Restriction map for
pFN21AE3275
(Linear) (Six-base) MAPSORT of: KIEE3275.seq Check: 4742 from: 1 to: 2064
KIEE3275.orf
With 182 enzymes: SgfI *
July 27, 2017 10:26 ..
AccI GT'mk_AC
Cuts at: 0 315 941 1382 2064
Size: 315 626 441 682
Fragments arranged by size:
682 626 441 315
Acc65I G'GTAC_C
Cuts at: 0 1054 2064
Size: 1054 1010
AclI AA'CG_TT
Cuts at: 0 536 2064
Size: 536 1528
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1282 2064
Size: 1270 12 782
Fragments arranged by size:
1270 782 12
AflIII A'CryG_T
Cuts at: 0 390 2064
Size: 390 1674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 675 2064
Size: 675 1389
AlwNI CAG_nnn'CTG
Cuts at: 0 637 2014 2064
Size: 637 1377 50
Fragments arranged by size:
1377 637 50
ApoI r'AATT_y
Cuts at: 0 320 455 490 1794 2064
Size: 320 135 35 1304 270
Fragments arranged by size:
1304 320 270 135 35
AseI AT'TA_AT
Cuts at: 0 227 2064
Size: 227 1837
AvaI C'yCGr_G
Cuts at: 0 1075 1418 2064
Size: 1075 343 646
Fragments arranged by size:
1075 646 343
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1378 1411 2064
Size: 1378 33 653
Fragments arranged by size:
1378 653 33
BanI G'GyrC_C
Cuts at: 0 1054 2064
Size: 1054 1010
BanII G_rGCy'C
Cuts at: 0 110 2024 2064
Size: 110 1914 40
Fragments arranged by size:
1914 110 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 302 2064
Size: 302 1762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 75 109 2064
Size: 75 34 1955
Fragments arranged by size:
1955 75 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 124 1467 2064
Size: 124 1343 597
Fragments arranged by size:
1343 597 124
BfrBI ATG'CAT
Cuts at: 0 1856 2064
Size: 1856 208
BglII A'GATC_T
Cuts at: 0 1002 2064
Size: 1002 1062
Bme1580I G_kGCm'C
Cuts at: 0 1983 2064
Size: 1983 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 104 1711 2064
Size: 104 1607 353
Fragments arranged by size:
1607 353 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 2064
Size: 31 2033
BsaAI yAC'GTr
Cuts at: 0 393 2064
Size: 393 1671
BsaWI w'CCGG_w
Cuts at: 0 116 2064
Size: 116 1948
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 868 898 1108 1138 1236 1266 2064
Size: 868 30 210 30 98 30 798
Fragments arranged by size:
868 798 210 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 611 1787 2064
Size: 611 1176 277
Fragments arranged by size:
1176 611 277
BseYI C'CCAG_C
Cuts at: 0 1308 1961 2064
Size: 1308 653 103
Fragments arranged by size:
1308 653 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1942 2064
Size: 1942 122
BsiEI CG_ry'CG
Cuts at: 0 74 2064
Size: 74 1990
BsiHKAI G_wGCw'C
Cuts at: 0 1349 2024 2064
Size: 1349 675 40
Fragments arranged by size:
1349 675 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1286 2064
Size: 1286 778
Bsp1286I G_dGCh'C
Cuts at: 0 110 1349 1983 2024 2064
Size: 110 1239 634 41 40
Fragments arranged by size:
1239 634 110 41 40
BspEI T'CCGG_A
Cuts at: 0 116 2064
Size: 116 1948
BspHI T'CATG_A
Cuts at: 0 338 2064
Size: 338 1726
BsrDI GCAATG_nn'
Cuts at: 0 659 1903 2064
Size: 659 1244 161
Fragments arranged by size:
1244 659 161
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1588 2064
Size: 1588 476
BstYI r'GATC_y
Cuts at: 0 813 1002 2064
Size: 813 189 1062
Fragments arranged by size:
1062 813 189
Bsu36I CC'TnA_GG
Cuts at: 0 242 2064
Size: 242 1822
BtgI C'CryG_G
Cuts at: 0 47 1059 2064
Size: 47 1012 1005
Fragments arranged by size:
1012 1005 47
BtsI GCAGTG_nn'
Cuts at: 0 1116 1930 2064
Size: 1116 814 134
Fragments arranged by size:
1116 814 134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1388 1423 2064
Size: 1388 35 641
Fragments arranged by size:
1388 641 35
DraI TTT'AAA
Cuts at: 0 204 2064
Size: 204 1860
DraIII CAC_nnn'GTG
Cuts at: 0 861 2064
Size: 861 1203
EaeI y'GGCC_r
Cuts at: 0 23 44 71 1641 2064
Size: 23 21 27 1570 423
Fragments arranged by size:
1570 423 27 23 21
EagI C'GGCC_G
Cuts at: 0 71 2064
Size: 71 1993
EarI CTCTTCn'nnn_
Cuts at: 0 49 254 667 1463 1612 2064
Size: 49 205 413 796 149 452
Fragments arranged by size:
796 452 413 205 149 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2064
Size: 19 2045
EcoICRI GAG'CTC
Cuts at: 0 2022 2064
Size: 2022 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1270 1282 2064
Size: 31 1239 12 782
Fragments arranged by size:
1239 782 31 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1273 2006 2064
Size: 1273 733 58
Fragments arranged by size:
1273 733 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 2064
Size: 319 1745
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 414 446 1108 1140 1234 1266
Size: 162 32 220 32 662 32 94 32
Cuts at: 1266 1441 1473 1768 1800 2064
Size: 175 32 295 32 264
Fragments arranged by size:
662 295 264 220 175 162 94 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 316 1368 2064
Size: 316 1052 696
Fragments arranged by size:
1052 696 316
KpnI G_GTAC'C
Cuts at: 0 1058 2064
Size: 1058 1006
MscI TGG'CCA
Cuts at: 0 46 1643 2064
Size: 46 1597 421
Fragments arranged by size:
1597 421 46
MslI CAynn'nnrTG
Cuts at: 0 1046 2064
Size: 1046 1018
MspA1I CmG'CkG
Cuts at: 0 1965 2064
Size: 1965 99
NcoI C'CATG_G
Cuts at: 0 47 1059 2064
Size: 47 1012 1005
Fragments arranged by size:
1012 1005 47
NsiI A_TGCA'T
Cuts at: 0 1858 2064
Size: 1858 206
NspI r_CATG'y
Cuts at: 0 924 1140 1381 1695 2064
Size: 924 216 241 314 369
Fragments arranged by size:
924 369 314 241 216
PasI CC'CwG_GG
Cuts at: 0 1128 2064
Size: 1128 936
PfoI T'CCnGG_A
Cuts at: 0 809 2064
Size: 809 1255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1802 1834 2064
Size: 1802 32 230
Fragments arranged by size:
1802 230 32
PstI C_TGCA'G
Cuts at: 0 1088 2064
Size: 1088 976
SacI G_AGCT'C
Cuts at: 0 2024 2064
Size: 2024 40
SalI G'TCGA_C
Cuts at: 0 314 2064
Size: 314 1750
SapI GCTCTTCn'nnn_
Cuts at: 0 49 2064
Size: 49 2015
SfcI C'TryA_G
Cuts at: 0 383 942 1084 2064
Size: 383 559 142 980
Fragments arranged by size:
980 559 383 142
SmaI CCC'GGG
Cuts at: 0 1420 2064
Size: 1420 644
SphI G_CATG'C
Cuts at: 0 1140 2064
Size: 1140 924
SspI AAT'ATT
Cuts at: 0 966 2064
Size: 966 1098
StyI C'CwwG_G
Cuts at: 0 47 1059 2064
Size: 47 1012 1005
Fragments arranged by size:
1012 1005 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1223 1994 2064
Size: 1223 771 70
Fragments arranged by size:
1223 771 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1236 1268 2064
Size: 1236 32 796
Fragments arranged by size:
1236 796 32
XmaI C'CCGG_G
Cuts at: 0 1418 2064
Size: 1418 646
XmnI GAAnn'nnTTC
Cuts at: 0 467 568 902 1814 2064
Size: 467 101 334 912 250
Fragments arranged by size:
912 467 334 250 101
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApoI
AseI AvaI BaeI BanI BanII BbsI BcgI BciVI
BfrBI BglII Bme1580I BmrI BpmI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BsrDI BstAPI BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI FalI Hin4I HincII KpnI MscI MslI MspA1I
NcoI NsiI NspI PasI PfoI PpiI PstI SacI
SalI SapI SfcI SmaI SphI SspI StyI TaqII
TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI
BclI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsiWI BsmI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI
ClaI DrdI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PciI
PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI StuI SwaI TatI Tth111I XbaI XcmI XhoI
ZraI