(Circular) (Six-base) MAPSORT of: pFN21AE2374.seq Check: 3846 from: 1 to: 5557 pFN21AE2374 5557 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2784 2900 1937 Size: 847 116 4594 Fragments arranged by size: 4594 847 116 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3620 5004 5272 1632 Size: 1988 1384 268 1917 Fragments arranged by size: 1988 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 2343 4456 5433 1203 Size: 1140 2113 977 1327 Fragments arranged by size: 2113 1327 1140 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 2083 2725 4286 2083 Size: 642 1561 3354 Fragments arranged by size: 3354 1561 642 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 2479 2520 3300 4872 2479 Size: 41 780 1572 3164 Fragments arranged by size: 3164 1572 780 41 ApaI G_GGCC'C Cuts at: 2060 2060 Size: 5557 ApaLI G'TGCA_C Cuts at: 2372 2696 3614 4770 2372 Size: 324 918 1156 3159 Fragments arranged by size: 3159 1156 918 324 ApoI r'AATT_y Cuts at: 1493 2866 3092 3244 5353 1493 Size: 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2059 2507 2619 2730 2882 1870 Size: 73 116 448 112 111 152 4545 Fragments arranged by size: 4545 448 152 116 112 111 73 BamHI G'GATC_C Cuts at: 2161 2887 3254 2161 Size: 726 367 4464 Fragments arranged by size: 4464 726 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2878 4333 618 Size: 325 217 627 1091 1455 1842 Fragments arranged by size: 1842 1455 1091 627 325 217 BanII G_rGCy'C Cuts at: 1391 2060 2620 2688 2876 1391 Size: 669 560 68 188 4072 Fragments arranged by size: 4072 669 560 188 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4586 BbvCI CC'TCA_GC Cuts at: 2564 2564 Size: 5557 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4168 136 Size: 107 122 71 631 605 2496 1525 Fragments arranged by size: 2496 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2841 5552 2841 Size: 2711 2846 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2060 2376 2700 3618 4774 1790 Size: 270 316 324 918 1156 2573 Fragments arranged by size: 2573 1156 918 324 316 270 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 3288 4246 Size: 416 53 83 399 24 1206 665 958 Cuts at: 4246 442 Size: 1753 Fragments arranged by size: 1753 1206 958 665 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 2776 4217 1239 Size: 1232 305 1441 2579 Fragments arranged by size: 2579 1441 1232 305 Bpu10I CC'TnA_GC Cuts at: 2564 2564 Size: 5557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 3682 4547 4845 2564 Size: 1118 865 298 3276 Fragments arranged by size: 3276 1118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2346 2692 3348 493 Size: 711 1142 346 656 2702 Fragments arranged by size: 2702 1142 711 656 346 BsaBI GATnn'nnATC Cuts at: 2828 5551 2828 Size: 2723 2834 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3364 3746 4381 275 Size: 53 83 186 936 1831 382 635 1451 Fragments arranged by size: 1831 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3990 4388 4662 4809 5220 3990 Size: 398 274 147 411 4327 Fragments arranged by size: 4327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5332 Fragments arranged by size: 5332 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 2750 1186 Size: 1309 255 3993 Fragments arranged by size: 3993 1309 255 BseYI C'CCAG_C Cuts at: 2050 2072 2401 2769 4760 5256 2050 Size: 22 329 368 1991 496 2351 Fragments arranged by size: 2351 1991 496 368 329 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2041 1163 Size: 573 305 4679 Fragments arranged by size: 4679 573 305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 3768 4796 1540 Size: 275 186 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2376 2688 2700 3618 3703 4774 2376 Size: 312 12 918 85 1071 3159 Fragments arranged by size: 3159 1071 918 312 85 12 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2376 2620 2688 2700 2876 3618 Size: 399 270 316 244 68 12 176 742 Cuts at: 3618 3703 4774 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 316 270 244 176 85 68 12 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 2826 3446 5176 2826 Size: 620 1730 3207 Fragments arranged by size: 3207 1730 620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2730 2845 2912 844 Size: 1886 115 67 3489 Fragments arranged by size: 3489 1886 115 67 BsrBI CCG'CTC Cuts at: 1394 1823 2139 2467 3444 1394 Size: 429 316 328 977 3507 Fragments arranged by size: 3507 977 429 328 316 BsrDI GCAATG_nn' Cuts at: 65 1248 4052 4226 65 Size: 1183 2804 174 1396 Fragments arranged by size: 2804 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2634 4201 4388 1700 Size: 110 824 1567 187 2869 Fragments arranged by size: 2869 1567 824 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 2327 3617 4629 2327 Size: 1290 1012 3255 Fragments arranged by size: 3255 1290 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2161 2223 2841 2887 3254 3639 3656 5108 Size: 189 62 618 46 367 385 17 1452 Cuts at: 5108 5194 5206 5552 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 618 385 367 346 189 86 62 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 513 Size: 552 939 8 4058 Fragments arranged by size: 4058 939 552 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 2376 2661 3073 3867 3887 2376 Size: 285 412 794 20 4046 Fragments arranged by size: 4046 794 412 285 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DraIII CAC_nnn'GTG Cuts at: 2158 2158 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3004 3893 5522 Size: 54 1425 50 275 18 1174 889 1629 Cuts at: 5522 8 Size: 43 Fragments arranged by size: 1629 1425 1174 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3004 1537 Size: 275 1192 4090 Fragments arranged by size: 4090 1192 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2756 3487 1353 Size: 1403 731 3423 Fragments arranged by size: 3423 1403 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4144 4518 4664 387 Size: 1064 138 2555 374 146 1280 Fragments arranged by size: 2555 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2686 2686 Size: 5557 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2471 2776 3620 4217 5004 5272 1239 Size: 393 839 305 844 597 787 268 1524 Fragments arranged by size: 1524 844 839 787 597 393 305 268 EcoNI CCTnn'n_nnAGG Cuts at: 2276 2306 2276 Size: 30 5527 EcoO109I rG'GnC_Cy Cuts at: 2056 2063 2546 2056 Size: 7 483 5067 Fragments arranged by size: 5067 483 7 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 4704 1678 Size: 3026 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 2165 2197 2711 Size: 32 135 32 497 32 64 32 514 Cuts at: 2711 2743 2833 2865 4198 4230 4272 4304 1373 Size: 32 90 32 1333 32 42 32 2626 Fragments arranged by size: 2626 1333 514 497 135 90 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 2294 748 Size: 1546 4011 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4671 4855 1305 Size: 3366 184 2007 Fragments arranged by size: 3366 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3735 Fragments arranged by size: 3735 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2304 3515 3874 4033 518 Size: 648 864 274 1211 359 159 2042 Fragments arranged by size: 2042 1211 864 648 359 274 159 MspA1I CmG'CkG Cuts at: 1606 2014 2093 2130 2500 2973 3650 4798 5043 Size: 408 79 37 370 473 677 1148 245 Cuts at: 5043 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 473 408 370 245 79 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4066 Fragments arranged by size: 4066 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2915 4460 1132 Size: 97 1686 1545 2229 Fragments arranged by size: 2229 1686 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PfoI T'CCnGG_A Cuts at: 2486 2486 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PmlI CAC'GTG Cuts at: 2346 2346 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2546 2546 Size: 5557 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PspOMI G'GGCC_C Cuts at: 2056 2056 Size: 5557 PspXI vC'TCGA_Gb Cuts at: 2507 2507 Size: 5557 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2438 2470 2438 Size: 32 5525 PstI C_TGCA'G Cuts at: 830 2092 2727 2856 2909 830 Size: 1262 635 129 53 3478 Fragments arranged by size: 3478 1262 635 129 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 PvuII CAG'CTG Cuts at: 2093 2130 2500 2093 Size: 37 370 5150 Fragments arranged by size: 5150 370 37 SacI G_AGCT'C Cuts at: 2688 2688 Size: 5557 SacII CC_GC'GG Cuts at: 2015 2015 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SanDI GG'GwC_CC Cuts at: 2546 2546 Size: 5557 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2088 2723 2852 2905 4040 4721 4912 Size: 219 1043 635 129 53 1135 681 191 Cuts at: 4912 826 Size: 1471 Fragments arranged by size: 1471 1135 1043 681 635 219 191 129 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2061 2884 1872 Size: 189 823 4545 Fragments arranged by size: 4545 823 189 SmlI C'TyrA_G Cuts at: 820 1017 1943 2507 2579 3661 4562 4824 820 Size: 197 926 564 72 1082 901 262 1553 Fragments arranged by size: 1553 1082 926 901 564 262 197 72 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 2996 513 Size: 552 939 108 884 3074 Fragments arranged by size: 3074 939 884 552 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3584 3601 3754 3939 4384 325 Size: 1255 2004 17 153 185 445 1498 Fragments arranged by size: 2004 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 5515 96 Size: 274 80 33 51 1443 1826 1712 138 Fragments arranged by size: 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5517 5549 5517 Size: 32 5525 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2299 2299 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 2507 1943 Size: 564 4993 XmaI C'CCGG_G Cuts at: 1870 2059 2882 1870 Size: 189 823 4545 Fragments arranged by size: 4545 823 189 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI RsrII SapI SexAI SfiI SfoI SrfI SwaI