################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 74 GGTACC G^GTACC AlwI 669 GATCC GGATC(4/5) Asp718I 74 GGTACC G^GTACC AspHI 88 GAGCTC G[AT]GC[AT]^C AvaII 406 GGACC G^G[AT]CC BanI 74 GGTACC G^G[CT][GA]CC BanII 88 GAGCTC G[GA]GC[CT]^C BbsI 43 GTCTTC GAAGAC(2/6) BfaI 557 CTAG C^TAG BmyI 88 GAGCTC G[AGT]GC[ACT]^C BpmI 164 CTCCAG CTGGAG(16/14) BpuAI 43 GTCTTC GAAGAC(2/6) BsiHKAI 88 GAGCTC G[AT]GC[AT]^C BsmFI 169 GTCCC GGGAC(10/14) BsmI 504 GAATGC GAATGC(1/-1) Bsp1286I 88 GAGCTC G[AGT]GC[ACT]^C BspMI 77 ACCTGC ACCTGC(4/8) BsrDI 330 CATTGC GCAATG(2/0) BssKI 62 CCTGG ^CC[ACGT]GG BstEII 459 GGTGACC G^GT[ACGT]ACC BstF5I 129 CATCC GGATG(2/0) BstNI 62 CCTGG CC^[AT]GG BstPI 459 GGTGACC G^GT[ACGT]ACC BstYI 668 AGATCC [GA]^GATC[CT] CfoI 3 GCGC GCG^C Cfr13I 406 GGACC G^G[ACGT]CC EarI 451 CTCTTC CTCTTC(1/4) EcoNI 21 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 459 GGTGACC G^GT[ACGT]ACC EcoRII 62 CCTGG ^CC[AT]GG FokI 129 CATCC GGATG(9/13) HhaI 3 GCGC GCG^C HinP1I 3 GCGC G^CGC HincII 340 GTCAAC GT[CT]^[GA]AC HindII 340 GTCAAC GT[CT]^[GA]AC HinfI 198 GACTC G^A[ACGT]TC HphI 459 GGTGA GGTGA(8/7) KpnI 74 GGTACC GGTAC^C Ksp632I 451 CTCTTC CTCTTC(1/4) MaeI 557 CTAG C^TAG MflI 668 AGATCC [GA]^GATC[CT] MslI 625 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 62 CCTGG CC^[AT]GG NlaIV 74 GGTACC GG[ACGT]^[ACGT]CC PflMI 7 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 198 GACTC GAGTC(4/5) PstI 662 CTGCAG CTGCA^G PvuII 301 CAGCTG CAG^CTG SacI 88 GAGCTC GAGCT^C SapI 450 GCTCTTC GCTCTTC(1/4) Sau96I 406 GGACC G^G[ACGT]CC ScrFI 62 CCTGG CC^[ACGT]GG SmlI 25 CTCAAG C^T[CT][GA]AG StuI 148 AGGCCT AGG^CCT Van91I 7 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 668 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 132 CCGC CCGC(-3/-1) 239 GCGG AcsI 547 AAATTC [GA]^AATT[CT] 602 AAATTC AfaI 75 GTAC GT^AC 258 GTAC ApoI 547 AAATTC [GA]^AATT[CT] 602 AAATTC DdeI 235 CTCAG C^T[ACGT]AG 635 CTAAG DpnII 618 GATC ^GATC 669 GATC MboI 618 GATC ^GATC 669 GATC MboII 44 TCTTC GAAGA(8/7) 452 TCTTC MspA1I 237 CAGCGG C[AC]G^C[GT]G 301 CAGCTG NdeII 618 GATC ^GATC 669 GATC NlaIII 456 CATG CATG^ 543 CATG RsaI 75 GTAC GT^AC 258 GTAC Sau3AI 618 GATC ^GATC 669 GATC Tsp509I 548 AATT ^AATT 603 AATT TspRI 114 CAGTG CA[GC]TG[ACGT][ACGT]^ 193 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI BbvI BsiYI BslI BsrI Cac8I Fnu4HI HaeIII ItaI MaeIII MnlI MwoI SfaNI SfcI TseI Tsp45I