Restriction map for
pFN21AE3292
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AocI
Aor51HI ApaI AscI Asp718I AspI
AvrII BbeI BbrPI BbvCI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV FseI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcsI 1400 GAATTC [GA]^AATT[CT]
AhdI 1656 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 807 GTGCAC G^TGCAC
ApaLI 807 GTGCAC G^TGCAC
ApoI 1400 GAATTC [GA]^AATT[CT]
AseI 319 ATTAAT AT^TAAT
AsnI 319 ATTAAT AT^TAAT
Asp700I 281 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1656 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1039 CTCGGG C^[CT]CG[GA]G
AviII 795 TGCGCA TGC^GCA
BalI 1944 TGGCCA TGG^CCA
BamHI 1587 GGATCC G^GATCC
BcgI 1596 GCACCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 299 TGATCA T^GATCA
BcnI 1580 CCCGG CC^[GC]GG
BglI 1633 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1877 ACCGGA [AT]^CCGG[AT]
BsmBI 371 GAGACG CGTCTC(1/5)
BsoBI 1039 CTCGGG C^[CT]CG[GA]G
BspHI 626 TCATGA T^CATGA
BspMI 1140 ACCTGC ACCTGC(4/8)
BsrDI 1790 CATTGC GCAATG(2/0)
BstBI 689 TTCGAA TT^CGAA
BstYI 1587 GGATCC [GA]^GATC[CT]
CfoI 796 GCGC GCG^C
DrdI 1965 GACATCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1944 TGGCCA [CT]^GGCC[GA]
Eam1105I 1656 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2236 GAAGAG CTCTTC(1/4)
EcoRI 1400 GAATTC G^AATTC
FbaI 299 TGATCA T^GATCA
FspI 795 TGCGCA TGC^GCA
HhaI 796 GCGC GCG^C
HinP1I 796 GCGC G^CGC
Ksp632I 2236 GAAGAG CTCTTC(1/4)
MflI 1587 GGATCC [GA]^GATC[CT]
MluNI 1944 TGGCCA TGG^CCA
MscI 1944 TGGCCA TGG^CCA
NciI 1580 CCCGG CC^[GC]GG
NdeI 527 CATATG CA^TATG
NheI 2281 GCTAGC G^CTAGC
NspI 25 GCATGC [GA]CATG^[CT]
NspV 689 TTCGAA TT^CGAA
PshBI 319 ATTAAT AT^TAAT
PstI 1420 CTGCAG CTGCA^G
RcaI 626 TCATGA T^CATGA
SacI 622 GAGCTC GAGCT^C
SfuI 689 TTCGAA TT^CGAA
SmlI 619 CTTGAG C^T[CT][GA]AG
SphI 25 GCATGC GCATG^C
StuI 2033 AGGCCT AGG^CCT
XcmI 1195 CCAGATGGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1587 GGATCC [GA]^GATC[CT]
XmnI 281 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1894 GTCTAC GT^[AC][GT]AC
2110 GTCTAC
AspHI 622 GAGCTC G[AT]GC[AT]^C
807 GTGCAC
BanI 1087 GGTGCC G^G[CT][GA]CC
1516 GGCACC
BanII 622 GAGCTC G[GA]GC[CT]^C
1577 GAGCCC
BfaI 380 CTAG C^TAG
2282 CTAG
BsaI 768 GGTCTC GGTCTC(1/5)
983 GGTCTC
BsiEI 1334 CGGTCG CG[GA][CT]^CG
1582 CGGTCG
BsiHKAI 622 GAGCTC G[AT]GC[AT]^C
807 GTGCAC
BsmI 1004 GAATGC GAATGC(1/-1)
1785 GAATGC
BstEII 1873 GGTAACC G^GT[ACGT]ACC
2017 GGTAACC
BstPI 1873 GGTAACC G^GT[ACGT]ACC
2017 GGTAACC
DraII 1296 AGGACCT [GA]G^G[ACGT]CC[CT]
2178 AGGTCCT
EcoO109I 1296 AGGACCT [GA]G^G[ACGT]CC[CT]
2178 AGGTCCT
EcoO65I 1873 GGTAACC G^GT[ACGT]ACC
2017 GGTAACC
EcoT22I 1 ATGCAT ATGCA^T
1787 ATGCAT
FauI 1489 CCCGC CCCGC(4/6)
2123 CCCGC
HapII 1581 CCGG C^CGG
1878 CCGG
HgaI 1593 GACGC GACGC(5/10)
1674 GCGTC
HincII 292 GTTGAC GT[CT]^[GA]AC
916 GTTGAC
HindII 292 GTTGAC GT[CT]^[GA]AC
916 GTTGAC
HpaII 1581 CCGG C^CGG
1878 CCGG
MaeI 380 CTAG C^TAG
2282 CTAG
MaeII 1311 ACGT A^CGT
2096 ACGT
MspA1I 1445 CAGCTG C[AC]G^C[GT]G
1628 CAGCTG
MspI 1581 CCGG C^CGG
1878 CCGG
NsiI 1 ATGCAT ATGCA^T
1787 ATGCAT
PpuMI 1296 AGGACCT [GA]G^G[AT]CC[CT]
2178 AGGTCCT
PvuII 1445 CAGCTG CAG^CTG
1628 CAGCTG
SexAI 203 ACCAGGT A^CC[AT]GGT
1299 ACCTGGT
SfcI 1420 CTGCAG C^T[GA][CT]AG
2276 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbsI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I