(Circular) (Six-base) MAPSORT of: pFN21AE2083.seq Check: 7370 from: 1 to: 6976 pFN21AE2083 6976 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3493 3493 Size: 6976 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3608 4786 278 Size: 53 83 186 936 2072 1178 2468 Fragments arranged by size: 2468 2072 1178 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3663 3834 4319 1937 Size: 1726 171 485 4594 Fragments arranged by size: 4594 1726 485 171 Acc65I G'GTAC_C Cuts at: 4107 4297 4107 Size: 190 6786 AclI AA'CG_TT Cuts at: 1563 5103 5476 1563 Size: 3540 373 3063 Fragments arranged by size: 3540 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2594 2602 3041 3049 5039 6423 6691 1632 Size: 962 8 439 8 1990 1384 268 1917 Fragments arranged by size: 1990 1917 1384 962 439 268 8 8 AfeI AGC'GCT Cuts at: 2082 3761 2082 Size: 1679 5297 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6779 AflIII A'CryG_T Cuts at: 1203 5875 6852 1203 Size: 4672 977 1327 Fragments arranged by size: 4672 1327 977 AgeI A'CCGG_T Cuts at: 2106 5807 2106 Size: 3701 3275 AhdI GACnn_n'nnGTC Cuts at: 5705 5705 Size: 6976 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6976 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6976 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3074 3106 3074 Size: 32 6944 AlwNI CAG_nnn'CTG Cuts at: 2333 3899 3946 4719 6291 2333 Size: 1566 47 773 1572 3018 Fragments arranged by size: 3018 1572 1566 773 47 ApaI G_GGCC'C Cuts at: 3386 3386 Size: 6976 ApaLI G'TGCA_C Cuts at: 3677 5033 6189 3677 Size: 1356 1156 4464 Fragments arranged by size: 4464 1356 1156 ApoI r'AATT_y Cuts at: 1493 2231 2306 4285 4511 4663 6772 1493 Size: 738 75 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 738 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 5530 160 Size: 872 4498 1606 Fragments arranged by size: 4498 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 AvaI C'yCGr_G Cuts at: 1870 1943 2090 4146 4301 1870 Size: 73 147 2056 155 4545 Fragments arranged by size: 4545 2056 155 147 73 BamHI G'GATC_C Cuts at: 4306 4673 4306 Size: 367 6609 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 3793 3881 4107 4297 Size: 325 217 627 451 1555 88 226 190 Cuts at: 4297 5752 618 Size: 1455 1842 Fragments arranged by size: 1842 1555 1455 627 451 325 226 217 190 88 BanII G_rGCy'C Cuts at: 1391 3386 3912 4295 1391 Size: 1995 526 383 4072 Fragments arranged by size: 4072 1995 526 383 BbeI G_GCGC'C Cuts at: 3885 3885 Size: 6976 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2608 3135 3613 4005 928 Size: 971 161 548 527 478 392 3899 Fragments arranged by size: 3899 971 548 527 478 392 161 BbvCI CC'TCA_GC Cuts at: 2066 2326 3545 2066 Size: 260 1219 5497 Fragments arranged by size: 5497 1219 260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3719 3753 5167 5201 1903 Size: 34 50 34 1698 34 1414 34 3678 Fragments arranged by size: 3678 1698 1414 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4868 6078 4868 Size: 1210 5766 BclI T'GATC_A Cuts at: 1548 3507 1548 Size: 1959 5017 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4149 4205 5587 Size: 107 122 71 631 605 2477 56 1382 Cuts at: 5587 136 Size: 1525 Fragments arranged by size: 2477 1525 1382 631 605 122 107 71 56 BglII A'GATC_T Cuts at: 2991 3060 6971 2991 Size: 69 3911 2996 Fragments arranged by size: 3911 2996 69 BlpI GC'TnA_GC Cuts at: 4393 4393 Size: 6976 Bme1580I G_kGCm'C Cuts at: 1790 3386 3681 5037 6193 1790 Size: 1596 295 1356 1156 2573 Fragments arranged by size: 2573 1596 1356 1156 295 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6976 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2700 4707 5665 Size: 416 53 83 399 24 1283 2007 958 Cuts at: 5665 442 Size: 1753 Fragments arranged by size: 2007 1753 1283 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6976 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2468 2468 Size: 6976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2015 2516 2615 3176 3497 3542 5636 1239 Size: 776 501 99 561 321 45 2094 2579 Fragments arranged by size: 2579 2094 776 561 501 321 99 45 Bpu10I CC'TnA_GC Cuts at: 2066 2326 3545 3668 2066 Size: 260 1219 123 5374 Fragments arranged by size: 5374 1219 260 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2702 5101 5966 6264 2702 Size: 2399 865 298 3414 Fragments arranged by size: 3414 2399 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5639 882 Size: 617 4140 2219 Fragments arranged by size: 4140 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4767 493 Size: 711 3563 2702 Fragments arranged by size: 3563 2702 711 BsaBI GATnn'nnATC Cuts at: 6970 6970 Size: 6976 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3605 3882 4783 5165 Size: 53 83 186 936 2072 277 901 382 Cuts at: 5165 5800 275 Size: 635 1451 Fragments arranged by size: 2072 1451 936 901 635 382 277 186 83 53 BsaWI w'CCGG_w Cuts at: 2106 3334 4227 5409 5807 6081 6228 6639 2106 Size: 1228 893 1182 398 274 147 411 2443 Fragments arranged by size: 2443 1228 1182 893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2514 2544 3074 3104 1178 Size: 30 165 30 1111 30 530 30 5050 Fragments arranged by size: 5050 1111 530 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2699 2908 3281 3368 3539 3803 3992 Size: 991 522 209 373 87 171 264 189 Cuts at: 3992 4028 4267 1186 Size: 36 239 3895 Fragments arranged by size: 3895 991 522 373 264 239 209 189 171 87 36 BseYI C'CCAG_C Cuts at: 6179 6675 6179 Size: 496 6480 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2746 3759 1163 Size: 573 333 677 1013 4380 Fragments arranged by size: 4380 1013 677 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4246 4426 5187 6215 1540 Size: 275 186 2245 180 761 1028 2301 Fragments arranged by size: 2301 2245 1028 761 275 186 180 BsiHKAI G_wGCw'C Cuts at: 2505 3681 3695 3912 5037 5122 6193 2505 Size: 1176 14 217 1125 85 1071 3288 Fragments arranged by size: 3288 1176 1125 1071 217 85 14 BsmI GAATG_Cn' Cuts at: 4487 4580 4487 Size: 93 6883 BsmBI CGTCTCn'nnnn_ Cuts at: 3164 3612 3164 Size: 448 6528 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2505 3386 3681 3695 3912 4295 5037 Size: 399 715 881 295 14 217 383 742 Cuts at: 5037 5122 6193 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 715 399 383 295 217 85 14 BspEI T'CCGG_A Cuts at: 3334 6639 3334 Size: 3305 3671 BspHI T'CATG_A Cuts at: 4865 6595 4865 Size: 1730 5246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 2768 3444 3493 4331 844 Size: 1451 473 676 49 838 3489 Fragments arranged by size: 3489 1451 838 676 473 49 BsrBI CCG'CTC Cuts at: 1394 1823 3529 4863 1394 Size: 429 1706 1334 3507 Fragments arranged by size: 3507 1706 1334 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5471 5645 65 Size: 1183 4223 174 1396 Fragments arranged by size: 4223 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2106 4110 4136 5620 5807 1700 Size: 110 296 2004 26 1484 187 2869 Fragments arranged by size: 2869 2004 1484 296 187 110 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6976 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6976 BssSI C'ACGA_G Cuts at: 5036 6048 5036 Size: 1012 5964 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6976 BstBI TT'CG_AA Cuts at: 5780 5780 Size: 6976 BstEII G'GTnAC_C Cuts at: 1184 2921 1184 Size: 1737 5239 BstXI CCAn_nnnn'nTGG Cuts at: 2639 4084 2639 Size: 1445 5531 BstYI r'GATC_y Cuts at: 1972 2484 2991 3060 4306 4673 5058 5075 6527 Size: 512 507 69 1246 367 385 17 1452 Cuts at: 6527 6613 6625 6971 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1246 512 507 385 367 346 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 2334 3939 2334 Size: 1605 5371 BtgI C'CryG_G Cuts at: 513 1065 3120 3372 4153 4236 513 Size: 552 2055 252 781 83 3253 Fragments arranged by size: 3253 2055 781 552 252 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6237 Fragments arranged by size: 6237 683 56 BtsI GCAGTG_nn' Cuts at: 2525 3747 3797 4059 4137 4492 5286 5306 2525 Size: 1222 50 262 78 355 794 20 4195 Fragments arranged by size: 4195 1222 794 355 262 78 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6976 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4113 4148 529 Size: 35 3549 35 3357 Fragments arranged by size: 3549 3357 35 35 DraI TTT'AAA Cuts at: 4280 4280 Size: 6976 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5983 809 Size: 507 4667 1802 Fragments arranged by size: 4667 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3123 4134 4239 Size: 54 1425 50 275 18 1293 1011 105 Cuts at: 4239 4423 5312 6941 8 Size: 184 889 1629 43 Fragments arranged by size: 1629 1425 1293 1011 889 275 184 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4423 1537 Size: 275 2611 4090 Fragments arranged by size: 4090 2611 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2885 3042 3080 3119 3345 4906 1353 Size: 1532 157 38 39 226 1561 3423 Fragments arranged by size: 3423 1561 1532 226 157 39 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5563 5937 6083 387 Size: 1064 138 3974 374 146 1280 Fragments arranged by size: 3974 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3910 3910 Size: 6976 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2516 2594 2602 2615 3041 3049 Size: 393 383 501 78 8 13 426 8 Cuts at: 3049 3176 3497 3542 5039 5636 6423 6691 1239 Size: 127 321 45 1497 597 787 268 1524 Fragments arranged by size: 1524 1497 787 597 501 426 393 383 321 268 127 78 45 13 8 8 EcoNI CCTnn'n_nnAGG Cuts at: 2159 3452 3966 2159 Size: 1293 514 5169 Fragments arranged by size: 5169 1293 514 EcoO109I rG'GnC_Cy Cuts at: 2047 3383 3433 4045 2047 Size: 1336 50 612 4978 Fragments arranged by size: 4978 1336 612 50 EcoRI G'AATT_C Cuts at: 4285 4663 4285 Size: 378 6598 EcoRV GAT'ATC Cuts at: 2788 2788 Size: 6976 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2842 3071 2842 Size: 229 6747 FspI TGC'GCA Cuts at: 5482 5482 Size: 6976 HaeII r_GCGC'y Cuts at: 1678 2084 2359 3763 3885 6123 1678 Size: 406 275 1404 122 2238 2531 Fragments arranged by size: 2531 2238 1404 406 275 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3881 3913 5617 5649 5691 Size: 32 135 32 2309 32 1704 32 42 Cuts at: 5691 5723 1373 Size: 32 2626 Fragments arranged by size: 2626 2309 1704 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 4320 4566 669 Size: 988 281 406 1976 246 3079 Fragments arranged by size: 3079 1976 988 406 281 246 HindIII A'AGCT_T Cuts at: 748 2678 2912 748 Size: 1930 234 4812 Fragments arranged by size: 4812 1930 234 HpaI GTT'AAC Cuts at: 4566 4566 Size: 6976 KasI G'GCGC_C Cuts at: 3881 3881 Size: 6976 KpnI G_GTAC'C Cuts at: 4111 4301 4111 Size: 190 6786 MfeI C'AATT_G Cuts at: 4575 4575 Size: 6976 MluI A'CGCG_T Cuts at: 6852 6852 Size: 6976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6090 6274 1305 Size: 4785 184 2007 Fragments arranged by size: 4785 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5154 Fragments arranged by size: 5154 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 4934 5293 5452 518 Size: 648 1201 2567 359 159 2042 Fragments arranged by size: 2567 2042 1201 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3021 3374 3553 3754 4392 5069 6217 6462 Size: 1415 353 179 201 638 677 1148 245 Cuts at: 6462 1606 Size: 2120 Fragments arranged by size: 2120 1415 1148 677 638 353 245 201 179 NaeI GCC'GGC Cuts at: 4138 4138 Size: 6976 NarI GG'CG_CC Cuts at: 3882 3882 Size: 6976 NcoI C'CATG_G Cuts at: 513 1065 4153 4236 513 Size: 552 3088 83 3253 Fragments arranged by size: 3253 3088 552 83 NdeI CA'TA_TG Cuts at: 387 387 Size: 6976 NgoMIV G'CCGG_C Cuts at: 4136 4136 Size: 6976 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6976 NotI GC'GGCC_GC Cuts at: 4423 4423 Size: 6976 NspI r_CATG'y Cuts at: 1132 1229 2350 2395 2716 4334 5879 1132 Size: 97 1121 45 321 1618 1545 2229 Fragments arranged by size: 2229 1618 1545 1121 321 97 45 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6976 PciI A'CATG_T Cuts at: 5875 5875 Size: 6976 PflMI CCAn_nnn'nTGG Cuts at: 1108 2772 3449 1108 Size: 1664 677 4635 Fragments arranged by size: 4635 1664 677 PfoI T'CCnGG_A Cuts at: 2487 2721 2948 3096 3519 2487 Size: 234 227 148 423 5944 Fragments arranged by size: 5944 423 234 227 148 PmeI GTTT'AAAC Cuts at: 4280 4280 Size: 6976 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3074 3106 5045 5077 6558 6590 3074 Size: 32 1939 32 1481 32 3460 Fragments arranged by size: 3460 1939 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 3433 4045 2047 Size: 1386 612 4978 Fragments arranged by size: 4978 1386 612 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6976 PsiI TTA'TAA Cuts at: 4546 4546 Size: 6976 PspOMI G'GGCC_C Cuts at: 3382 3382 Size: 6976 PstI C_TGCA'G Cuts at: 830 3081 3147 3804 4054 4328 830 Size: 2251 66 657 250 274 3478 Fragments arranged by size: 3478 2251 657 274 250 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6976 PvuII CAG'CTG Cuts at: 3021 3553 3021 Size: 532 6444 SacI G_AGCT'C Cuts at: 3912 3912 Size: 6976 SacII CC_GC'GG Cuts at: 3375 3375 Size: 6976 SalI G'TCGA_C Cuts at: 1936 4318 1936 Size: 2382 4594 SbfI CC_TGCA'GG Cuts at: 4328 4328 Size: 6976 ScaI AGT'ACT Cuts at: 3482 5224 6936 3482 Size: 1742 1712 3522 Fragments arranged by size: 3522 1742 1712 SexAI A'CCwGG_T Cuts at: 3873 3873 Size: 6976 SfcI C'TryA_G Cuts at: 826 1045 2264 2575 3077 3143 3800 4050 4324 Size: 219 1219 311 502 66 657 250 274 Cuts at: 4324 5459 6140 6331 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1219 1135 681 657 502 311 274 250 219 191 66 SfoI GGC'GCC Cuts at: 3883 3883 Size: 6976 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6976 SmaI CCC'GGG Cuts at: 1872 4303 1872 Size: 2431 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2681 5080 5981 6243 820 Size: 197 926 738 2399 901 262 1553 Fragments arranged by size: 2399 1553 926 901 738 262 197 SnaBI TAC'GTA Cuts at: 493 4767 493 Size: 4274 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6976 SphI G_CATG'C Cuts at: 1132 4334 1132 Size: 3202 3774 SspI AAT'ATT Cuts at: 5 52 4900 5 Size: 47 4848 2081 Fragments arranged by size: 4848 2081 47 StuI AGG'CCT Cuts at: 1517 3152 1517 Size: 1635 5341 StyI C'CwwG_G Cuts at: 513 1065 3448 4153 4236 4415 513 Size: 552 2383 705 83 179 3074 Fragments arranged by size: 3074 2383 705 552 179 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 5003 5020 5173 5358 5803 325 Size: 1255 474 2949 17 153 185 445 1498 Fragments arranged by size: 2949 1498 1255 474 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3480 3771 5222 Size: 274 80 33 51 1443 1503 291 1451 Cuts at: 5222 6934 96 Size: 1712 138 Fragments arranged by size: 1712 1503 1451 1443 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2411 2425 2443 2514 2546 3341 3373 6936 Size: 18 14 18 71 32 795 32 3563 Cuts at: 6936 6968 2393 Size: 32 2401 Fragments arranged by size: 3563 2401 795 71 32 32 32 18 18 14 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6976 XbaI T'CTAG_A Cuts at: 4312 4312 Size: 6976 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3397 3397 Size: 6976 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6976 XmaI C'CCGG_G Cuts at: 1870 4301 1870 Size: 2431 4545 XmnI GAAnn'nnTTC Cuts at: 3089 5105 3089 Size: 2016 4960 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3606 4784 276 Size: 53 83 186 936 2072 1178 2468 Fragments arranged by size: 2468 2072 1178 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI