(Circular) (Six-base) MAPSORT of: pFN21AE9606.seq Check: 4336 from: 1 to: 5404 pFN21AE9606 5404 bp With 182 enzymes: SgfI * March 28, 2012 12:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3214 278 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2747 1937 Size: 810 4594 Acc65I G'GTAC_C Cuts at: 2725 2725 Size: 5404 AclI AA'CG_TT Cuts at: 1563 3531 3904 1563 Size: 1968 373 3063 Fragments arranged by size: 3063 1968 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3467 4851 5119 1632 Size: 1835 1384 268 1917 Fragments arranged by size: 1917 1835 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5207 AflIII A'CryG_T Cuts at: 1203 2178 2454 4303 5280 1203 Size: 975 276 1849 977 1327 Fragments arranged by size: 1849 1327 977 975 276 AgeI A'CCGG_T Cuts at: 4235 4235 Size: 5404 AhdI GACnn_n'nnGTC Cuts at: 4133 4133 Size: 5404 AleI CACnn'nnGTG Cuts at: 1166 2026 1166 Size: 860 4544 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5404 AlwNI CAG_nnn'CTG Cuts at: 3147 4719 3147 Size: 1572 3832 ApaLI G'TGCA_C Cuts at: 3461 4617 3461 Size: 1156 4248 ApoI r'AATT_y Cuts at: 1493 2713 2939 3091 5200 1493 Size: 1220 226 152 2109 1697 Fragments arranged by size: 2109 1697 1220 226 152 AseI AT'TA_AT Cuts at: 160 1032 3958 160 Size: 872 2926 1606 Fragments arranged by size: 2926 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2729 1870 Size: 73 176 610 4545 Fragments arranged by size: 4545 610 176 73 BamHI G'GATC_C Cuts at: 2734 3101 2734 Size: 367 5037 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2725 4180 618 Size: 325 217 627 938 1455 1842 Fragments arranged by size: 1842 1455 938 627 325 217 BanII G_rGCy'C Cuts at: 1391 2165 2723 1391 Size: 774 558 4072 Fragments arranged by size: 4072 774 558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 2181 928 Size: 971 222 60 4151 Fragments arranged by size: 4151 971 222 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3595 3629 1903 Size: 34 1658 34 3678 Fragments arranged by size: 3678 1658 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3296 4506 3296 Size: 1210 4194 BclI T'GATC_A Cuts at: 1548 1548 Size: 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4015 136 Size: 107 122 71 631 605 2343 1525 Fragments arranged by size: 2343 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5399 5399 Size: 5404 BlpI GC'TnA_GC Cuts at: 2291 2821 2291 Size: 530 4874 Bme1580I G_kGCm'C Cuts at: 1790 3465 4621 1790 Size: 1675 1156 2573 Fragments arranged by size: 2573 1675 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5404 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 4093 442 Size: 416 53 83 399 24 1718 958 1753 Fragments arranged by size: 1753 1718 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 4064 1239 Size: 992 1833 2579 Fragments arranged by size: 2579 1833 992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2432 3529 4394 4692 2432 Size: 1097 865 298 3144 Fragments arranged by size: 3144 1097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4067 882 Size: 617 2568 2219 Fragments arranged by size: 2568 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2455 3195 493 Size: 711 1251 740 2702 Fragments arranged by size: 2702 1251 740 711 BsaBI GATnn'nnATC Cuts at: 5398 5398 Size: 5404 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3211 3593 4228 275 Size: 53 83 186 936 1678 382 635 1451 Fragments arranged by size: 1678 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3837 4235 4509 4656 5067 3837 Size: 398 274 147 411 4174 Fragments arranged by size: 4174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5179 Fragments arranged by size: 5179 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2077 2228 2231 2249 1186 Size: 891 151 3 18 4341 Fragments arranged by size: 4341 891 151 18 3 BseYI C'CCAG_C Cuts at: 2278 2295 4607 5103 2278 Size: 17 2312 496 2579 Fragments arranged by size: 2579 2312 496 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4831 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2276 2854 3615 4643 1540 Size: 275 186 275 578 761 1028 2301 Fragments arranged by size: 2301 1028 761 578 275 275 186 BsiHKAI G_wGCw'C Cuts at: 2165 2261 2438 3465 3550 4621 2165 Size: 96 177 1027 85 1071 2948 Fragments arranged by size: 2948 1071 1027 177 96 85 BsmI GAATG_Cn' Cuts at: 2915 3008 2915 Size: 93 5311 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2261 2438 2723 3465 3550 4621 Size: 399 375 96 177 285 742 85 1071 Cuts at: 4621 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 375 285 177 96 85 BspEI T'CCGG_A Cuts at: 5067 5067 Size: 5404 BspHI T'CATG_A Cuts at: 3293 5023 3293 Size: 1730 3674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2759 844 Size: 1915 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3291 1394 Size: 429 1468 3507 Fragments arranged by size: 3507 1468 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3899 4073 65 Size: 1183 2651 174 1396 Fragments arranged by size: 2651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2268 4048 4235 1700 Size: 110 458 1780 187 2869 Fragments arranged by size: 2869 1780 458 187 110 BsrGI T'GTAC_A Cuts at: 96 2457 96 Size: 2361 3043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5404 BssSI C'ACGA_G Cuts at: 3464 4476 3464 Size: 1012 4392 BstAPI GCAn_nnn'nTGC Cuts at: 794 2040 794 Size: 1246 4158 BstBI TT'CG_AA Cuts at: 4208 4208 Size: 5404 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5404 BstYI r'GATC_y Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399 Size: 762 367 385 17 1452 86 12 346 Cuts at: 5399 1972 Size: 1977 Fragments arranged by size: 1977 1452 762 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3913 Fragments arranged by size: 3913 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4665 Fragments arranged by size: 4665 683 56 BtsI GCAGTG_nn' Cuts at: 2920 3714 3734 2920 Size: 794 20 4590 Fragments arranged by size: 4590 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5404 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5369 DraI TTT'AAA Cuts at: 2708 2708 Size: 5404 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4411 809 Size: 507 3095 1802 Fragments arranged by size: 3095 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2273 2301 Size: 54 1425 50 275 18 357 86 28 Cuts at: 2301 2851 3740 5369 8 Size: 550 889 1629 43 Fragments arranged by size: 1629 1425 889 550 357 275 86 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2273 2851 1537 Size: 275 461 578 4090 Fragments arranged by size: 4090 578 461 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3334 1353 Size: 1981 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3991 4365 4511 387 Size: 1064 138 2402 374 146 1280 Fragments arranged by size: 2402 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2163 2163 Size: 5404 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 3467 4064 4851 5119 1239 Size: 393 599 1236 597 787 268 1524 Fragments arranged by size: 1524 1236 787 599 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2541 2541 Size: 5404 EcoRI G'AATT_C Cuts at: 2713 3091 2713 Size: 378 5026 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2512 2512 Size: 5404 FspI TGC'GCA Cuts at: 3910 3910 Size: 5404 HaeII r_GCGC'y Cuts at: 1678 4551 1678 Size: 2873 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2657 2689 4045 4077 4119 Size: 32 135 32 1085 32 1356 32 42 Cuts at: 4119 4151 1373 Size: 32 2626 Fragments arranged by size: 2626 1356 1085 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2748 2994 669 Size: 988 281 810 246 3079 Fragments arranged by size: 3079 988 810 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5404 HpaI GTT'AAC Cuts at: 2994 2994 Size: 5404 KpnI G_GTAC'C Cuts at: 2729 2729 Size: 5404 MfeI C'AATT_G Cuts at: 3003 3003 Size: 5404 MluI A'CGCG_T Cuts at: 2178 5280 2178 Size: 3102 2302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4518 4702 1305 Size: 3213 184 2007 Fragments arranged by size: 3213 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 10 Size: 54 1425 343 471 3111 Fragments arranged by size: 3111 1425 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2026 3362 3721 3880 518 Size: 648 860 1336 359 159 2042 Fragments arranged by size: 2042 1336 860 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 2209 2245 2278 2820 3497 4645 4890 Size: 528 75 36 33 542 677 1148 245 Cuts at: 4890 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 542 528 245 75 36 33 NaeI GCC'GGC Cuts at: 2270 2270 Size: 5404 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3913 Fragments arranged by size: 3913 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5404 NgoMIV G'CCGG_C Cuts at: 2268 2268 Size: 5404 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5404 NotI GC'GGCC_GC Cuts at: 2273 2851 2273 Size: 578 4826 NspI r_CATG'y Cuts at: 1132 1229 2158 2762 4307 1132 Size: 97 929 604 1545 2229 Fragments arranged by size: 2229 1545 929 604 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5404 PciI A'CATG_T Cuts at: 4303 4303 Size: 5404 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5404 PmeI GTTT'AAAC Cuts at: 2708 2708 Size: 5404 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3505 4986 5018 3473 Size: 32 1481 32 3859 Fragments arranged by size: 3859 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2541 2541 Size: 5404 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5404 PsiI TTA'TAA Cuts at: 2974 2974 Size: 5404 PspXI vC'TCGA_Gb Cuts at: 2119 2119 Size: 5404 PstI C_TGCA'G Cuts at: 830 2756 830 Size: 1926 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5404 PvuII CAG'CTG Cuts at: 2134 2209 2134 Size: 75 5329 SacI G_AGCT'C Cuts at: 2165 2165 Size: 5404 SalI G'TCGA_C Cuts at: 1936 2746 1936 Size: 810 4594 SbfI CC_TGCA'GG Cuts at: 2756 2756 Size: 5404 ScaI AGT'ACT Cuts at: 3652 5364 3652 Size: 1712 3692 SfcI C'TryA_G Cuts at: 826 1045 2752 3887 4568 4759 826 Size: 219 1707 1135 681 191 1471 Fragments arranged by size: 1707 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5404 SmaI CCC'GGG Cuts at: 1872 2731 1872 Size: 859 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2119 2411 3508 4409 4671 820 Size: 197 926 176 292 1097 901 262 1553 Fragments arranged by size: 1553 1097 926 901 292 262 197 176 SnaBI TAC'GTA Cuts at: 493 3195 493 Size: 2702 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5404 SphI G_CATG'C Cuts at: 1132 2762 1132 Size: 1630 3774 SspI AAT'ATT Cuts at: 5 52 3328 5 Size: 47 3276 2081 Fragments arranged by size: 3276 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5404 StyI C'CwwG_G Cuts at: 513 1065 2004 2843 513 Size: 552 939 839 3074 Fragments arranged by size: 3074 939 839 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2007 2287 3431 3448 3601 3786 4231 Size: 1255 427 280 1144 17 153 185 445 Cuts at: 4231 325 Size: 1498 Fragments arranged by size: 1498 1255 1144 445 427 280 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2457 3650 5362 Size: 274 80 33 51 1443 480 1193 1712 Cuts at: 5362 96 Size: 138 Fragments arranged by size: 1712 1443 1193 480 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5364 5396 5364 Size: 32 5372 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5404 XbaI T'CTAG_A Cuts at: 2740 2740 Size: 5404 XhoI C'TCGA_G Cuts at: 1943 2119 1943 Size: 176 5228 XmaI C'CCGG_G Cuts at: 1870 2729 1870 Size: 859 4545 XmnI GAAnn'nnTTC Cuts at: 3533 3533 Size: 5404 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3212 276 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI