(Circular) (Six-base) MAPSORT of: pFN21AB0488.seq Check: 198 from: 1 to: 7801 pFN21AB0488 7801 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3259 3386 4924 4957 3259 Size: 127 1538 33 6103 Fragments arranged by size: 6103 1538 127 33 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5611 278 Size: 53 83 186 936 4075 2468 Fragments arranged by size: 4075 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5144 1937 Size: 3207 4594 Acc65I G'GTAC_C Cuts at: 5122 5122 Size: 7801 AclI AA'CG_TT Cuts at: 1563 5928 6301 1563 Size: 4365 373 3063 Fragments arranged by size: 4365 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3628 4400 5864 7248 7516 1632 Size: 1996 772 1464 1384 268 1917 Fragments arranged by size: 1996 1917 1464 1384 772 268 AfeI AGC'GCT Cuts at: 2360 2555 2360 Size: 195 7606 AflII C'TTAA_G Cuts at: 820 1017 3680 820 Size: 197 2663 4941 Fragments arranged by size: 4941 2663 197 AflIII A'CryG_T Cuts at: 1203 6700 7677 1203 Size: 5497 977 1327 Fragments arranged by size: 5497 1327 977 AgeI A'CCGG_T Cuts at: 6632 6632 Size: 7801 AhdI GACnn_n'nnGTC Cuts at: 3886 6530 3886 Size: 2644 5157 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7801 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3287 1184 Size: 2103 5698 AlwNI CAG_nnn'CTG Cuts at: 2473 2527 2872 3007 3019 3073 4462 4679 5079 Size: 54 345 135 12 54 1389 217 400 Cuts at: 5079 5544 7116 2473 Size: 465 1572 3158 Fragments arranged by size: 3158 1572 1389 465 400 345 217 135 54 54 12 ApaI G_GGCC'C Cuts at: 2774 2935 3367 3836 2774 Size: 161 432 469 6739 Fragments arranged by size: 6739 469 432 161 ApaLI G'TGCA_C Cuts at: 2748 5858 7014 2748 Size: 3110 1156 3535 Fragments arranged by size: 3535 3110 1156 ApoI r'AATT_y Cuts at: 1493 5110 5336 5488 7597 1493 Size: 3617 226 152 2109 1697 Fragments arranged by size: 3617 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6355 160 Size: 872 5323 1606 Fragments arranged by size: 5323 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2481 2495 2935 3762 4248 4782 Size: 73 113 425 14 440 827 486 534 Cuts at: 4782 5126 1870 Size: 344 4545 Fragments arranged by size: 4545 827 534 486 440 425 344 113 73 14 BamHI G'GATC_C Cuts at: 3216 4725 5131 5498 3216 Size: 1509 406 367 5519 Fragments arranged by size: 5519 1509 406 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2241 3329 3374 4112 4286 Size: 325 217 627 454 1088 45 738 174 Cuts at: 4286 4356 4432 4570 4969 5122 6577 618 Size: 70 76 138 399 153 1455 1842 Fragments arranged by size: 1842 1455 1088 738 627 454 399 325 217 174 153 138 76 70 45 BanII G_rGCy'C Cuts at: 1391 2774 2935 2942 3367 3824 3836 4122 4312 Size: 1383 161 7 425 457 12 286 190 Cuts at: 4312 4393 4473 4687 5120 1391 Size: 81 80 214 433 4072 Fragments arranged by size: 4072 1383 457 433 425 286 214 190 161 81 80 12 7 BbeI G_GCGC'C Cuts at: 2245 4436 4574 2245 Size: 2191 138 5472 Fragments arranged by size: 5472 2191 138 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3093 3624 928 Size: 971 1194 531 5105 Fragments arranged by size: 5105 1194 971 531 BbvCI CC'TCA_GC Cuts at: 3422 4672 3422 Size: 1250 6551 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5992 6026 1903 Size: 34 50 34 3971 34 3678 Fragments arranged by size: 3971 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5693 6903 5693 Size: 1210 6591 BclI T'GATC_A Cuts at: 1548 1548 Size: 7801 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3327 4691 4887 Size: 107 122 71 631 605 1655 1364 196 Cuts at: 4887 4891 6412 136 Size: 4 1521 1525 Fragments arranged by size: 1655 1525 1521 1364 631 605 196 122 107 71 4 BglII A'GATC_T Cuts at: 4812 7796 4812 Size: 2984 4817 BlpI GC'TnA_GC Cuts at: 2201 4695 5218 2201 Size: 2494 523 4784 Fragments arranged by size: 4784 2494 523 Bme1580I G_kGCm'C Cuts at: 1790 2752 2774 2935 3332 3367 3836 4289 4296 Size: 962 22 161 397 35 469 453 7 Cuts at: 4296 4634 4667 4974 5862 7018 1790 Size: 338 33 307 888 1156 2573 Fragments arranged by size: 2573 1156 962 888 469 453 397 338 307 161 35 33 22 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7801 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2587 3489 3576 Size: 416 53 83 399 24 1170 902 87 Cuts at: 3576 5532 6490 442 Size: 1956 958 1753 Fragments arranged by size: 1956 1753 1170 958 902 416 399 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7801 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2683 3469 2683 Size: 786 7015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2537 2615 2756 3188 3302 3344 3365 Size: 1085 213 78 141 432 114 42 21 Cuts at: 3365 3995 4333 4487 4701 4790 6461 1239 Size: 630 338 154 214 89 1671 2579 Fragments arranged by size: 2579 1671 1085 630 432 338 214 213 154 141 114 89 78 42 21 Bpu10I CC'TnA_GC Cuts at: 2547 3422 3440 3507 4672 2547 Size: 875 18 67 1165 5676 Fragments arranged by size: 5676 1165 875 67 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2594 2699 3083 3368 5926 6791 7089 2594 Size: 105 384 285 2558 865 298 3306 Fragments arranged by size: 3306 2558 865 384 298 285 105 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3183 3798 6464 882 Size: 617 1684 615 2666 2219 Fragments arranged by size: 2666 2219 1684 617 615 BsaAI yAC'GTr Cuts at: 493 1204 4834 5592 493 Size: 711 3630 758 2702 Fragments arranged by size: 3630 2702 758 711 BsaBI GATnn'nnATC Cuts at: 2252 7795 2252 Size: 5543 2258 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2242 2879 4433 Size: 53 83 186 936 570 139 637 1554 Cuts at: 4433 4571 5608 5990 6625 275 Size: 138 1037 382 635 1451 Fragments arranged by size: 1554 1451 1037 936 637 635 570 382 186 139 138 83 53 BsaWI w'CCGG_w Cuts at: 3213 6234 6632 6906 7053 7464 3213 Size: 3021 398 274 147 411 3550 Fragments arranged by size: 3550 3021 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2729 2759 3029 3059 3968 Size: 30 165 30 1326 30 270 30 909 Cuts at: 3968 3998 4646 4676 1178 Size: 30 648 30 4303 Fragments arranged by size: 4303 1326 909 648 270 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2054 2087 2204 2207 2396 2513 2516 Size: 865 3 33 117 3 189 117 3 Cuts at: 2516 2672 2804 3068 3185 3388 5012 1186 Size: 156 132 264 117 203 1624 3975 Fragments arranged by size: 3975 1624 865 264 203 189 156 132 117 117 117 33 3 3 3 BseYI C'CCAG_C Cuts at: 2355 4002 4311 4604 4633 4774 4876 5084 7004 Size: 1647 309 293 29 141 102 208 1920 Cuts at: 7004 7500 2355 Size: 496 2656 Fragments arranged by size: 2656 1920 1647 496 309 293 208 141 102 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3050 4228 4497 4823 4985 1163 Size: 573 1314 1178 269 326 162 3979 Fragments arranged by size: 3979 1314 1178 573 326 269 162 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2927 4520 4886 5023 5251 6012 Size: 275 186 926 1593 366 137 228 761 Cuts at: 6012 7040 1540 Size: 1028 2301 Fragments arranged by size: 2301 1593 1028 926 761 366 275 228 186 137 BsiHKAI G_wGCw'C Cuts at: 2207 2496 2752 3416 4783 4941 5862 5947 7018 Size: 289 256 664 1367 158 921 85 1071 Cuts at: 7018 2207 Size: 2990 Fragments arranged by size: 2990 1367 1071 921 664 289 256 158 85 BsmI GAATG_Cn' Cuts at: 5312 5405 5312 Size: 93 7708 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2496 2752 2774 2935 2942 3332 Size: 399 417 289 256 22 161 7 390 Cuts at: 3332 3367 3416 3824 3836 4122 4289 4296 4312 Size: 35 49 408 12 286 167 7 16 Cuts at: 4312 4393 4473 4634 4667 4687 4783 4941 4974 Size: 81 80 161 33 20 96 158 33 Cuts at: 4974 5120 5862 5947 7018 1391 Size: 146 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 417 408 399 390 289 286 256 167 161 161 158 146 96 85 81 80 49 35 33 33 22 20 16 12 7 7 BspEI T'CCGG_A Cuts at: 7464 7464 Size: 7801 BspHI T'CATG_A Cuts at: 5690 7420 5690 Size: 1730 6071 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2700 3081 3099 3259 3386 4599 4608 4924 Size: 1856 381 18 160 127 1213 9 316 Cuts at: 4924 4957 5156 844 Size: 33 199 3489 Fragments arranged by size: 3489 1856 1213 381 316 199 160 127 33 18 9 BsrBI CCG'CTC Cuts at: 1394 1823 2506 3292 4489 5688 1394 Size: 429 683 786 1197 1199 3507 Fragments arranged by size: 3507 1199 1197 786 683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6296 6470 65 Size: 1183 5048 174 1396 Fragments arranged by size: 5048 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2922 3587 3603 4353 4881 6445 Size: 110 241 871 665 16 750 528 1564 Cuts at: 6445 6632 1700 Size: 187 2869 Fragments arranged by size: 2869 1564 871 750 665 528 241 187 110 16 BsrGI T'GTAC_A Cuts at: 96 3132 96 Size: 3036 4765 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7801 BssSI C'ACGA_G Cuts at: 4079 5048 5861 6873 4079 Size: 969 813 1012 5007 Fragments arranged by size: 5007 1012 969 813 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 2527 3028 4765 5079 794 Size: 1679 54 501 1737 314 3516 Fragments arranged by size: 3516 1737 1679 501 314 54 BstBI TT'CG_AA Cuts at: 2758 6605 2758 Size: 3847 3954 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7801 BstXI CCAn_nnnn'nTGG Cuts at: 2641 3157 4426 2641 Size: 516 1269 6016 Fragments arranged by size: 6016 1269 516 BstYI r'GATC_y Cuts at: 1972 3216 3638 4162 4194 4725 4812 5131 5498 Size: 1244 422 524 32 531 87 319 367 Cuts at: 5498 5883 5900 7352 7438 7450 7796 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1244 531 524 422 385 367 346 319 87 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 2391 2391 Size: 7801 BtgI C'CryG_G Cuts at: 513 1065 2266 4425 4545 4921 513 Size: 552 1201 2159 120 376 3393 Fragments arranged by size: 3393 2159 1201 552 376 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7062 Fragments arranged by size: 7062 683 56 BtsI GCAGTG_nn' Cuts at: 2750 2882 3050 4759 4922 5098 5317 6111 6131 Size: 132 168 1709 163 176 219 794 20 Cuts at: 6131 2750 Size: 4420 Fragments arranged by size: 4420 1709 794 219 176 168 163 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7801 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7766 DraI TTT'AAA Cuts at: 5105 5105 Size: 7801 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6808 809 Size: 507 5492 1802 Fragments arranged by size: 5492 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2924 3147 Size: 54 1425 50 275 18 306 788 223 Cuts at: 3147 3814 4517 4883 5004 5020 5248 6137 7766 Size: 667 703 366 121 16 228 889 1629 Cuts at: 7766 8 Size: 43 Fragments arranged by size: 1629 1425 889 788 703 667 366 306 275 228 223 121 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2924 4517 4883 5020 5248 1537 Size: 275 1112 1593 366 137 228 4090 Fragments arranged by size: 4090 1593 1112 366 275 228 137 EarI CTCTTCn'nnn_ Cuts at: 1353 2736 3709 4379 5731 1353 Size: 1383 973 670 1352 3423 Fragments arranged by size: 3423 1383 1352 973 670 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 2084 3302 3590 3919 6388 Size: 1064 138 444 51 1218 288 329 2469 Cuts at: 6388 6762 6908 387 Size: 374 146 1280 Fragments arranged by size: 2469 1280 1218 1064 444 374 329 288 146 138 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2537 2615 2756 3188 3302 3344 Size: 393 692 213 78 141 432 114 42 Cuts at: 3344 3365 3628 3995 4333 4400 4487 4701 4790 Size: 21 263 367 338 67 87 214 89 Cuts at: 4790 5864 6461 7248 7516 1239 Size: 1074 597 787 268 1524 Fragments arranged by size: 1524 1074 787 692 597 432 393 367 338 268 263 214 213 141 114 89 87 78 67 42 21 EcoNI CCTnn'n_nnAGG Cuts at: 2378 2909 3462 4713 2378 Size: 531 553 1251 5466 Fragments arranged by size: 5466 1251 553 531 EcoO109I rG'GnC_Cy Cuts at: 2387 2395 2770 2902 2931 2932 3277 3503 3832 Size: 8 375 132 29 1 345 226 329 Cuts at: 3832 4084 4273 4364 2387 Size: 252 189 91 5824 Fragments arranged by size: 5824 375 345 329 252 226 189 132 91 29 8 1 EcoRI G'AATT_C Cuts at: 5110 5488 5110 Size: 378 7423 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3658 4214 3658 Size: 556 7245 FseI GG_CCGG'CC Cuts at: 4885 4885 Size: 7801 FspI TGC'GCA Cuts at: 3905 6307 3905 Size: 2402 5399 HaeII r_GCGC'y Cuts at: 1678 2245 2362 2557 4436 4574 4679 6948 1678 Size: 567 117 195 1879 138 105 2269 2531 Fragments arranged by size: 2531 2269 1879 567 195 138 117 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 3851 3872 3883 Size: 32 135 32 1256 32 991 21 11 Cuts at: 3883 3904 4147 4179 6442 6474 6516 6548 1373 Size: 21 243 32 2263 32 42 32 2626 Fragments arranged by size: 2626 2263 1256 991 243 135 42 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2230 2561 4255 5145 5391 669 Size: 988 281 292 331 1694 890 246 3079 Fragments arranged by size: 3079 1694 988 890 331 292 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7801 HpaI GTT'AAC Cuts at: 5391 5391 Size: 7801 KasI G'GCGC_C Cuts at: 2241 4432 4570 2241 Size: 2191 138 5472 Fragments arranged by size: 5472 2191 138 KpnI G_GTAC'C Cuts at: 5126 5126 Size: 7801 MfeI C'AATT_G Cuts at: 5400 5400 Size: 7801 MluI A'CGCG_T Cuts at: 7677 7677 Size: 7801 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3441 6915 7099 1305 Size: 2136 3474 184 2007 Fragments arranged by size: 3474 2136 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3149 5006 10 Size: 54 1425 343 1317 1857 2805 Fragments arranged by size: 2805 1857 1425 1317 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4832 5759 6118 6277 518 Size: 648 3666 927 359 159 2042 Fragments arranged by size: 3666 2042 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2455 2461 2524 2698 2869 3355 3361 3491 Size: 849 6 63 174 171 486 6 130 Cuts at: 3491 4402 4459 4637 5217 5894 7042 7287 1606 Size: 911 57 178 580 677 1148 245 2120 Fragments arranged by size: 2120 1148 911 849 677 580 486 245 178 174 171 130 63 57 6 6 NaeI GCC'GGC Cuts at: 2053 2924 3589 3605 4355 4883 2053 Size: 871 665 16 750 528 4971 Fragments arranged by size: 4971 871 750 665 528 16 NarI GG'CG_CC Cuts at: 2242 4433 4571 2242 Size: 2191 138 5472 Fragments arranged by size: 5472 2191 138 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7249 NdeI CA'TA_TG Cuts at: 387 387 Size: 7801 NgoMIV G'CCGG_C Cuts at: 2051 2922 3587 3603 4353 4881 2051 Size: 871 665 16 750 528 4971 Fragments arranged by size: 4971 871 750 665 528 16 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7801 NotI GC'GGCC_GC Cuts at: 4517 5020 5248 4517 Size: 503 228 7070 Fragments arranged by size: 7070 503 228 NspI r_CATG'y Cuts at: 1132 1229 3026 3655 5159 6704 1132 Size: 97 1797 629 1504 1545 2229 Fragments arranged by size: 2229 1797 1545 1504 629 97 PasI CC'CwG_GG Cuts at: 1348 2780 3367 3827 4709 1348 Size: 1432 587 460 882 4440 Fragments arranged by size: 4440 1432 882 587 460 PciI A'CATG_T Cuts at: 6700 6700 Size: 7801 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7801 PfoI T'CCnGG_A Cuts at: 2292 4737 4857 2292 Size: 2445 120 5236 Fragments arranged by size: 5236 2445 120 PmeI GTTT'AAAC Cuts at: 5105 5105 Size: 7801 PmlI CAC'GTG Cuts at: 4834 4834 Size: 7801 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5870 5902 7383 7415 5870 Size: 32 1481 32 6256 Fragments arranged by size: 6256 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3503 4273 3503 Size: 770 7031 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7801 PsiI TTA'TAA Cuts at: 5371 5371 Size: 7801 PspOMI G'GGCC_C Cuts at: 2770 2931 3363 3832 2770 Size: 161 432 469 6739 Fragments arranged by size: 6739 469 432 161 PspXI vC'TCGA_Gb Cuts at: 2495 2495 Size: 7801 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4799 4831 4799 Size: 32 7769 PstI C_TGCA'G Cuts at: 830 2460 2478 2697 2910 3045 3078 3117 3360 Size: 1630 18 219 213 135 33 39 243 Cuts at: 3360 4441 5034 5153 830 Size: 1081 593 119 3478 Fragments arranged by size: 3478 1630 1081 593 243 219 213 135 119 39 33 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7801 PvuII CAG'CTG Cuts at: 2455 2698 3355 3491 4402 4459 2455 Size: 243 657 136 911 57 5797 Fragments arranged by size: 5797 911 657 243 136 57 SalI G'TCGA_C Cuts at: 1936 5143 1936 Size: 3207 4594 SbfI CC_TGCA'GG Cuts at: 3078 4441 5034 5153 3078 Size: 1363 593 119 5726 Fragments arranged by size: 5726 1363 593 119 ScaI AGT'ACT Cuts at: 3230 6049 7761 3230 Size: 2819 1712 3270 Fragments arranged by size: 3270 2819 1712 SexAI A'CCwGG_T Cuts at: 4108 4108 Size: 7801 SfcI C'TryA_G Cuts at: 826 1045 2456 2474 2693 2906 3041 3074 3113 Size: 219 1411 18 219 213 135 33 39 Cuts at: 3113 3356 4437 4504 5030 5149 6284 6965 7156 Size: 243 1081 67 526 119 1135 681 191 Cuts at: 7156 826 Size: 1471 Fragments arranged by size: 1471 1411 1135 1081 681 526 243 219 219 213 191 135 119 67 39 33 18 SfoI GGC'GCC Cuts at: 2243 4434 4572 2243 Size: 2191 138 5472 Fragments arranged by size: 5472 2191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7801 SmaI CCC'GGG Cuts at: 1872 2483 2937 4250 5128 1872 Size: 611 454 1313 878 4545 Fragments arranged by size: 4545 1313 878 611 454 SmlI C'TyrA_G Cuts at: 820 1017 1943 2495 2609 2714 3098 3383 3680 Size: 197 926 552 114 105 384 285 297 Cuts at: 3680 5905 6806 7068 820 Size: 2225 901 262 1553 Fragments arranged by size: 2225 1553 926 901 552 384 297 285 262 197 114 105 SnaBI TAC'GTA Cuts at: 493 5592 493 Size: 5099 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7801 SphI G_CATG'C Cuts at: 1132 3026 5159 1132 Size: 1894 2133 3774 Fragments arranged by size: 3774 2133 1894 SspI AAT'ATT Cuts at: 5 52 5725 5 Size: 47 5673 2081 Fragments arranged by size: 5673 2081 47 StuI AGG'CCT Cuts at: 1517 2288 3389 3753 4064 1517 Size: 771 1101 364 311 5254 Fragments arranged by size: 5254 1101 771 364 311 StyI C'CwwG_G Cuts at: 513 1065 3333 4689 5007 5240 513 Size: 552 2268 1356 318 233 3074 Fragments arranged by size: 3074 2268 1356 552 318 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4766 4951 5044 5828 5845 5998 6183 Size: 1255 3186 185 93 784 17 153 185 Cuts at: 6183 6628 325 Size: 445 1498 Fragments arranged by size: 3186 1498 1255 784 445 185 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2674 3132 3228 Size: 274 80 33 51 1443 697 458 96 Cuts at: 3228 6047 7759 96 Size: 2819 1712 138 Fragments arranged by size: 2819 1712 1443 697 458 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 2729 2761 3029 3061 3968 4000 4646 Size: 32 628 32 268 32 907 32 646 Cuts at: 4646 4678 7761 7793 2069 Size: 32 3083 32 2077 Fragments arranged by size: 3083 2077 907 646 628 268 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2840 2878 1316 Size: 1524 38 6239 Fragments arranged by size: 6239 1524 38 XbaI T'CTAG_A Cuts at: 5137 5137 Size: 7801 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3340 4612 4745 3340 Size: 1272 133 6396 Fragments arranged by size: 6396 1272 133 XhoI C'TCGA_G Cuts at: 1943 2495 1943 Size: 552 7249 XmaI C'CCGG_G Cuts at: 1870 2481 2935 4248 5126 1870 Size: 611 454 1313 878 4545 Fragments arranged by size: 4545 1313 878 611 454 XmnI GAAnn'nnTTC Cuts at: 5930 5930 Size: 7801 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5609 276 Size: 53 83 186 936 4075 2468 Fragments arranged by size: 4075 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI