Restriction map for
pFN21AE3292
(Linear) (Six-base) MAPSORT of: KIEE3292.seq Check: 1953 from: 1 to: 2289
KIEE3292.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1149 2289
Size: 1149 1140
AccI GT'mk_AC
Cuts at: 0 1895 2111 2289
Size: 1895 216 178
Fragments arranged by size:
1895 216 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 67 1733 2219 2289
Size: 5 62 1666 486 70
Fragments arranged by size:
1666 486 70 62 5
AhdI GACnn_n'nnGTC
Cuts at: 0 1661 2289
Size: 1661 628
AleI CACnn'nnGTG
Cuts at: 0 1613 2289
Size: 1613 676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 1703 2289
Size: 832 871 586
Fragments arranged by size:
871 832 586
AlwNI CAG_nnn'CTG
Cuts at: 0 268 1711 2056 2182 2289
Size: 268 1443 345 126 107
Fragments arranged by size:
1443 345 268 126 107
ApaLI G'TGCA_C
Cuts at: 0 807 2289
Size: 807 1482
ApoI r'AATT_y
Cuts at: 0 1400 2289
Size: 1400 889
AseI AT'TA_AT
Cuts at: 0 320 2289
Size: 320 1969
AvaI C'yCGr_G
Cuts at: 0 1039 2289
Size: 1039 1250
BamHI G'GATC_C
Cuts at: 0 1587 2289
Size: 1587 702
BanI G'GyrC_C
Cuts at: 0 1087 1516 2289
Size: 1087 429 773
Fragments arranged by size:
1087 773 429
BanII G_rGCy'C
Cuts at: 0 626 1581 2289
Size: 626 955 708
Fragments arranged by size:
955 708 626
BbsI GAAGACnn'nnnn_
Cuts at: 0 357 1225 1502 1967 2289
Size: 357 868 277 465 322
Fragments arranged by size:
868 465 357 322 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1585 1619 2289
Size: 1585 34 670
Fragments arranged by size:
1585 670 34
BclI T'GATC_A
Cuts at: 0 299 2289
Size: 299 1990
BfrBI ATG'CAT
Cuts at: 0 3 1789 2289
Size: 3 1786 500
Fragments arranged by size:
1786 500 3
BglI GCCn_nnn'nGGC
Cuts at: 0 1639 2289
Size: 1639 650
Bme1580I G_kGCm'C
Cuts at: 0 811 1519 1622 2289
Size: 811 708 103 667
Fragments arranged by size:
811 708 667 103
BmgBI CAC'GTC
Cuts at: 0 1312 2289
Size: 1312 977
BmrI ACTGGGnnnn_n'
Cuts at: 0 1009 2044 2289
Size: 1009 1035 245
Fragments arranged by size:
1035 1009 245
BmtI G_CTAG'C
Cuts at: 0 2285 2289
Size: 2285 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 971 1088 1509 2289
Size: 818 153 117 421 780
Fragments arranged by size:
818 780 421 153 117
Bpu10I CC'TnA_GC
Cuts at: 0 1542 2289
Size: 1542 747
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 2289
Size: 640 1649
BsaI GGTCTCn'nnnn_
Cuts at: 0 774 989 2289
Size: 774 215 1300
Fragments arranged by size:
1300 774 215
BsaWI w'CCGG_w
Cuts at: 0 1877 2289
Size: 1877 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1701 1731 2246 2276 2289
Size: 1701 30 515 30 13
Fragments arranged by size:
1701 515 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 1682 1908 1998 2289
Size: 26 1656 226 90 291
Fragments arranged by size:
1656 291 226 90 26
BseYI C'CCAG_C
Cuts at: 0 261 1315 2289
Size: 261 1054 974
Fragments arranged by size:
1054 974 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1657 2168 2289
Size: 797 860 511 121
Fragments arranged by size:
860 797 511 121
BsiEI CG_ry'CG
Cuts at: 0 1337 1585 2289
Size: 1337 248 704
Fragments arranged by size:
1337 704 248
BsiHKAI G_wGCw'C
Cuts at: 0 626 811 2289
Size: 626 185 1478
Fragments arranged by size:
1478 626 185
BsmI GAATG_Cn'
Cuts at: 0 1010 1791 2289
Size: 1010 781 498
Fragments arranged by size:
1010 781 498
BsmBI CGTCTCn'nnnn_
Cuts at: 0 365 2289
Size: 365 1924
Bsp1286I G_dGCh'C
Cuts at: 0 626 811 1519 1581 1622 2289
Size: 626 185 708 62 41 667
Fragments arranged by size:
708 667 626 185 62 41
BspHI T'CATG_A
Cuts at: 0 626 2289
Size: 626 1663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1149 2289
Size: 1149 1140
BsrDI GCAATG_nn'
Cuts at: 0 1789 2289
Size: 1789 500
BstBI TT'CG_AA
Cuts at: 0 690 2289
Size: 690 1599
BstEII G'GTnAC_C
Cuts at: 0 1873 2017 2289
Size: 1873 144 272
Fragments arranged by size:
1873 272 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 76 1855 2134 2289
Size: 76 1779 279 155
Fragments arranged by size:
1779 279 155 76
BstYI r'GATC_y
Cuts at: 0 1587 2289
Size: 1587 702
BtgI C'CryG_G
Cuts at: 0 1493 1643 1803 1854 2289
Size: 1493 150 160 51 435
Fragments arranged by size:
1493 435 160 150 51
BtsI GCAGTG_nn'
Cuts at: 0 809 814 906 2289
Size: 809 5 92 1383
Fragments arranged by size:
1383 809 92 5
DrdI GACnn_nn'nnGTC
Cuts at: 0 1971 2289
Size: 1971 318
EaeI y'GGCC_r
Cuts at: 0 1944 2289
Size: 1944 345
EarI CTCTTCn'nnn_
Cuts at: 0 2231 2289
Size: 2231 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 2289
Size: 252 2037
EcoICRI GAG'CTC
Cuts at: 0 624 2289
Size: 624 1665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 67 818 971 1088 1509 1733 2219
Size: 5 62 751 153 117 421 224 486
Cuts at: 2219 2289
Size: 70
Fragments arranged by size:
751 486 421 224 153 117 70 62
5
EcoO109I rG'GnC_Cy
Cuts at: 0 1297 2179 2289
Size: 1297 882 110
Fragments arranged by size:
1297 882 110
EcoRI G'AATT_C
Cuts at: 0 1400 2289
Size: 1400 889
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 631 2289
Size: 631 1658
FspI TGC'GCA
Cuts at: 0 797 2289
Size: 797 1492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1018 1050 1647 1679 2289
Size: 1018 32 597 32 610
Fragments arranged by size:
1018 610 597 32 32
HincII GTy'rAC
Cuts at: 0 294 918 2289
Size: 294 624 1371
Fragments arranged by size:
1371 624 294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1565 1615 1930 2176 2289
Size: 1565 50 315 246 113
Fragments arranged by size:
1565 315 246 113 50
MscI TGG'CCA
Cuts at: 0 1946 2289
Size: 1946 343
MslI CAynn'nnrTG
Cuts at: 0 320 646 1027 1613 1802 1853 2289
Size: 320 326 381 586 189 51 436
Fragments arranged by size:
586 436 381 326 320 189 51
MspA1I CmG'CkG
Cuts at: 0 1447 1630 2289
Size: 1447 183 659
Fragments arranged by size:
1447 659 183
NcoI C'CATG_G
Cuts at: 0 1493 1643 1803 1854 2289
Size: 1493 150 160 51 435
Fragments arranged by size:
1493 435 160 150 51
NdeI CA'TA_TG
Cuts at: 0 528 2289
Size: 528 1761
NheI G'CTAG_C
Cuts at: 0 2281 2289
Size: 2281 8
NsiI A_TGCA'T
Cuts at: 0 5 1791 2289
Size: 5 1786 498
Fragments arranged by size:
1786 498 5
NspI r_CATG'y
Cuts at: 0 29 2289
Size: 29 2260
PpuMI rG'GwC_Cy
Cuts at: 0 1297 2179 2289
Size: 1297 882 110
Fragments arranged by size:
1297 882 110
PstI C_TGCA'G
Cuts at: 0 1424 2289
Size: 1424 865
PvuII CAG'CTG
Cuts at: 0 1447 1630 2289
Size: 1447 183 659
Fragments arranged by size:
1447 659 183
SacI G_AGCT'C
Cuts at: 0 626 2289
Size: 626 1663
SexAI A'CCwGG_T
Cuts at: 0 203 1299 2289
Size: 203 1096 990
Fragments arranged by size:
1096 990 203
SfcI C'TryA_G
Cuts at: 0 1420 2276 2289
Size: 1420 856 13
Fragments arranged by size:
1420 856 13
SmlI C'TyrA_G
Cuts at: 0 619 2289
Size: 619 1670
SphI G_CATG'C
Cuts at: 0 29 2289
Size: 29 2260
StuI AGG'CCT
Cuts at: 0 2035 2289
Size: 2035 254
StyI C'CwwG_G
Cuts at: 0 1493 1643 1803 1854 1868 1888 2022 2066
Size: 1493 150 160 51 14 20 134 44
Cuts at: 2066 2289
Size: 223
Fragments arranged by size:
1493 223 160 150 134 51 44 20
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 971 2289
Size: 971 1318
TatI w'GTAC_w
Cuts at: 0 116 1241 1528 2289
Size: 116 1125 287 761
Fragments arranged by size:
1125 761 287 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 806 838 1701 1733 2246 2278 2289
Size: 806 32 863 32 513 32 11
Fragments arranged by size:
863 806 513 32 32 32 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1202 2289
Size: 1202 1087
XmnI GAAnn'nnTTC
Cuts at: 0 285 2289
Size: 285 2004
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AlwNI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbsI BcgI
BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BstBI BstEII BstXI BstYI BtgI BtsI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI
NheI NsiI NspI PpuMI PstI PvuII SacI SexAI
SfcI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AloI
ApaI AscI AsiSI AvrII BaeI BbeI BbvCI BciVI
BglII BlpI BplI BsaAI BsaBI BsaHI BsiWI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EcoNI EcoRV
FseI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI