(Circular) (Six-base) MAPSORT of: pFN21AB9635.seq Check: 4428 from: 1 to: 5674 pFN21AB9635 5674 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2258 3017 1937 Size: 321 759 4594 Fragments arranged by size: 4594 759 321 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3737 5121 5389 1632 Size: 2105 1384 268 1917 Fragments arranged by size: 2105 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 2922 4573 5550 1203 Size: 1719 1651 977 1327 Fragments arranged by size: 1719 1651 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2381 1184 Size: 1197 4477 AlwNI CAG_nnn'CTG Cuts at: 2329 2690 3417 4989 2329 Size: 361 727 1572 3014 Fragments arranged by size: 3014 1572 727 361 ApaLI G'TGCA_C Cuts at: 2654 3731 4887 2654 Size: 1077 1156 3441 Fragments arranged by size: 3441 1156 1077 ApoI r'AATT_y Cuts at: 1493 2983 3209 3361 5470 1493 Size: 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2134 2999 1870 Size: 73 191 865 4545 Fragments arranged by size: 4545 865 191 73 BamHI G'GATC_C Cuts at: 2738 3004 3371 2738 Size: 266 367 5041 Fragments arranged by size: 5041 367 266 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2902 2936 2995 4450 Size: 325 217 627 327 788 34 59 1455 Cuts at: 4450 618 Size: 1842 Fragments arranged by size: 1842 1455 788 627 327 325 217 59 34 BanII G_rGCy'C Cuts at: 1391 2055 2186 2993 1391 Size: 664 131 807 4072 Fragments arranged by size: 4072 807 664 131 BbeI G_GCGC'C Cuts at: 2940 2940 Size: 5674 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4703 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2687 4285 136 Size: 107 122 71 631 605 1015 1598 1525 Fragments arranged by size: 1598 1525 1015 631 605 122 107 71 BglII A'GATC_T Cuts at: 2529 5669 2529 Size: 3140 2534 BlpI GC'TnA_GC Cuts at: 2156 3091 2156 Size: 935 4739 Bme1580I G_kGCm'C Cuts at: 1790 2117 2658 3735 4891 1790 Size: 327 541 1077 1156 2573 Fragments arranged by size: 2573 1156 1077 541 327 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2118 2875 3405 Size: 416 53 83 399 24 701 757 530 Cuts at: 3405 4363 442 Size: 958 1753 Fragments arranged by size: 1753 958 757 701 530 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 4334 1239 Size: 1283 1812 2579 Fragments arranged by size: 2579 1812 1283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2321 2771 3799 4664 4962 2321 Size: 450 1028 865 298 3033 Fragments arranged by size: 3033 1028 865 450 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4337 882 Size: 617 2838 2219 Fragments arranged by size: 2838 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2923 3465 493 Size: 711 1719 542 2702 Fragments arranged by size: 2702 1719 711 542 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 2937 2967 3481 Size: 53 83 186 936 483 921 30 514 Cuts at: 3481 3863 4498 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 921 635 514 483 382 186 83 53 30 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5449 Fragments arranged by size: 5449 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 1186 Size: 1333 4341 BseYI C'CCAG_C Cuts at: 2818 4877 5373 2818 Size: 2059 496 3119 Fragments arranged by size: 3119 2059 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2757 2811 3124 3885 4913 1540 Size: 275 186 756 54 313 761 1028 2301 Fragments arranged by size: 2301 1028 761 756 313 275 186 54 BsiHKAI G_wGCw'C Cuts at: 2658 3735 3820 4891 2658 Size: 1077 85 1071 3441 Fragments arranged by size: 3441 1077 1071 85 BsiWI C'GTAC_G Cuts at: 2412 2412 Size: 5674 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 BsmBI CGTCTCn'nnnn_ Cuts at: 2339 2339 Size: 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2117 2186 2658 2993 3735 3820 Size: 399 265 62 69 472 335 742 85 Cuts at: 3820 4891 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 335 265 85 69 62 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 844 Size: 2185 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2850 3561 1394 Size: 429 1027 711 3507 Fragments arranged by size: 3507 1027 711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4169 4343 65 Size: 1183 2921 174 1396 Fragments arranged by size: 2921 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2071 2939 4318 4505 1700 Size: 110 261 868 1379 187 2869 Fragments arranged by size: 2869 1379 868 261 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 2193 2468 1925 Size: 268 275 5131 Fragments arranged by size: 5131 275 268 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 2329 2690 2880 2895 794 Size: 1535 361 190 15 3573 Fragments arranged by size: 3573 1535 361 190 15 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2500 2549 2500 Size: 49 5625 BstYI r'GATC_y Cuts at: 1972 2529 2738 3004 3371 3756 3773 5225 5311 Size: 557 209 266 367 385 17 1452 86 Cuts at: 5311 5323 5669 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 557 385 367 346 266 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2385 2649 2709 2805 513 Size: 552 939 381 264 60 96 3382 Fragments arranged by size: 3382 939 552 381 264 96 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2365 487 Size: 683 56 1139 3796 Fragments arranged by size: 3796 1139 683 56 BtsI GCAGTG_nn' Cuts at: 2227 2845 3190 3984 4004 2227 Size: 618 345 794 20 3897 Fragments arranged by size: 3897 794 618 345 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DraIII CAC_nnn'GTG Cuts at: 2923 2923 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2808 3121 4010 Size: 54 1425 50 275 18 978 313 889 Cuts at: 4010 5639 8 Size: 1629 43 Fragments arranged by size: 1629 1425 978 889 313 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2808 3121 1537 Size: 275 996 313 4090 Fragments arranged by size: 4090 996 313 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3604 1353 Size: 2251 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 3737 4334 5121 5389 1239 Size: 393 890 1215 597 787 268 1524 Fragments arranged by size: 1524 1215 890 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2399 Size: 5674 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FspI TGC'GCA Cuts at: 2435 4180 2435 Size: 1745 3929 HaeII r_GCGC'y Cuts at: 1678 2103 2163 2178 2727 2940 4821 1678 Size: 425 60 15 549 213 1881 2531 Fragments arranged by size: 2531 1881 549 425 213 60 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4315 4347 4389 4421 1373 Size: 32 135 32 2743 32 42 32 2626 Fragments arranged by size: 2743 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3018 3264 669 Size: 988 281 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 246 HindIII A'AGCT_T Cuts at: 748 2438 748 Size: 1690 3984 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KasI G'GCGC_C Cuts at: 2936 2936 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2988 4788 4972 1305 Size: 1683 1800 184 2007 Fragments arranged by size: 2007 1800 1683 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3852 Fragments arranged by size: 3852 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2483 3632 3991 4150 518 Size: 648 1218 99 1149 359 159 2042 Fragments arranged by size: 2042 1218 1149 648 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2094 2329 2651 2687 2702 2711 2807 2818 Size: 488 235 322 36 15 9 96 11 Cuts at: 2818 3090 3767 4915 5160 1606 Size: 272 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 488 322 272 245 235 96 36 15 11 9 NaeI GCC'GGC Cuts at: 2073 2941 2073 Size: 868 4806 NarI GG'CG_CC Cuts at: 2937 2937 Size: 5674 NcoI C'CATG_G Cuts at: 513 1065 2004 2385 513 Size: 552 939 381 3802 Fragments arranged by size: 3802 939 552 381 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NgoMIV G'CCGG_C Cuts at: 2071 2939 2071 Size: 868 4806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 2808 3121 2808 Size: 313 5361 NspI r_CATG'y Cuts at: 1132 1229 2368 2878 2896 3032 4577 1132 Size: 97 1139 510 18 136 1545 2229 Fragments arranged by size: 2229 1545 1139 510 136 97 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PmlI CAC'GTG Cuts at: 2923 2923 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PstI C_TGCA'G Cuts at: 830 2325 2707 2891 3026 830 Size: 1495 382 184 135 3478 Fragments arranged by size: 3478 1495 382 184 135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2329 2329 Size: 5674 SacII CC_GC'GG Cuts at: 2652 2712 2808 2652 Size: 60 96 5518 Fragments arranged by size: 5518 96 60 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2321 2703 2887 3022 4157 4838 5029 Size: 219 1276 382 184 135 1135 681 191 Cuts at: 5029 826 Size: 1471 Fragments arranged by size: 1471 1276 1135 681 382 219 191 184 135 SfoI GGC'GCC Cuts at: 2938 2938 Size: 5674 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 2786 3778 4679 4941 820 Size: 197 926 393 450 992 901 262 1553 Fragments arranged by size: 1553 992 926 901 450 393 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 2368 2878 3032 1132 Size: 1236 510 154 3774 Fragments arranged by size: 3774 1236 510 154 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2004 2385 2970 3113 513 Size: 552 939 381 585 143 3074 Fragments arranged by size: 3074 939 585 552 381 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2743 3701 3718 3871 4056 4501 325 Size: 1255 1163 958 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1163 958 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3920 5632 96 Size: 274 80 33 51 1443 1943 1712 138 Fragments arranged by size: 1943 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 2461 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 3803 3803 Size: 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI