(Circular) (Six-base) MAPSORT of: pFN21AE3292.seq Check: 4564 from: 1 to: 6994 pFN21AE3292 6994 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3154 3154 Size: 6994 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4804 278 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3900 4116 4337 1937 Size: 1963 216 221 4594 Fragments arranged by size: 4594 1963 221 216 Acc65I G'GTAC_C Cuts at: 4315 4315 Size: 6994 AclI AA'CG_TT Cuts at: 1563 5121 5494 1563 Size: 3558 373 3063 Fragments arranged by size: 3558 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2010 2072 3738 4224 5057 6441 6709 1632 Size: 378 62 1666 486 833 1384 268 1917 Fragments arranged by size: 1917 1666 1384 833 486 378 268 62 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6797 AflIII A'CryG_T Cuts at: 1203 5893 6870 1203 Size: 4690 977 1327 Fragments arranged by size: 4690 1327 977 AgeI A'CCGG_T Cuts at: 5825 5825 Size: 6994 AhdI GACnn_n'nnGTC Cuts at: 3666 5723 3666 Size: 2057 4937 AleI CACnn'nnGTG Cuts at: 1166 3618 1166 Size: 2452 4542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2837 3708 1184 Size: 1653 871 4470 Fragments arranged by size: 4470 1653 871 AlwNI CAG_nnn'CTG Cuts at: 2273 3716 4061 4187 4737 6309 2273 Size: 1443 345 126 550 1572 2958 Fragments arranged by size: 2958 1572 1443 550 345 126 ApaLI G'TGCA_C Cuts at: 2812 5051 6207 2812 Size: 2239 1156 3599 Fragments arranged by size: 3599 2239 1156 ApoI r'AATT_y Cuts at: 1493 3405 4303 4529 4681 6790 1493 Size: 1912 898 226 152 2109 1697 Fragments arranged by size: 2109 1912 1697 898 226 152 AseI AT'TA_AT Cuts at: 160 1032 2325 5548 160 Size: 872 1293 3223 1606 Fragments arranged by size: 3223 1606 1293 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 AvaI C'yCGr_G Cuts at: 1870 1943 3044 4319 1870 Size: 73 1101 1275 4545 Fragments arranged by size: 4545 1275 1101 73 BamHI G'GATC_C Cuts at: 3592 4324 4691 3592 Size: 732 367 5895 Fragments arranged by size: 5895 732 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3092 3521 4315 5770 618 Size: 325 217 627 1305 429 794 1455 1842 Fragments arranged by size: 1842 1455 1305 794 627 429 325 217 BanII G_rGCy'C Cuts at: 1391 2631 3586 4313 1391 Size: 1240 955 727 4072 Fragments arranged by size: 4072 1240 955 727 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2362 3230 3507 3972 928 Size: 971 463 868 277 465 3950 Fragments arranged by size: 3950 971 868 465 463 277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3590 3624 5185 5219 1903 Size: 34 50 34 1569 34 1561 34 3678 Fragments arranged by size: 3678 1569 1561 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4886 6096 4886 Size: 1210 5784 BclI T'GATC_A Cuts at: 1548 2304 1548 Size: 756 6238 BfrBI ATG'CAT Cuts at: 2008 3794 2008 Size: 1786 5208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3644 5605 136 Size: 107 122 71 631 605 1972 1961 1525 Fragments arranged by size: 1972 1961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6989 6989 Size: 6994 BlpI GC'TnA_GC Cuts at: 4411 4411 Size: 6994 Bme1580I G_kGCm'C Cuts at: 1790 2816 3524 3627 5055 6211 1790 Size: 1026 708 103 1428 1156 2573 Fragments arranged by size: 2573 1428 1156 1026 708 103 BmgBI CAC'GTC Cuts at: 1318 3317 1318 Size: 1999 4995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3014 4049 4725 Size: 416 53 83 399 24 1597 1035 676 Cuts at: 4725 5683 442 Size: 958 1753 Fragments arranged by size: 1753 1597 1035 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4290 1056 Size: 3234 3760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2823 2976 3093 3514 5654 1239 Size: 1584 153 117 421 2140 2579 Fragments arranged by size: 2579 2140 1584 421 153 117 Bpu10I CC'TnA_GC Cuts at: 3547 3547 Size: 6994 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 5119 5984 6282 2645 Size: 2474 865 298 3357 Fragments arranged by size: 3357 2474 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2779 2994 5657 882 Size: 617 1280 215 2663 2219 Fragments arranged by size: 2663 2219 1280 617 215 BsaAI yAC'GTr Cuts at: 493 1204 4785 493 Size: 711 3581 2702 Fragments arranged by size: 3581 2702 711 BsaBI GATnn'nnATC Cuts at: 6988 6988 Size: 6994 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4801 5183 5818 275 Size: 53 83 186 936 3268 382 635 1451 Fragments arranged by size: 3268 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3882 5427 5825 6099 6246 6657 3882 Size: 1545 398 274 147 411 4219 Fragments arranged by size: 4219 1545 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3706 3736 4251 4281 1178 Size: 30 165 30 2303 30 515 30 3891 Fragments arranged by size: 3891 2303 515 