(Circular) (Six-base) MAPSORT of: pFN21AE3323.seq Check: 9714 from: 1 to: 7456 pFN21AE3323 7456 bp With 182 enzymes: SgfI * March 28, 2012 12:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5266 278 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3622 4197 4799 1937 Size: 1685 575 602 4594 Fragments arranged by size: 4594 1685 602 575 Acc65I G'GTAC_C Cuts at: 4777 4777 Size: 7456 AclI AA'CG_TT Cuts at: 1563 5583 5956 1563 Size: 4020 373 3063 Fragments arranged by size: 4020 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2291 3884 4146 4314 4340 5519 6903 7171 Size: 659 1593 262 168 26 1179 1384 268 Cuts at: 7171 1632 Size: 1917 Fragments arranged by size: 1917 1593 1384 1179 659 268 262 168 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7259 AflIII A'CryG_T Cuts at: 1203 2503 6355 7332 1203 Size: 1300 3852 977 1327 Fragments arranged by size: 3852 1327 1300 977 AgeI A'CCGG_T Cuts at: 3641 6287 3641 Size: 2646 4810 AhdI GACnn_n'nnGTC Cuts at: 6185 6185 Size: 7456 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7456 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7456 AlwNI CAG_nnn'CTG Cuts at: 5199 6771 5199 Size: 1572 5884 ApaI G_GGCC'C Cuts at: 2135 2155 2180 2135 Size: 20 25 7411 Fragments arranged by size: 7411 25 20 ApaLI G'TGCA_C Cuts at: 5513 6669 5513 Size: 1156 6300 ApoI r'AATT_y Cuts at: 1493 2658 3329 3732 4288 4444 4730 4765 4991 Size: 1165 671 403 556 156 286 35 226 Cuts at: 4991 5143 7252 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1165 671 556 403 286 226 156 152 35 AseI AT'TA_AT Cuts at: 160 1032 2967 3399 4145 6010 160 Size: 872 1935 432 746 1865 1606 Fragments arranged by size: 1935 1865 1606 872 746 432 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 AvaI C'yCGr_G Cuts at: 1870 1943 2179 2209 2216 2310 2709 4781 1870 Size: 73 236 30 7 94 399 2072 4545 Fragments arranged by size: 4545 2072 399 236 94 73 30 7 BamHI G'GATC_C Cuts at: 4786 5153 4786 Size: 367 7089 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 3388 3526 4777 6232 Size: 325 217 627 518 1083 138 1251 1455 Cuts at: 6232 618 Size: 1842 Fragments arranged by size: 1842 1455 1251 1083 627 518 325 217 138 BanII G_rGCy'C Cuts at: 1391 2135 2155 2180 2259 2931 4775 1391 Size: 744 20 25 79 672 1844 4072 Fragments arranged by size: 4072 1844 744 672 79 25 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3294 4320 928 Size: 971 1395 1026 4064 Fragments arranged by size: 4064 1395 1026 971 BbvCI CC'TCA_GC Cuts at: 2223 2865 2223 Size: 642 6814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5647 5681 1903 Size: 34 3710 34 3678 Fragments arranged by size: 3710 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2506 4579 5348 6558 2506 Size: 2073 769 1210 3404 Fragments arranged by size: 3404 2073 1210 769 BclI T'GATC_A Cuts at: 1548 1548 Size: 7456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2139 6067 136 Size: 107 122 71 631 605 467 3928 1525 Fragments arranged by size: 3928 1525 631 605 467 122 107 71 BglII A'GATC_T Cuts at: 2428 7451 2428 Size: 5023 2433 BlpI GC'TnA_GC Cuts at: 2324 2930 4873 2324 Size: 606 1943 4907 Fragments arranged by size: 4907 1943 606 Bme1580I G_kGCm'C Cuts at: 1790 2117 2135 2155 2180 2310 3562 5517 6673 Size: 327 18 20 25 130 1252 1955 1156 Cuts at: 6673 1790 Size: 2573 Fragments arranged by size: 2573 1955 1252 1156 327 130 25 20 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 5187 6145 Size: 416 53 83 399 24 1718 2052 958 Cuts at: 6145 442 Size: 1753 Fragments arranged by size: 2052 1753 1718 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2931 6116 1239 Size: 1692 3185 2579 Fragments arranged by size: 3185 2579 1692 Bpu10I CC'TnA_GC Cuts at: 2223 2865 2223 Size: 642 6814 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 3389 3944 5581 6446 6744 2393 Size: 996 555 1637 865 298 3105 Fragments arranged by size: 3105 1637 996 865 555 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6119 882 Size: 617 4620 2219 Fragments arranged by size: 4620 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5247 493 Size: 711 4043 2702 Fragments arranged by size: 4043 2702 711 BsaBI GATnn'nnATC Cuts at: 2517 7450 2517 Size: 4933 2523 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5263 5645 6280 275 Size: 53 83 186 936 3730 382 635 1451 Fragments arranged by size: 3730 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3641 5889 6287 6561 6708 7119 3641 Size: 2248 398 274 147 411 3978 Fragments arranged by size: 3978 2248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3601 3631 3888 3901 3918 Size: 30 165 30 2198 30 257 13 17 Cuts at: 3918 3931 1178 Size: 13 4703 Fragments arranged by size: 4703 2198 257 165 30 30 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2354 2721 3896 3909 1186 Size: 907 261 367 1175 13 4733 Fragments arranged by size: 4733 1175 907 367 261 13 BseYI C'CCAG_C Cuts at: 2173 6659 7155 2173 Size: 4486 496 2474 Fragments arranged by size: 4486 2474 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6883 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4906 5667 6695 1540 Size: 275 186 1555 1350 761 1028 2301 Fragments arranged by size: 2301 1555 1350 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2931 5517 5602 6673 2931 Size: 2586 85 1071 3714 Fragments arranged by size: 3714 2586 1071 85 BsmI GAATG_Cn' Cuts at: 3060 4177 4967 5060 3060 Size: 1117 790 93 5456 Fragments arranged by size: 5456 1117 790 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2135 2155 2180 2259 2310 2931 Size: 399 327 18 20 25 79 51 621 Cuts at: 2931 3562 4775 5517 5602 6673 1391 Size: 631 1213 742 85 1071 2174 Fragments arranged by size: 2174 1213 1071 742 631 621 399 327 85 79 51 25 20 18 BspEI T'CCGG_A Cuts at: 7119 7119 Size: 7456 BspHI T'CATG_A Cuts at: 2734 4515 5345 7075 2734 Size: 1781 830 1730 3115 Fragments arranged by size: 3115 1781 1730 830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4811 844 Size: 3967 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2065 3598 5343 1394 Size: 429 242 1533 1745 3507 Fragments arranged by size: 3507 1745 1533 429 242 BsrDI GCAATG_nn' Cuts at: 65 1248 3588 5951 6125 65 Size: 1183 2340 2363 174 1396 Fragments arranged by size: 2363 2340 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3641 6100 6287 1700 Size: 110 1831 2459 187 2869 Fragments arranged by size: 2869 2459 1831 187 110 BsrGI T'GTAC_A Cuts at: 96 3429 4573 96 Size: 3333 1144 2979 Fragments arranged by size: 3333 2979 1144 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7456 BssSI C'ACGA_G Cuts at: 5516 6528 5516 Size: 1012 6444 BstAPI GCAn_nnn'nTGC Cuts at: 794 3343 794 Size: 2549 4907 BstBI TT'CG_AA Cuts at: 6260 6260 Size: 7456 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7456 BstYI r'GATC_y Cuts at: 1972 2428 4786 5153 5538 5555 7007 7093 7105 Size: 456 2358 367 385 17 1452 86 12 Cuts at: 7105 7451 1972 Size: 346 1977 Fragments arranged by size: 2358 1977 1452 456 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4198 4198 Size: 7456 Bsu36I CC'TnA_GG Cuts at: 2976 2976 Size: 7456 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6904 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2088 487 Size: 683 56 862 5855 Fragments arranged by size: 5855 862 683 56 BtsI GCAGTG_nn' Cuts at: 4972 5766 5786 4972 Size: 794 20 6642 Fragments arranged by size: 6642 794 20 ClaI AT'CG_AT Cuts at: 1554 4420 1554 Size: 2866 4590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4107 4142 529 Size: 35 3543 35 3843 Fragments arranged by size: 3843 3543 35 35 DraI TTT'AAA Cuts at: 4760 4760 Size: 7456 DraIII CAC_nnn'GTG Cuts at: 4723 4723 Size: 7456 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6463 809 Size: 507 5147 1802 Fragments arranged by size: 5147 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4903 5792 7421 Size: 54 1425 50 275 18 3073 889 1629 Cuts at: 7421 8 Size: 43 Fragments arranged by size: 3073 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4903 1537 Size: 275 3091 4090 Fragments arranged by size: 4090 3091 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2055 2118 2919 5386 1353 Size: 696 6 63 801 2467 3423 Fragments arranged by size: 3423 2467 801 696 63 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6043 6417 6563 387 Size: 1064 138 4454 374 146 1280 Fragments arranged by size: 4454 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2929 2929 Size: 7456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2931 3884 4146 4314 4340 5519 Size: 393 659 640 953 262 168 26 1179 Cuts at: 5519 6116 6903 7171 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1179 953 787 659 640 597 393 268 262 168 26 EcoNI CCTnn'n_nnAGG Cuts at: 2751 2751 Size: 7456 EcoO109I rG'GnC_Cy Cuts at: 2132 2151 2152 4093 2132 Size: 19 1 1941 5495 Fragments arranged by size: 5495 1941 19 1 EcoRI G'AATT_C Cuts at: 4444 4765 5143 4444 Size: 321 378 6757 Fragments arranged by size: 6757 378 321 EcoRV GAT'ATC Cuts at: 4157 4157 Size: 7456 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2447 2777 3383 3785 2447 Size: 330 606 402 6118 Fragments arranged by size: 6118 606 402 330 FspI TGC'GCA Cuts at: 2036 5962 2036 Size: 3926 3530 HaeII r_GCGC'y Cuts at: 1678 2701 6603 1678 Size: 1023 3902 2531 Fragments arranged by size: 3902 2531 1023 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2523 2555 3086 3118 3339 Size: 32 135 32 951 32 531 32 221 Cuts at: 3339 3371 3601 3633 3640 3672 3888 3920 4413 Size: 32 230 32 7 32 216 32 493 Cuts at: 4413 4445 4528 4560 6097 6129 6171 6203 1373 Size: 32 83 32 1537 32 42 32 2626 Fragments arranged by size: 2626 1537 951 531 493 230 221 216 135 83 42 32 32 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2993 4800 5046 669 Size: 988 281 1055 1807 246 3079 Fragments arranged by size: 3079 1807 1055 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7456 HpaI GTT'AAC Cuts at: 5046 5046 Size: 7456 KpnI G_GTAC'C Cuts at: 4781 4781 Size: 7456 MfeI C'AATT_G Cuts at: 3786 4492 5055 3786 Size: 706 563 6187 Fragments arranged by size: 6187 706 563 MluI A'CGCG_T Cuts at: 7332 7332 Size: 7456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3180 6570 6754 1305 Size: 1875 3390 184 2007 Fragments arranged by size: 3390 2007 1875 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5634 Fragments arranged by size: 5634 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2739 5414 5773 5932 518 Size: 648 1573 2675 359 159 2042 Fragments arranged by size: 2675 2042 1573 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2471 3427 4872 5549 6697 6942 1606 Size: 865 956 1445 677 1148 245 2120 Fragments arranged by size: 2120 1445 1148 956 865 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6904 NdeI CA'TA_TG Cuts at: 387 387 Size: 7456 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7456 NotI GC'GGCC_GC Cuts at: 4903 4903 Size: 7456 NruI TCG'CGA Cuts at: 2292 2292 Size: 7456 NspI r_CATG'y Cuts at: 1132 1229 2507 2684 3578 4814 6359 1132 Size: 97 1278 177 894 1236 1545 2229 Fragments arranged by size: 2229 1545 1278 1236 894 177 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7456 PciI A'CATG_T Cuts at: 2503 6355 2503 Size: 3852 3604 PflMI CCAn_nnn'nTGG Cuts at: 1108 2603 4509 1108 Size: 1495 1906 4055 Fragments arranged by size: 4055 1906 1495 PfoI T'CCnGG_A Cuts at: 2331 2650 2331 Size: 319 7137 PmeI GTTT'AAAC Cuts at: 4760 4760 Size: 7456 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5525 5557 7038 7070 5525 Size: 32 1481 32 5911 Fragments arranged by size: 5911 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4093 4093 Size: 7456 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7456 PsiI TTA'TAA Cuts at: 5026 5026 Size: 7456 PspOMI G'GGCC_C Cuts at: 2131 2151 2176 2131 Size: 20 25 7411 Fragments arranged by size: 7411 25 20 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4597 4629 4597 Size: 32 7424 PstI C_TGCA'G Cuts at: 830 4808 830 Size: 3978 3478 PvuI CG_AT'CG Cuts at: 2001 3556 2001 Size: 1555 5901 PvuII CAG'CTG Cuts at: 2471 3427 2471 Size: 956 6500 SacI G_AGCT'C Cuts at: 2931 2931 Size: 7456 SalI G'TCGA_C Cuts at: 1936 4798 1936 Size: 2862 4594 SanDI GG'GwC_CC Cuts at: 4093 4093 Size: 7456 SapI GCTCTTCn'nnn_ Cuts at: 2055 2919 2055 Size: 864 6592 SbfI CC_TGCA'GG Cuts at: 4808 4808 Size: 7456 ScaI AGT'ACT Cuts at: 5704 7416 5704 Size: 1712 5744 SfcI C'TryA_G Cuts at: 826 1045 3696 4804 5939 6620 6811 826 Size: 219 2651 1108 1135 681 191 1471 Fragments arranged by size: 2651 1471 1135 1108 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7456 SmaI CCC'GGG Cuts at: 1872 2181 2211 2312 4783 1872 Size: 309 30 101 2471 4545 Fragments arranged by size: 4545 2471 309 101 30 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 3368 3923 5560 6461 6723 Size: 197 926 465 960 555 1637 901 262 Cuts at: 6723 820 Size: 1553 Fragments arranged by size: 1637 1553 960 926 901 555 465 262 197 SnaBI TAC'GTA Cuts at: 493 5247 493 Size: 4754 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7456 SphI G_CATG'C Cuts at: 1132 3578 4814 1132 Size: 2446 1236 3774 Fragments arranged by size: 3774 2446 1236 SspI AAT'ATT Cuts at: 5 52 5380 5 Size: 47 5328 2081 Fragments arranged by size: 5328 2081 47 StuI AGG'CCT Cuts at: 1517 2287 3669 1517 Size: 770 1382 5304 Fragments arranged by size: 5304 1382 770 StyI C'CwwG_G Cuts at: 513 1065 4895 513 Size: 552 3830 3074 Fragments arranged by size: 3830 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5483 5500 5653 5838 6283 325 Size: 1255 3903 17 153 185 445 1498 Fragments arranged by size: 3903 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3012 3429 4573 Size: 274 80 33 51 1443 1035 417 1144 Cuts at: 4573 5702 7414 96 Size: 1129 1712 138 Fragments arranged by size: 1712 1443 1144 1129 1035 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3901 3933 7416 7448 3901 Size: 32 3483 32 3909 Fragments arranged by size: 3909 3483 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7456 XbaI T'CTAG_A Cuts at: 4792 4792 Size: 7456 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7456 XmaI C'CCGG_G Cuts at: 1870 2179 2209 2310 4781 1870 Size: 309 30 101 2471 4545 Fragments arranged by size: 4545 2471 309 101 30 XmnI GAAnn'nnTTC Cuts at: 5585 5585 Size: 7456 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5264 276 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI