################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AhdI Alw44I AlwNI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbeI BbrPI BcgI BclI BfaI BfrI BlnI BsaAI BsaHI BsaI BseAI BsgI BsiWI BsmAI BsmBI BspEI BspMI BssHII BssSI BstBI BstEII BstPI BstXI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT14I EcoT22I FbaI FseI Hin1I HindIII HpaI KasI KpnI KspI MaeI MluI MluNI MroI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI Psp1406I PstI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StyI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 498 ACATGT A^C[GA][CT]GT AgeI 1636 ACCGGT A^CCGGT AocI 970 CCTCAGG CC^T[ACGT]AGG AspHI 922 GAGCTC G[AT]GC[AT]^C AvaII 2088 GGACC G^G[AT]CC AviII 29 TGCGCA TGC^GCA BglI 128 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 423 AGATCT A^GATCT BpmI 905 CTGGAG CTGGAG(16/14) BsaBI 508 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1636 ACCGGT [AT]^CCGG[AT] BsiEI 1548 CGATCG CG[GA][CT]^CG BsiHKAI 922 GAGCTC G[AT]GC[AT]^C BspDI 2414 ATCGAT AT^CGAT BspLU11I 498 ACATGT A^CATGT BsrDI 1584 CATTGC GCAATG(2/0) BsrFI 1636 ACCGGT [GA]^CCGG[CT] Bst1107I 2191 GTATAC GTA^TAC BstAPI 1332 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 423 AGATCT [GA]^GATC[CT] BstZ17I 2191 GTATAC GTA^TAC Bsu36I 970 CCTCAGG CC^T[ACGT]AGG Cfr10I 1636 ACCGGT [GA]^CCGG[CT] ClaI 2414 ATCGAT AT^CGAT DraIII 2713 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 970 CCTCAGG CC^T[ACGT]AGG EcoNI 742 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2439 GAATTC G^AATTC EcoRV 2150 GATATC GAT^ATC FspI 29 TGCGCA TGC^GCA HaeII 692 AGCGCC [GA]GCGC^[CT] HgaI 1544 GACGC GACGC(5/10) HincII 986 GTCAAC GT[CT]^[GA]AC HindII 986 GTCAAC GT[CT]^[GA]AC MamI 508 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 423 AGATCT [GA]^GATC[CT] MslI 730 CATGATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NruI 285 TCGCGA TCG^CGA PinAI 1636 ACCGGT A^CCGGT PpuMI 2087 GGGACCC [GA]G^G[AT]CC[CT] PvuI 1548 CGATCG CGAT^CG SacI 922 GAGCTC GAGCT^C SfcI 1691 CTACAG C^T[GA][CT]AG SphI 1569 GCATGC GCATG^C XhoII 423 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 1616 GTAGAC GT^[AC][GT]AC 2191 GTATAC AlwI 1081 GGATC GGATC(4/5) 2685 GGATC BbsI 1296 GTCTTC GAAGAC(2/6) 2322 GTCTTC BbvCI 217 GCTGAGG CCTCAGC(-5/-2) 859 GCTGAGG BciVI 507 GGATAC GTATCC(6/5) 2580 GGATAC BlpI 318 GCTCAGC GC^T[ACGT]AGC 924 GCTCAGC Bpu1102I 318 GCTCAGC GC^T[ACGT]AGC 924 GCTCAGC BpuAI 1296 GTCTTC GAAGAC(2/6) 2322 GTCTTC BsmFI 2087 GGGAC GGGAC(10/14) 1120 GTCCC BsmI 2166 GAATGC GAATGC(1/-1) 1055 GCATTC Bsp1407I 1424 TGTACA T^GTACA 2568 TGTACA BspHI 729 TCATGA T^CATGA 2510 TCATGA BsrBI 58 GAGCGG CCGCTC(-3/-3) 1591 GAGCGG BsrGI 1424 TGTACA T^GTACA 2568 TGTACA CelII 318 GCTCAGC GC^T[ACGT]AGC 924 GCTCAGC MaeII 26 ACGT A^CGT 1578 ACGT MfeI 1781 CAATTG C^AATTG 2487 CAATTG MspA1I 464 CAGCTG C[AC]G^C[GT]G 1420 CAGCTG MunI 1781 CAATTG C^AATTG 2487 CAATTG PflMI 592 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2498 CCAAACAATGG PvuII 464 CAGCTG CAG^CTG 1420 CAGCTG RcaI 729 TCATGA T^CATGA 2510 TCATGA SapI 55 GAAGAGC GCTCTTC(1/4) 919 GAAGAGC SspBI 1424 TGTACA T^GTACA 2568 TGTACA StuI 280 AGGCCT AGG^CCT 1662 AGGCCT Van91I 592 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2498 CCAAACAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApaI ApoI AseI AsnI AvaI BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI