Restriction map for
pFN21AE9606
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiWI BsmBI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MamI MboI MfeI
MflI MroI MunI NarI NciI
NcoI NdeI NdeII NheI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AfaI 453 GTAC GT^AC
AvaI 114 CTCGAG C^[CT]CG[GA]G
AvaII 536 GGTCC G^G[AT]CC
BalI 296 TGGCCA TGG^CCA
BanII 156 GAGCTC G[GA]GC[CT]^C
BlpI 285 GCTGAGC GC^T[ACGT]AGC
BpmI 205 CTGGAG CTGGAG(16/14)
Bpu1102I 285 GCTGAGC GC^T[ACGT]AGC
BsaAI 448 TACGTG [CT]AC^GT[GA]
BsiEI 268 CGGCCG CG[GA][CT]^CG
BsiYI 257 CCGGCTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 257 CCGGCTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 114 CTCGAG C^[CT]CG[GA]G
Bsp1407I 452 TGTACA T^GTACA
BsrFI 263 GCCGGC [GA]^CCGG[CT]
BsrGI 452 TGTACA T^GTACA
BssKI 303 CCAGG ^CC[ACGT]GG
BstAPI 29 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 303 CCAGG CC^[AT]GG
CelII 285 GCTGAGC GC^T[ACGT]AGC
Cfr10I 263 GCCGGC [GA]^CCGG[CT]
Cfr13I 536 GGTCC G^G[ACGT]CC
DraII 535 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 268 CGGCCG C^GGCCG
EclXI 268 CGGCCG C^GGCCG
Eco52I 268 CGGCCG C^GGCCG
EcoO109I 535 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 303 CCAGG ^CC[AT]GG
FauI 424 GCGGG CCCGC(4/6)
MaeIII 15 GTCAC ^GT[ACGT]AC
MluI 173 ACGCGT A^CGCGT
MluNI 296 TGGCCA TGG^CCA
MscI 296 TGGCCA TGG^CCA
MseI 578 TTAA T^TAA
MslI 17 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 303 CCAGG CC^[AT]GG
NaeI 263 GCCGGC GCC^GGC
NgoMIV 263 GCCGGC G^CCGGC
NlaIV 535 GGGTCC GG[ACGT]^[ACGT]CC
NotI 267 GCGGCCGC GC^GGCCGC
NspI 149 GCATGT [GA]CATG^[CT]
PaeR7I 114 CTCGAG C^TCGAG
PpuMI 535 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 453 GTAC GT^AC
SacI 156 GAGCTC GAGCT^C
Sau96I 536 GGTCC G^G[ACGT]CC
ScrFI 303 CCAGG CC^[ACGT]GG
SspBI 452 TGTACA T^GTACA
TaqI 115 TCGA T^CGA
Tru9I 578 TTAA T^TAA
Tsp45I 15 GTCAC ^GT[GC]AC
TthHB8I 115 TCGA T^CGA
XhoI 114 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccII 174 CGCG CG^CG
423 CGCG
AflIII 173 ACGCGT A^C[GA][CT]GT
449 ACGTGT
BbsI 109 GAAGAC GAAGAC(2/6)
169 GAAGAC
BpuAI 109 GAAGAC GAAGAC(2/6)
169 GAAGAC
BstF5I 321 CATCC GGATG(2/0)
34 GGATG
BstUI 174 CGCG CG^CG
423 CGCG
FokI 321 CATCC GGATG(9/13)
34 GGATG
HapII 257 CCGG C^CGG
264 CCGG
HpaII 257 CCGG C^CGG
264 CCGG
MaeII 381 ACGT A^CGT
449 ACGT
MspI 257 CCGG C^CGG
264 CCGG
MvnI 174 CGCG CG^CG
423 CGCG
PvuII 127 CAGCTG CAG^CTG
202 CAGCTG
SmlI 114 CTCGAG C^T[CT][GA]AG
406 CTTGAG
TfiI 471 GAATC G^A[AT]TC
667 GAATC
Tsp509I 552 AATT ^AATT
674 AATT
TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^
504 CAGTG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BseRI BsiHKAI BsmAI Bsp1286I Cac8I
CfoI DdeI EaeI Fnu4HI HaeIII
HgaI HhaI HinP1I HinfI ItaI
MboII MnlI MspA1I MwoI NlaIII
PleI SfaNI TseI