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2031 3687 3913 4003 1186 Size: 845 1656 226 90 4177 Fragments arranged by size: 4177 1656 845 226 90 BseYI C'CCAG_C Cuts at: 2266 3320 6197 6693 2266 Size: 1054 2877 496 2567 Fragments arranged by size: 2877 2567 1054 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2802 3662 4173 1163 Size: 573 1066 860 511 3984 Fragments arranged by size: 3984 1066 860 573 511 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3342 3590 4444 5205 6233 1540 Size: 275 186 1341 248 854 761 1028 2301 Fragments arranged by size: 2301 1341 1028 854 761 275 248 186 BsiHKAI G_wGCw'C Cuts at: 2631 2816 5055 5140 6211 2631 Size: 185 2239 85 1071 3414 Fragments arranged by size: 3414 2239 1071 185 85 BsmI GAATG_Cn' Cuts at: 3015 3796 4505 4598 3015 Size: 781 709 93 5411 Fragments arranged by size: 5411 781 709 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2370 2370 Size: 6994 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2631 2816 3524 3586 3627 4313 5055 Size: 399 841 185 708 62 41 686 742 Cuts at: 5055 5140 6211 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 841 742 708 686 399 185 85 62 41 BspEI T'CCGG_A Cuts at: 6657 6657 Size: 6994 BspHI T'CATG_A Cuts at: 2631 4883 6613 2631 Size: 2252 1730 3012 Fragments arranged by size: 3012 2252 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3154 4349 844 Size: 2310 1195 3489 Fragments arranged by size: 3489 2310 1195 BsrBI CCG'CTC Cuts at: 1394 1823 4881 1394 Size: 429 3058 3507 Fragments arranged by size: 3507 3058 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3794 5489 5663 65 Size: 1183 2546 1695 174 1396 Fragments arranged by size: 2546 1695 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5638 5825 1700 Size: 110 3828 187 2869 Fragments arranged by size: 3828 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6994 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6994 BssSI C'ACGA_G Cuts at: 5054 6066 5054 Size: 1012 5982 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6994 BstBI TT'CG_AA Cuts at: 2695 5798 2695 Size: 3103 3891 BstEII G'GTnAC_C Cuts at: 1184 3878 4022 1184 Size: 2694 144 4156 Fragments arranged by size: 4156 2694 144 BstXI CCAn_nnnn'nTGG Cuts at: 2081 3860 4139 2081 Size: 1779 279 4936 Fragments arranged by size: 4936 1779 279 BstYI r'GATC_y Cuts at: 1972 3592 4324 4691 5076 5093 6545 6631 6643 Size: 1620 732 367 385 17 1452 86 12 Cuts at: 6643 6989 1972 Size: 346 1977 Fragments arranged by size: 1977 1620 1452 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3498 3648 3808 3859 513 Size: 552 2433 150 160 51 3648 Fragments arranged by size: 3648 2433 552 160 150 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6255 Fragments arranged by size: 6255 683 56 BtsI GCAGTG_nn' Cuts at: 2814 2819 2911 4510 5304 5324 2814 Size: 5 92 1599 794 20 4484 Fragments arranged by size: 4484 1599 794 92 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6994 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6959 DraI TTT'AAA Cuts at: 4298 4298 Size: 6994 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3976 6001 809 Size: 507 2660 2025 1802 Fragments arranged by size: 2660 2025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3949 4441 5330 Size: 54 1425 50 275 18 2119 492 889 Cuts at: 5330 6959 8 Size: 1629 43 Fragments arranged by size: 2119 1629 1425 889 492 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4441 1537 Size: 275 2629 4090 Fragments arranged by size: 4090 2629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4236 4924 1353 Size: 2883 688 3423 Fragments arranged by size: 3423 2883 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 5581 5955 6101 387 Size: 1064 138 668 3324 374 146 1280 Fragments arranged by size: 3324 1280 1064 668 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 2629 Size: 6994 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2072 2823 2976 3093 3514 3738 Size: 393 378 62 751 153 117 421 224 Cuts at: 3738 4224 5057 5654 6441 6709 1239 Size: 486 833 597 787 268 1524 Fragments arranged by size: 1524 833 787 751 597 486 421 393 378 268 224 153 117 62 EcoO109I rG'GnC_Cy Cuts at: 3302 4184 3302 Size: 882 6112 EcoRI G'AATT_C Cuts at: 3405 4303 4681 3405 Size: 898 378 5718 Fragments arranged by size: 5718 898 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2636 2636 Size: 6994 FspI TGC'GCA Cuts at: 2802 5500 2802 Size: 2698 4296 HaeII r_GCGC'y Cuts at: 1678 6141 1678 Size: 4463 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3023 3055 3652 3684 5635 Size: 32 135 32 1451 32 597 32 1951 Cuts at: 5635 5667 5709 5741 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1951 