(Linear) (Six-base) MAPSORT of: KIEE3075.seq  Check: 1920  from: 1  to: 705



KIEE3075.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:10  ..

Acc65I G'GTAC_C

Cuts at:      0      74     705 
   Size:         74     631
 
ApoI r'AATT_y

Cuts at:      0     547     602     705 
   Size:        547      55     103

  Fragments arranged by size: 

                 547     103      55
 
BanI G'GyrC_C

Cuts at:      0      74     705 
   Size:         74     631
 
BanII G_rGCy'C

Cuts at:      0      92     705 
   Size:         92     613
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      36     705 
   Size:         36     669
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     362     705 
   Size:        362     343
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     452     705 
   Size:        452     253
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     705 
   Size:        149     556
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     705 
   Size:         10     695
 
BseYI C'CCAG_C

Cuts at:      0     303     560     705 
   Size:        303     257     145

  Fragments arranged by size: 

                 303     257     145
 
BsiHKAI G_wGCw'C

Cuts at:      0      92     705 
   Size:         92     613
 
BsmI GAATG_Cn'

Cuts at:      0     510     705 
   Size:        510     195
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     705 
   Size:         92     613
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      86     705 
   Size:         86     619
 
BsrDI GCAATG_nn'

Cuts at:      0     329     705 
   Size:        329     376
 
BstEII G'GTnAC_C

Cuts at:      0     459     705 
   Size:        459     246
 
BstYI r'GATC_y

Cuts at:      0     668     705 
   Size:        668      37
 
EarI CTCTTCn'nnn_

Cuts at:      0     457     705 
   Size:        457     248
 
EcoICRI GAG'CTC

Cuts at:      0      90     705 
   Size:         90     615
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     705 
   Size:        149     556
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25     705 
   Size:         25     680
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     602     634     705 
   Size:        602      32      71

  Fragments arranged by size: 

                 602      71      32
 
HincII GTy'rAC

Cuts at:      0     342     705 
   Size:        342     363
 
KpnI G_GTAC'C

Cuts at:      0      78     705 
   Size:         78     627
 
MslI CAynn'nnrTG

Cuts at:      0     629     705 
   Size:        629      76
 
MspA1I CmG'CkG

Cuts at:      0     239     303     705 
   Size:        239      64     402

  Fragments arranged by size: 

                 402     239      64
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      13     705 
   Size:         13     692
 
PstI C_TGCA'G

Cuts at:      0     666     705 
   Size:        666      39
 
PvuII CAG'CTG

Cuts at:      0     303     705 
   Size:        303     402
 
SacI G_AGCT'C

Cuts at:      0      92     705 
   Size:         92     613
 
SapI GCTCTTCn'nnn_

Cuts at:      0     457     705 
   Size:        457     248
 
SfcI C'TryA_G

Cuts at:      0     123     225     662     705 
   Size:        123     102     437      43

  Fragments arranged by size: 

                 437     123     102      43
 
SmlI C'TyrA_G

Cuts at:      0      25     705 
   Size:         25     680
 
StuI AGG'CCT

Cuts at:      0     150     705 
   Size:        150     555
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     423     705 
   Size:        423     282
 

 Enzymes that do cut:

   Acc65I     ApoI     BanI    BanII     BbsI     BmrI     BplI     BpmI
    BpuEI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI   BstEII
    BstYI     EarI  EcoICRI  Eco57MI    EcoNI    Hin4I   HincII     KpnI
     MslI   MspA1I    PflMI     PstI    PvuII     SacI     SapI     SfcI
     SmlI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI