################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MscI MslI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 60 TCCGGA T^CCGGA AfaI 36 GTAC GT^AC AluI 405 AGCT AG^CT AlwI 354 GATCC GGATC(4/5) ApaI 126 GGGCCC GGGCC^C AvaII 8 GGACC G^G[AT]CC BbsI 133 GAAGAC GAAGAC(2/6) BbvI 314 GCAGC GCAGC(8/12) BclI 119 TGATCA T^GATCA BcnI 441 CCGGG CC^[GC]GG BpuAI 133 GAAGAC GAAGAC(2/6) BsaJI 299 CCATGG C^C[ACGT][ACGT]GG BsaWI 60 TCCGGA [AT]^CCGG[AT] BseAI 60 TCCGGA T^CCGGA BsgI 337 GTGCAG GTGCAG(16/14) BsmAI 483 GTCTC GTCTC(1/5) BspEI 60 TCCGGA T^CCGGA BspMI 139 ACCTGC ACCTGC(4/8) BsrBI 322 GAGCGG CCGCTC(-3/-3) BstNI 286 CCAGG CC^[AT]GG BstYI 353 AGATCC [GA]^GATC[CT] DdeI 172 CTGAG C^T[ACGT]AG DraII 78 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 299 CCATGG C^C[GA][CT]GG EaeI 29 TGGCCG [CT]^GGCC[GA] EarI 91 GAAGAG CTCTTC(1/4) EcoNI 58 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 78 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRII 286 CCAGG ^CC[AT]GG EcoT14I 299 CCATGG C^C[AT][AT]GG FauI 4 GCGGG CCCGC(4/6) FbaI 119 TGATCA T^GATCA Fnu4HI 314 GCAGC GC^[ACGT]GC HgaI 418 GCGTC GACGC(5/10) HphI 369 GGTGA GGTGA(8/7) ItaI 314 GCAGC GC^[ACGT]GC Ksp632I 91 GAAGAG CTCTTC(1/4) MaeII 410 ACGT A^CGT MflI 353 AGATCC [GA]^GATC[CT] MroI 60 TCCGGA T^CCGGA MvaI 286 CCAGG CC^[AT]GG NciI 441 CCGGG CC^[GC]GG NcoI 299 CCATGG C^CATGG NspI 374 GCATGC [GA]CATG^[CT] PflMI 422 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 36 GTAC GT^AC SfaNI 415 GATGC GCATC(5/9) SmlI 16 CTCAAG C^T[CT][GA]AG SphI 374 GCATGC GCATG^C StyI 299 CCATGG C^C[AT][AT]GG TfiI 54 GAATC G^A[AT]TC TseI 314 GCAGC G^C[AT]GC TspRI 50 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 422 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 353 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BanII 126 GGGCCC G[GA]GC[CT]^C 385 GAGCCC BmyI 126 GGGCCC G[AGT]GC[ACT]^C 385 GAGCCC Bsp1286I 126 GGGCCC G[AGT]GC[ACT]^C 385 GAGCCC BssKI 286 CCAGG ^CC[ACGT]GG 441 CCGGG HapII 61 CCGG C^CGG 441 CCGG HpaII 61 CCGG C^CGG 441 CCGG MseI 46 TTAA T^TAA 196 TTAA MspI 61 CCGG C^CGG 441 CCGG PleI 260 GAGTC GAGTC(4/5) 330 GAGTC ScrFI 286 CCAGG CC^[ACGT]GG 441 CCGGG Tru9I 46 TTAA T^TAA 196 TTAA Tsp509I 44 AATT ^AATT 69 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AccII AciI BsiYI BslI BsmFI BstF5I BstUI Cac8I CfoI Cfr13I DpnII FokI HaeIII HhaI HinP1I HinfI MboI MboII MnlI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I