Restriction map for
pFN21AE2374
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BanI BbeI BbsI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cac8I CelII ClaI CpoI DraI
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KpnI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 778 GTCTAC GT^[AC][GT]AC
AflIII 338 ACACGT A^C[GA][CT]GT
ApaI 51 GGGCCC GGGCC^C
BamHI 156 GGATCC G^GATCC
BbrPI 339 CACGTG CAC^GTG
BbvCI 558 GCTGAGG CCTCAGC(-5/-2)
BfaI 815 CTAG C^TAG
BglII 836 AGATCT A^GATCT
BsaBI 819 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 15 GTGCAG GTGCAG(16/14)
BspHI 821 TCATGA T^CATGA
BspMI 716 ACCTGC ACCTGC(4/8)
BsrFI 629 ACCGGC [GA]^CCGG[CT]
BssSI 322 CTCGTG CACGAG(-5/-1)
Cfr10I 629 ACCGGC [GA]^CCGG[CT]
DraIII 148 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 7 CCGCGG C^C[GA][CT]GG
EarI 756 GAAGAG CTCTTC(1/4)
EcoT14I 107 CCAAGG C^C[AT][AT]GG
HgaI 79 GCGTC GACGC(5/10)
HindIII 289 AAGCTT A^AGCTT
HphI 294 TCACC GGTGA(8/7)
Ksp632I 756 GAAGAG CTCTTC(1/4)
KspI 7 CCGCGG CCGC^GG
MaeI 815 CTAG C^TAG
MaeIII 28 GTGAC ^GT[ACGT]AC
MamI 819 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 502 CTCGAG C^TCGAG
PmaCI 339 CACGTG CAC^GTG
PmlI 339 CACGTG CAC^GTG
PpuMI 540 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 821 TCATGA T^CATGA
SacI 679 GAGCTC GAGCT^C
SacII 7 CCGCGG CCGC^GG
SmaI 54 CCCGGG CCC^GGG
StyI 107 CCAAGG C^C[AT][AT]GG
TfiI 380 GATTC G^A[AT]TC
Tsp45I 28 GTGAC ^GT[GC]AC
XcmI 287 CCAAGCTTCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 502 CTCGAG C^TCGAG
XmaCI 54 CCCGGG C^CCGGG
XmaI 54 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 441 GTAC GT^AC
627 GTAC
AhdI 73 GACGTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
715 GACCTGCAGTC
Alw44I 367 GTGCAC G^TGCAC
691 GTGCAC
AlwNI 469 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
510 CAGCTTCTG
ApaLI 367 GTGCAC G^TGCAC
691 GTGCAC
AspEI 73 GACGTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
715 GACCTGCAGTC
AvaII 357 GGACC G^G[AT]CC
541 GGTCC
BpmI 445 CTGGAG CTGGAG(16/14)
750 CTGGAG
BsaAI 339 CACGTG [CT]AC^GT[GA]
685 TACGTG
BseRI 499 CTCCTC GAGGAG(10/8)
730 GAGGAG
BsmAI 733 GAGAC GTCTC(1/5)
723 GTCTC
BsrBI 132 CCGCTC CCGCTC(-3/-3)
460 GAGCGG
DdeI 384 CTCAG C^T[ACGT]AG
559 CTGAG
Eam1105I 73 GACGTTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
715 GACCTGCAGTC
EcoNI 267 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
297 CCTGGATGAGG
MslI 21 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
295 CACCTGGATG
PleI 735 GACTC GAGTC(4/5)
811 GAGTC
RsaI 441 GTAC GT^AC
627 GTAC
SmlI 502 CTCGAG C^T[CT][GA]AG
574 CTCAAG
TspRI 365 CAGTG CA[GC]TG[ACGT][ACGT]^
650 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI CfoI Cfr13I
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TthHB8I XhoII