1451 597 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2299 2923 4338 4584 669 Size: 988 281 361 624 1415 246 3079 Fragments arranged by size: 3079 1415 988 624 361 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6994 HpaI GTT'AAC Cuts at: 4584 4584 Size: 6994 KpnI G_GTAC'C Cuts at: 4319 4319 Size: 6994 MfeI C'AATT_G Cuts at: 4593 4593 Size: 6994 MluI A'CGCG_T Cuts at: 6870 6870 Size: 6994 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3570 3620 3935 4181 6108 6292 1305 Size: 2265 50 315 246 1927 184 2007 Fragments arranged by size: 2265 2007 1927 315 246 184 50 MscI TGG'CCA Cuts at: 10 64 1489 1832 3951 10 Size: 54 1425 343 2119 3053 Fragments arranged by size: 3053 2119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2325 2651 3032 3618 3807 3858 4952 Size: 648 1159 326 381 586 189 51 1094 Cuts at: 4952 5311 5470 518 Size: 359 159 2042 Fragments arranged by size: 2042 1159 1094 648 586 381 359 326 189 159 51 MspA1I CmG'CkG Cuts at: 1606 3452 3635 4410 5087 6235 6480 1606 Size: 1846 183 775 677 1148 245 2120 Fragments arranged by size: 2120 1846 1148 775 677 245 183 NcoI C'CATG_G Cuts at: 513 1065 3498 3648 3808 3859 513 Size: 552 2433 150 160 51 3648 Fragments arranged by size: 3648 2433 552 160 150 51 NdeI CA'TA_TG Cuts at: 387 2533 387 Size: 2146 4848 NheI G'CTAG_C Cuts at: 1052 4286 1052 Size: 3234 3760 NotI GC'GGCC_GC Cuts at: 4441 4441 Size: 6994 NsiI A_TGCA'T Cuts at: 2010 3796 2010 Size: 1786 5208 NspI r_CATG'y Cuts at: 1132 1229 2034 4352 5897 1132 Size: 97 805 2318 1545 2229 Fragments arranged by size: 2318 2229 1545 805 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6994 PciI A'CATG_T Cuts at: 5893 5893 Size: 6994 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6994 PmeI GTTT'AAAC Cuts at: 4298 4298 Size: 6994 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5063 5095 6576 6608 5063 Size: 32 1481 32 5449 Fragments arranged by size: 5449 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3302 4184 3302 Size: 882 6112 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6994 PsiI TTA'TAA Cuts at: 4564 4564 Size: 6994 PstI C_TGCA'G Cuts at: 830 3429 4346 830 Size: 2599 917 3478 Fragments arranged by size: 3478 2599 917 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6994 PvuII CAG'CTG Cuts at: 3452 3635 3452 Size: 183 6811 SacI G_AGCT'C Cuts at: 2631 2631 Size: 6994 SalI G'TCGA_C Cuts at: 1936 4336 1936 Size: 2400 4594 SbfI CC_TGCA'GG Cuts at: 4346 4346 Size: 6994 ScaI AGT'ACT Cuts at: 5242 6954 5242 Size: 1712 5282 SexAI A'CCwGG_T Cuts at: 2208 3304 2208 Size: 1096 5898 SfcI C'TryA_G Cuts at: 826 1045 3425 4281 4342 5477 6158 6349 826 Size: 219 2380 856 61 1135 681 191 1471 Fragments arranged by size: 2380 1471 1135 856 681 219 191 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6994 SmaI CCC'GGG Cuts at: 1872 4321 1872 Size: 2449 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2624 5098 5999 6261 820 Size: 197 926 681 2474 901 262 1553 Fragments arranged by size: 2474 1553 926 901 681 262 197 SnaBI TAC'GTA Cuts at: 493 4785 493 Size: 4292 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6994 SphI G_CATG'C Cuts at: 1132 2034 4352 1132 Size: 902 2318 3774 Fragments arranged by size: 3774 2318 902 SspI AAT'ATT Cuts at: 5 52 4918 5 Size: 47 4866 2081 Fragments arranged by size: 4866 2081 47 StuI AGG'CCT Cuts at: 1517 4040 1517 Size: 2523 4471 StyI C'CwwG_G Cuts at: 513 1065 3498 3648 3808 3859 3873 3893 4027 Size: 552 2433 150 160 51 14 20 134 Cuts at: 4027 4071 4433 513 Size: 44 362 3074 Fragments arranged by size: 3074 2433 552 362 160 150 134 51 44 20 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2976 5021 5038 5191 5376 5821 325 Size: 1255 1396 2045 17 153 185 445 1498 Fragments arranged by size: 2045 1498 1396 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2121 3246 3533 Size: 274 80 33 51 1443 144 1125 287 Cuts at: 3533 5240 6952 96 Size: 1707 1712 138 Fragments arranged by size: 1712 1707 1443 1125 287 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2811 2843 3706 3738 4251 4283 6954 6986 2811 Size: 32 863 32 513 32 2671 32 2819 Fragments arranged by size: 2819 2671 863 513 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6994 XbaI T'CTAG_A Cuts at: 4330 4330 Size: 6994 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3207 3207 Size: 6994 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6994 XmaI C'CCGG_G Cuts at: 1870 4319 1870 Size: 2449 4545 XmnI GAAnn'nnTTC Cuts at: 2290 5123 2290 Size: 2833 4161 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4802 276 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI