Restriction map for
pFN21AB9635
(Linear) (Six-base) MAPSORT of: KIBB9635.seq Check: 4963 from: 1 to: 969
KIBB9635.orf
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AccI GT'mk_AC
Cuts at: 0 253 969
Size: 253 716
AflIII A'CryG_T
Cuts at: 0 917 969
Size: 917 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 376 969
Size: 376 593
AlwNI CAG_nnn'CTG
Cuts at: 0 324 685 969
Size: 324 361 284
Fragments arranged by size:
361 324 284
ApaLI G'TGCA_C
Cuts at: 0 649 969
Size: 649 320
AvaI C'yCGr_G
Cuts at: 0 129 969
Size: 129 840
BamHI G'GATC_C
Cuts at: 0 733 969
Size: 733 236
BanI G'GyrC_C
Cuts at: 0 109 897 931 969
Size: 109 788 34 38
Fragments arranged by size:
788 109 38 34
BanII G_rGCy'C
Cuts at: 0 50 181 969
Size: 50 131 788
Fragments arranged by size:
788 131 50
BbeI G_GCGC'C
Cuts at: 0 935 969
Size: 935 34
BglI GCCn_nnn'nGGC
Cuts at: 0 682 969
Size: 682 287
BglII A'GATC_T
Cuts at: 0 524 969
Size: 524 445
BlpI GC'TnA_GC
Cuts at: 0 151 969
Size: 151 818
Bme1580I G_kGCm'C
Cuts at: 0 112 653 969
Size: 112 541 316
Fragments arranged by size:
541 316 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 113 870 969
Size: 113 757 99
Fragments arranged by size:
757 113 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 969
Size: 517 452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 766 969
Size: 316 450 203
Fragments arranged by size:
450 316 203
BsaAI yAC'GTr
Cuts at: 0 918 969
Size: 918 51
BsaHI Gr'CG_yC
Cuts at: 0 11 932 962 969
Size: 11 921 30 7
Fragments arranged by size:
921 30 11 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 969
Size: 514 455
BseYI C'CCAG_C
Cuts at: 0 813 969
Size: 813 156
BsiEI CG_ry'CG
Cuts at: 0 752 806 969
Size: 752 54 163
Fragments arranged by size:
752 163 54
BsiHKAI G_wGCw'C
Cuts at: 0 653 969
Size: 653 316
BsiWI C'GTAC_G
Cuts at: 0 407 969
Size: 407 562
BsmBI CGTCTCn'nnnn_
Cuts at: 0 334 969
Size: 334 635
Bsp1286I G_dGCh'C
Cuts at: 0 50 112 181 653 969
Size: 50 62 69 472 316
Fragments arranged by size:
472 316 69 62 50
BsrBI CCG'CTC
Cuts at: 0 845 969
Size: 845 124
BsrFI r'CCGG_y
Cuts at: 0 66 934 969
Size: 66 868 35
Fragments arranged by size:
868 66 35
BssHII G'CGCG_C
Cuts at: 0 188 463 969
Size: 188 275 506
Fragments arranged by size:
506 275 188
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 685 875 890 969
Size: 324 361 190 15 79
Fragments arranged by size:
361 324 190 79 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 544 969
Size: 495 49 425
Fragments arranged by size:
495 425 49
BstYI r'GATC_y
Cuts at: 0 524 733 969
Size: 524 209 236
Fragments arranged by size:
524 236 209
BtgI C'CryG_G
Cuts at: 0 380 644 704 800 969
Size: 380 264 60 96 169
Fragments arranged by size:
380 264 169 96 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 969
Size: 360 609
BtsI GCAGTG_nn'
Cuts at: 0 222 840 969
Size: 222 618 129
Fragments arranged by size:
618 222 129
DraIII CAC_nnn'GTG
Cuts at: 0 918 969
Size: 918 51
EaeI y'GGCC_r
Cuts at: 0 803 969
Size: 803 166
EagI C'GGCC_G
Cuts at: 0 803 969
Size: 803 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 969
Size: 517 452
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 969
Size: 394 575
FspI TGC'GCA
Cuts at: 0 430 969
Size: 430 539
HaeII r_GCGC'y
Cuts at: 0 98 158 173 722 935 969
Size: 98 60 15 549 213 34
Fragments arranged by size:
549 213 98 60 34 15
HindIII A'AGCT_T
Cuts at: 0 433 969
Size: 433 536
KasI G'GCGC_C
Cuts at: 0 931 969
Size: 931 38
MslI CAynn'nnrTG
Cuts at: 0 379 478 969
Size: 379 99 491
Fragments arranged by size:
491 379 99
MspA1I CmG'CkG
Cuts at: 0 89 324 646 682 697 706 802 813
Size: 89 235 322 36 15 9 96 11
Cuts at: 813 969
Size: 156
Fragments arranged by size:
322 235 156 96 89 36 15 11
9
NaeI GCC'GGC
Cuts at: 0 68 936 969
Size: 68 868 33
Fragments arranged by size:
868 68 33
NarI GG'CG_CC
Cuts at: 0 932 969
Size: 932 37
NcoI C'CATG_G
Cuts at: 0 380 969
Size: 380 589
NgoMIV G'CCGG_C
Cuts at: 0 66 934 969
Size: 66 868 35
Fragments arranged by size:
868 66 35
NotI GC'GGCC_GC
Cuts at: 0 803 969
Size: 803 166
NspI r_CATG'y
Cuts at: 0 363 873 891 969
Size: 363 510 18 78
Fragments arranged by size:
510 363 78 18
PmlI CAC'GTG
Cuts at: 0 918 969
Size: 918 51
PstI C_TGCA'G
Cuts at: 0 320 702 886 969
Size: 320 382 184 83
Fragments arranged by size:
382 320 184 83
PvuII CAG'CTG
Cuts at: 0 324 969
Size: 324 645
SacII CC_GC'GG
Cuts at: 0 647 707 803 969
Size: 647 60 96 166
Fragments arranged by size:
647 166 96 60
SfcI C'TryA_G
Cuts at: 0 316 698 882 969
Size: 316 382 184 87
Fragments arranged by size:
382 316 184 87
SfoI GGC'GCC
Cuts at: 0 933 969
Size: 933 36
SmlI C'TyrA_G
Cuts at: 0 331 781 969
Size: 331 450 188
Fragments arranged by size:
450 331 188
SphI G_CATG'C
Cuts at: 0 363 873 969
Size: 363 510 96
Fragments arranged by size:
510 363 96
StyI C'CwwG_G
Cuts at: 0 380 969
Size: 380 589
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 738 969
Size: 738 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 969
Size: 456 513
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaLI AvaI BamHI BanI
BanII BbeI BglI BglII BlpI Bme1580I BmrI BpmI
BpuEI BsaAI BsaHI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BsrBI BsrFI BssHII BstAPI BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EagI Eco57MI EcoNI
FspI HaeII HindIII KasI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PmlI PstI PvuII SacII
SfcI SfoI SmlI SphI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI
BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaWI BsaXI
BsgI BsmI BspEI BspHI BspMI BsrDI BsrGI BssSI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI
EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HpaI KpnI MfeI MluI MmeI
MscI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI