################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AclI AflII AflIII AhdI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BbrPI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FseI FspI HpaI MamI MfeI MluI MluNI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1599 GACGTC GACGT^C Acc65I 2102 GGTACC G^GTACC AccIII 1329 TCCGGA T^CCGGA AgeI 101 ACCGGT A^CCGGT Alw44I 1672 GTGCAC G^TGCAC ApaI 1377 GGGCCC GGGCC^C ApaLI 1672 GTGCAC G^TGCAC Asp700I 1080 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2102 GGTACC G^GTACC BbeI 1876 GGCGCC GGCGC^C BcgI 1725 CGACAAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1502 TGATCA T^GATCA BseAI 1329 TCCGGA T^CCGGA BsiEI 2238 CGACCG CG[GA][CT]^CG BspEI 1329 TCCGGA T^CCGGA BsrBI 1522 GAGCGG CCGCTC(-3/-3) BstEII 916 GGTGACC G^GT[ACGT]ACC BstPI 916 GGTGACC G^GT[ACGT]ACC EcoO65I 916 GGTGACC G^GT[ACGT]ACC EcoRV 781 GATATC GAT^ATC FbaI 1502 TGATCA T^GATCA HgaI 1167 GACGC GACGC(5/10) HincII 337 GTTGAC GT[CT]^[GA]AC HindII 337 GTTGAC GT[CT]^[GA]AC KasI 1876 GGCGCC G^GCGCC KpnI 2102 GGTACC GGTAC^C KspI 1367 CCGCGG CCGC^GG MroI 1329 TCCGGA T^CCGGA MslI 358 CATCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 2131 GCCGGC GCC^GGC NarI 1876 GGCGCC GG^CGCC NgoMIV 2131 GCCGGC G^CCGGC PinAI 101 ACCGGT A^CCGGT SacI 1903 GAGCTC GAGCT^C SacII 1367 CCGCGG CCGC^GG ScaI 1475 AGTACT AGT^ACT SexAI 1868 ACCAGGT A^CC[AT]GGT SfoI 1876 GGCGCC GGC^GCC SmlI 676 CTTGAG C^T[CT][GA]AG StuI 1145 AGGCCT AGG^CCT TfiI 403 GAATC G^A[AT]TC XcmI 1385 CCATGTGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1080 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1657 GTAGAC GT^[AC][GT]AC 1828 GTCTAC AcsI 226 AAATTC [GA]^AATT[CT] 301 AAATTC AcyI 1599 GACGTC G[GA]^CG[CT]C 1876 GGCGCC AlwI 480 GATCC GGATC(4/5) 2262 GATCC AocI 328 CCTGAGG CC^T[ACGT]AGG 1933 CCTCAGG Aor51HI 75 AGCGCT AGC^GCT 1754 AGCGCT ApoI 226 AAATTC [GA]^AATT[CT] 301 AAATTC AvaI 85 CCCGAG C^[CT]CG[GA]G 2141 CCCGAG BanII 1377 GGGCCC G[GA]GC[CT]^C 1903 GAGCTC BcnI 1092 CCGGG CC^[GC]GG 1668 CCGGG BglI 2138 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2194 GCCAACAAGGC BglII 986 AGATCT A^GATCT 1055 AGATCT BsaHI 1599 GACGTC G[GA]^CG[CT]C 1876 GGCGCC BsmBI 1601 CGTCTC CGTCTC(1/5) 1165 GAGACG BsoBI 85 CCCGAG C^[CT]CG[GA]G 2141 CCCGAG BstXI 627 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2072 CCAGGCGCATGG Bsu36I 328 CCTGAGG CC^T[ACGT]AGG 1933 CCTCAGG Eco47III 75 AGCGCT AGC^GCT 1754 AGCGCT Eco81I 328 CCTGAGG CC^T[ACGT]AGG 1933 CCTCAGG FauI 145 CCCGC CCCGC(4/6) 296 CCCGC Hin1I 1599 GACGTC G[GA]^CG[CT]C 1876 GGCGCC HindIII 673 AAGCTT A^AGCTT 907 AAGCTT MaeII 1600 ACGT A^CGT 1613 ACGT MseI 724 TTAA T^TAA 881 TTAA NciI 1092 CCGGG CC^[GC]GG 1668 CCGGG NcoI 2148 CCATGG C^CATGG 2231 CCATGG PflMI 761 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1438 CCAGACCTTGG PvuII 1014 CAGCTG CAG^CTG 1546 CAGCTG Tru9I 724 TTAA T^TAA 881 TTAA Van91I 761 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1438 CCAGACCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaII BanI BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII