Restriction map for
pFN21AB0488
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BcgI BciVI BclI BlnI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EcoO65I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NcoI NdeI NheI NruI
NsiI PacI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SfiI SgrAI SnaBI SpeI SspI
SwaI Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflII 1675 CTTAAG C^TTAAG
AhdI 1876 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 743 GTGCAC G^TGCAC
AocI 385 CCTGAGG CC^T[ACGT]AGG
ApaLI 743 GTGCAC G^TGCAC
AspEI 1876 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1898 TGCGCA TGC^GCA
BbrPI 2827 CACGTG CAC^GTG
BfrI 1675 CTTAAG C^TTAAG
BglII 2807 AGATCT A^GATCT
BsaAI 2827 CACGTG [CT]AC^GT[GA]
BsaBI 243 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1208 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1127 TGTACA T^GTACA
BsrGI 1127 TGTACA T^GTACA
BstBI 752 TTCGAA TT^CGAA
Bsu36I 385 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1876 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 385 CCTGAGG CC^T[ACGT]AGG
FseI 2875 GGCCGGCC GGCCGG^CC
FspI 1898 TGCGCA TGC^GCA
MaeII 2828 ACGT A^CGT
MamI 243 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1676 TTAA T^TAA
MslI 2823 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 752 TTCGAA TT^CGAA
PaeR7I 490 CTCGAG C^TCGAG
PmaCI 2827 CACGTG CAC^GTG
PmlI 2827 CACGTG CAC^GTG
ScaI 1223 AGTACT AGT^ACT
SexAI 2103 ACCAGGT A^CC[AT]GGT
SfuI 752 TTCGAA TT^CGAA
SphI 1017 GCATGC GCATG^C
SspBI 1127 TGTACA T^GTACA
Tru9I 1676 TTAA T^TAA
XhoI 490 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Aor51HI 353 AGCGCT AGC^GCT
548 AGCGCT
AspI 832 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
870 GACGGCGTC
BalI 1142 TGGCCA TGG^CCA
2999 TGGCCA
BamHI 1211 GGATCC G^GATCC
2720 GGATCC
BbsI 1081 GAAGAC GAAGAC(2/6)
1612 GAAGAC
BbvCI 1416 CCTCAGC CCTCAGC(-5/-2)
2666 CCTCAGC
BlpI 195 GCTGAGC GC^T[ACGT]AGC
2689 GCTCAGC
Bpu1102I 195 GCTGAGC GC^T[ACGT]AGC
2689 GCTCAGC
BpuAI 1081 GAAGAC GAAGAC(2/6)
1612 GAAGAC
BsaI 1184 GAGACC GGTCTC(1/5)
1787 GGTCTC
BssSI 2074 CACGAG CACGAG(-5/-1)
3043 CTCGTG
CelII 195 GCTGAGC GC^T[ACGT]AGC
2689 GCTCAGC
Eco47III 353 AGCGCT AGC^GCT
548 AGCGCT
MluNI 1142 TGGCCA TGG^CCA
2999 TGGCCA
MscI 1142 TGGCCA TGG^CCA
2999 TGGCCA
NotI 2511 GCGGCCGC GC^GGCCGC
3014 GCGGCCGC
NspI 1017 GCATGC [GA]CATG^[CT]
1646 GCATGT
PflFI 832 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
870 GACGGCGTC
PpuMI 1497 GGGACCT [GA]G^G[AT]CC[CT]
2267 GGGACCT
TfiI 89 GAATC G^A[AT]TC
2019 GAATC
Tsp509I 401 AATT ^AATT
2293 AATT
Tth111I 832 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
870 GACGGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfoI SmaI SmlI Sse8387I
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI