(Linear) (Six-base) MAPSORT of: KIEE2083.seq Check: 2849 from: 1 to: 2271 KIEE2083.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1488 2271 Size: 1488 783 AatII G_ACGT'C Cuts at: 0 1603 2271 Size: 1603 668 AccI GT'mk_AC Cuts at: 0 1658 1829 2271 Size: 1658 171 442 Fragments arranged by size: 1658 442 171 Acc65I G'GTAC_C Cuts at: 0 2102 2271 Size: 2102 169 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 597 1036 1044 2271 Size: 589 8 439 8 1227 Fragments arranged by size: 1227 589 439 8 8 AfeI AGC'GCT Cuts at: 0 77 1756 2271 Size: 77 1679 515 Fragments arranged by size: 1679 515 77 AgeI A'CCGG_T Cuts at: 0 101 2271 Size: 101 2170 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1069 1101 2271 Size: 1069 32 1170 Fragments arranged by size: 1170 1069 32 AlwNI CAG_nnn'CTG Cuts at: 0 328 1894 1941 2271 Size: 328 1566 47 330 Fragments arranged by size: 1566 330 328 47 ApaI G_GGCC'C Cuts at: 0 1381 2271 Size: 1381 890 ApaLI G'TGCA_C Cuts at: 0 1672 2271 Size: 1672 599 ApoI r'AATT_y Cuts at: 0 226 301 2271 Size: 226 75 1970 Fragments arranged by size: 1970 226 75 AvaI C'yCGr_G Cuts at: 0 85 2141 2271 Size: 85 2056 130 Fragments arranged by size: 2056 130 85 BanI G'GyrC_C Cuts at: 0 233 1788 1876 2102 2271 Size: 233 1555 88 226 169 Fragments arranged by size: 1555 233 226 169 88 BanII G_rGCy'C Cuts at: 0 1381 1907 2271 Size: 1381 526 364 Fragments arranged by size: 1381 526 364 BbeI G_GCGC'C Cuts at: 0 1880 2271 Size: 1880 391 BbsI GAAGACnn'nnnn_ Cuts at: 0 55 603 1130 1608 2000 2271 Size: 55 548 527 478 392 271 Fragments arranged by size: 548 527 478 392 271 55 BbvCI CC'TCA_GC Cuts at: 0 61 321 1540 2271 Size: 61 260 1219 731 Fragments arranged by size: 1219 731 260 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1714 1748 2271 Size: 1714 34 523 Fragments arranged by size: 1714 523 34 BclI T'GATC_A Cuts at: 0 1502 2271 Size: 1502 769 BglI GCCn_nnn'nGGC Cuts at: 0 2144 2200 2271 Size: 2144 56 71 Fragments arranged by size: 2144 71 56 BglII A'GATC_T Cuts at: 0 986 1055 2271 Size: 986 69 1216 Fragments arranged by size: 1216 986 69 Bme1580I G_kGCm'C Cuts at: 0 1381 1676 2271 Size: 1381 295 595 Fragments arranged by size: 1381 595 295 BmrI ACTGGGnnnn_n' Cuts at: 0 695 2271 Size: 695 1576 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 463 2271 Size: 463 1808 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 511 610 1171 1492 1537 2271 Size: 10 501 99 561 321 45 734 Fragments arranged by size: 734 561 501 321 99 45 10 Bpu10I CC'TnA_GC Cuts at: 0 61 321 1540 1663 2271 Size: 61 260 1219 123 608 Fragments arranged by size: 1219 608 260 123 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 697 2271 Size: 697 1574 BsaHI Gr'CG_yC Cuts at: 0 1600 1877 2271 Size: 1600 277 394 Fragments arranged by size: 1600 394 277 BsaWI w'CCGG_w Cuts at: 0 101 1329 2222 2271 Size: 101 1228 893 49 Fragments arranged by size: 1228 893 101 49 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 509 539 1069 1099 2271 Size: 509 30 530 30 1172 Fragments arranged by size: 1172 530 509 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 172 694 903 1276 1363 1534 1798 1987 Size: 172 522 209 373 87 171 264 189 Cuts at: 1987 2023 2262 2271 Size: 36 239 9 Fragments arranged by size: 522 373 264 239 209 189 172 171 87 36 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 741 1754 2271 Size: 64 677 1013 517 Fragments arranged by size: 1013 677 517 64 BsiEI CG_ry'CG Cuts at: 0 2241 2271 Size: 2241 30 BsiHKAI G_wGCw'C Cuts at: 0 500 1676 1690 1907 2271 Size: 500 1176 14 217 364 Fragments arranged by size: 1176 500 364 217 14 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1159 1607 2271 Size: 1159 448 664 Fragments arranged by size: 1159 664 448 Bsp1286I G_dGCh'C Cuts at: 0 500 1381 1676 1690 1907 2271 Size: 500 881 295 14 217 364 Fragments arranged by size: 881 500 364 295 217 14 BspEI T'CCGG_A Cuts at: 0 1329 2271 Size: 1329 942 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 290 763 1439 1488 2271 Size: 290 473 676 49 783 Fragments arranged by size: 783 676 473 290 49 BsrBI CCG'CTC Cuts at: 0 1524 2271 Size: 1524 747 BsrFI r'CCGG_y Cuts at: 0 101 2105 2131 2271 Size: 101 2004 26 140 Fragments arranged by size: 2004 140 101 26 BstEII G'GTnAC_C Cuts at: 0 916 2271 Size: 916 1355 BstXI CCAn_nnnn'nTGG Cuts at: 0 634 2079 2271 Size: 634 1445 192 Fragments arranged by size: 1445 634 192 BstYI r'GATC_y Cuts at: 0 479 986 1055 2271 Size: 479 507 69 1216 Fragments arranged by size: 1216 507 479 69 Bsu36I CC'TnA_GG Cuts at: 0 329 1934 2271 Size: 329 1605 337 Fragments arranged by size: 1605 337 329 BtgI C'CryG_G Cuts at: 0 1115 1367 2148 2231 2271 Size: 1115 252 781 83 40 Fragments arranged by size: 1115 781 252 83 40 BtsI GCAGTG_nn' Cuts at: 0 520 1742 1792 2054 2132 2271 Size: 520 1222 50 262 78 139 Fragments arranged by size: 1222 520 262 139 78 50 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2108 2143 2271 Size: 2108 35 128 Fragments arranged by size: 2108 128 35 EaeI y'GGCC_r Cuts at: 0 1118 2129 2234 2271 Size: 1118 1011 105 37 Fragments arranged by size: 1118 1011 105 37 EarI CTCTTCn'nnn_ Cuts at: 0 880 1037 1075 1114 1340 2271 Size: 880 157 38 39 226 931 Fragments arranged by size: 931 880 226 157 39 38 EcoICRI GAG'CTC Cuts at: 0 1905 2271 Size: 1905 366 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 511 589 597 610 1036 1044 1171 Size: 10 501 78 8 13 426 8 127 Cuts at: 1171 1492 1537 2271 Size: 321 45 734 Fragments arranged by size: 734 501 426 321 127 78 45 13 10 8 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 154 1447 1961 2271 Size: 154 1293 514 310 Fragments arranged by size: 1293 514 310 154 EcoO109I rG'GnC_Cy Cuts at: 0 42 1378 1428 2040 2271 Size: 42 1336 50 612 231 Fragments arranged by size: 1336 612 231 50 42 EcoRV GAT'ATC Cuts at: 0 783 2271 Size: 783 1488 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 837 1066 2271 Size: 837 229 1205 Fragments arranged by size: 1205 837 229 HaeII r_GCGC'y Cuts at: 0 79 354 1758 1880 2271 Size: 79 275 1404 122 391 Fragments arranged by size: 1404 391 275 122 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1876 1908 2271 Size: 1876 32 363 Fragments arranged by size: 1876 363 32 HincII GTy'rAC Cuts at: 0 339 2271 Size: 339 1932 HindIII A'AGCT_T Cuts at: 0 673 907 2271 Size: 673 234 1364 Fragments arranged by size: 1364 673 234 KasI G'GCGC_C Cuts at: 0 1876 2271 Size: 1876 395 KpnI G_GTAC'C Cuts at: 0 2106 2271 Size: 2106 165 MslI CAynn'nnrTG Cuts at: 0 362 2271 Size: 362 1909 MspA1I CmG'CkG Cuts at: 0 1016 1369 1548 1749 2271 Size: 1016 353 179 201 522 Fragments arranged by size: 1016 522 353 201 179 NaeI GCC'GGC Cuts at: 0 2133 2271 Size: 2133 138 NarI GG'CG_CC Cuts at: 0 1877 2271 Size: 1877 394 NcoI C'CATG_G Cuts at: 0 2148 2231 2271 Size: 2148 83 40 Fragments arranged by size: 2148 83 40 NgoMIV G'CCGG_C Cuts at: 0 2131 2271 Size: 2131 140 NspI r_CATG'y Cuts at: 0 345 390 711 2271 Size: 345 45 321 1560 Fragments arranged by size: 1560 345 321 45 PflMI CCAn_nnn'nTGG Cuts at: 0 767 1444 2271 Size: 767 677 827 Fragments arranged by size: 827 767 677 PfoI T'CCnGG_A Cuts at: 0 482 716 943 1091 1514 2271 Size: 482 234 227 148 423 757 Fragments arranged by size: 757 482 423 234 227 148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1069 1101 2271 Size: 1069 32 1170 Fragments arranged by size: 1170 1069 32 PpuMI rG'GwC_Cy Cuts at: 0 42 1428 2040 2271 Size: 42 1386 612 231 Fragments arranged by size: 1386 612 231 42 PspOMI G'GGCC_C Cuts at: 0 1377 2271 Size: 1377 894 PstI C_TGCA'G Cuts at: 0 1076 1142 1799 2049 2271 Size: 1076 66 657 250 222 Fragments arranged by size: 1076 657 250 222 66 PvuII CAG'CTG Cuts at: 0 1016 1548 2271 Size: 1016 532 723 Fragments arranged by size: 1016 723 532 SacI G_AGCT'C Cuts at: 0 1907 2271 Size: 1907 364 SacII CC_GC'GG Cuts at: 0 1370 2271 Size: 1370 901 ScaI AGT'ACT Cuts at: 0 1477 2271 Size: 1477 794 SexAI A'CCwGG_T Cuts at: 0 1868 2271 Size: 1868 403 SfcI C'TryA_G Cuts at: 0 259 570 1072 1138 1795 2045 2271 Size: 259 311 502 66 657 250 226 Fragments arranged by size: 657 502 311 259 250 226 66 SfoI GGC'GCC Cuts at: 0 1878 2271 Size: 1878 393 SmlI C'TyrA_G Cuts at: 0 676 2271 Size: 676 1595 StuI AGG'CCT Cuts at: 0 1147 2271 Size: 1147 1124 StyI C'CwwG_G Cuts at: 0 1443 2148 2231 2271 Size: 1443 705 83 40 Fragments arranged by size: 1443 705 83 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 49 2271 Size: 49 2222 TatI w'GTAC_w Cuts at: 0 1475 1766 2271 Size: 1475 291 505 Fragments arranged by size: 1475 505 291 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 388 406 420 438 509 541 1336 1368 Size: 388 18 14 18 71 32 795 32 Cuts at: 1368 2271 Size: 903 Fragments arranged by size: 903 795 388 71 32 32 18 18 14 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1392 2271 Size: 1392 879 XmnI GAAnn'nnTTC Cuts at: 0 1084 2271 Size: 1084 1187 ZraI GAC'GTC Cuts at: 0 1601 2271 Size: 1601 670 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AgeI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI KpnI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII ScaI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AclI AflII AflIII AhdI AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BlpI BmgBI BmtI BsaI BsaAI BsaBI BseYI BsiWI BsmI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoRI FseI FspI FspAI HpaI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI