Restriction map for
pFN21AB0488
(Linear) (Six-base) MAPSORT of: KIBB0488.seq Check: 4278 from: 1 to: 3096
KIBB0488.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1254 1381 2919 2952 3096
Size: 1254 127 1538 33 144
Fragments arranged by size:
1538 1254 144 127 33
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1623 2395 3096
Size: 1623 772 701
Fragments arranged by size:
1623 772 701
AfeI AGC'GCT
Cuts at: 0 355 550 3096
Size: 355 195 2546
Fragments arranged by size:
2546 355 195
AflII C'TTAA_G
Cuts at: 0 1675 3096
Size: 1675 1421
AhdI GACnn_n'nnGTC
Cuts at: 0 1881 3096
Size: 1881 1215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1282 3096
Size: 1282 1814
AlwNI CAG_nnn'CTG
Cuts at: 0 468 522 867 1002 1014 1068 2457 2674
Size: 468 54 345 135 12 54 1389 217
Cuts at: 2674 3074 3096
Size: 400 22
Fragments arranged by size:
1389 468 400 345 217 135 54 54
22 12
ApaI G_GGCC'C
Cuts at: 0 769 930 1362 1831 3096
Size: 769 161 432 469 1265
Fragments arranged by size:
1265 769 469 432 161
ApaLI G'TGCA_C
Cuts at: 0 743 3096
Size: 743 2353
AvaI C'yCGr_G
Cuts at: 0 51 476 490 930 1757 2243 2777 3096
Size: 51 425 14 440 827 486 534 319
Fragments arranged by size:
827 534 486 440 425 319 51 14
BamHI G'GATC_C
Cuts at: 0 1211 2720 3096
Size: 1211 1509 376
Fragments arranged by size:
1509 1211 376
BanI G'GyrC_C
Cuts at: 0 236 1324 1369 2107 2281 2351 2427 2565
Size: 236 1088 45 738 174 70 76 138
Cuts at: 2565 2964 3096
Size: 399 132
Fragments arranged by size:
1088 738 399 236 174 138 132 76
70 45
BanII G_rGCy'C
Cuts at: 0 769 930 937 1362 1819 1831 2117 2307
Size: 769 161 7 425 457 12 286 190
Cuts at: 2307 2388 2468 2682 3096
Size: 81 80 214 414
Fragments arranged by size:
769 457 425 414 286 214 190 161
81 80 12 7
BbeI G_GCGC'C
Cuts at: 0 240 2431 2569 3096
Size: 240 2191 138 527
Fragments arranged by size:
2191 527 240 138
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 1619 3096
Size: 1088 531 1477
Fragments arranged by size:
1477 1088 531
BbvCI CC'TCA_GC
Cuts at: 0 1417 2667 3096
Size: 1417 1250 429
Fragments arranged by size:
1417 1250 429
BglI GCCn_nnn'nGGC
Cuts at: 0 1322 2686 2882 2886 3096
Size: 1322 1364 196 4 210
Fragments arranged by size:
1364 1322 210 196 4
BglII A'GATC_T
Cuts at: 0 2807 3096
Size: 2807 289
BlpI GC'TnA_GC
Cuts at: 0 196 2690 3096
Size: 196 2494 406
Fragments arranged by size:
2494 406 196
Bme1580I G_kGCm'C
Cuts at: 0 747 769 930 1327 1362 1831 2284 2291
Size: 747 22 161 397 35 469 453 7
Cuts at: 2291 2629 2662 2969 3096
Size: 338 33 307 127
Fragments arranged by size:
747 469 453 397 338 307 161 127
35 33 22 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 582 1484 1571 3096
Size: 582 902 87 1525
Fragments arranged by size:
1525 902 582 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 678 1464 3096
Size: 678 786 1632
Fragments arranged by size:
1632 786 678
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 532 610 751 1183 1297 1339 1360
Size: 319 213 78 141 432 114 42 21
Cuts at: 1360 1990 2328 2482 2696 2785 3096
Size: 630 338 154 214 89 311
Fragments arranged by size:
630 432 338 319 311 214 213 154
141 114 89 78 42 21
Bpu10I CC'TnA_GC
Cuts at: 0 542 1417 1435 1502 2667 3096
Size: 542 875 18 67 1165 429
Fragments arranged by size:
1165 875 542 429 67 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 694 1078 1363 3096
Size: 589 105 384 285 1733
Fragments arranged by size:
1733 589 384 285 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 1178 1793 3096
Size: 1178 615 1303
Fragments arranged by size:
1303 1178 615
BsaAI yAC'GTr
Cuts at: 0 2829 3096
Size: 2829 267
BsaBI GATnn'nnATC
Cuts at: 0 247 3096
Size: 247 2849
BsaHI Gr'CG_yC
Cuts at: 0 98 237 874 2428 2566 3096
Size: 98 139 637 1554 138 530
Fragments arranged by size:
1554 637 530 139 138 98
BsaWI w'CCGG_w
Cuts at: 0 1208 3096
Size: 1208 1888
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 724 754 1024 1054 1963 1993 2641 2671
Size: 724 30 270 30 909 30 648 30
Cuts at: 2671 3096
Size: 425
Fragments arranged by size:
909 724 648 425 270 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 82 199 202 391 508 511
Size: 46 3 33 117 3 189 117 3
Cuts at: 511 667 799 1063 1180 1383 3007 3096
Size: 156 132 264 117 203 1624 89
Fragments arranged by size:
1624 264 203 189 156 132 117 117
117 89 46 33 3 3 3
BseYI C'CCAG_C
Cuts at: 0 350 1997 2306 2599 2628 2769 2871 3079
Size: 350 1647 309 293 29 141 102 208
Cuts at: 3079 3096
Size: 17
Fragments arranged by size:
1647 350 309 293 208 141 102 29
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 2223 2492 2818 2980 3096
Size: 1045 1178 269 326 162 116
Fragments arranged by size:
1178 1045 326 269 162 116
BsiEI CG_ry'CG
Cuts at: 0 922 2515 2881 3018 3096
Size: 922 1593 366 137 78
Fragments arranged by size:
1593 922 366 137 78
BsiHKAI G_wGCw'C
Cuts at: 0 202 491 747 1411 2778 2936 3096
Size: 202 289 256 664 1367 158 160
Fragments arranged by size:
1367 664 289 256 202 160 158
Bsp1286I G_dGCh'C
Cuts at: 0 202 491 747 769 930 937 1327 1362
Size: 202 289 256 22 161 7 390 35
Cuts at: 1362 1411 1819 1831 2117 2284 2291 2307 2388
Size: 49 408 12 286 167 7 16 81
Cuts at: 2388 2468 2629 2662 2682 2778 2936 2969 3096
Size: 80 161 33 20 96 158 33 127
Fragments arranged by size:
408 390 289 286 256 202 167 161
161 158 127 96 81 80 49 35
33 33 22 20 16 12 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 695 1076 1094 1254 1381 2594 2603 2919
Size: 695 381 18 160 127 1213 9 316
Cuts at: 2919 2952 3096
Size: 33 144
Fragments arranged by size:
1213 695 381 316 160 144 127 33
18 9
BsrBI CCG'CTC
Cuts at: 0 501 1287 2484 3096
Size: 501 786 1197 612
Fragments arranged by size:
1197 786 612 501
BsrFI r'CCGG_y
Cuts at: 0 46 917 1582 1598 2348 2876 3096
Size: 46 871 665 16 750 528 220
Fragments arranged by size:
871 750 665 528 220 46 16
BsrGI T'GTAC_A
Cuts at: 0 1127 3096
Size: 1127 1969
BssSI C'ACGA_G
Cuts at: 0 2074 3043 3096
Size: 2074 969 53
Fragments arranged by size:
2074 969 53
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 522 1023 2760 3074 3096
Size: 468 54 501 1737 314 22
Fragments arranged by size:
1737 501 468 314 54 22
BstBI TT'CG_AA
Cuts at: 0 753 3096
Size: 753 2343
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 1152 2421 3096
Size: 636 516 1269 675
Fragments arranged by size:
1269 675 636 516
BstYI r'GATC_y
Cuts at: 0 1211 1633 2157 2189 2720 2807 3096
Size: 1211 422 524 32 531 87 289
Fragments arranged by size:
1211 531 524 422 289 87 32
Bsu36I CC'TnA_GG
Cuts at: 0 386 3096
Size: 386 2710
BtgI C'CryG_G
Cuts at: 0 261 2420 2540 2916 3096
Size: 261 2159 120 376 180
Fragments arranged by size:
2159 376 261 180 120
BtsI GCAGTG_nn'
Cuts at: 0 745 877 1045 2754 2917 3093 3096
Size: 745 132 168 1709 163 176 3
Fragments arranged by size:
1709 745 176 168 163 132 3
EaeI y'GGCC_r
Cuts at: 0 131 919 1142 1809 2512 2878 2999 3015
Size: 131 788 223 667 703 366 121 16
Cuts at: 3015 3096
Size: 81
Fragments arranged by size:
788 703 667 366 223 131 121 81
16
EagI C'GGCC_G
Cuts at: 0 919 2512 2878 3015 3096
Size: 919 1593 366 137 81
Fragments arranged by size:
1593 919 366 137 81
EarI CTCTTCn'nnn_
Cuts at: 0 731 1704 2374 3096
Size: 731 973 670 722
Fragments arranged by size:
973 731 722 670
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 79 1297 1585 1914 3096
Size: 28 51 1218 288 329 1182
Fragments arranged by size:
1218 1182 329 288 51 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 532 610 751 1183 1297 1339 1360
Size: 319 213 78 141 432 114 42 21
Cuts at: 1360 1623 1990 2328 2395 2482 2696 2785 3096
Size: 263 367 338 67 87 214 89 311
Fragments arranged by size:
432 367 338 319 311 263 214 213
141 114 89 87 78 67 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 904 1457 2708 3096
Size: 373 531 553 1251 388
Fragments arranged by size:
1251 553 531 388 373
EcoO109I rG'GnC_Cy
Cuts at: 0 382 390 765 897 926 927 1272 1498
Size: 382 8 375 132 29 1 345 226
Cuts at: 1498 1827 2079 2268 2359 3096
Size: 329 252 189 91 737
Fragments arranged by size:
737 382 375 345 329 252 226 189
132 91 29 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1653 2209 3096
Size: 1653 556 887
Fragments arranged by size:
1653 887 556
FseI GG_CCGG'CC
Cuts at: 0 2880 3096
Size: 2880 216
FspI TGC'GCA
Cuts at: 0 1900 3096
Size: 1900 1196
HaeII r_GCGC'y
Cuts at: 0 240 357 552 2431 2569 2674 3096
Size: 240 117 195 1879 138 105 422
Fragments arranged by size:
1879 422 240 195 138 117 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1846 1867 1878 1899 2142 2174
Size: 823 32 991 21 11 21 243 32
Cuts at: 2174 3096
Size: 922
Fragments arranged by size:
991 922 823 243 32 32 21 21
11
HincII GTy'rAC
Cuts at: 0 225 556 2250 3096
Size: 225 331 1694 846
Fragments arranged by size:
1694 846 331 225
KasI G'GCGC_C
Cuts at: 0 236 2427 2565 3096
Size: 236 2191 138 531
Fragments arranged by size:
2191 531 236 138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1436 3096
Size: 1436 1660
MscI TGG'CCA
Cuts at: 0 1144 3001 3096
Size: 1144 1857 95
Fragments arranged by size:
1857 1144 95
MslI CAynn'nnrTG
Cuts at: 0 2827 3096
Size: 2827 269
MspA1I CmG'CkG
Cuts at: 0 450 456 519 693 864 1350 1356 1486
Size: 450 6 63 174 171 486 6 130
Cuts at: 1486 2397 2454 2632 3096
Size: 911 57 178 464
Fragments arranged by size:
911 486 464 450 178 174 171 130
63 57 6 6
NaeI GCC'GGC
Cuts at: 0 48 919 1584 1600 2350 2878 3096
Size: 48 871 665 16 750 528 218
Fragments arranged by size:
871 750 665 528 218 48 16
NarI GG'CG_CC
Cuts at: 0 237 2428 2566 3096
Size: 237 2191 138 530
Fragments arranged by size:
2191 530 237 138
NgoMIV G'CCGG_C
Cuts at: 0 46 917 1582 1598 2348 2876 3096
Size: 46 871 665 16 750 528 220
Fragments arranged by size:
871 750 665 528 220 46 16
NotI GC'GGCC_GC
Cuts at: 0 2512 3015 3096
Size: 2512 503 81
Fragments arranged by size:
2512 503 81
NspI r_CATG'y
Cuts at: 0 1021 1650 3096
Size: 1021 629 1446
Fragments arranged by size:
1446 1021 629
PasI CC'CwG_GG
Cuts at: 0 775 1362 1822 2704 3096
Size: 775 587 460 882 392
Fragments arranged by size:
882 775 587 460 392
PfoI T'CCnGG_A
Cuts at: 0 287 2732 2852 3096
Size: 287 2445 120 244
Fragments arranged by size:
2445 287 244 120
PmlI CAC'GTG
Cuts at: 0 2829 3096
Size: 2829 267
PpuMI rG'GwC_Cy
Cuts at: 0 1498 2268 3096
Size: 1498 770 828
Fragments arranged by size:
1498 828 770
PspOMI G'GGCC_C
Cuts at: 0 765 926 1358 1827 3096
Size: 765 161 432 469 1269
Fragments arranged by size:
1269 765 469 432 161
PspXI vC'TCGA_Gb
Cuts at: 0 490 3096
Size: 490 2606
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2794 2826 3096
Size: 2794 32 270
Fragments arranged by size:
2794 270 32
PstI C_TGCA'G
Cuts at: 0 455 473 692 905 1040 1073 1112 1355
Size: 455 18 219 213 135 33 39 243
Cuts at: 1355 2436 3029 3096
Size: 1081 593 67
Fragments arranged by size:
1081 593 455 243 219 213 135 67
39 33 18
PvuII CAG'CTG
Cuts at: 0 450 693 1350 1486 2397 2454 3096
Size: 450 243 657 136 911 57 642
Fragments arranged by size:
911 657 642 450 243 136 57
SbfI CC_TGCA'GG
Cuts at: 0 1073 2436 3029 3096
Size: 1073 1363 593 67
Fragments arranged by size:
1363 1073 593 67
ScaI AGT'ACT
Cuts at: 0 1225 3096
Size: 1225 1871
SexAI A'CCwGG_T
Cuts at: 0 2103 3096
Size: 2103 993
SfcI C'TryA_G
Cuts at: 0 451 469 688 901 1036 1069 1108 1351
Size: 451 18 219 213 135 33 39 243
Cuts at: 1351 2432 2499 3025 3096
Size: 1081 67 526 71
Fragments arranged by size:
1081 526 451 243 219 213 135 71
67 39 33 18
SfoI GGC'GCC
Cuts at: 0 238 2429 2567 3096
Size: 238 2191 138 529
Fragments arranged by size:
2191 529 238 138
SmaI CCC'GGG
Cuts at: 0 478 932 2245 3096
Size: 478 454 1313 851
Fragments arranged by size:
1313 851 478 454
SmlI C'TyrA_G
Cuts at: 0 490 604 709 1093 1378 1675 3096
Size: 490 114 105 384 285 297 1421
Fragments arranged by size:
1421 490 384 297 285 114 105
SphI G_CATG'C
Cuts at: 0 1021 3096
Size: 1021 2075
StuI AGG'CCT
Cuts at: 0 283 1384 1748 2059 3096
Size: 283 1101 364 311 1037
Fragments arranged by size:
1101 1037 364 311 283
StyI C'CwwG_G
Cuts at: 0 1328 2684 3002 3096
Size: 1328 1356 318 94
Fragments arranged by size:
1356 1328 318 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2761 2946 3039 3096
Size: 2761 185 93 57
Fragments arranged by size:
2761 185 93 57
TatI w'GTAC_w
Cuts at: 0 669 1127 1223 3096
Size: 669 458 96 1873
Fragments arranged by size:
1873 669 458 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 724 756 1024 1056 1963 1995
Size: 64 32 628 32 268 32 907 32
Cuts at: 1995 2641 2673 3096
Size: 646 32 423
Fragments arranged by size:
907 646 628 423 268 64 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 835 873 3096
Size: 835 38 2223
Fragments arranged by size:
2223 835 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1335 2607 2740 3096
Size: 1335 1272 133 356
Fragments arranged by size:
1335 1272 356 133
XhoI C'TCGA_G
Cuts at: 0 490 3096
Size: 490 2606
XmaI C'CCGG_G
Cuts at: 0 476 930 2243 3096
Size: 476 454 1313 853
Fragments arranged by size:
1313 853 476 454
Enzymes that do cut:
AarI AcuI AfeI AflII AhdI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI FseI FspI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PfoI PmlI PpuMI PspOMI PspXI PsrI PstI
PvuII SbfI ScaI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BssHII BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FspAI HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NruI
NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI
PvuI RsrII SacI SacII SalI SanDI SapI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI
XmnI ZraI