(Linear) (Six-base) MAPSORT of: KIEE3323.seq Check: 1730 from: 1 to: 2751 KIEE3323.orf With 182 enzymes: SgfI * March 22, 2012 16:29 .. AccI GT'mk_AC Cuts at: 0 1617 2192 2751 Size: 1617 575 559 Fragments arranged by size: 1617 575 559 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 1879 2141 2309 2335 2751 Size: 286 1593 262 168 26 416 Fragments arranged by size: 1593 416 286 262 168 26 AflIII A'CryG_T Cuts at: 0 498 2751 Size: 498 2253 AgeI A'CCGG_T Cuts at: 0 1636 2751 Size: 1636 1115 ApaI G_GGCC'C Cuts at: 0 130 150 175 2751 Size: 130 20 25 2576 Fragments arranged by size: 2576 130 25 20 ApoI r'AATT_y Cuts at: 0 653 1324 1727 2283 2439 2725 2751 Size: 653 671 403 556 156 286 26 Fragments arranged by size: 671 653 556 403 286 156 26 AseI AT'TA_AT Cuts at: 0 962 1394 2140 2751 Size: 962 432 746 611 Fragments arranged by size: 962 746 611 432 AvaI C'yCGr_G Cuts at: 0 174 204 211 305 704 2751 Size: 174 30 7 94 399 2047 Fragments arranged by size: 2047 399 174 94 30 7 BanI G'GyrC_C Cuts at: 0 300 1383 1521 2751 Size: 300 1083 138 1230 Fragments arranged by size: 1230 1083 300 138 BanII G_rGCy'C Cuts at: 0 130 150 175 254 926 2751 Size: 130 20 25 79 672 1825 Fragments arranged by size: 1825 672 130 79 25 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 1289 2315 2751 Size: 1289 1026 436 Fragments arranged by size: 1289 1026 436 BbvCI CC'TCA_GC Cuts at: 0 218 860 2751 Size: 218 642 1891 Fragments arranged by size: 1891 642 218 BciVI GTATCCnnnnn_n' Cuts at: 0 501 2574 2751 Size: 501 2073 177 Fragments arranged by size: 2073 501 177 BglI GCCn_nnn'nGGC Cuts at: 0 134 2751 Size: 134 2617 BglII A'GATC_T Cuts at: 0 423 2751 Size: 423 2328 BlpI GC'TnA_GC Cuts at: 0 319 925 2751 Size: 319 606 1826 Fragments arranged by size: 1826 606 319 Bme1580I G_kGCm'C Cuts at: 0 112 130 150 175 305 1557 2751 Size: 112 18 20 25 130 1252 1194 Fragments arranged by size: 1252 1194 130 112 25 20 18 BmrI ACTGGGnnnn_n' Cuts at: 0 1130 2751 Size: 1130 1621 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 926 2751 Size: 926 1825 Bpu10I CC'TnA_GC Cuts at: 0 218 860 2751 Size: 218 642 1891 Fragments arranged by size: 1891 642 218 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 1384 1939 2751 Size: 388 996 555 812 Fragments arranged by size: 996 812 555 388 BsaBI GATnn'nnATC Cuts at: 0 512 2751 Size: 512 2239 BsaWI w'CCGG_w Cuts at: 0 1636 2751 Size: 1636 1115 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1596 1626 1883 1896 1913 1926 2751 Size: 1596 30 257 13 17 13 825 Fragments arranged by size: 1596 825 257 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 349 716 1891 1904 2751 Size: 88 261 367 1175 13 847 Fragments arranged by size: 1175 847 367 261 88 13 BseYI C'CCAG_C Cuts at: 0 168 2751 Size: 168 2583 BsiEI CG_ry'CG Cuts at: 0 1551 2751 Size: 1551 1200 BsiHKAI G_wGCw'C Cuts at: 0 926 2751 Size: 926 1825 BsmI GAATG_Cn' Cuts at: 0 1055 2172 2751 Size: 1055 1117 579 Fragments arranged by size: 1117 1055 579 Bsp1286I G_dGCh'C Cuts at: 0 112 130 150 175 254 305 926 1557 Size: 112 18 20 25 79 51 621 631 Cuts at: 1557 2751 Size: 1194 Fragments arranged by size: 1194 631 621 112 79 51 25 20 18 BspHI T'CATG_A Cuts at: 0 729 2510 2751 Size: 729 1781 241 Fragments arranged by size: 1781 729 241 BsrBI CCG'CTC Cuts at: 0 60 1593 2751 Size: 60 1533 1158 Fragments arranged by size: 1533 1158 60 BsrDI GCAATG_nn' Cuts at: 0 1583 2751 Size: 1583 1168 BsrFI r'CCGG_y Cuts at: 0 1636 2751 Size: 1636 1115 BsrGI T'GTAC_A Cuts at: 0 1424 2568 2751 Size: 1424 1144 183 Fragments arranged by size: 1424 1144 183 BstAPI GCAn_nnn'nTGC Cuts at: 0 1338 2751 Size: 1338 1413 BstYI r'GATC_y Cuts at: 0 423 2751 Size: 423 2328 BstZ17I GTA'TAC Cuts at: 0 2193 2751 Size: 2193 558 Bsu36I CC'TnA_GG Cuts at: 0 971 2751 Size: 971 1780 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 83 2751 Size: 83 2668 ClaI AT'CG_AT Cuts at: 0 2415 2751 Size: 2415 336 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2102 2137 2751 Size: 2102 35 614 Fragments arranged by size: 2102 614 35 DraIII CAC_nnn'GTG Cuts at: 0 2718 2751 Size: 2718 33 EarI CTCTTCn'nnn_ Cuts at: 0 44 50 113 914 2751 Size: 44 6 63 801 1837 Fragments arranged by size: 1837 801 63 44 6 EcoICRI GAG'CTC Cuts at: 0 924 2751 Size: 924 1827 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 926 1879 2141 2309 2335 2751 Size: 286 640 953 262 168 26 416 Fragments arranged by size: 953 640 416 286 262 168 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 746 2751 Size: 746 2005 EcoO109I rG'GnC_Cy Cuts at: 0 127 146 147 2088 2751 Size: 127 19 1 1941 663 Fragments arranged by size: 1941 663 127 19 1 EcoRI G'AATT_C Cuts at: 0 2439 2751 Size: 2439 312 EcoRV GAT'ATC Cuts at: 0 2152 2751 Size: 2152 599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 442 772 1378 1780 2751 Size: 442 330 606 402 971 Fragments arranged by size: 971 606 442 402 330 FspI TGC'GCA Cuts at: 0 31 2751 Size: 31 2720 HaeII r_GCGC'y Cuts at: 0 696 2751 Size: 696 2055 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 518 550 1081 1113 1334 1366 1596 1628 Size: 518 32 531 32 221 32 230 32 Cuts at: 1628 1635 1667 1883 1915 2408 2440 2523 2555 Size: 7 32 216 32 493 32 83 32 Cuts at: 2555 2751 Size: 196 Fragments arranged by size: 531 518 493 230 221 216 196 83 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 0 988 2751 Size: 988 1763 MfeI C'AATT_G Cuts at: 0 1781 2487 2751 Size: 1781 706 264 Fragments arranged by size: 1781 706 264 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1175 2751 Size: 1175 1576 MslI CAynn'nnrTG Cuts at: 0 734 2751 Size: 734 2017 MspA1I CmG'CkG Cuts at: 0 466 1422 2751 Size: 466 956 1329 Fragments arranged by size: 1329 956 466 NruI TCG'CGA Cuts at: 0 287 2751 Size: 287 2464 NspI r_CATG'y Cuts at: 0 502 679 1573 2751 Size: 502 177 894 1178 Fragments arranged by size: 1178 894 502 177 PciI A'CATG_T Cuts at: 0 498 2751 Size: 498 2253 PflMI CCAn_nnn'nTGG Cuts at: 0 598 2504 2751 Size: 598 1906 247 Fragments arranged by size: 1906 598 247 PfoI T'CCnGG_A Cuts at: 0 326 645 2751 Size: 326 319 2106 Fragments arranged by size: 2106 326 319 PpuMI rG'GwC_Cy Cuts at: 0 2088 2751 Size: 2088 663 PspOMI G'GGCC_C Cuts at: 0 126 146 171 2751 Size: 126 20 25 2580 Fragments arranged by size: 2580 126 25 20 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2592 2624 2751 Size: 2592 32 127 Fragments arranged by size: 2592 127 32 PvuI CG_AT'CG Cuts at: 0 1551 2751 Size: 1551 1200 PvuII CAG'CTG Cuts at: 0 466 1422 2751 Size: 466 956 1329 Fragments arranged by size: 1329 956 466 SacI G_AGCT'C Cuts at: 0 926 2751 Size: 926 1825 SanDI GG'GwC_CC Cuts at: 0 2088 2751 Size: 2088 663 SapI GCTCTTCn'nnn_ Cuts at: 0 50 914 2751 Size: 50 864 1837 Fragments arranged by size: 1837 864 50 SfcI C'TryA_G Cuts at: 0 1691 2751 Size: 1691 1060 SmaI CCC'GGG Cuts at: 0 176 206 307 2751 Size: 176 30 101 2444 Fragments arranged by size: 2444 176 101 30 SmlI C'TyrA_G Cuts at: 0 403 1363 1918 2751 Size: 403 960 555 833 Fragments arranged by size: 960 833 555 403 SphI G_CATG'C Cuts at: 0 1573 2751 Size: 1573 1178 StuI AGG'CCT Cuts at: 0 282 1664 2751 Size: 282 1382 1087 Fragments arranged by size: 1382 1087 282 TatI w'GTAC_w Cuts at: 0 1007 1424 2568 2751 Size: 1007 417 1144 183 Fragments arranged by size: 1144 1007 417 183 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1896 1928 2751 Size: 1896 32 823 Fragments arranged by size: 1896 823 32 XmaI C'CCGG_G Cuts at: 0 174 204 305 2751 Size: 174 30 101 2446 Fragments arranged by size: 2446 174 101 30 Enzymes that do cut: AccI AcuI AflIII AgeI ApaI ApoI AseI AvaI BanI BanII BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BstAPI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII MfeI MmeI MslI MspA1I NruI NspI PciI PflMI PfoI PpuMI PspOMI PsrI PvuI PvuII SacI SanDI SapI SfcI SmaI SmlI SphI StuI TatI TstI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BmgBI BmtI BplI BsaI BsaAI BsaHI BsgI BsiWI BsmBI BspEI BspMI BssHII BssSI BstBI BstEII BstXI BtgI BtsI DraI DrdI EaeI EagI EciI FseI FspAI HindIII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PstI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI