy Cuts at: 0 30 31 214 1431 1512 Size: 30 1 183 1217 81 Fragments arranged by size: 1217 183 81 30 1 HaeII r_GCGC'y Cuts at: 0 8 71 510 626 723 793 923 1034 Size: 8 63 439 116 97 70 130 111 Cuts at: 1034 1451 1512 Size: 417 61 Fragments arranged by size: 439 417 130 116 111 97 70 63 61 8 KasI G'GCGC_C Cuts at: 0 67 719 919 1030 1512 Size: 67 652 200 111 482 Fragments arranged by size: 652 482 200 111 67 MscI TGG'CCA Cuts at: 0 433 985 1512 Size: 433 552 527 Fragments arranged by size: 552 527 433 MslI CAynn'nnrTG Cuts at: 0 232 1512 Size: 232 1280 MspA1I CmG'CkG Cuts at: 0 289 343 498 684 1168 1512 Size: 289 54 155 186 484 344 Fragments arranged by size: 484 344 289 186 155 54 NaeI GCC'GGC Cuts at: 0 156 627 718 1197 1248 1512 Size: 156 471 91 479 51 264 Fragments arranged by size: 479 471 264 156 91 51 NarI GG'CG_CC Cuts at: 0 68 720 920 1031 1512 Size: 68 652 200 111 481 Fragments arranged by size: 652 481 200 111 68 NgoMIV G'CCGG_C Cuts at: 0 154 625 716 1195 1246 1512 Size: 154 471 91 479 51 266 Fragments arranged by size: 479 471 266 154 91 51 NheI G'CTAG_C Cuts at: 0 192 1512 Size: 192 1320 NotI GC'GGCC_GC Cuts at: 0 946 1512 Size: 946 566 PasI CC'CwG_GG Cuts at: 0 701 1512 Size: 701 811 PfoI T'CCnGG_A Cuts at: 0 323 1512 Size: 323 1189 PspOMI G'GGCC_C Cuts at: 0 30 213 600 1512 Size: 30 183 387 912 Fragments arranged by size: 912 387 183 30 PstI C_TGCA'G Cuts at: 0 683 1091 1512 Size: 683 408 421 Fragments arranged by size: 683 421 408 PvuII CAG'CTG Cuts at: 0 498 684 1512 Size: 498 186 828 Fragments arranged by size: 828 498 186 RsrII CG'GwC_CG Cuts at: 0 1183 1512 Size: 1183 329 SacI G_AGCT'C Cuts at: 0 332 1512 Size: 332 1180 SacII CC_GC'GG Cuts at: 0 290 1169 1512 Size: 290 879 343 Fragments arranged by size: 879 343 290 SapI GCTCTTCn'nnn_ Cuts at: 0 805 1512 Size: 805 707 SfcI C'TryA_G Cuts at: 0 159 679 1087 1512 Size: 159 520 408 425 Fragments arranged by size: 520 425 408 159 SfiI GGCCn_nnn'nGGCC Cuts at: 0 982 1512 Size: 982 530 SfoI GGC'GCC Cuts at: 0 69 721 921 1032 1512 Size: 69 652 200 111 480 Fragments arranged by size: 652 480 200 111 69 SgrAI Cr'CCGG_yG Cuts at: 0 625 716 1512 Size: 625 91 796 Fragments arranged by size: 796 625 91 SmaI CCC'GGG Cuts at: 0 594 1453 1512 Size: 594 859 59 Fragments arranged by size: 859 594 59 SrfI GCCC'GGGC Cuts at: 0 594 1512 Size: 594 918 StuI AGG'CCT Cuts at: 0 359 1512 Size: 359 1153 StyI C'CwwG_G Cuts at: 0 833 1512 Size: 833 679 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 45 165 1512 Size: 45 120 1347 Fragments arranged by size: 1347 120 45 TatI w'GTAC_w Cuts at: 0 407 1512 Size: 407 1105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 44 321 783 1512 Size: 44 277 462 729 Fragments arranged by size: 729 462 277 44 XmaI C'CCGG_G Cuts at: 0 592 1451 1512 Size: 592 859 61 Fragments arranged by size: 859 592 61 Enzymes that do cut: AcuI AfeI AlwNI ApaI ApaLI AscI AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrFI BssHII BssSI BstEII BstXI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI PasI PfoI PspOMI PstI PvuII RsrII SacI SacII SapI SfcI SfiI SfoI SgrAI SmaI SrfI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI SalI SanDI SbfI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI